##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548330_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	787736
Sequences flagged as poor quality	0
Sequence length	46
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74082941493089	33.0	31.0	34.0	30.0	34.0
2	31.98738663714747	33.0	31.0	34.0	30.0	34.0
3	32.14340210425828	34.0	31.0	34.0	30.0	34.0
4	35.88702433302528	37.0	35.0	37.0	35.0	37.0
5	35.58641854631501	37.0	35.0	37.0	33.0	37.0
6	35.64361283475682	37.0	35.0	37.0	33.0	37.0
7	35.57359191404227	37.0	35.0	37.0	33.0	37.0
8	35.87350330567601	37.0	35.0	37.0	35.0	37.0
9	37.33813358790255	39.0	37.0	39.0	34.0	39.0
10	36.83047493068744	39.0	37.0	39.0	32.0	39.0
11	36.97101440076371	39.0	37.0	39.0	33.0	39.0
12	36.6541290483106	39.0	35.0	39.0	32.0	39.0
13	36.50605278925934	39.0	35.0	39.0	32.0	39.0
14	37.70326352991358	40.0	36.0	41.0	33.0	41.0
15	37.594369179522076	39.0	36.0	41.0	33.0	41.0
16	37.51363147044187	39.0	36.0	41.0	32.0	41.0
17	37.42465115216265	39.0	36.0	40.0	33.0	41.0
18	37.20188489544721	38.0	36.0	40.0	33.0	41.0
19	37.18327967745539	39.0	35.0	40.0	32.0	41.0
20	37.04854037393238	39.0	35.0	40.0	32.0	41.0
21	37.08130261915159	39.0	35.0	40.0	32.0	41.0
22	37.117054444636274	39.0	35.0	40.0	33.0	41.0
23	36.96735581463841	39.0	35.0	40.0	32.0	41.0
24	36.68160145023206	38.0	35.0	40.0	32.0	41.0
25	36.77552631846202	38.0	35.0	40.0	32.0	41.0
26	36.722544101069396	38.0	35.0	40.0	32.0	41.0
27	36.65355906039587	38.0	35.0	40.0	32.0	41.0
28	36.64110311068683	38.0	35.0	40.0	32.0	41.0
29	36.425339199935	38.0	35.0	40.0	31.0	41.0
30	36.41976753633197	38.0	35.0	40.0	31.0	41.0
31	36.350378807113046	38.0	35.0	40.0	31.0	41.0
32	36.258248956503195	38.0	35.0	40.0	31.0	41.0
33	36.211579767841	38.0	35.0	40.0	31.0	41.0
34	36.10398788426579	38.0	35.0	40.0	31.0	41.0
35	35.89356586470594	38.0	35.0	40.0	30.0	41.0
36	35.892700092416746	38.0	35.0	40.0	30.0	41.0
37	35.85884991926229	38.0	35.0	40.0	30.0	41.0
38	35.80213421755512	38.0	35.0	40.0	30.0	41.0
39	35.71502889292859	38.0	35.0	40.0	30.0	41.0
40	35.5138967877563	38.0	35.0	40.0	29.0	41.0
41	35.478808890288114	38.0	35.0	40.0	29.0	41.0
42	35.38001437029665	38.0	35.0	40.0	28.0	41.0
43	35.310376826754144	38.0	35.0	40.0	28.0	41.0
44	35.243818995196364	37.0	34.0	40.0	28.0	41.0
45	35.11861334254115	37.0	34.0	40.0	27.0	41.0
46	34.28447220896341	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	3.0
10	16.0
11	18.0
12	11.0
13	16.0
14	20.0
15	55.0
16	86.0
17	172.0
18	290.0
19	554.0
20	888.0
21	1475.0
22	2235.0
23	3354.0
24	4292.0
25	5630.0
26	6970.0
27	8170.0
28	9926.0
29	12264.0
30	15013.0
31	19169.0
32	25712.0
33	37060.0
34	62016.0
35	96144.0
36	81619.0
37	95596.0
38	153944.0
39	145012.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.692084657804138	35.994165558004205	19.483430997186872	23.830318787004785
2	24.026323539866148	37.341952126093005	18.15519412595083	20.476530208090022
3	17.130231448099366	38.471772268881956	21.00792651345121	23.39006976956747
4	17.311129617029057	40.260823423075756	18.22577614835427	24.20227081154092
