##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548321_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2555636
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13216866564722	33.0	31.0	34.0	30.0	34.0
2	32.259990468126134	33.0	31.0	34.0	30.0	34.0
3	32.28557157592083	34.0	31.0	34.0	30.0	34.0
4	35.89757500676935	37.0	35.0	37.0	35.0	37.0
5	35.85743470509885	37.0	35.0	37.0	35.0	37.0
6	35.85764561150336	37.0	35.0	37.0	35.0	37.0
7	36.07829988308194	37.0	35.0	37.0	35.0	37.0
8	35.994696819108825	37.0	35.0	37.0	35.0	37.0
9	37.78018544111916	39.0	38.0	39.0	35.0	39.0
10	37.55013859563725	39.0	37.0	39.0	35.0	39.0
11	37.474888833934095	39.0	37.0	39.0	35.0	39.0
12	37.278632794341604	39.0	37.0	39.0	35.0	39.0
13	37.12029256122546	39.0	37.0	39.0	33.0	39.0
14	38.334211523080754	40.0	38.0	41.0	34.0	41.0
15	38.21781349143618	40.0	37.0	41.0	34.0	41.0
16	38.165755999680705	40.0	37.0	41.0	34.0	41.0
17	38.163447767992	40.0	37.0	41.0	34.0	41.0
18	38.247476948986474	40.0	37.0	41.0	34.0	41.0
19	38.29462333446547	40.0	37.0	41.0	34.0	41.0
20	38.33396696556161	40.0	37.0	41.0	34.0	41.0
21	38.31903526167263	40.0	37.0	41.0	34.0	41.0
22	38.35058435551855	40.0	37.0	41.0	34.0	41.0
23	32.93108956048514	39.0	35.0	41.0	0.0	41.0
24	35.1608726751384	39.0	35.0	41.0	17.0	41.0
25	36.77404685174258	39.0	35.0	41.0	30.0	41.0
26	37.58146113139743	39.0	35.0	41.0	33.0	41.0
27	37.28920785276151	39.0	36.0	41.0	33.0	41.0
28	37.573612204554955	40.0	36.0	41.0	33.0	41.0
29	37.80154489919535	40.0	36.0	41.0	33.0	41.0
30	37.79353945554062	40.0	36.0	41.0	34.0	41.0
31	37.809405564798745	40.0	36.0	41.0	34.0	41.0
32	37.726485696711116	40.0	36.0	41.0	34.0	41.0
33	37.73967262943549	40.0	36.0	41.0	34.0	41.0
34	37.71007882186665	40.0	36.0	41.0	34.0	41.0
35	37.66489711367347	40.0	36.0	41.0	33.0	41.0
36	37.60381681898361	40.0	36.0	41.0	33.0	41.0
37	37.52554863055615	40.0	36.0	41.0	33.0	41.0
38	37.483485519847115	40.0	35.0	41.0	33.0	41.0
39	37.4539840571975	40.0	35.0	41.0	33.0	41.0
40	37.44350564790917	39.0	35.0	41.0	33.0	41.0
41	37.43171132352182	40.0	35.0	41.0	33.0	41.0
42	37.400374309956504	40.0	35.0	41.0	33.0	41.0
43	37.29122496317942	39.0	35.0	41.0	33.0	41.0
44	37.24237880511935	39.0	35.0	41.0	33.0	41.0
45	37.14988832525446	39.0	35.0	41.0	33.0	41.0
46	37.061587409161554	39.0	35.0	41.0	32.0	41.0
47	37.06426736827937	39.0	35.0	41.0	33.0	41.0
48	37.018248686432656	39.0	35.0	41.0	32.0	41.0
49	37.03847496278813	39.0	35.0	41.0	32.0	41.0
50	36.9469368877258	39.0	35.0	41.0	32.0	41.0
51	35.95408305408125	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	15.0
9	25.0
10	42.0
11	30.0
12	40.0
13	45.0
14	50.0
15	71.0
16	118.0
17	252.0
18	453.0
19	766.0
20	1249.0
21	2184.0
22	3358.0
23	5133.0
24	7174.0
25	9404.0
26	11963.0
27	14665.0
28	18356.0
29	24183.0
30	32211.0
31	44315.0
32	62154.0
33	100299.0
34	155794.0
35	240358.0
36	210772.0
37	333076.0
38	511778.0
39	764908.0
40	392.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.957540119171895	22.819994709731745	24.174099910941933	11.04836526015442
2	29.363806113233654	23.925199050255983	25.961169744048057	20.749825092462306
3	32.09502448705528	23.756004376210072	26.19324504741677	17.955726089317885
