##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548329_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	729454
Sequences flagged as poor quality	0
Sequence length	46
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.590781598291326	31.0	31.0	34.0	30.0	34.0
2	31.83521099342796	33.0	31.0	34.0	30.0	34.0
3	31.989165869266603	33.0	31.0	34.0	30.0	34.0
4	35.7183071173782	37.0	35.0	37.0	35.0	37.0
5	35.37075538690582	37.0	35.0	37.0	33.0	37.0
6	35.43710638367875	37.0	35.0	37.0	33.0	37.0
7	35.28530380257014	37.0	35.0	37.0	32.0	37.0
8	35.4762712933235	37.0	35.0	37.0	33.0	37.0
9	36.99863322430201	39.0	37.0	39.0	33.0	39.0
10	36.475515933835446	38.0	35.0	39.0	32.0	39.0
11	36.67415354498022	39.0	35.0	39.0	32.0	39.0
12	36.37215369303616	38.0	35.0	39.0	32.0	39.0
13	36.20361667767947	38.0	35.0	39.0	32.0	39.0
14	37.328815799214205	39.0	36.0	41.0	32.0	41.0
15	37.30770960197627	39.0	36.0	40.0	32.0	41.0
16	37.116230769863485	39.0	35.0	40.0	32.0	41.0
17	37.03199790528258	39.0	35.0	40.0	32.0	41.0
18	36.78230292794336	38.0	35.0	40.0	32.0	41.0
19	36.5775278495971	38.0	35.0	40.0	31.0	41.0
20	36.590582819478676	38.0	35.0	40.0	31.0	41.0
21	36.706459900144495	38.0	35.0	40.0	31.0	41.0
22	36.62075470146164	38.0	35.0	40.0	31.0	41.0
23	36.46985690667266	38.0	35.0	40.0	31.0	41.0
24	36.25261496955257	38.0	35.0	40.0	31.0	41.0
25	36.37943448113246	38.0	35.0	40.0	31.0	41.0
26	36.26054967139806	38.0	35.0	40.0	31.0	41.0
27	36.138466579112595	38.0	35.0	40.0	30.0	41.0
28	36.150049214892235	38.0	35.0	40.0	30.0	41.0
29	35.8637241004916	38.0	35.0	40.0	30.0	41.0
30	36.02524079654098	38.0	35.0	40.0	30.0	41.0
31	35.765232626046334	38.0	35.0	40.0	30.0	41.0
32	35.62586400239083	38.0	35.0	40.0	29.0	41.0
33	35.647877727725124	38.0	35.0	40.0	30.0	41.0
34	35.40947612872093	38.0	35.0	40.0	28.0	41.0
35	35.32434807403894	38.0	35.0	40.0	28.0	41.0
36	35.22731385392362	38.0	35.0	40.0	27.0	41.0
37	35.24085686006246	38.0	34.0	40.0	27.0	41.0
38	35.107452971674704	37.0	34.0	40.0	27.0	41.0
39	34.9801920340419	37.0	34.0	40.0	26.0	41.0
40	34.72941953844931	37.0	34.0	40.0	24.0	41.0
41	34.84469617001209	37.0	34.0	40.0	25.0	41.0
42	34.86469880211775	37.0	34.0	40.0	25.0	41.0
43	34.77249010904046	37.0	34.0	40.0	25.0	41.0
44	34.76913143255092	37.0	34.0	40.0	25.0	41.0
45	34.57003320291616	37.0	34.0	40.0	24.0	41.0
46	33.566573080687746	35.0	33.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	3.0
11	7.0
12	9.0
13	15.0
14	40.0
15	49.0
16	84.0
17	149.0
18	295.0
19	542.0
20	1074.0
21	2113.0
22	3612.0
23	5082.0
24	6349.0
25	7378.0
26	8349.0
27	8870.0
28	10480.0
29	12808.0
30	16389.0
31	21343.0
32	28260.0
33	38956.0
34	61025.0
35	89588.0
36	77987.0
37	86350.0
38	129520.0
39	112717.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.292100667074276	40.58624669958626	16.399937487490643	23.721715145848812
2	20.12011723837281	43.47374885873544	15.199313459107772	21.20682044378398
3	16.67726820334113	37.43128422080076	24.592092167566427	21.29935540829168
