FastQCFastQC Report
Sat 18 Jun 2016
SRR3548327_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548327_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2187276
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC179390.8201525550502086RNA PCR Primer, Index 4 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC157370.7194793889751454RNA PCR Primer, Index 48 (96% over 25bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC113090.5170358016089419No Hit
CTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGCT105130.4806435036090553TruSeq Adapter, Index 4 (96% over 27bp)
TCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC67790.3099288795744113RNA PCR Primer, Index 48 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC43990.20111773731344376TruSeq Adapter, Index 4 (96% over 26bp)
GCCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTG31470.14387759020809443RNA PCR Primer, Index 48 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTG30200.13807128135635374TruSeq Adapter, Index 4 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTG29050.13281360011265153TruSeq Adapter, Index 4 (96% over 25bp)
AGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTG25540.11676624257752564TruSeq Adapter, Index 4 (96% over 25bp)
GGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTG23620.10798820084890978RNA PCR Primer, Index 4 (96% over 25bp)
TGGCAAGGGGATGAACATTGTTAAAGTATTAATGCAAATGCCATTATAAAA23020.10524506280871733No Hit
GCTGTCTCTTATACACATCTGAAGCGAGCAATCTCGTATGCCGTCTTCTGC22730.10391921275595764Illumina PCR Primer Index 4 (96% over 26bp)
CCTGTCTCTTATACACATCTGAAGCGAGCAATCTCGTATGCCGTCTTCTGC21910.10017025743436128Illumina PCR Primer Index 4 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGC302.1664036E-645.00000437
TCCGCTA302.1664036E-645.00000436
GCGGTGC302.1664036E-645.0000041
CGACACA600.045.00000442
CGTTATA302.1664036E-645.00000419
AACGGCG302.1664036E-645.0000043
CTACGCG551.8189894E-1245.0000041
ACTACGT302.1664036E-645.00000440
GTCCGCT302.1664036E-645.00000435
TTACGAG302.1664036E-645.0000041
ACCCGTG302.1664036E-645.00000413
TATCGAT302.1664036E-645.00000413
GCCGTAA600.045.00000424
ATCGCAC302.1664036E-645.00000437
TAGTCGT302.1664036E-645.00000420
TCGAACG650.045.0000041
TACCGGT302.1664036E-645.00000440
TATCCGA302.1664036E-645.00000422
TCACGGT351.2125929E-745.032
TCGCCTC253.8919665E-545.040
Produced by FastQC (version 0.11.5)