##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548320_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2632957
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18029690572235	33.0	31.0	34.0	30.0	34.0
2	32.314120207811975	34.0	31.0	34.0	30.0	34.0
3	32.320173857757645	34.0	31.0	34.0	30.0	34.0
4	35.93904040210303	37.0	35.0	37.0	35.0	37.0
5	35.89796567129657	37.0	35.0	37.0	35.0	37.0
6	35.8733074638135	37.0	35.0	37.0	35.0	37.0
7	36.06123989111862	37.0	35.0	37.0	35.0	37.0
8	35.97285371542338	37.0	35.0	37.0	35.0	37.0
9	37.76030068094541	39.0	38.0	39.0	35.0	39.0
10	37.53993209915696	39.0	37.0	39.0	35.0	39.0
11	37.42761693411628	39.0	37.0	39.0	35.0	39.0
12	37.079823559594786	39.0	35.0	39.0	34.0	39.0
13	36.849320364897714	39.0	35.0	39.0	33.0	39.0
14	38.005274298061074	40.0	36.0	41.0	33.0	41.0
15	37.91898234570485	40.0	36.0	41.0	33.0	41.0
16	37.84100423971983	40.0	36.0	41.0	33.0	41.0
17	37.84641602578394	40.0	36.0	41.0	33.0	41.0
18	37.92877247900365	40.0	36.0	41.0	34.0	41.0
19	37.991183296954716	40.0	36.0	41.0	34.0	41.0
20	38.0086378167209	40.0	36.0	41.0	34.0	41.0
21	37.968887072595564	40.0	35.0	41.0	34.0	41.0
22	38.00869820509792	40.0	35.0	41.0	34.0	41.0
23	32.82320789895164	39.0	35.0	41.0	0.0	41.0
24	34.8976508161736	39.0	35.0	41.0	17.0	41.0
25	36.45554598878751	39.0	35.0	41.0	30.0	41.0
26	37.223725643829354	39.0	35.0	41.0	32.0	41.0
27	36.965160843872496	39.0	35.0	41.0	33.0	41.0
28	37.23138091507001	39.0	35.0	41.0	33.0	41.0
29	37.47114783872277	39.0	35.0	41.0	33.0	41.0
30	37.489037990365965	39.0	35.0	41.0	33.0	41.0
31	37.50386542583111	39.0	35.0	41.0	33.0	41.0
32	37.41592817505185	39.0	35.0	41.0	33.0	41.0
33	37.40684409202277	39.0	35.0	41.0	33.0	41.0
34	37.40182008289539	39.0	35.0	41.0	33.0	41.0
35	37.27077312694434	39.0	35.0	41.0	33.0	41.0
36	37.21793671525969	39.0	35.0	41.0	33.0	41.0
37	37.173481374743304	39.0	35.0	41.0	33.0	41.0
38	37.10054816694689	39.0	35.0	41.0	33.0	41.0
39	37.02737226623906	39.0	35.0	41.0	33.0	41.0
40	36.97598365639849	39.0	35.0	41.0	32.0	41.0
41	36.90184799827722	39.0	35.0	41.0	32.0	41.0
42	36.87951113519894	39.0	35.0	41.0	32.0	41.0
43	36.794990575235374	39.0	35.0	41.0	32.0	41.0
44	36.72577675974199	39.0	35.0	41.0	32.0	41.0
45	36.633387860113174	39.0	35.0	41.0	32.0	41.0
46	36.51435287397401	38.0	35.0	40.0	31.0	41.0
47	36.49378702348728	38.0	35.0	40.0	32.0	41.0
48	36.401501809562404	38.0	35.0	40.0	31.0	41.0
49	36.36489961666673	38.0	35.0	40.0	32.0	41.0
50	36.19375781678166	38.0	35.0	40.0	31.0	41.0
51	35.068131762121446	36.0	34.0	39.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	29.0
9	58.0
10	74.0
11	47.0
12	47.0
13	54.0
14	53.0
15	96.0
16	181.0
17	308.0
18	607.0
19	1082.0
20	1858.0
21	2993.0
22	4551.0
23	6534.0
24	9314.0
25	12145.0
26	15085.0
27	18354.0
28	22784.0
29	28679.0
30	37504.0
31	51262.0
32	72743.0
33	121184.0
34	197408.0
35	295380.0
36	212874.0
37	324245.0
38	470949.0
39	724072.0
40	403.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.47150902958157	23.11154340917835	24.681223430538367	10.735724130701717
2	31.141412487936567	23.438969948996508	25.674137481166614	19.74548008190031
