##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548328_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	857091
Sequences flagged as poor quality	0
Sequence length	46
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.725763075332726	33.0	31.0	34.0	30.0	34.0
2	31.96824257867601	33.0	31.0	34.0	30.0	34.0
3	32.14421572505137	34.0	31.0	34.0	30.0	34.0
4	35.86600372655879	37.0	35.0	37.0	35.0	37.0
5	35.5533916468613	37.0	35.0	37.0	33.0	37.0
6	35.627646305934846	37.0	35.0	37.0	33.0	37.0
7	35.54025651885272	37.0	35.0	37.0	33.0	37.0
8	35.83938811631437	37.0	35.0	37.0	35.0	37.0
9	37.32553369478853	39.0	37.0	39.0	34.0	39.0
10	36.734377096481005	39.0	37.0	39.0	32.0	39.0
11	36.91912760722024	39.0	37.0	39.0	33.0	39.0
12	36.69454001967119	39.0	35.0	39.0	32.0	39.0
13	36.4916735795849	39.0	35.0	39.0	32.0	39.0
14	37.71954786597922	40.0	37.0	41.0	33.0	41.0
15	37.62313103276082	39.0	36.0	41.0	33.0	41.0
16	37.58661799038842	39.0	36.0	41.0	32.0	41.0
17	37.44778325755375	39.0	36.0	40.0	32.0	41.0
18	37.16657157758044	38.0	36.0	40.0	32.0	41.0
19	37.10349659487732	39.0	35.0	40.0	32.0	41.0
20	36.94095376103588	39.0	35.0	40.0	32.0	41.0
21	36.98966154118991	39.0	35.0	40.0	32.0	41.0
22	36.996643296919466	39.0	35.0	40.0	32.0	41.0
23	36.84733826396497	39.0	35.0	40.0	32.0	41.0
24	36.51844669935864	38.0	35.0	40.0	31.0	41.0
25	36.68421322823364	38.0	35.0	40.0	32.0	41.0
26	36.68266146768546	38.0	35.0	40.0	32.0	41.0
27	36.53577158084731	38.0	35.0	40.0	31.0	41.0
28	36.56713931192837	38.0	35.0	40.0	31.0	41.0
29	36.29789485597212	38.0	35.0	40.0	31.0	41.0
30	36.308425826429165	38.0	35.0	40.0	31.0	41.0
31	36.260742441584384	38.0	35.0	40.0	31.0	41.0
32	36.11534481169444	38.0	35.0	40.0	31.0	41.0
33	36.10293422752076	38.0	35.0	40.0	31.0	41.0
34	35.971595781544785	38.0	35.0	40.0	30.0	41.0
35	35.82257076553132	38.0	35.0	40.0	30.0	41.0
36	35.771349833331584	38.0	35.0	40.0	30.0	41.0
37	35.64986798367968	38.0	35.0	40.0	29.0	41.0
38	35.59019520681001	38.0	35.0	40.0	29.0	41.0
39	35.44279078884273	38.0	35.0	40.0	28.0	41.0
40	35.331137533820794	38.0	35.0	40.0	27.0	41.0
41	35.38503379454457	38.0	35.0	40.0	28.0	41.0
42	35.27263499441716	38.0	35.0	40.0	27.0	41.0
43	35.15564858340596	38.0	34.0	40.0	27.0	41.0
44	35.045066393183454	37.0	34.0	40.0	26.0	41.0
45	34.85594761816423	37.0	34.0	40.0	25.0	41.0
46	34.108937090693985	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	2.0
9	14.0
10	15.0
11	36.0
12	29.0
13	45.0
14	45.0
15	72.0
16	124.0
17	183.0
18	342.0
19	601.0
20	1136.0
21	1768.0
22	2683.0
23	3907.0
24	5234.0
25	6694.0
26	8387.0
27	9393.0
28	11026.0
29	13545.0
30	17094.0
31	21852.0
32	29498.0
33	41350.0
34	67098.0
35	104207.0
36	90979.0
37	101036.0
38	158455.0
39	160240.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.467732131127267	35.50521473215797	17.611082137135963	25.415970999578807
2	20.480205719112675	38.26058143184329	18.93964584857384	22.319567000470194
