##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548326_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4512881
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27019347507723	33.0	31.0	34.0	30.0	34.0
2	32.41018830321473	34.0	31.0	34.0	31.0	34.0
3	32.41698174625034	34.0	31.0	34.0	30.0	34.0
4	36.00488335500094	37.0	35.0	37.0	35.0	37.0
5	35.966490585504026	37.0	35.0	37.0	35.0	37.0
6	35.9470050284951	37.0	35.0	37.0	35.0	37.0
7	36.11215629217788	37.0	35.0	37.0	35.0	37.0
8	36.02342118039452	37.0	35.0	37.0	35.0	37.0
9	37.824985192385974	39.0	38.0	39.0	35.0	39.0
10	37.59834150291133	39.0	37.0	39.0	35.0	39.0
11	37.49045919890199	39.0	37.0	39.0	35.0	39.0
12	37.30979434201788	39.0	37.0	39.0	35.0	39.0
13	37.14697706409719	39.0	37.0	39.0	34.0	39.0
14	38.42500433758391	40.0	38.0	41.0	34.0	41.0
15	38.294632408875835	40.0	37.0	41.0	34.0	41.0
16	38.22880527982014	40.0	37.0	41.0	34.0	41.0
17	38.21208846410973	40.0	37.0	41.0	34.0	41.0
18	38.28333984432561	40.0	37.0	41.0	34.0	41.0
19	38.353280531881964	40.0	37.0	41.0	34.0	41.0
20	38.38826461411236	40.0	37.0	41.0	34.0	41.0
21	38.36240574480027	40.0	37.0	41.0	34.0	41.0
22	38.36825189939642	40.0	37.0	41.0	34.0	41.0
23	32.932794593963365	40.0	35.0	41.0	0.0	41.0
24	35.12128660161879	39.0	35.0	41.0	17.0	41.0
25	36.75629802780087	39.0	35.0	41.0	30.0	41.0
26	37.58558823066684	39.0	35.0	41.0	33.0	41.0
27	37.31100044517017	39.0	36.0	41.0	33.0	41.0
28	37.57390677928357	40.0	36.0	41.0	33.0	41.0
29	37.83243520048501	40.0	36.0	41.0	33.0	41.0
30	37.82618331837246	40.0	36.0	41.0	33.0	41.0
31	37.875782676299245	40.0	36.0	41.0	34.0	41.0
32	37.861462334149735	40.0	36.0	41.0	34.0	41.0
33	37.81253881943707	40.0	36.0	41.0	34.0	41.0
34	37.77712928836369	40.0	36.0	41.0	34.0	41.0
35	37.725970394521816	40.0	36.0	41.0	33.0	41.0
36	37.641371886384775	40.0	35.0	41.0	33.0	41.0
37	37.60153458511314	40.0	35.0	41.0	33.0	41.0
38	37.55728347368344	40.0	35.0	41.0	33.0	41.0
39	37.51225082159268	40.0	35.0	41.0	33.0	41.0
40	37.45388699591237	40.0	35.0	41.0	33.0	41.0
41	37.36517249180734	40.0	35.0	41.0	33.0	41.0
42	37.33029610131533	39.0	35.0	41.0	33.0	41.0
43	37.264110664562175	39.0	35.0	41.0	33.0	41.0
44	37.188946262930486	39.0	35.0	41.0	33.0	41.0
45	37.10988169198346	39.0	35.0	41.0	33.0	41.0
46	37.02311627539038	39.0	35.0	41.0	33.0	41.0
47	36.99060954631864	39.0	35.0	41.0	33.0	41.0
48	36.90524700296773	39.0	35.0	41.0	32.0	41.0
49	36.850734375668225	39.0	35.0	41.0	32.0	41.0
50	36.75856243495009	39.0	35.0	41.0	32.0	41.0
51	35.74758341733363	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	36.0
9	93.0
10	90.0
11	103.0
12	97.0
13	129.0
14	118.0
15	197.0
16	308.0
17	484.0
18	908.0
19	1601.0
20	2696.0
21	4376.0
22	6649.0
23	9423.0
24	13353.0
25	17983.0
26	22663.0
27	27527.0
28	33524.0
29	41703.0
30	54493.0
31	76144.0
32	106326.0
33	173758.0
34	284195.0
35	404846.0
36	368482.0
37	586031.0
38	837506.0
39	1436003.0
40	1032.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.16865013724049	22.52928893981472	24.337158458199983	10.964902464744805
