FastQCFastQC Report
Sat 18 Jun 2016
SRR3548321_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548321_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2555636
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC180770.7073386037761247TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC150880.5903814158197803TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT124890.4886846170581413TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC68600.26842633301456076TruSeq Adapter, Index 19 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC51050.19975458163838672TruSeq Adapter, Index 13 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG38160.14931703888973236No Hit
GCCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG36580.1431346248057235No Hit
AGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG32500.1271699099558779No Hit
GCTGTCTCTTATACACATCTGAAGCACAATCCCTCGTATGCCGTCTTCTGC30220.11824845165743479TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG29480.11535289063074709No Hit
GGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG27040.10580536508329043No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGT253.89215E-545.00000414
AGTCACG351.212702E-745.0000041
GTTTCGC351.212702E-745.00000435
GTTTCGA253.89215E-545.00000427
ACGTTAG1150.045.0000041
TTAGCGA302.1665492E-645.00000431
TTTCGCA351.212702E-745.00000431
TCGGGTA302.1665492E-645.00000422
TTCGTCG253.89215E-545.00000439
CGCGATC253.89215E-545.00000416
TAAGTCG253.89215E-545.00000411
CGCGACT502.1827873E-1145.00000435
ACTTACG600.045.00000410
CCTAACG302.1665492E-645.00000431
CCGTCGA502.1827873E-1145.00000435
ATCCGAC302.1665492E-645.00000443
CCCGTAG253.89215E-545.00000444
CTCGGTT502.1827873E-1145.00000414
CACTACG351.212702E-745.00000413
CTTGCGA302.1665492E-645.00000415
Produced by FastQC (version 0.11.5)