FastQCFastQC Report
Sat 18 Jun 2016
SRR3548329_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548329_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729454
Sequences flagged as poor quality0
Sequence length46
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA439446.02423182270575No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA109711.504001623131822No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTAT107231.4700035917275112No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGT96291.3200284048068829No Hit
AATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGT87631.2013094725644113No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA77701.065180258110861No Hit
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTT57080.7825030776443751RNA PCR Primer, Index 29 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52000.7128619488000614No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACTA27530.37740556635510947No Hit
AATGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCC26230.3595840176351079No Hit
AATGATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGC18860.25854954527632995No Hit
AACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTC17430.23894584168432825No Hit
AGCACTGGGATTCTATATTTAATTAGGTGTTTTAATCAAGATATTT16430.22523695805355787No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCACTAACCT15670.21481820649417235No Hit
ACACGGGTGTAAATTAAACCTTACGATGGGAAAGACAAAGTTTAGT15160.20782667584247944No Hit
AAAGCAGGGGATAGAGTTGTTCTGTTAATTTTACTTTTAGTGTTTT14230.19507741406586296No Hit
GTTAGAGGGGTAAGTAAATAGTTTTATTTCTTTTTGTATTAAACTT13090.1794492867267847No Hit
TAACATGGGAGGAATATTCAGGTTATTTAACATTGTTTTTAAATGG12430.17040142353047621No Hit
GCATGGGGTTTATAAAATAGATTCTCCTTACTGATAGGTTTGTGTG12410.1701272458578608No Hit
TTCCTTGGGAATGTTCACTTAAAAAGACAGATTTTGGAGATTGTAT12270.16820800214955295No Hit
AAACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGT12210.1673854691317067No Hit
TAGCGCGGGATTTTTGGTCCTTGGTTTAATTATCTTCAAAATACAT11340.15545874037293647No Hit
AAGCAGGAGACTCCTTAATACGTATACATCATGCATTGCTATTGAT10750.14737049903078192No Hit
GGCTATGGGACAATAACACTTATTTCTTAGAAAGAAACACAGGTTT10500.14394327812308932No Hit
AGCAAGGGAGATCTTTCCATCTTCTGAGATCTTCTTTAATTTCTTT10480.14366910045047393No Hit
AGACCAGGGGTGGATTTAATGAATTTTTAGGTGAAAGGATGTTTTA10420.1428465674326277No Hit
GCTCTTGGGCATTTTATATTATGGTACTTTAATCACTGTTTTTTCT10170.1394193465249351No Hit
GCAAGTGCTTTGGGGTTTTTTATTAATATTAATTAGAACATTGATA10170.1394193465249351No Hit
GGGAGCGGGTCTTAAAATATAACTAAACTAATTTTAAAATTGCTTA10170.1394193465249351No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10080.13818554699816576No Hit
CGTTAAGGGATTTATTTGTTTATATAAGCATACAGTTTAAAATCAG9980.1368146586350887No Hit
CTGAATGGGAATGTAATTCTGCCACCTAAATACTATATGTTTTGGT9670.13256490470954987No Hit
CTGGAAGGGGTGATGATTTTTAAAACTGTCTTTTAAATTTATGTAA9570.13119401634647285No Hit
GGCTGGGGGATACTATGCCACCTACTCTTTCTGATCCTTAAGAAAA9200.12612172940308777No Hit
GGTGGGGGGGATGAGACCACAAGATAGGAAAATGTACATTACTTCT8860.12146070896862586No Hit
ACGGGTATCACTAAATTGAGTGATACAAATTTCATCTATTTTATTA8820.12091235362339503No Hit
GAACAGGGTCTGTAAATGTCTGCACTAAGGGTTTCTTTCAGTTTGC8750.1199527317692411No Hit
CAAAAAGGGAAGTTAGGATTTGTTGCCATTGATTCCTTTTTATAAC8690.11913019875139487No Hit
CAAGCTGGGATCTTCCATTTTATTTAATTTTGTTCCTTTTTCTTTT8600.11789639922462553No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8490.11638842202524079No Hit
TAAACAGTTCTTTTCTCATACATTCTGTTTTTTATCAATATTCCCT8170.11200157926339427No Hit
GAAGGAGGGAAAGGAAAGGGATAGTGATAGAATCATACTTTAAGAA8030.11008233555508641No Hit
GGCAACGGGAGCTATACAAACCCATATATAATTATACATAATATAT7990.10953398020985558No Hit
AGCAATGGGCAGAATATGGAAACAAAACTAAACAGAGACACAGTGA7920.10857435835570166No Hit
TTTTTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAA7850.10761473650154774No Hit
AGGCGTGGGATTTCCATTTTTGTAAGTATTTTTCTGTGCATTGCAA7680.10528422628431676No Hit
ATCCACGGGATTTTTAGACCTCCCGTTTTTCTAATCTGGGCTATTT7580.10391333792123972No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7450.10213118304923957No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGC200.001255467340.022
ACGCGAT304.8650654E-640.021
AACGTTA200.001255467340.021
CGGGTTA200.001255467340.06
ACTATTA200.001255467340.015
AAGTACG304.8650654E-640.01
AAGTAAC555.456968E-1240.018
AACCGAC257.7918514E-540.034
TCGTTGA507.8216544E-1140.024
AGCCGAC257.7918514E-540.036
TCGTTAA304.8650654E-640.018
CCGGGTC304.8650654E-640.05
CGAACTA200.001255467340.033
CTCAACG257.7918514E-540.01
CAAGTCG304.8650654E-640.01
AGTCAAT850.040.012
TTTACGG257.7918514E-540.02
CGACGCA257.7918514E-540.037
GCGGTAC507.8216544E-1140.024
CCTAGAT200.001255467340.038
Produced by FastQC (version 0.11.5)