4	22.221689098969915	37.69257554280325	15.944939639785375	24.140795718441467
5	16.068593770134925	45.42575679892084	16.71853194306975	21.78711748787449
6	17.525848100085817	41.68720714397344	23.19460857024569	17.592336185695054
7	67.28374921516641	22.183304224803756	6.3928637035371665	4.140082856492664
8	68.71536793272777	15.243730242071468	5.362366921012154	10.678534904188613
9	68.1572792801191	17.783849290016917	8.806998110915835	5.251873318948145
10	32.158299221061235	40.63011512720473	12.718279699610942	14.493305952123094
11	23.29852190816693	34.492099570363585	24.49393656077011	17.715441960699373
12	25.004455387180002	32.97877590636284	28.94301765430034	13.073751052156817
13	19.25618339196166	42.159341096217176	22.03524828159144	16.549227230229732
14	14.319614396521233	39.28472528768092	29.885092137406883	16.51056817839096
15	12.984094953211581	38.62491671853194	28.841023560087407	19.54996476816907
16	15.43935601148256	38.42202524079654	29.480680070299154	16.657938677421743
17	12.76434154861033	35.581818730173524	28.13762074099258	23.516218980223563
18	15.38630263183148	36.4674126127213	26.247165688309337	21.89911906713789
19	17.393420284212578	38.34758600268145	28.039190956523647	16.21980275658232
20	18.037463637186168	40.587617587949346	26.238803269294568	15.136115505569919
21	15.952616614618606	36.28741497064928	28.448264044065834	19.31170437066628
22	17.083462425320857	37.25005277920198	24.960038604216304	20.70644619126086
23	16.769254812503597	38.19185308463591	23.621777384180497	21.41711471868
24	12.969974803071887	40.53634636317026	29.326455129452988	17.167223704304863
25	14.811626230029585	36.530336388586534	28.503647933934147	20.154389447449734
26	19.999479062422033	40.181149188297	21.602321736531707	18.21705001274926
27	13.839118025262731	39.72039360946681	26.013840488913626	20.42664787635684
28	15.240303021163776	36.365555607344675	28.1006067551895	20.293534616302054
29	13.856528307473809	43.46511226204805	24.580165438807654	18.098193991670485
30	17.431256803033502	36.611081713171764	23.479067905584177	22.47859357821055
31	19.394506027796133	39.97921733241575	22.829540999158272	17.79673564062984
32	16.8813934806033	38.91444834081381	23.304553816964468	20.899604361618415
33	16.755545928872827	36.854414397617944	24.017552854600837	22.37248681890839
34	14.869888985460358	38.3127654382593	23.339648559059242	23.4776970172211
35	14.775434777244351	36.972859152187795	24.85818159883968	23.39352447172817
36	17.022183715491312	38.40543749160331	26.751789694758	17.820589098147384
37	14.820674093225891	37.86818634211342	23.360348973341704	23.950790591318988
38	16.408711173014336	38.516753626685166	27.244624061284195	17.829911139016303
39	15.582888023096727	36.557891244684384	25.19857317939171	22.660647552827186
40	17.45332810567904	37.35849004872137	22.789922325465348	22.398259520134236
41	14.810392430502814	33.067335294617614	29.340986546101604	22.781285728777963