2	30.569851055235002	24.401108737411867	25.46883022175856	19.560209985594568
3	32.325359343621066	23.955473233174107	26.065455747669837	17.653711675534986
4	30.589018411963444	25.886080311003106	24.645653186955297	18.879248090078157
5	26.226417226600923	30.50882573681867	23.40597946190028	19.85877757468012
6	27.85932090830669	34.20692901053673	21.91265402300659	16.021096058149993
7	82.0715192800342	6.113345333058859	6.976563308449746	4.838572078457198
8	82.80300322565563	5.5710974873922	6.3307452600677925	5.295154026884378
9	75.91706938428024	8.741599878215268	9.096628074172575	6.244702663331916
10	41.874492148142174	30.16567465439483	15.370270122345348	12.589563075117645
11	33.551915062683904	25.308755094583702	22.579234861278195	18.5600949814542
12	32.2723111910108	22.589671653207784	25.63754727855665	19.50046987722477
13	26.07418631246869	26.293093037463205	26.87897154833021	20.753749101737892
14	19.71906637910461	30.014130662873672	26.51953375238567	23.747269205636044
15	18.9791399330051	27.742632699599213	32.91888263838555	20.359344729010136
16	21.326997100078643	25.819869834812835	31.8016805672474	21.051452497861124
17	21.43834503945484	25.63185246852288	27.232448628714117	25.697353863308166
18	22.14452364243595	27.59879996835724	27.540234276064446	22.716442113142357
19	25.135451167447137	28.51553143103042	25.086014898243498	21.263002503278948
20	28.36002101540014	27.151967889248574	25.464531415740854	19.023479679610432
21	25.87989800750341	26.854153699155813	26.362117680479496	20.903830612861274
22	23.432459220617606	26.630704421410623	24.791568844824404	25.145267513147367
23	19.961882442723393	37.657895255824386	22.479830511817173	19.900391789635048
24	23.907211380047467	24.778938332298146	27.64087951798419	23.672970769670197
25	22.972642088280192	27.28622802152328	24.750242694190252	24.990887196006277
26	22.256890886331814	29.412253502806745	25.025654343644337	23.305201267217107
27	22.58297969744826	30.56213979495582	25.63127633988133	21.223604167714594
28	20.397258425382812	29.31617297243158	27.47530900992071	22.811259592264896
29	22.1031974918018	27.49833199678875	25.94748232891583	24.45098818249362
30	22.551336939750904	27.262939129128377	25.81304492628988	24.37267900483084
31	23.234315285512736	29.229000277206513	23.542610585122898	23.994073852157857
32	25.629791700689648	28.37340492691919	23.278034585888705	22.71876878650246
33	24.070543849926466	29.642572006662704	22.979954490269076	23.30692965314175
34	22.495518937902418	27.19989736046663	26.7031636774823	23.601420024148652
35	24.064716087129266	25.269068694698575	25.035913865222682	25.63030135294948
36	22.267216884291873	30.53211462921358	23.72078944691872	23.479879039575827
37	22.700354828766812	30.228406199941897	26.39103490652645	20.680204064764837
38	23.682255304316687	29.345688485913985	23.663154423969964	23.30890178579936
39	23.93980696588277	26.03217323922346	25.871300395467994	24.15671939942578
40	24.32833925822551	24.331153425051536	28.728854139960703	22.61165317676225