5	16.695695004417725	40.61703413326292	17.05342906760641	25.633841794712946
6	16.90718717946114	46.16127738227021	18.19949830907817	18.73203712919049
7	75.88582977037991	16.28032741933846	4.722140412523993	3.1117023977576244
8	77.22346065179198	14.836950450404704	4.35323509399088	3.586353803812445
9	72.53178729929824	17.55143855301776	6.643342439599054	3.273431708084942
10	33.9882904932617	39.6639229386495	12.094153371180193	14.253633196908607
11	27.04675170361644	38.638452476464195	20.11803446840058	14.196761351518782
12	24.51582763768572	36.28855352554663	24.05704449206333	15.138574344704317
13	20.42917931895965	40.703865254349175	23.32710451217159	15.539850914519585
14	16.802456660607106	42.17339311647557	26.743731402398772	14.28041882051855
15	14.597022352666375	39.15537692831101	31.03933297449907	15.208267744523546
16	16.318665136543206	36.832644439253755	30.420597763717794	16.428092660485238
17	16.379726202687195	36.813856418901764	26.8591253922634	19.947291986147643
18	17.676099606974926	35.387109386901194	29.957244559090864	16.979546447033016
19	17.527699635410848	37.89404064305808	27.760569530908835	16.81769019062224
20	19.05003706825637	38.29772918845908	25.755963927000924	16.896269816283628
21	17.326870931378025	39.3497313820874	27.096133730082162	16.227263956452415
22	17.45534036783897	35.95684340946713	28.26518021266008	18.32263601003382
23	16.164679537306913	37.91295560949354	26.918409213238952	19.003955639960594
24	15.179704875745173	35.91266617242324	30.892329409853048	18.01529954197853
25	16.261031614652623	37.949769973696775	27.9290269836595	17.860171427991105
26	17.33232961296678	41.39267470320006	25.27966222186113	15.99533346197203
27	14.721810352707	38.99161648064834	27.35789655417551	18.928676612469154
28	15.130957579696751	38.24948967674449	30.251251688382908	16.368301055175845
29	16.142337026618055	38.406014197649974	27.733530015131976	17.7181187606
30	16.84168300039607	37.24229945057735	28.408248448718858	17.50776910030772
31	19.454868128408503	37.2718778880234	26.078533925071344	17.194720058496753
32	19.39913879776981	37.50520478942184	25.949048919942726	17.14660749286563
33	18.61575451674165	38.344445347172154	25.501944813998602	17.5378553220876
34	17.517670894817552	37.05086475672053	26.919551727990086	18.511912620471833
35	17.7315750454467	37.74843348533011	27.725278519707107	16.79471294951608
36	19.13090172342003	37.86573166644663	26.698665542770673	16.30470106736267
37	18.497821605207836	38.08585617516528	25.779195060274002	17.63712715935288
38	17.18672245523881	38.16799029116354	28.00595631023592	16.639330943361735
39	17.005824286309117	35.87229731788315	27.920267703900798	19.201610691906936
40	19.711553109163475	35.79790691297592	24.802598840220583	19.68794113764002
41	16.478236363451714	36.73616541582459	26.823453542811297	19.9621446779124
42	20.301217666832542	35.489300984086036	24.8128814729808	19.396599876100623
43	18.762250297053836	35.53347822112992	26.87169305452588	18.83257842729036
44	18.018980978398854	34.80366518732164	27.490174373140242	19.687179461139266
