FastQCFastQC Report
Sat 18 Jun 2016
SRR3548311_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548311_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3335519
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTC461011.3821237414627228No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCG413821.240646508084649No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGC351941.0551281524704252No Hit
GCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC321410.963598168680796Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCT149420.44796626851773297Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC128400.3849475898653253Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC116940.35059011805958834Illumina Single End Adapter 2 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTT99070.2970152471024749No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCT89560.2685039419652534No Hit
GAACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCT86470.2592400163212981No Hit
ACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC79510.23837369836598143Illumina Single End Adapter 2 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTC76350.2288999103287974No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCACAGAT53620.16075459321323007No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCC52070.15610764022030754No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCACAGATCGTA48950.14675377355068284No Hit
AGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG45850.13745986756483775No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37870.11353555473675911No Hit
ACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG35830.10741956499123524No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAAG1200.045.0000041
CGCCCAT302.1667493E-645.00000426
TAGACCG207.03522E-445.01
GAGTACG950.045.01
TTCGTAG1600.045.01
CACGAAA253.8924074E-545.05
CGTTCGC207.03522E-445.015
CCGCTAT253.8924074E-545.030
CCTCCGT207.03522E-445.026
TCGCTAG253.8924074E-545.01
ACGGACG453.8562575E-1045.01
GTCCGCG406.8212103E-945.01
CGTACTA253.8924074E-545.036
CGTAAGA207.03522E-445.031
ATCGGTG253.8924074E-545.02
CGACTTG253.8924074E-545.08
CAATCGT207.03522E-445.015
TGTCGAC406.8212103E-945.01
TCGCGGT207.03522E-445.017
TCGCGAC207.03522E-445.018
Produced by FastQC (version 0.11.5)