FastQCFastQC Report
Sat 18 Jun 2016
SRR3548330_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548330_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787736
Sequences flagged as poor quality0
Sequence length46
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA474886.0284156113215595No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA117791.4952979170686627No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86211.0944021855037729No Hit
GGAAGAGGGGTCCATTATCTTTTCTATGCTTGGGATTTTCTTTTTA60550.7686585353468675No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48750.6188621568647364No Hit
CTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTT40830.5183208587648653No Hit
TAACTTGGGAACTGGTGTTCTATTTTTTTTTAAAGGGGGAAAATTT30640.38896279971970305No Hit
GGATTAGGGTGATTTTCTTCCATTAAAATCTAATTAAAATATTTTG14630.18572212010115063No Hit
TACAAAGGGGAGGGTTAGGAATCAAACTCTAGAGGAACAGTAAATA13090.16617242324839793No Hit
GTTGAAGGGCATATTGTGAATCATTTTGAAGTTCATTATAGCGATA12630.160332903409264No Hit
AGTTCTGGGCACATAGTTTTATAACTCTATGATCTATGAGAAAACT11920.15131973148364428No Hit
ACACCCGGGAAGCTTTTTTAGTTCCTCTTTCAAACACAGTACACAT11910.15119278540018483No Hit
TCTATTGGGGGTAAAGACAAATACTTATTTTCATTCTAAGAGATTG11790.14966943239867164No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGT11640.14776524114678014No Hit
CCTCGCAGGGGAGTTTATACTTTTAAATAAATGGTAATATTTTCTC11380.14446464297683487No Hit
TAACGAGGGGGAAAATGGAACATGAAAAATGAGATATGCACATTGT11330.1438299125595377No Hit
AATCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGT11180.14192572130764622No Hit
GCTAGAGGGAGGGGAGGATACTTTCATTATTGTGGTTTGTAGTGTT11020.1398945839722953No Hit
TAACTGGGGGAGGTATGCAAGGAAATCCTTTTGGAAAATCACTGCT10940.13887901530461982No Hit
TACACGGGAGCTGGTTAAATGTTTTGACTCTAAATTGTTTTCATCT10900.13837123097078208No Hit
TATCAGGGGTAACAAATATATTTTTTTTTCCTTTTGAAAACAGAAA10840.13760955447002549No Hit
ACCAACGGGTACAGTCCCTTATGTATATAGCTTTTTTTTCTCTTGA10780.1368478779692689No Hit
CTTCTTGGGTACTATTTTTAAAATAATCCATGATTAGACAGAGAAA10710.13595925538505285No Hit
CGCAACGGGAAATATTTTTTTAATATTAGCCTTCTAGTCTGTAATG10710.13595925538505285No Hit
CTTACAGAAGTACAGACGTTATCAAGTTGTTATGTTTTATTCCCCC10700.13583230930159343No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTAT10580.13430895630008022No Hit
ATGGACGGGAAGAAATCATATTTTTGCATTTCAGTCAAACTAGTAA10570.1341820102166208No Hit
TAAAGAGGGATTTTTGTAAATGAAGTTATGTTAAGGGAAAATATAT10300.1307544659632161No Hit
AAATCTGGGACAAAATTTTTATTTTCTTTTTATTGTCTTTTAGAGT10260.13024668162937836No Hit
CAACTCGGGAAATGAGTTGAGAAAAGTTGACCATGCACTATTTATA10210.1296119512120812No Hit
ATAACAGGGATCAATATTTTCACAACCTCAATCTTATTAATCTTCA9960.12643829912559537No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9920.12593051479175765No Hit
AACTTTGGGAGTGATCAGTCATTTCATTTTTTTTTTTCAAGCAAGG9750.12377243137294729No Hit
GAGACAGGGATATGTATTTTGTAATTTTTTGTTATGTTTAAATCTT9720.12339159312256898No Hit
GCTACTGGCCTCTAAATACAACCCTTCTTTACAAAAACAAAAAACA9690.1230107548721907No Hit
TGCAAAGGGCAAATTCTTTTTTATTATTATTTATTTGATTAGGTAT9300.11805985761727279No Hit
TACAGAGGAAAAGGCAAAGAGAAGAAGAGAGAAGAAAATGGAAAGA9190.11666345069921903No Hit
AAACTAGGAAAGGGGATAACATTTGAAATGTAAATAAAGAAAATGT9180.1165365046157596No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8980.11399758294657093No Hit
AAACTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGT8940.11348979861273321No Hit
AGTACCGGGTAGGAAAATATCTATAGTTTTGTGATTGTCAGTTTGG8930.11336285252927378No Hit
TATCCCGGGAGAAATAAAAGACACATATTTTTGCATCTATACGGCA8880.11272812211197661No Hit
ATAGTAGGGGATAAGTTTTTAATTAAAAAGATTGAGCAGAAAAAAG8830.11209339169467943No Hit
ATGTCAGGGGAATTTTAGTCAACATTCCTCTCATTTGTATATGTAT8710.11057003869316623No Hit
AGTTCAGGGGGTTTGCAATAAACTGAAGAATTGTTTTACCCCTTTG8540.10841195527435588No Hit
TCAGACGGGAAAGCCTAATTTTACTTTTCTAATTCTGGTAGCTATT8530.10828500919089644No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8360.10612692577208609No Hit
AGCTACGGGAAAGTAGAAAAACATGCAATAATATACAAAGTATAAT8330.10574608752170778No Hit
ATCAGAGGGAGGGGGATGTAGGGTGATAGCATACTAAGAATGGGAA8230.10447662668711345No Hit
CGAAACGGGATTGGACAAAGTTCTTCCCCCTCTCATTAAAAAAGAC8230.10447662668711345No Hit
AAGAAGGAAGAAAGAAGAAGAAGAAAGAAAAGAAGAAGAAGAAAGA8120.10308021976905969No Hit
AAAACAGGGGGTATTGGGCATAATTGTCTTTATCCATCGATCTTTA8090.10269938151868138No Hit
AGGCTTGGGAATTAATTGAATTTTGAGTTGATTGGAGTCTTTCTGT8020.10181075893446534No Hit
TTATGAGGGTCTTACAGACTTGTTTACTTTTAAAAGATATTTTATG8010.10168381285100593No Hit
CACCTAGGGGAATTTTTCTCTTTCTCACTGATTTTCAGTAATTTGG7930.10066824418333047No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCATT200.001255528140.00000427
GTGCACC200.001255528140.00000417
AACGTAG401.9270374E-840.0000041
TCGTTGA200.001255528140.00000424
GGTACGT200.001255528140.00000437
TACCCGC800.040.00000425
AATTTCG200.001255528140.00000439
ATGATCA401.9270374E-840.00000417
CGAGTAA200.001255528140.00000425
TTTACGG200.001255528140.0000042
CGAAAGC200.001255528140.00000432
CCCTAGA401.9270374E-840.00000426
AGCTTAC200.001255528140.00000435
GATCCGG401.9270374E-840.0000042
TTGTCCG800.040.00000425
GCACGAC200.001255528140.00000435
GCGCCCT200.001255528140.00000425
ATGCTCG200.001255528140.0000041
TACGGTA200.001255528140.00000410
TGCGGCT200.001255528140.00000412
Produced by FastQC (version 0.11.5)