3	17.536177605411794	40.09562578536001	20.157836215757722	22.21036039347047
4	18.0347244341616	39.00507647379333	20.691151814684787	22.26904727736028
5	14.652469807756702	43.50704884312167	16.5003482710704	25.340133078051224
6	15.806256278504849	46.26964931378348	18.899276739576077	19.024817668135587
7	74.09866630264465	17.871614566014575	4.518540038338986	3.5111790930017936
8	75.08292585034728	17.24239316478647	3.8736843579036533	3.800996626962598
9	71.65796864043608	18.806987822763276	6.457774028662068	3.077269508138576
10	31.423384448092445	44.4537394512368	11.814148089292734	12.30872801137802
11	23.959416211347452	37.21926843240683	24.673342737235604	14.147972619010115
12	24.744047014844398	34.689082022795716	27.921189231948535	12.645681730411356
13	18.520787174290714	38.901936900515814	25.993155919266446	16.584120005927026
14	15.437683979880784	41.683321840971374	28.261643162744683	14.61735101640316
15	12.93398250594161	39.5966122617085	30.32980161966466	17.139603612685235
16	16.507348694596022	36.913933292964224	30.14464041741192	16.434077595027833
17	16.077756037573607	37.842189452461874	26.480152049198974	19.59990246076554
18	15.121731531424318	39.590195206810016	28.021528635815802	17.26654462594987
19	16.930524296719952	40.862638856317474	25.84171342366213	16.365123423300442
20	19.27764963113602	39.893313545469496	25.981721894174598	14.847314929219884
21	18.405280186117924	39.82762623805407	26.293357414790265	15.473736161037744
22	18.460116837068643	36.63146620370533	26.652829162831015	18.255587796395016
23	16.199446733194026	40.26398597115126	24.33195541663604	19.204611879018678
24	14.489476613335107	40.276586733497375	28.95211826982199	16.281818383345524
25	15.811506596149066	39.40235050887245	27.13924192413641	17.64690097084207
26	17.610148747332545	40.680044476024136	23.469736585730104	18.240070190913215
27	14.818379845313975	39.93134917995872	26.500569951148712	18.749701023578595
28	15.094429879674387	39.76030549848266	27.662756930127607	17.482507691715348
29	16.03365336936218	38.61841974772807	27.068537646527613	18.279389236382134
30	16.930640970445378	39.6647497173579	26.797271234909715	16.60733807728701
31	19.62183712114583	40.64515903212144	23.780438716542353	15.952565130190377
32	18.191650594860988	40.16866353747735	24.81323453402264	16.82645133363902
33	18.396763004161752	40.70011235679758	23.58326012057063	17.319864518470034
34	16.130842582642916	38.76659537902043	26.50360346800981	18.598958570326836
35	16.53290024046455	38.44422587566548	27.20026228253476	17.822611601335215
36	19.03146807048493	38.82236541977456	26.20153519287917	15.944631316861338
37	16.63860663570146	39.50467336607198	27.186378109208942	16.67034188901762
38	17.005895523345828	38.50664631876895	28.620998237060007	15.86645992082521
39	16.633122970606387	38.82084866134401	27.484129456498785	17.061898911550816
40	18.082210640410413	36.68875300289001	26.405947559827368	18.823088796872213
