FastQCFastQC Report
Sat 18 Jun 2016
SRR3548328_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548328_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857091
Sequences flagged as poor quality0
Sequence length46
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA561116.546679407437484No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA136391.591312941099603No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA116121.3548152996589629No Hit
GCATACGGGATTTGCTGAAGGTAATCCAATAAGATCTTTTTTTAAA67370.7860308882020696No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61620.718943496081513No Hit
CTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTT42880.5002969346312118No Hit
GACATAGGGATCCTCAATTATAGCACTTGGTTTTCTTTCTATATTT19170.22366353164366445No Hit
AATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGT18950.22109670968426923No Hit
GGGATGGGGATTTTTTTCTAAGGTGAAGAAAATGTTCAAAATTTAA18430.21502967596206238No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGT18360.21421295988407302No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTAT18080.21094609557211547No Hit
GGCGAAGGGGAGCATTTTTTAAAATATGAACTTCTAAAATTAAATT16880.19694524852086884No Hit
CGACCCGGGATTTTCAGTATTTCTTTGAATAAAGCTTAATGTTTCT15600.1820110116662058No Hit
ATGTTAGGGTCCTAATATTGTGAACTGGTGAAAGAGGGAATGATGC15260.17804410500168594No Hit
ATGTAAGGGGAACTTCAATTATGATTTTTATCCAGACAATTTCTAT14530.16952692304551092No Hit
TATTAAGGGATGTTTCATATGATCAAGCTGTATTTTTAAATTCAAA14130.16485997402842872No Hit
ATGTAAGGGATATTTTGAGGTGAAACTTAGTGAATTTTTTTCTGAT13940.1626431732453147No Hit
AAGCTGGGAACTTGGGGAGTTTTTAGCTTTTAAAAGTTGCATCTAT13610.15879294030622187No Hit
AAACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGT12850.1499257371737657No Hit
ATCTATGGGAATCTCATTCCTTTTGATTGTTAATGTTTTTGTTCAG12590.14689222031266225No Hit
TCTAAAGGGAACTAACTCAGGTTCCCCTCATTAAATTATACTTTCA12050.14059183913960127No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11790.13755832227849785No Hit
GGAGAAGGGGAAGAAGGAGAAGGAGAAGGAGAAGGAGAAGAAGAAG11560.13487482659367558No Hit
GAATCTGGGTATTTGTTTTTCTTCTTGACAAGATTTTCATTTAATT11040.12880779287146873No Hit
ATTAAAGGGGTTCTTGTAACCTTTACTTCATGAAGGACTACTTTTT10870.1268243395392088No Hit
CTATAGCGGGGTTAATGTGTTCAAGGTAATAAGTTCTCTAACATTT10720.12507423365780296No Hit
ACACAAGATAGGAAAATGTACATTACTTCTAGTTTTAGACTTTAGA10710.12495755993237592No Hit
TGTATTGGGATTTGTAAAACAAAGTCTACTCTAAAATTATTATTTG10690.1247242124815218No Hit
GCTTTGGGGTCAATATTTTCACAACCTCAATCTTATTAATCTTCAT10680.12460753875609475No Hit
CCTAATGGGGATTATATTACTAATTTTCTTTACCCACATTTGAAAT10540.12297410660011597No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10130.11819048385760672No Hit
ATATCTGGGAAGATTTCTCATAACCATCTAAAGTCAAAACTGTTGC9900.11550698817278444No Hit
TCGCATGGGTAGTTCATAAAAGCAAAAGTTATACATTAGGGTTATT9840.11480694582022212No Hit
CAATCCGGGAATATTTAACCATCTACCTCTGTTTTTAATTTTCTTT9840.11480694582022212No Hit
CTTCAAGGGAGGTAATTCATATTTTGTTGTAAATCTATAGCAGTTT9780.1141069034676598No Hit
CTTTAAGGGGTTTATATAAATAATGTTGCTAATTTCCTTCTTATAT9650.11259014503710808No Hit
GCCATGGGGGAATTATACAAACACACTACAGACTGATTTTTTTTTA9340.10897325954886937No Hit
AAACTAGGGATTTTCAATTGAGTTTAAGTGTCTAGAACTAAAGTAG9280.10827321719630704No Hit
GCTTCAGGGTGGGATTACTTGAAAAAAATCTGTATTGCAATTAAGG9260.10803986974545292No Hit
ACACCTGGGAATCTTGTCTATGGCTTTGTAATATTCAGCAAGGTAT9220.1075731748437447No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9210.10745650111831766No Hit
GGTATTGGGATAAACTTCAACATTAATATATCTGCATAAAATGGAG9200.1073398273928906No Hit
CTTCCCGGGAGAGGACTTTTGGTTTTTGTTTTGTTTTTTGGTGTTA9130.1065231113149012No Hit
AGCCAAGGGCAATGTACCTATATATGCATAATTTTTGTTGGGTGCT9130.1065231113149012No Hit
TCCGGGGGGGAAATTACAAAAGAGGTTTGTCTCCCTCATCAAAAAC9030.10535637406063067No Hit
GCAGTATGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTT8910.10395628935550599No Hit
GCGTGGGAGATATTTATATTTTTATTTTATTTTTTTCTACCCTGAG8850.10325624700294367No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG304.866062E-640.0000041
TTAGGTA304.866062E-640.00000417
CGGGTCC200.001255589840.0000046
GAGTGCG800.040.0000049
CTACTAC200.001255589840.00000424
GGTCGAT200.001255589840.0000048
CCTAGTA200.001255589840.00000428
CGAAACG304.866062E-640.0000041
GCGAATT200.001255589840.00000419
CGGCCTA200.001255589840.00000414
TCATTCG200.001255589840.0000041
CTATCCG200.001255589840.0000041
GGGTCGA200.001255589840.0000047
CCGGCAC200.001255589840.00000433
GTCCTGA200.001255589840.00000416
ATCAGTC200.001255589840.00000416
TAGGTAT304.866062E-640.00000418
TGGCGCG200.001255589840.0000041
GGCCGAT401.9274012E-840.0000048
CACGATT200.001255589840.00000436
Produced by FastQC (version 0.11.5)