4	28.119106163788583	26.740545210663804	26.63826147385621	18.5020871516914
5	24.686614212665653	29.00624345564079	25.194198234803387	21.112944096890168
6	26.464645199864144	34.03900242444542	23.74884373204948	15.747508643640955
7	80.12451694998818	5.280564211804811	8.772532551584028	5.822386286622978
8	79.40137014817446	4.517583881272607	8.543352809242005	7.537693161310923
9	72.80039097899702	7.544188609019438	12.133418061101032	7.52200235088252
10	37.563761036391725	30.813386569918404	17.696925540256906	13.925926853432962
11	29.296229979543252	23.92046441668532	27.074434700403344	19.708870903368087
12	26.811721231036035	22.967550934483626	30.956012515084307	19.264715319396032
13	22.550864051062046	27.181100907954026	32.13415369011862	18.133881350865302
14	18.31634082474969	30.260177897008806	29.70434756749396	21.719133710747542
15	16.19796402930621	26.536486416688447	37.695548192309076	19.570001361696264
16	18.46796648662016	27.595674814410188	32.70907907072838	21.227279628241266
17	18.49833074819732	25.40494812250258	29.09792317841821	26.998797950881894
18	20.355598371599086	24.77766786819406	33.05341605768584	21.813317702521015
19	21.125582829479626	27.284323745635135	28.616007913490026	22.974085511395206
20	23.26845450604077	27.34888693068966	29.159473414836857	20.22318514843272
21	21.969404093540707	28.30273951376487	28.994856857549355	20.732999535145066
22	19.423579883833224	26.4788882297792	27.96951522047741	26.12801666591017
23	15.846035976954465	37.887203028913355	24.71228296987521	21.554478024256976
24	20.91995886738174	24.64611548749509	30.924278731399934	23.509646913723238
25	17.945787271739793	28.65693705989429	27.486660854675705	25.910614813690213
26	18.084656813411613	30.55435124563905	27.40488082027331	23.956111120676027
27	18.397181758278567	30.28299022239474	26.90187491489398	24.417953104432712
28	16.198707484164412	30.057332108328417	30.1258864720954	23.618073935411772
29	17.717820534692734	29.414087139170054	28.58216115284023	24.28593117329698
30	19.260215461043746	28.150526913848452	27.964428424079173	24.62482920102863
31	20.079463585581045	30.56311618712524	23.448487969335226	25.90893225795849
32	19.508098962450052	31.22565185339383	23.886891560456966	25.37935762369915
33	18.633091723547484	27.392946413338986	25.74325921218828	28.23070265092525
34	16.86472564950564	28.190908251409823	27.558893363530647	27.38547273555389
35	16.300012990895418	28.115506277106757	25.398530933200192	30.18594979879764
36	19.077951633174678	26.604336454800293	26.88907966549227	27.428632246532764
37	17.486488686182224	28.48598157171053	27.927020905950613	26.10050883615664
38	17.08670561848401	29.896784988159503	26.346905427846533	26.669603965509953
39	19.300909832229628	25.323637638536944	29.305777505090706	26.069675024142718
40	20.553787785114938	23.93678129436273	28.9856223656264	26.523808554895922
41	16.76768522590854	24.363250478550153	26.090217855750975	32.778846439790335
42	19.4155975264083	24.998317444268274	26.14781604266022	29.438268986663203
43	20.90031600744394	24.66939736331778	27.21009564742397	27.220190981814312
44	19.146819030566167	26.488748788951167	27.622243543290203	26.742188637192466