41	15.916046254131707	35.28050113698546	29.64621026238754	19.157242346495295
42	17.50467569954649	35.153560123720816	27.95152440055957	19.390239776173125
43	17.212408017351716	35.97319304484588	28.733938403273395	18.080460534529006
44	18.121296338428476	35.162193979402424	27.35077138833566	19.36573829383344
45	18.97966493639532	39.233406954454075	25.157072002856175	16.62985610629443
46	17.38706858431602	36.040980479319	29.08804315994451	17.483907776420473
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	64025.0
1	48419.0
2	32813.0
3	19783.0
4	6753.0
5	5538.5
6	4324.0
7	4324.0
8	4170.5
9	4017.0
10	4156.0
11	4295.0
12	4300.5
13	4306.0
14	5761.5
15	7217.0
16	6688.5
17	6160.0
18	6187.5
19	6215.0
20	6215.0
21	7419.5
22	8624.0
23	9498.0
24	10372.0
25	9860.5
26	9349.0
27	18624.5
28	27900.0
29	27708.0
30	27516.0
31	27516.0
32	31501.0
33	35486.0
34	37980.0
35	40474.0
36	39341.0
37	38208.0
38	45532.5
39	52857.0
40	51499.0
41	50141.0
42	47838.0
43	45535.0
44	45535.0
45	50938.5
46	56342.0
47	58064.5
48	59787.0
49	58128.0
50	56469.0
51	50893.5
52	45318.0
53	40093.5
54	34869.0
55	32260.0
56	29651.0
57	29651.0
58	26044.5
59	22438.0
60	20070.5
61	17703.0
62	16120.0
63	14537.0
64	12531.0
65	10525.0
66	9226.5
67	7928.0
68	6823.5
69	5719.0
70	5719.0
71	4677.5
72	3636.0
73	3046.0
74	2456.0
75	1901.0
76	1346.0
77	1123.5
78	901.0
79	670.5
80	440.0
81	440.0
82	363.0
83	286.0
84	180.0
85	74.0
86	66.0
87	58.0
88	33.0
89	8.0
90	7.5
91	7.0
92	6.5
93	6.0
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	857091.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.01061786620322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.29637732290718	13.561342786723307
2	10.511066321445648	3.7862159776375184
3	3.8624139990074595	2.0869338773159147
4	1.9328603802148465	1.3924803878709544
5	1.0692380144585754	0.9628818643215636
6	0.7219652194187238	0.7801823807784116
7	0.563287025960026	0.7101603161489283
8	0.40859209457396245	0.5887196862818562
9	0.35230512165943895	0.5710709626662951
>10	3.3891705184516825	14.353966475097529
>50	1.198898753323349	15.33061251151097
>100	0.5942424331076099	20.524984474880952
>500	0.07940588919948348	9.775303731657136
>1k	0.016922566550709596	4.525366957954791
>5k	0.0013017358885161225	1.5120896381374687
>10k+	0.001952603832774184	9.537687971016414
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	56111	6.546679407437484	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13639	1.591312941099603	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11612	1.3548152996589629	No Hit
GCATACGGGATTTGCTGAAGGTAATCCAATAAGATCTTTTTTTAAA	6737	0.7860308882020696	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6162	0.718943496081513	No Hit
CTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTT	4288	0.5002969346312118	No Hit
GACATAGGGATCCTCAATTATAGCACTTGGTTTTCTTTCTATATTT	1917	0.22366353164366445	No Hit
AATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGT	1895	0.22109670968426923	No Hit
GGGATGGGGATTTTTTTCTAAGGTGAAGAAAATGTTCAAAATTTAA	1843	0.21502967596206238	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGT	1836	0.21421295988407302	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTAT	1808	0.21094609557211547	No Hit
GGCGAAGGGGAGCATTTTTTAAAATATGAACTTCTAAAATTAAATT	1688	0.19694524852086884	No Hit
CGACCCGGGATTTTCAGTATTTCTTTGAATAAAGCTTAATGTTTCT	1560	0.1820110116662058	No Hit
ATGTTAGGGTCCTAATATTGTGAACTGGTGAAAGAGGGAATGATGC	1526	0.17804410500168594	No Hit
ATGTAAGGGGAACTTCAATTATGATTTTTATCCAGACAATTTCTAT	1453	0.16952692304551092	No Hit
TATTAAGGGATGTTTCATATGATCAAGCTGTATTTTTAAATTCAAA	1413	0.16485997402842872	No Hit
ATGTAAGGGATATTTTGAGGTGAAACTTAGTGAATTTTTTTCTGAT	1394	0.1626431732453147	No Hit
AAGCTGGGAACTTGGGGAGTTTTTAGCTTTTAAAAGTTGCATCTAT	1361	0.15879294030622187	No Hit
AAACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGT	1285	0.1499257371737657	No Hit
ATCTATGGGAATCTCATTCCTTTTGATTGTTAATGTTTTTGTTCAG	1259	0.14689222031266225	No Hit
TCTAAAGGGAACTAACTCAGGTTCCCCTCATTAAATTATACTTTCA	1205	0.14059183913960127	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1179	0.13755832227849785	No Hit
GGAGAAGGGGAAGAAGGAGAAGGAGAAGGAGAAGGAGAAGAAGAAG	1156	0.13487482659367558	No Hit
GAATCTGGGTATTTGTTTTTCTTCTTGACAAGATTTTCATTTAATT	1104	0.12880779287146873	No Hit
ATTAAAGGGGTTCTTGTAACCTTTACTTCATGAAGGACTACTTTTT	1087	0.1268243395392088	No Hit
CTATAGCGGGGTTAATGTGTTCAAGGTAATAAGTTCTCTAACATTT	1072	0.12507423365780296	No Hit
ACACAAGATAGGAAAATGTACATTACTTCTAGTTTTAGACTTTAGA	1071	0.12495755993237592	No Hit
TGTATTGGGATTTGTAAAACAAAGTCTACTCTAAAATTATTATTTG	1069	0.1247242124815218	No Hit
GCTTTGGGGTCAATATTTTCACAACCTCAATCTTATTAATCTTCAT	1068	0.12460753875609475	No Hit
CCTAATGGGGATTATATTACTAATTTTCTTTACCCACATTTGAAAT	1054	0.12297410660011597	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1013	0.11819048385760672	No Hit
ATATCTGGGAAGATTTCTCATAACCATCTAAAGTCAAAACTGTTGC	990	0.11550698817278444	No Hit
TCGCATGGGTAGTTCATAAAAGCAAAAGTTATACATTAGGGTTATT	984	0.11480694582022212	No Hit
CAATCCGGGAATATTTAACCATCTACCTCTGTTTTTAATTTTCTTT	984	0.11480694582022212	No Hit
CTTCAAGGGAGGTAATTCATATTTTGTTGTAAATCTATAGCAGTTT	978	0.1141069034676598	No Hit
CTTTAAGGGGTTTATATAAATAATGTTGCTAATTTCCTTCTTATAT	965	0.11259014503710808	No Hit
GCCATGGGGGAATTATACAAACACACTACAGACTGATTTTTTTTTA	934	0.10897325954886937	No Hit
AAACTAGGGATTTTCAATTGAGTTTAAGTGTCTAGAACTAAAGTAG	928	0.10827321719630704	No Hit
GCTTCAGGGTGGGATTACTTGAAAAAAATCTGTATTGCAATTAAGG	926	0.10803986974545292	No Hit
ACACCTGGGAATCTTGTCTATGGCTTTGTAATATTCAGCAAGGTAT	922	0.1075731748437447	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	921	0.10745650111831766	No Hit
GGTATTGGGATAAACTTCAACATTAATATATCTGCATAAAATGGAG	920	0.1073398273928906	No Hit
CTTCCCGGGAGAGGACTTTTGGTTTTTGTTTTGTTTTTTGGTGTTA	913	0.1065231113149012	No Hit
AGCCAAGGGCAATGTACCTATATATGCATAATTTTTGTTGGGTGCT	913	0.1065231113149012	No Hit