42	18.777057909066233	33.30436189259364	23.659888080674037	24.25869211766609
43	17.570813238394745	34.40545942581712	26.500780035478588	21.522947300309546
44	16.36648781143156	31.857115047693206	27.00773455214448	24.76866258873075
45	21.08810151154151	35.413610728023976	24.982658262207075	18.51562949822744
46	15.007389088276984	35.443496094339054	31.771571613837196	17.77754320354676
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	50488.0
1	37534.0
2	24580.0
3	14880.0
4	5180.0
5	4172.0
6	3164.0
7	3164.0
8	2971.5
9	2779.0
10	3073.0
11	3367.0
12	3247.0
13	3127.0
14	3984.0
15	4841.0
16	4437.0
17	4033.0
18	4015.5
19	3998.0
20	3998.0
21	4497.5
22	4997.0
23	5838.5
24	6680.0
25	9889.5
26	13099.0
27	16160.0
28	19221.0
29	17410.5
30	15600.0
31	15600.0
32	19441.0
33	23282.0
34	24196.0
35	25110.0
36	30373.0
37	35636.0
38	32002.0
39	28368.0
40	39335.0
41	50302.0
42	46711.5
43	43121.0
44	43121.0
45	55770.0
46	68419.0
47	66893.0
48	65367.0
49	56885.5
50	48404.0
51	43644.5
52	38885.0
53	35888.5
54	32892.0
55	29920.5
56	26949.0
57	26949.0
58	23332.5
59	19716.0
60	17846.5
61	15977.0
62	14939.0
63	13901.0
64	11591.5
65	9282.0
66	7735.5
67	6189.0
68	5630.0
69	5071.0
70	5071.0
71	3762.5
72	2454.0
73	2254.5
74	2055.0
75	1603.5
76	1152.0
77	1074.5
78	997.0
79	737.5
80	478.0
81	478.0
82	323.0
83	168.0
84	107.5
85	47.0
86	31.5
87	16.0
88	30.5
89	45.0
90	26.0
91	7.0
92	6.0
93	5.0
94	5.0
95	3.5
96	2.0
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	729454.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.988774766631085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.09030742160878	14.448617095526467
2	10.09117357488243	3.8323804428884367
3	3.6519135707227424	2.080360927849729
4	1.8352251939276267	1.393947114141542
5	1.0911015391417433	1.0359340687143535
6	0.7241268247427949	0.8250168706510004
7	0.5370671531839192	0.7138773044455637
8	0.41442267464071436	0.6295503061549887
9	0.3288874382180276	0.5620652539106779
>10	3.5110433966340273	15.675295320309765
>50	1.1582718221181036	15.52699858389976
>100	0.4976439911164085	18.915530161855436
>500	0.04708422041130502	5.99605717662853
>1k	0.01593619767767247	4.23693680518647
>5k	0.0036218631085619245	5.098959431492467
>10k+	0.0021731178651371546	9.028473136344822
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43944	6.02423182270575	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10971	1.504001623131822	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTAT	10723	1.4700035917275112	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGT	9629	1.3200284048068829	No Hit
AATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGT	8763	1.2013094725644113	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7770	1.065180258110861	No Hit
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTT	5708	0.7825030776443751	RNA PCR Primer, Index 29 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5200	0.7128619488000614	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACTA	2753	0.37740556635510947	No Hit
AATGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCC	2623	0.3595840176351079	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGC	1886	0.25854954527632995	No Hit
AACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTC	1743	0.23894584168432825	No Hit
AGCACTGGGATTCTATATTTAATTAGGTGTTTTAATCAAGATATTT	1643	0.22523695805355787	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCACTAACCT	1567	0.21481820649417235	No Hit
ACACGGGTGTAAATTAAACCTTACGATGGGAAAGACAAAGTTTAGT	1516	0.20782667584247944	No Hit
AAAGCAGGGGATAGAGTTGTTCTGTTAATTTTACTTTTAGTGTTTT	1423	0.19507741406586296	No Hit
GTTAGAGGGGTAAGTAAATAGTTTTATTTCTTTTTGTATTAAACTT	1309	0.1794492867267847	No Hit
TAACATGGGAGGAATATTCAGGTTATTTAACATTGTTTTTAAATGG	1243	0.17040142353047621	No Hit
GCATGGGGTTTATAAAATAGATTCTCCTTACTGATAGGTTTGTGTG	1241	0.1701272458578608	No Hit
TTCCTTGGGAATGTTCACTTAAAAAGACAGATTTTGGAGATTGTAT	1227	0.16820800214955295	No Hit
AAACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGT	1221	0.1673854691317067	No Hit
TAGCGCGGGATTTTTGGTCCTTGGTTTAATTATCTTCAAAATACAT	1134	0.15545874037293647	No Hit
AAGCAGGAGACTCCTTAATACGTATACATCATGCATTGCTATTGAT	1075	0.14737049903078192	No Hit
GGCTATGGGACAATAACACTTATTTCTTAGAAAGAAACACAGGTTT	1050	0.14394327812308932	No Hit
AGCAAGGGAGATCTTTCCATCTTCTGAGATCTTCTTTAATTTCTTT	1048	0.14366910045047393	No Hit
AGACCAGGGGTGGATTTAATGAATTTTTAGGTGAAAGGATGTTTTA	1042	0.1428465674326277	No Hit
GCTCTTGGGCATTTTATATTATGGTACTTTAATCACTGTTTTTTCT	1017	0.1394193465249351	No Hit
GCAAGTGCTTTGGGGTTTTTTATTAATATTAATTAGAACATTGATA	1017	0.1394193465249351	No Hit
GGGAGCGGGTCTTAAAATATAACTAAACTAATTTTAAAATTGCTTA	1017	0.1394193465249351	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1008	0.13818554699816576	No Hit
CGTTAAGGGATTTATTTGTTTATATAAGCATACAGTTTAAAATCAG	998	0.1368146586350887	No Hit
CTGAATGGGAATGTAATTCTGCCACCTAAATACTATATGTTTTGGT	967	0.13256490470954987	No Hit
CTGGAAGGGGTGATGATTTTTAAAACTGTCTTTTAAATTTATGTAA	957	0.13119401634647285	No Hit
GGCTGGGGGATACTATGCCACCTACTCTTTCTGATCCTTAAGAAAA	920	0.12612172940308777	No Hit
GGTGGGGGGGATGAGACCACAAGATAGGAAAATGTACATTACTTCT	886	0.12146070896862586	No Hit
ACGGGTATCACTAAATTGAGTGATACAAATTTCATCTATTTTATTA	882	0.12091235362339503	No Hit
GAACAGGGTCTGTAAATGTCTGCACTAAGGGTTTCTTTCAGTTTGC	875	0.1199527317692411	No Hit
CAAAAAGGGAAGTTAGGATTTGTTGCCATTGATTCCTTTTTATAAC	869	0.11913019875139487	No Hit
CAAGCTGGGATCTTCCATTTTATTTAATTTTGTTCCTTTTTCTTTT	860	0.11789639922462553	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	849	0.11638842202524079	No Hit
TAAACAGTTCTTTTCTCATACATTCTGTTTTTTATCAATATTCCCT	817	0.11200157926339427	No Hit
GAAGGAGGGAAAGGAAAGGGATAGTGATAGAATCATACTTTAAGAA	803	0.11008233555508641	No Hit
GGCAACGGGAGCTATACAAACCCATATATAATTATACATAATATAT	799	0.10953398020985558	No Hit
AGCAATGGGCAGAATATGGAAACAAAACTAAACAGAGACACAGTGA	792	0.10857435835570166	No Hit
TTTTTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAA	785	0.10761473650154774	No Hit
AGGCGTGGGATTTCCATTTTTGTAAGTATTTTTCTGTGCATTGCAA	768	0.10528422628431676	No Hit
ATCCACGGGATTTTTAGACCTCCCGTTTTTCTAATCTGGGCTATTT	758	0.10391333792123972	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	745	0.10213118304923957	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3708883630770413E-4	0.0	0.0	0.8813441286222298	0.0
2	1.3708883630770413E-4	0.0	0.0	1.0828647179945547	0.0
3	1.3708883630770413E-4	0.0	0.0	1.5577404469644418	0.0
4	1.3708883630770413E-4	0.0	0.0	4.003268197857576	0.0
5	1.3708883630770413E-4	0.0	0.0	4.26455951986006	0.0
6	1.3708883630770413E-4	0.0	0.0	5.441741357234315	0.0
7	1.3708883630770413E-4	0.0	0.0	6.296901518121773	0.0
8	1.3708883630770413E-4	0.0	0.0	6.627696880132263	0.0
9	1.3708883630770413E-4	0.0	0.0	8.72611569749429	0.0
10	1.3708883630770413E-4	0.0	0.0	9.557696578536824	0.0
11	1.3708883630770413E-4	0.0	0.0	11.967307054317338	0.0
12	1.3708883630770413E-4	0.0	0.0	12.476180814691537	0.0
13	1.3708883630770413E-4	0.0	0.0	12.719102232628789	0.0
14	1.3708883630770413E-4	0.0	0.0	13.144900158200517	0.0
15	1.3708883630770413E-4	0.0	0.0	13.377677002250998	0.0
16	1.3708883630770413E-4	0.0	0.0	13.771670317799341	0.0
17	1.3708883630770413E-4	0.0	0.0	14.212410926528609	0.0
18	1.3708883630770413E-4	0.0	0.0	15.276494473949008	0.0
19	1.3708883630770413E-4	0.0	0.0	15.584944355641342	0.0
20	1.3708883630770413E-4	0.0	0.0	15.832938060521979	0.0
21	1.3708883630770413E-4	0.0	0.0	16.155233914681393	0.0
22	1.3708883630770413E-4	0.0	0.0	16.511664889081423	0.0
23	1.3708883630770413E-4	0.0	0.0	16.858636733776223	0.0
24	1.3708883630770413E-4	0.0	0.0	17.10292904007655	0.0
25	2.7417767261540826E-4	0.0	0.0	17.32377915536826	0.0
26	2.7417767261540826E-4	0.0	0.0	17.52036454663351	0.0
27	2.7417767261540826E-4	0.0	0.0	17.746149860032297	0.0
28	2.7417767261540826E-4	0.0	0.0	17.969056307868627	0.0
29	2.7417767261540826E-4	0.0	0.0	18.194841621267415	0.0
30	2.7417767261540826E-4	0.0	0.0	18.503428591796055	0.0
31	2.7417767261540826E-4	0.0	0.0	18.73291530377515	0.0
32	2.7417767261540826E-4	0.0	0.0	18.955684662775173	0.0
33	2.7417767261540826E-4	0.0	0.0	19.1821554203555	0.0
34	2.7417767261540826E-4	0.0	0.0	19.395602738486595	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGC	20	0.0012554673	40.0	22
ACGCGAT	30	4.8650654E-6	40.0	21
AACGTTA	20	0.0012554673	40.0	21
CGGGTTA	20	0.0012554673	40.0	6
ACTATTA	20	0.0012554673	40.0	15
AAGTACG	30	4.8650654E-6	40.0	1
AAGTAAC	55	5.456968E-12	40.0	18
AACCGAC	25	7.7918514E-5	40.0	34
TCGTTGA	50	7.8216544E-11	40.0	24
AGCCGAC	25	7.7918514E-5	40.0	36
TCGTTAA	30	4.8650654E-6	40.0	18
CCGGGTC	30	4.8650654E-6	40.0	5
CGAACTA	20	0.0012554673	40.0	33
CTCAACG	25	7.7918514E-5	40.0	1
CAAGTCG	30	4.8650654E-6	40.0	1
AGTCAAT	85	0.0	40.0	12
TTTACGG	25	7.7918514E-5	40.0	2
CGACGCA	25	7.7918514E-5	40.0	37
GCGGTAC	50	7.8216544E-11	40.0	24
CCTAGAT	20	0.0012554673	40.0	38
>>END_MODULE