3	31.464053533726528	24.924903824863073	25.987397439456856	17.623645201953543
4	30.243448715645567	26.020668016986225	25.045528658462707	18.6903546089055
5	25.58021266583541	30.33205631538988	23.81512497165734	20.272606047117367
6	27.008416772472927	34.336033592648874	22.998476617734358	15.657073017143844
7	81.20611160759556	6.054219647339474	7.47053597912917	5.269132765935789
8	80.83527380052162	5.347941496955704	7.632065392636492	6.1847193098861855
9	74.28340075436097	8.418975319384252	10.35565715657339	6.941966769681389
10	43.416508511153054	27.266681529550237	16.109264222697142	13.207545736599572
11	37.03976175835762	23.18750363184815	21.533393822990654	18.23934078680358
12	33.89918635207487	21.182609514701532	26.003501006662848	18.914703126560745
13	25.093193698188003	27.748345301499416	27.594525850593076	19.563935149719498
14	18.129806145713736	31.97036639793206	27.52186989760942	22.37795755874479
15	18.37857587495732	25.765745509706388	36.647655088936126	19.208023526400165
16	19.801500746119288	23.823974337598372	35.06369454571419	21.310830370568148
17	20.14028333922658	24.613390951694235	26.934051714479196	28.31227399459999
18	21.925842313414158	25.414961201417263	29.60249635675782	23.056700128410757
19	24.76546331747917	26.45257784308669	26.054318395628943	22.727640443805196
20	26.393025028513566	25.381386782997218	26.499483280585288	21.726104907903927
21	24.43028883494869	27.525781849076914	27.008986474142947	21.034942841831448
22	22.29075522311986	25.671250992705158	25.405694054251548	26.63229972992343
23	18.738171569076137	36.611953784281326	22.74913718682075	21.90073745982179
24	23.334182821823525	23.37850561175135	28.95288453248572	24.334427033939406
25	21.6575128268331	25.67968257742151	27.05782889731963	25.604975698425765
26	22.104158936131505	27.69562890696658	26.584368829418786	23.61584332748313
27	22.39804903764095	28.41865628644904	25.576794455815268	23.606500220094745
28	20.18152974013628	27.220801555057676	29.02440108212933	23.57326762267671
29	21.942705482846854	24.441189126901808	30.003794213122358	23.612311177128984
30	22.58430350362729	24.981760051531417	26.900933057395164	25.53300338744613
31	26.161156448814015	26.43203060285451	23.85169981887285	23.555113129458626
32	25.076330528755314	28.40175513690501	24.224398651402208	22.297515682937473
33	22.856013220117152	28.579691958509006	24.356911259849667	24.207383561524175
34	22.01494365460583	25.618040856724967	28.27839573528926	24.088619753379945
35	21.09179906849979	25.808663035514822	27.47788892868361	25.62164896730178
36	25.132882914532978	26.446576985495774	25.082483306791563	23.338056793179685
37	22.059532305313002	27.389547189718634	28.729181676723165	21.821738828245202
38	21.363204944098975	28.95444171705045	25.194144834116166	24.48820850473441
39	22.766797938591477	25.805358765828686	27.672650939608967	23.755192355970873
40	23.227116887970446	24.57054938610847	28.65781704752489	23.544516678396192
41	19.029137202012794	26.725768783918614	26.323901225884054	27.92119278818454
42	22.486694617496603	26.510497512872412	25.508999957082473	25.493807912548515