TCCGGGGGGGAAATTACAAAAGAGGTTTGTCTCCCTCATCAAAAAC	903	0.10535637406063067	No Hit
GCAGTATGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTT	891	0.10395628935550599	No Hit
GCGTGGGAGATATTTATATTTTTATTTTATTTTTTTCTACCCTGAG	885	0.10325624700294367	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.50021176281165E-4	0.0	0.0	0.5691344326331743	0.0
2	3.50021176281165E-4	0.0	0.0	0.7433283046957674	0.0
3	3.50021176281165E-4	0.0	0.0	1.0057275131812142	0.0
4	3.50021176281165E-4	0.0	0.0	2.21026705449013	0.0
5	3.50021176281165E-4	0.0	0.0	2.3636930034267074	0.0
6	3.50021176281165E-4	0.0	0.0	3.1721252469107712	0.0
7	3.50021176281165E-4	0.0	0.0	3.7720615430566884	0.0
8	3.50021176281165E-4	0.0	0.0	4.1195158973784585	0.0
9	3.50021176281165E-4	0.0	0.0	4.905663459305955	0.0
10	3.50021176281165E-4	0.0	0.0	5.776982840795202	0.0
11	3.50021176281165E-4	0.0	0.0	6.942553357811481	0.0
12	3.50021176281165E-4	0.0	0.0	7.3975808869769955	0.0
13	3.50021176281165E-4	0.0	0.0	7.579475224917775	0.0
14	3.50021176281165E-4	0.0	0.0	7.781787464808287	0.0
15	3.50021176281165E-4	0.0	0.0	7.937896909429687	0.0
16	3.50021176281165E-4	0.0	0.0	8.283484484144624	0.0
17	3.50021176281165E-4	0.0	0.0	8.68215860392887	0.0
18	3.50021176281165E-4	0.0	0.0	9.236358799707382	0.0
19	3.50021176281165E-4	0.0	0.0	9.49117421604007	0.0
20	3.50021176281165E-4	0.0	0.0	9.711104188470069	0.0
21	3.50021176281165E-4	0.0	0.0	10.021339624380609	0.0
22	3.50021176281165E-4	0.0	0.0	10.345575907342393	0.0
23	3.50021176281165E-4	0.0	0.0	10.68089619421975	0.0
24	3.50021176281165E-4	0.0	0.0	10.914360318799288	0.0
25	3.50021176281165E-4	0.0	0.0	11.123556308489997	0.0
26	3.50021176281165E-4	0.0	0.0	11.325985222105938	0.0
27	3.50021176281165E-4	0.0	0.0	11.532381042386397	0.0
28	3.50021176281165E-4	0.0	0.0	11.746127307368763	0.0
29	3.50021176281165E-4	0.0	0.0	11.971890966070115	0.0
30	3.50021176281165E-4	0.0	0.0	12.233823479653852	0.0
31	3.50021176281165E-4	0.0	0.0	12.472304574426753	0.0
32	3.50021176281165E-4	0.0	0.0	12.686167513134544	0.0
33	3.50021176281165E-4	0.0	0.0	12.898630367137212	0.0
34	3.50021176281165E-4	0.0	0.0	13.110159831336462	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	30	4.866062E-6	40.000004	1
TTAGGTA	30	4.866062E-6	40.000004	17
CGGGTCC	20	0.0012555898	40.000004	6
GAGTGCG	80	0.0	40.000004	9
CTACTAC	20	0.0012555898	40.000004	24
GGTCGAT	20	0.0012555898	40.000004	8
CCTAGTA	20	0.0012555898	40.000004	28
CGAAACG	30	4.866062E-6	40.000004	1
GCGAATT	20	0.0012555898	40.000004	19
CGGCCTA	20	0.0012555898	40.000004	14
TCATTCG	20	0.0012555898	40.000004	1
CTATCCG	20	0.0012555898	40.000004	1
GGGTCGA	20	0.0012555898	40.000004	7
CCGGCAC	20	0.0012555898	40.000004	33
GTCCTGA	20	0.0012555898	40.000004	16
ATCAGTC	20	0.0012555898	40.000004	16
TAGGTAT	30	4.866062E-6	40.000004	18
TGGCGCG	20	0.0012555898	40.000004	1
GGCCGAT	40	1.9274012E-8	40.000004	8
CACGATT	20	0.0012555898	40.000004	36
>>END_MODULE