FastQCFastQC Report
Sat 18 Jun 2016
SRR3548320_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548320_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2632957
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC135810.5158078920392547No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC132600.5036162763007523No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT85830.3259832955874327No Hit
TCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC47940.18207665373950277No Hit
ACTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC37900.1439446219592648No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG28640.10877503886314892No Hit
ACCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG28430.10797745652511605No Hit
GCCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG27850.10577461006769195No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA253.8921826E-545.025
CGAACGC453.8562575E-1045.020
ACACGAA351.2127202E-745.019
CGTATTA351.2127202E-745.037
CACGGAT351.2127202E-745.040
ACGTTAC453.8562575E-1045.038
CTATCGA207.0349476E-445.037
ATTACGA453.8562575E-1045.014
TTGTCGT453.8562575E-1045.040
TAACGAT453.8562575E-1045.012
TCGATAT551.8189894E-1245.037
ATCCGAT351.2127202E-745.09
TCAATCG253.8921826E-545.035
CCGTACT453.8562575E-1045.039
GCCGAAT253.8921826E-545.019
CTACGGT253.8921826E-545.045
CGTAGCA207.0349476E-445.032
TGCGAAC253.8921826E-545.035
GTCACGA253.8921826E-545.021
CCGAGTA502.1827873E-1145.022