45	18.88966190803385	25.728233598211954	27.097247025789272	28.28485746796492
46	21.033903106702205	25.26177436849379	27.94826806321401	25.756054461589994
47	16.5834649378863	24.82732282688145	30.719359094957184	27.869853140275065
48	17.204601907313872	23.29467107209321	29.529635675816117	29.971091344776802
49	19.21204741207277	21.70849056751431	31.85402772538812	27.225434295024797
50	18.97429837425987	22.035258542296322	30.33827978632325	28.65216329712056
51	17.571829478063385	22.234152281467313	27.174488072636322	33.019530167832976
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1392.0
1	1757.5
2	2123.0
3	2613.0
4	3103.0
5	2664.0
6	2225.0
7	2235.5
8	2246.0
9	2397.5
10	2549.0
11	2867.5
12	3186.0
13	3198.5
14	3211.0
15	3557.5
16	3904.0
17	3712.0
18	3520.0
19	4097.5
20	4675.0
21	5238.0
22	5801.0
23	7251.0
24	8701.0
25	11029.0
26	17429.5
27	21502.0
28	25162.0
29	28822.0
30	32648.0
31	36474.0
32	40697.5
33	44921.0
34	51750.0
35	58579.0
36	71097.0
37	83615.0
38	86293.5
39	88972.0
40	101135.0
41	113298.0
42	143269.5
43	173241.0
44	194860.5
45	216480.0
46	251334.5
47	286189.0
48	319178.5
49	352168.0
50	333873.5
51	315579.0
52	259326.5
53	203074.0
54	168283.0
55	133492.0
56	112558.0
57	91624.0
58	82222.5
59	72821.0
60	63669.0
61	54517.0
62	49406.5
63	44296.0
64	37178.5
65	30061.0
66	23255.0
67	16449.0
68	14553.5
69	12658.0
70	9941.0
71	7224.0
72	5539.5
73	3855.0
74	3442.0
75	2171.0
76	1313.0
77	1050.0
78	787.0
79	518.5
80	250.0
81	215.0
82	180.0
83	96.5
84	13.0
85	10.5
86	8.0
87	18.5
88	29.0
89	73.0
90	117.0
91	60.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2555636.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.747600829335232
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.1517357097878	9.629931239787433
2	11.955605372379113	3.765442021545641
3	6.117549669124099	2.890101907289944
4	3.858074282928605	2.430216551099338
5	2.721982682255593	2.143234837226216
6	2.012830527216237	1.9018351007821015
7	1.5209688310658898	1.676612701784037
8	1.242877535767139	1.5657871450406984
9	1.0089325988826052	1.429944104781636
>10	6.420302277152483	18.79681692161023
>50	0.6624925699441774	7.462136714109258
>100	1.3013499624479004	40.08494896935556
>500	0.016865320698910002	1.7939635510534624
>1k	0.0070725538414783875	1.960812999019107
>5k	5.440426031906452E-4	0.5125426559458124
>10k+	8.160639047859678E-4	1.9556725795695877
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	18077	0.7073386037761247	TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	15088	0.5903814158197803	TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT	12489	0.4886846170581413	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	6860	0.26842633301456076	TruSeq Adapter, Index 19 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	5105	0.19975458163838672	TruSeq Adapter, Index 13 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	3816	0.14931703888973236	No Hit
GCCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	3658	0.1431346248057235	No Hit
AGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	3250	0.1271699099558779	No Hit