41	20.788405455406426	27.038891563947733	26.806623086228065	25.36607989441778
42	23.326916885244703	27.786905969822822	25.37133152857343	23.51484561635904
43	24.954192233298418	26.466264011836344	25.524603906019237	23.054939848846004
44	22.171357941855767	27.788922420068246	25.279749233361127	24.75997040471486
45	22.762177863763746	25.307048867452963	25.2253272355287	26.70544603325459
46	21.964461283158144	27.574957106114695	25.70495432961782	24.75562728110934
47	21.458354430351697	27.21937494030975	28.476000142702635	22.84627048663592
48	22.86876166245022	25.052156261155567	26.926036826585943	25.15304524980827
49	22.054137922094554	25.072963368633033	28.590716218752497	24.28218249051991
50	20.67960577732938	26.351791682519437	27.049040291556548	25.919562248594634
51	20.42236433887798	25.446383363532078	25.329052549801332	28.80219974778861
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1203.0
1	2810.0
2	4417.0
3	5385.5
4	6354.0
5	5231.5
6	4109.0
7	4143.5
8	4178.0
9	4475.0
10	4772.0
11	4857.0
12	4942.0
13	5278.5
14	5615.0
15	6383.0
16	7151.0
17	6663.0
18	6175.0
19	7428.0
20	8681.0
21	10108.0
22	11535.0
23	13154.5
24	14774.0
25	20929.0
26	34505.5
27	41927.0
28	49748.0
29	57569.0
30	63656.0
31	69743.0
32	83066.5
33	96390.0
34	108875.0
35	121360.0
36	123639.5
37	125919.0
38	136881.0
39	147843.0
40	168919.5
41	189996.0
42	214995.5
43	239995.0
44	276040.0
45	312085.0
46	347237.0
47	382389.0
48	409608.0
49	436827.0
50	437090.5
51	437354.0
52	385171.0
53	332988.0
54	293709.5
55	254431.0
56	240200.0
57	225969.0
58	221105.0
59	216241.0
60	215080.0
61	213919.0
62	192442.0
63	170965.0
64	142259.5
65	113554.0
66	90076.5
67	66599.0
68	57227.0
69	47855.0
70	43042.5
71	38230.0
72	31252.0
73	24274.0
74	20831.5
75	13351.5
76	9314.0
77	7595.0
78	5876.0
79	4197.5
80	2519.0
81	1871.0
82	1223.0
83	946.5
84	670.0
85	510.0
86	350.0
87	202.0
88	54.0
89	43.0
90	32.0
91	26.0
92	20.0
93	17.0
94	14.0
95	9.5
96	5.0
97	3.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4512881.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.270889227342268
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.54128680355686	9.210615545636873
2	12.15096088467287	3.4681003358187064
3	5.906642760068764	2.528791336032736
4	3.518543532809769	2.0085097999323898
5	2.352418518021724	1.6785552043518341
6	1.6515192109030348	1.4141188629374957
7	1.2544500480297565	1.2531482373666791
8	1.0099905424807627	1.1530770521925031
9	0.7615848686236169	0.9781643967612885
>10	4.695657570681994	12.45971662018863
>50	0.5336807155854724	5.512808658635452
>100	1.586090111795237	52.43543884692683
>500	0.028648186721967086	2.6621735526794454
>1k	0.007844146364348131	2.2079528903021717
>5k	3.4104984192817963E-4	0.33633987542010707
>10k+	3.4104984192817963E-4	0.6924887848169139
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	16770	0.3716029737987773	No Hit
CCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	11686	0.25894766558214144	No Hit
CTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGCT	8279	0.18345265474538328	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	5542	0.12280403582545164	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4770	0.10569744693024258	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.647638171713368E-5	0.0	0.0	0.22129987473633805	0.0
2	6.647638171713368E-5	0.0	0.0	1.2623643300144631	0.0
3	6.647638171713368E-5	0.0	0.0	1.7499021135279216	0.0
4	6.647638171713368E-5	0.0	0.0	2.263897496964799	0.0
5	6.647638171713368E-5	0.0	0.0	3.641443237701149	0.0
6	6.647638171713368E-5	0.0	0.0	4.3264601925023065	0.0
7	6.647638171713368E-5	0.0	0.0	5.089409625469849	0.0
8	8.863517562284491E-5	0.0	0.0	6.319887451053994	0.0
9	8.863517562284491E-5	0.0	0.0	6.736384141305742	0.0
10	8.863517562284491E-5	0.0	0.0	7.762779475018286	0.0
11	8.863517562284491E-5	0.0	0.0	9.237402891855558	0.0
12	8.863517562284491E-5	0.0	0.0	10.411951921621688	0.0
13	8.863517562284491E-5	0.0	0.0	10.815574352614217	0.0
14	1.1079396952855615E-4	0.0	0.0	10.99246800436351	0.0
15	1.1079396952855615E-4	0.0	0.0	11.304087123059526	0.0
16	1.1079396952855615E-4	0.0	0.0	11.904014309262752	0.0
17	1.1079396952855615E-4	0.0	0.0	12.62710007199392	0.0
18	1.1079396952855615E-4	0.0	0.0	13.397228954186915	0.0
19	1.1079396952855615E-4	0.0	0.0	13.87807478194085	0.0
20	1.1079396952855615E-4	0.0	0.0	14.361934205665959	0.0
21	1.1079396952855615E-4	0.0	0.0	14.971301038073019	0.0
22	1.1079396952855615E-4	0.0	0.0	15.623456501512006	0.0
23	1.1079396952855615E-4	0.0	0.0	16.2411107228398	0.0
24	1.1079396952855615E-4	0.0	0.0	16.811190013652034	0.0
25	1.1079396952855615E-4	0.0	0.0	17.299591990127816	0.0
26	1.1079396952855615E-4	0.0	0.0	17.749725729528432	0.0
27	1.1079396952855615E-4	0.0	0.0	18.17176211825661	0.0
28	1.1079396952855615E-4	0.0	0.0	18.64713472391583	0.0
29	1.1079396952855615E-4	0.0	0.0	19.13553670039161	0.0
30	1.1079396952855615E-4	0.0	0.0	19.679712361128068	0.0
31	1.7727035124568983E-4	0.0	0.0	20.223001670108296	0.0
32	1.7727035124568983E-4	0.0	0.0	20.716677439533637	0.0
33	1.9942914515140106E-4	0.0	0.0	21.18976768942057	0.0
34	1.9942914515140106E-4	0.0	0.0	21.65942332625212	0.0
35	2.215879390571123E-4	0.0	0.0	22.1761442413394	0.0
36	2.4374673296282353E-4	0.0	0.0	22.69649919862722	0.0
37	2.4374673296282353E-4	0.0	0.0	23.214882023257427	0.0
38	2.6590552686853473E-4	0.0	0.0	23.71128332433317	0.0
39	2.6590552686853473E-4	0.0	0.0	24.17446859334425	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGC	20	7.0354826E-4	45.000004	29
CCGTCTA	20	7.0354826E-4	45.000004	40
CGATCTA	40	6.8230293E-9	45.000004	18
ATGTTCG	20	7.0354826E-4	45.000004	22
GTGCGTA	30	2.1669202E-6	45.000004	1
TACGTAG	40	6.8230293E-9	45.000004	1
TCGTTAA	50	2.1827873E-11	45.0	36
TAACGGA	55	1.8189894E-12	45.0	11
ACGATAG	95	0.0	45.0	1
CCGTAAC	45	3.8562575E-10	45.0	28
ACGGTAC	35	1.2129931E-7	45.0	13
ACCGTAA	65	0.0	44.999996	38
TATCCGT	80	0.0	42.187504	35
CGGTAAT	120	0.0	41.250004	6
GACGTAG	170	0.0	41.02941	1
CCCGTAC	110	0.0	40.909092	22
CGTAGAT	55	6.184564E-11	40.909092	31
AAGTACG	150	0.0	40.500004	1
AACCGTA	50	1.0822987E-9	40.5	36
TAGTCCG	50	1.0822987E-9	40.5	38
>>END_MODULE