45	19.48419267368763	35.44690099221059	26.99889303015223	18.070013303949548
46	17.443280489910325	36.483034925406486	28.48682807438025	17.586856510302944
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	55444.0
1	41475.5
2	27507.0
3	16397.5
4	5288.0
5	4296.0
6	3304.0
7	3304.0
8	3098.5
9	2893.0
10	2981.5
11	3070.0
12	3522.5
13	3975.0
14	3789.5
15	3604.0
16	3883.5
17	4163.0
18	3907.0
19	3651.0
20	3651.0
21	6486.0
22	9321.0
23	11033.0
24	12745.0
25	12612.5
26	12480.0
27	13310.0
28	14140.0
29	18984.5
30	23829.0
31	23829.0
32	25915.5
33	28002.0
34	32441.0
35	36880.0
36	45029.5
37	53179.0
38	45829.5
39	38480.0
40	38760.5
41	39041.0
42	41965.0
43	44889.0
44	44889.0
45	50007.5
46	55126.0
47	56981.0
48	58836.0
49	57470.0
50	56104.0
51	49514.0
52	42924.0
53	38771.0
54	34618.0
55	30492.5
56	26367.0
57	26367.0
58	23414.0
59	20461.0
60	19923.5
61	19386.0
62	16988.0
63	14590.0
64	13050.0
65	11510.0
66	9381.5
67	7253.0
68	5720.5
69	4188.0
70	4188.0
71	3998.0
72	3808.0
73	3451.0
74	3094.0
75	2441.0
76	1788.0
77	1376.0
78	964.0
79	670.5
80	377.0
81	377.0
82	299.0
83	221.0
84	205.0
85	189.0
86	107.0
87	25.0
88	18.0
89	11.0
90	7.5
91	4.0
92	3.0
93	2.0
94	2.0
95	3.0
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	787736.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.065283671908357
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.44416522603137	13.629202461985809
2	10.431411366618576	3.768928108726676
3	3.7117556047304054	2.0116175376075156
4	1.9060754130096909	1.377351721442798
5	1.0782364045076418	0.9739323256404537
6	0.7369474306282185	0.7987898631349664
7	0.5663106657621618	0.7161393976394514
8	0.4255437781080639	0.6150055253070239
9	0.2935126991727252	0.4772151154676505
>10	3.428606582696799	14.579523274507578
>50	1.2578545485670112	16.379016685651788
>100	0.6186979467713228	20.560369946981325
>500	0.07971820750873373	9.843238719216886
>1k	0.018342242435637854	4.845980566576072
>5k	0.0014109417258182963	1.870387407823228
>10k+	0.0014109417258182963	7.553301342290765
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47488	6.0284156113215595	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11779	1.4952979170686627	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8621	1.0944021855037729	No Hit
GGAAGAGGGGTCCATTATCTTTTCTATGCTTGGGATTTTCTTTTTA	6055	0.7686585353468675	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4875	0.6188621568647364	No Hit
CTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTT	4083	0.5183208587648653	No Hit
TAACTTGGGAACTGGTGTTCTATTTTTTTTTAAAGGGGGAAAATTT	3064	0.38896279971970305	No Hit
GGATTAGGGTGATTTTCTTCCATTAAAATCTAATTAAAATATTTTG	1463	0.18572212010115063	No Hit
TACAAAGGGGAGGGTTAGGAATCAAACTCTAGAGGAACAGTAAATA	1309	0.16617242324839793	No Hit
GTTGAAGGGCATATTGTGAATCATTTTGAAGTTCATTATAGCGATA	1263	0.160332903409264	No Hit
AGTTCTGGGCACATAGTTTTATAACTCTATGATCTATGAGAAAACT	1192	0.15131973148364428	No Hit
ACACCCGGGAAGCTTTTTTAGTTCCTCTTTCAAACACAGTACACAT	1191	0.15119278540018483	No Hit