43	24.27677322493303	24.16928950985527	26.318811890965176	25.23512537424652
44	21.993826712703626	25.08631929803639	27.329234772918813	25.59061921634117
45	21.450976981393925	24.304498706207507	26.422383654575444	27.822140657823123
46	22.680317225081915	26.35815928630813	27.020038686541405	23.94148480206855
47	21.471296340958094	24.61699906227105	29.71677851176453	24.194926085006326
48	21.331339630688994	23.010858134029537	29.236785864714083	26.42101637056739
49	21.59818789292799	22.332799206367593	30.931838233590597	25.137174667113822
50	20.253729931783923	23.822455133144977	29.533372554128306	26.390442380942797
51	19.62382978529463	23.406534933916507	26.354968956956	30.61466632383286
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1241.0
1	1977.0
2	2713.0
3	3293.0
4	3873.0
5	3236.5
6	2600.0
7	2705.5
8	2811.0
9	3105.0
10	3399.0
11	3694.0
12	3989.0
13	4218.5
14	4448.0
15	4073.5
16	3699.0
17	3833.0
18	3967.0
19	3848.5
20	3730.0
21	4449.5
22	5169.0
23	6880.0
24	8591.0
25	10129.5
26	15874.0
27	20080.0
28	24143.0
29	28206.0
30	33214.0
31	38222.0
32	41957.5
33	45693.0
34	48557.0
35	51421.0
36	56218.5
37	61016.0
38	63518.0
39	66020.0
40	88029.0
41	110038.0
42	136400.0
43	162762.0
44	185833.5
45	208905.0
46	225885.0
47	242865.0
48	266407.0
49	289949.0
50	284526.5
51	279104.0
52	237098.5
53	195093.0
54	173905.0
55	152717.0
56	136067.0
57	119417.0
58	118835.5
59	118254.0
60	112276.0
61	106298.0
62	99518.0
63	92738.0
64	76525.0
65	60312.0
66	51742.0
67	43172.0
68	35019.0
69	26866.0
70	22912.0
71	18958.0
72	16267.0
73	13576.0
74	10490.0
75	5999.0
76	4594.0
77	4294.5
78	3995.0
79	2824.0
80	1653.0
81	1522.5
82	1392.0
83	767.0
84	142.0
85	99.5
86	57.0
87	47.0
88	37.0
89	56.0
90	75.0
91	48.0
92	21.0
93	12.0
94	3.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2632957.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.649755718569574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.62433030641144	9.320808967465103
2	11.958067356158555	3.503655312678479
3	5.8898635822081875	2.5885518808514725
4	3.5679887702468083	2.090806555628608
5	2.4014892989183547	1.7590615794956403
6	1.7648075505495722	1.5512399703503015
7	1.3385826849323137	1.3726936540355763
8	1.0854455270903804	1.2721209454150455
9	0.826743985493081	1.0900437686343236
>10	5.526602694888855	15.768048848734267
>50	0.6318771997005727	6.3867817811433785
>100	1.345713370335661	47.04706833253139
>500	0.03144063433586143	2.933808259172893
>1k	0.006233918876938042	1.9087403308660538
>5k	2.710399511712192E-4	0.34080252667561195
>10k+	5.420799023424384E-4	1.0657672863218104
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	13581	0.5158078920392547	No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	13260	0.5036162763007523	No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT	8583	0.3259832955874327	No Hit
TCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	4794	0.18207665373950277	No Hit
ACTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	3790	0.1439446219592648	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2864	0.10877503886314892	No Hit
ACCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG	2843	0.10797745652511605	No Hit
GCCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG	2785	0.10577461006769195	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7980111334898365E-5	0.0	0.0	0.3829154824784453	0.0
2	3.7980111334898365E-5	0.0	0.0	1.9852963796978074	0.0
3	3.7980111334898365E-5	0.0	0.0	2.696284063887105	0.0
4	3.7980111334898365E-5	0.0	0.0	3.4142980686733586	0.0
5	3.7980111334898365E-5	0.0	0.0	5.239014537647216	0.0
6	3.7980111334898365E-5	0.0	0.0	6.181528980534053	0.0
7	3.7980111334898365E-5	0.0	0.0	7.1453882459911044	0.0
8	3.7980111334898365E-5	0.0	0.0	8.859582590980407	0.0
9	3.7980111334898365E-5	0.0	0.0	9.455148716822949	0.0
10	3.7980111334898365E-5	0.0	0.0	10.75691703282659	0.0
11	3.7980111334898365E-5	0.0	0.0	12.472820482825963	0.0
12	3.7980111334898365E-5	0.0	0.0	13.871741923624274	0.0
13	3.7980111334898365E-5	0.0	0.0	14.384663327202077	0.0
14	3.7980111334898365E-5	0.0	0.0	14.5987572147969	0.0
15	3.7980111334898365E-5	0.0	0.0	14.97605164079778	0.0
16	7.596022266979673E-5	0.0	0.0	15.767291300237718	0.0
17	7.596022266979673E-5	0.0	0.0	16.67129391023097	0.0
18	1.139403340046951E-4	0.0	0.0	17.82334462735244	0.0
19	1.139403340046951E-4	0.0	0.0	18.393578018934605	0.0
20	1.5192044533959346E-4	0.0	0.0	18.96244412650871	0.0
21	1.5192044533959346E-4	0.0	0.0	19.670659262570563	0.0
22	1.8990055667449183E-4	0.0	0.0	20.366113081223887	0.0
23	1.8990055667449183E-4	0.0	0.0	21.06661825468475	0.0
24	1.8990055667449183E-4	0.0	0.0	21.679617251630013	0.0
25	1.8990055667449183E-4	0.0	0.0	22.187411340177604	0.0
26	1.8990055667449183E-4	0.0	0.0	22.70006688297606	0.0
27	2.278806680093902E-4	0.0	0.0	23.16528526671723	0.0
28	2.278806680093902E-4	0.0	0.0	23.666204955113205	0.0
29	2.278806680093902E-4	0.0	0.0	24.16875778829658	0.0
30	2.278806680093902E-4	0.0	0.0	24.779060197337063	0.0
31	2.6586077934428857E-4	0.0	0.0	25.37584168674232	0.0
32	2.6586077934428857E-4	0.0	0.0	25.903499373518063	0.0
33	2.6586077934428857E-4	0.0	0.0	26.41148336262233	0.0
34	2.6586077934428857E-4	0.0	0.0	26.92011301361929	0.0
35	2.6586077934428857E-4	0.0	0.0	27.518375727366607	0.0
36	3.7980111334898366E-4	0.0	0.0	28.051578510397245	0.0
37	3.7980111334898366E-4	0.0	0.0	28.570348851120624	0.0
38	3.7980111334898366E-4	0.0	0.0	29.10628620216737	0.0
39	3.7980111334898366E-4	0.0	0.0	29.63933706475267	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	25	3.8921826E-5	45.0	25
CGAACGC	45	3.8562575E-10	45.0	20
ACACGAA	35	1.2127202E-7	45.0	19
CGTATTA	35	1.2127202E-7	45.0	37
CACGGAT	35	1.2127202E-7	45.0	40
ACGTTAC	45	3.8562575E-10	45.0	38
CTATCGA	20	7.0349476E-4	45.0	37
ATTACGA	45	3.8562575E-10	45.0	14
TTGTCGT	45	3.8562575E-10	45.0	40
TAACGAT	45	3.8562575E-10	45.0	12
TCGATAT	55	1.8189894E-12	45.0	37
ATCCGAT	35	1.2127202E-7	45.0	9
TCAATCG	25	3.8921826E-5	45.0	35
CCGTACT	45	3.8562575E-10	45.0	39
GCCGAAT	25	3.8921826E-5	45.0	19
CTACGGT	25	3.8921826E-5	45.0	45
CGTAGCA	20	7.0349476E-4	45.0	32
TGCGAAC	25	3.8921826E-5	45.0	35
GTCACGA	25	3.8921826E-5	45.0	21
CCGAGTA	50	2.1827873E-11	45.0	22
>>END_MODULE