GCTGTCTCTTATACACATCTGAAGCACAATCCCTCGTATGCCGTCTTCTGC	3022	0.11824845165743479	TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	2948	0.11535289063074709	No Hit
GGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	2704	0.10580536508329043	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.347752183800823E-4	0.0	0.0	0.5786035256977128	0.0
2	2.347752183800823E-4	0.0	0.0	2.7121624519297742	0.0
3	2.347752183800823E-4	0.0	0.0	3.6872621922683826	0.0
4	2.347752183800823E-4	0.0	0.0	4.7660543207248605	0.0
5	2.347752183800823E-4	0.0	0.0	7.23675046055072	0.0
6	2.347752183800823E-4	0.0	0.0	8.501954112400984	0.0
7	2.347752183800823E-4	0.0	0.0	9.915457443861332	0.0
8	2.347752183800823E-4	0.0	0.0	12.320338264134643	0.0
9	2.347752183800823E-4	0.0	0.0	13.212757998400399	0.0
10	2.347752183800823E-4	0.0	0.0	15.085129494184617	0.0
11	2.347752183800823E-4	0.0	0.0	17.60904135017663	0.0
12	2.347752183800823E-4	0.0	0.0	19.645638111217718	0.0
13	2.347752183800823E-4	0.0	0.0	20.329538322358896	0.0
14	2.347752183800823E-4	0.0	0.0	20.6190552958246	0.0
15	2.347752183800823E-4	0.0	0.0	21.13599119749448	0.0
16	2.347752183800823E-4	0.0	0.0	22.212748607391664	0.0
17	2.347752183800823E-4	0.0	0.0	23.503386241233102	0.0
18	2.347752183800823E-4	0.0	0.0	25.04233779771454	0.0
19	2.347752183800823E-4	0.0	0.0	25.85912078245885	0.0
20	2.347752183800823E-4	0.0	0.0	26.654617480736693	0.0
21	3.130336245067764E-4	0.0	0.0	27.58092310485531	0.0
22	3.521628275701234E-4	0.0	0.0	28.54451885949329	0.0
23	3.912920306334705E-4	0.0	0.0	29.465894204025926	0.0
24	3.912920306334705E-4	0.0	0.0	30.317697825511928	0.0
25	3.912920306334705E-4	0.0	0.0	31.023314744353264	0.0
26	3.912920306334705E-4	0.0	0.0	31.664564124155397	0.0
27	3.912920306334705E-4	0.0	0.0	32.28413592546043	0.0
28	3.912920306334705E-4	0.0	0.0	32.94056743605115	0.0
29	3.912920306334705E-4	0.0	0.0	33.65389280789596	0.0
30	3.912920306334705E-4	0.0	0.0	34.490396910984195	0.0
31	4.3042123369681754E-4	0.0	0.0	35.21890441361759	0.0
32	4.3042123369681754E-4	0.0	0.0	35.89709176111152	0.0
33	4.3042123369681754E-4	0.0	0.0	36.5538754345298	0.0
34	4.3042123369681754E-4	0.0	0.0	37.18596858081511	0.0
35	4.3042123369681754E-4	0.0	0.0	37.88861168022363	0.0
36	4.3042123369681754E-4	0.0	0.0	38.534478305987236	0.0
37	4.3042123369681754E-4	0.0	0.0	39.17071914779726	0.0
38	4.695504367601646E-4	0.0	0.0	39.77162631924108	0.0
39	5.086796398235116E-4	0.0	0.0	40.411467047732934	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGGT	25	3.89215E-5	45.000004	14
AGTCACG	35	1.212702E-7	45.000004	1
GTTTCGC	35	1.212702E-7	45.000004	35
GTTTCGA	25	3.89215E-5	45.000004	27
ACGTTAG	115	0.0	45.000004	1
TTAGCGA	30	2.1665492E-6	45.000004	31
TTTCGCA	35	1.212702E-7	45.000004	31
TCGGGTA	30	2.1665492E-6	45.000004	22
TTCGTCG	25	3.89215E-5	45.000004	39
CGCGATC	25	3.89215E-5	45.000004	16
TAAGTCG	25	3.89215E-5	45.000004	11
CGCGACT	50	2.1827873E-11	45.000004	35
ACTTACG	60	0.0	45.000004	10
CCTAACG	30	2.1665492E-6	45.000004	31
CCGTCGA	50	2.1827873E-11	45.000004	35
ATCCGAC	30	2.1665492E-6	45.000004	43
CCCGTAG	25	3.89215E-5	45.000004	44
CTCGGTT	50	2.1827873E-11	45.000004	14
CACTACG	35	1.212702E-7	45.000004	13
CTTGCGA	30	2.1665492E-6	45.000004	15
>>END_MODULE