TCTATTGGGGGTAAAGACAAATACTTATTTTCATTCTAAGAGATTG	1179	0.14966943239867164	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGT	1164	0.14776524114678014	No Hit
CCTCGCAGGGGAGTTTATACTTTTAAATAAATGGTAATATTTTCTC	1138	0.14446464297683487	No Hit
TAACGAGGGGGAAAATGGAACATGAAAAATGAGATATGCACATTGT	1133	0.1438299125595377	No Hit
AATCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGT	1118	0.14192572130764622	No Hit
GCTAGAGGGAGGGGAGGATACTTTCATTATTGTGGTTTGTAGTGTT	1102	0.1398945839722953	No Hit
TAACTGGGGGAGGTATGCAAGGAAATCCTTTTGGAAAATCACTGCT	1094	0.13887901530461982	No Hit
TACACGGGAGCTGGTTAAATGTTTTGACTCTAAATTGTTTTCATCT	1090	0.13837123097078208	No Hit
TATCAGGGGTAACAAATATATTTTTTTTTCCTTTTGAAAACAGAAA	1084	0.13760955447002549	No Hit
ACCAACGGGTACAGTCCCTTATGTATATAGCTTTTTTTTCTCTTGA	1078	0.1368478779692689	No Hit
CTTCTTGGGTACTATTTTTAAAATAATCCATGATTAGACAGAGAAA	1071	0.13595925538505285	No Hit
CGCAACGGGAAATATTTTTTTAATATTAGCCTTCTAGTCTGTAATG	1071	0.13595925538505285	No Hit
CTTACAGAAGTACAGACGTTATCAAGTTGTTATGTTTTATTCCCCC	1070	0.13583230930159343	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTAT	1058	0.13430895630008022	No Hit
ATGGACGGGAAGAAATCATATTTTTGCATTTCAGTCAAACTAGTAA	1057	0.1341820102166208	No Hit
TAAAGAGGGATTTTTGTAAATGAAGTTATGTTAAGGGAAAATATAT	1030	0.1307544659632161	No Hit
AAATCTGGGACAAAATTTTTATTTTCTTTTTATTGTCTTTTAGAGT	1026	0.13024668162937836	No Hit
CAACTCGGGAAATGAGTTGAGAAAAGTTGACCATGCACTATTTATA	1021	0.1296119512120812	No Hit
ATAACAGGGATCAATATTTTCACAACCTCAATCTTATTAATCTTCA	996	0.12643829912559537	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	992	0.12593051479175765	No Hit
AACTTTGGGAGTGATCAGTCATTTCATTTTTTTTTTTCAAGCAAGG	975	0.12377243137294729	No Hit
GAGACAGGGATATGTATTTTGTAATTTTTTGTTATGTTTAAATCTT	972	0.12339159312256898	No Hit
GCTACTGGCCTCTAAATACAACCCTTCTTTACAAAAACAAAAAACA	969	0.1230107548721907	No Hit
TGCAAAGGGCAAATTCTTTTTTATTATTATTTATTTGATTAGGTAT	930	0.11805985761727279	No Hit
TACAGAGGAAAAGGCAAAGAGAAGAAGAGAGAAGAAAATGGAAAGA	919	0.11666345069921903	No Hit
AAACTAGGAAAGGGGATAACATTTGAAATGTAAATAAAGAAAATGT	918	0.1165365046157596	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	898	0.11399758294657093	No Hit
AAACTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGT	894	0.11348979861273321	No Hit
AGTACCGGGTAGGAAAATATCTATAGTTTTGTGATTGTCAGTTTGG	893	0.11336285252927378	No Hit
TATCCCGGGAGAAATAAAAGACACATATTTTTGCATCTATACGGCA	888	0.11272812211197661	No Hit
ATAGTAGGGGATAAGTTTTTAATTAAAAAGATTGAGCAGAAAAAAG	883	0.11209339169467943	No Hit
ATGTCAGGGGAATTTTAGTCAACATTCCTCTCATTTGTATATGTAT	871	0.11057003869316623	No Hit
AGTTCAGGGGGTTTGCAATAAACTGAAGAATTGTTTTACCCCTTTG	854	0.10841195527435588	No Hit
TCAGACGGGAAAGCCTAATTTTACTTTTCTAATTCTGGTAGCTATT	853	0.10828500919089644	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	836	0.10612692577208609	No Hit
AGCTACGGGAAAGTAGAAAAACATGCAATAATATACAAAGTATAAT	833	0.10574608752170778	No Hit
ATCAGAGGGAGGGGGATGTAGGGTGATAGCATACTAAGAATGGGAA	823	0.10447662668711345	No Hit
CGAAACGGGATTGGACAAAGTTCTTCCCCCTCTCATTAAAAAAGAC	823	0.10447662668711345	No Hit
AAGAAGGAAGAAAGAAGAAGAAGAAAGAAAAGAAGAAGAAGAAAGA	812	0.10308021976905969	No Hit
AAAACAGGGGGTATTGGGCATAATTGTCTTTATCCATCGATCTTTA	809	0.10269938151868138	No Hit
AGGCTTGGGAATTAATTGAATTTTGAGTTGATTGGAGTCTTTCTGT	802	0.10181075893446534	No Hit
TTATGAGGGTCTTACAGACTTGTTTACTTTTAAAAGATATTTTATG	801	0.10168381285100593	No Hit
CACCTAGGGGAATTTTTCTCTTTCTCACTGATTTTCAGTAATTTGG	793	0.10066824418333047	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5389216691886624E-4	0.0	0.0	0.5677028852305849	0.0
2	2.5389216691886624E-4	0.0	0.0	0.7404765048188733	0.0
3	2.5389216691886624E-4	0.0	0.0	0.9641555038743944	0.0
4	2.5389216691886624E-4	0.0	0.0	2.033549310936659	0.0
5	2.5389216691886624E-4	0.0	0.0	2.1644307229833344	0.0
6	2.5389216691886624E-4	0.0	0.0	2.9230605177369067	0.0
7	2.5389216691886624E-4	0.0	0.0	3.4432855677536636	0.0
8	2.5389216691886624E-4	0.0	0.0	3.745290300303655	0.0
9	2.5389216691886624E-4	0.0	0.0	4.389414727776819	0.0
10	2.5389216691886624E-4	0.0	0.0	5.2054241522540545	0.0
11	2.5389216691886624E-4	0.0	0.0	6.331816750789605	0.0
12	2.5389216691886624E-4	0.0	0.0	6.770542415225406	0.0
13	2.5389216691886624E-4	0.0	0.0	6.941031005311424	0.0
14	2.5389216691886624E-4	0.0	0.0	7.151634557770624	0.0
15	2.5389216691886624E-4	0.0	0.0	7.330755481531884	0.0
16	2.5389216691886624E-4	0.0	0.0	7.733555404348665	0.0
17	2.5389216691886624E-4	0.0	0.0	8.19589304030792	0.0
18	2.5389216691886624E-4	0.0	0.0	8.739857007931592	0.0
19	2.5389216691886624E-4	0.0	0.0	9.018884499375424	0.0
20	2.5389216691886624E-4	0.0	0.0	9.266048523870941	0.0
21	2.5389216691886624E-4	0.0	0.0	9.599916723369251	0.0
22	2.5389216691886624E-4	0.0	0.0	9.953334619720312	0.0
23	2.5389216691886624E-4	0.0	0.0	10.326809997257964	0.0
24	2.5389216691886624E-4	0.0	0.0	10.586287791849045	0.0
25	2.5389216691886624E-4	0.0	0.0	10.801334457229325	0.0
26	2.5389216691886624E-4	0.0	0.0	11.016000284359228	0.0
27	2.5389216691886624E-4	0.0	0.0	11.246280479754638	0.0
28	2.5389216691886624E-4	0.0	0.0	11.45637624788	0.0
29	2.5389216691886624E-4	0.0	0.0	11.689068418861142	0.0
30	3.8083825037829935E-4	0.0	0.0	11.984725847238161	0.0
31	3.8083825037829935E-4	0.0	0.0	12.21322879746514	0.0
32	3.8083825037829935E-4	0.0	0.0	12.436146120019906	0.0
33	3.8083825037829935E-4	0.0	0.0	12.677724516843206	0.0
34	3.8083825037829935E-4	0.0	0.0	12.9077508200717	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCATT	20	0.0012555281	40.000004	27
GTGCACC	20	0.0012555281	40.000004	17
AACGTAG	40	1.9270374E-8	40.000004	1
TCGTTGA	20	0.0012555281	40.000004	24
GGTACGT	20	0.0012555281	40.000004	37
TACCCGC	80	0.0	40.000004	25
AATTTCG	20	0.0012555281	40.000004	39
ATGATCA	40	1.9270374E-8	40.000004	17
CGAGTAA	20	0.0012555281	40.000004	25
TTTACGG	20	0.0012555281	40.000004	2
CGAAAGC	20	0.0012555281	40.000004	32
CCCTAGA	40	1.9270374E-8	40.000004	26
AGCTTAC	20	0.0012555281	40.000004	35
GATCCGG	40	1.9270374E-8	40.000004	2
TTGTCCG	80	0.0	40.000004	25
GCACGAC	20	0.0012555281	40.000004	35
GCGCCCT	20	0.0012555281	40.000004	25
ATGCTCG	20	0.0012555281	40.000004	1
TACGGTA	20	0.0012555281	40.000004	10
TGCGGCT	20	0.0012555281	40.000004	12
>>END_MODULE