##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548311_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3335519
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.571199264642175	31.0	31.0	33.0	30.0	34.0
2	31.57056098316334	31.0	31.0	34.0	30.0	34.0
3	31.496912474490475	33.0	31.0	34.0	28.0	34.0
4	35.29571020282001	35.0	35.0	37.0	33.0	37.0
5	35.528223044149954	37.0	35.0	37.0	33.0	37.0
6	35.50175639832962	37.0	35.0	37.0	33.0	37.0
7	35.787668425813195	37.0	35.0	37.0	35.0	37.0
8	35.53037293446687	37.0	35.0	37.0	33.0	37.0
9	37.33911514220126	39.0	37.0	39.0	34.0	39.0
10	36.76394408186552	38.0	35.0	39.0	32.0	39.0
11	36.97016776099911	39.0	37.0	39.0	33.0	39.0
12	36.949789223206345	39.0	35.0	39.0	34.0	39.0
13	36.7933224184902	39.0	35.0	39.0	33.0	39.0
14	37.8018224450228	39.0	36.0	41.0	33.0	41.0
15	38.001740059043286	40.0	37.0	41.0	33.0	41.0
16	37.96286035246689	39.0	36.0	41.0	34.0	41.0
17	37.85186862973948	39.0	36.0	41.0	34.0	41.0
18	37.87448130260988	39.0	36.0	41.0	34.0	41.0
19	37.73778053730169	39.0	36.0	40.0	34.0	41.0
20	37.73700914310486	39.0	36.0	41.0	34.0	41.0
21	37.79590582455084	39.0	36.0	41.0	34.0	41.0
22	37.91470202987901	39.0	36.0	41.0	34.0	41.0
23	37.877883471807536	39.0	36.0	41.0	34.0	41.0
24	37.87934741190202	39.0	36.0	41.0	34.0	41.0
25	37.6262242847365	39.0	35.0	41.0	33.0	41.0
26	37.73475252277082	39.0	36.0	41.0	34.0	41.0
27	37.80732143933223	40.0	36.0	41.0	34.0	41.0
28	37.746565976689084	40.0	36.0	41.0	34.0	41.0
29	37.750261653433846	40.0	36.0	41.0	34.0	41.0
30	37.60315710988305	40.0	36.0	41.0	33.0	41.0
31	37.585072068244855	40.0	36.0	41.0	33.0	41.0
32	37.53008182534712	40.0	36.0	41.0	33.0	41.0
33	37.489729184573676	40.0	36.0	41.0	33.0	41.0
34	37.4730469231325	40.0	36.0	41.0	33.0	41.0
35	37.41550685215704	40.0	36.0	41.0	33.0	41.0
36	37.36892909319359	40.0	35.0	41.0	33.0	41.0
37	37.26838761823872	39.0	35.0	41.0	33.0	41.0
38	37.222174420232655	39.0	35.0	41.0	33.0	41.0
39	37.14477477118253	39.0	35.0	41.0	33.0	41.0
40	37.051310455734175	39.0	35.0	41.0	32.0	41.0
41	36.93637481903116	39.0	35.0	41.0	32.0	41.0
42	36.98652803356839	39.0	35.0	41.0	32.0	41.0
43	36.89163425541872	39.0	35.0	40.0	32.0	41.0
44	36.87030384177095	39.0	35.0	40.0	32.0	41.0
45	36.85948663461368	39.0	35.0	40.0	32.0	41.0
46	36.765438901712145	39.0	35.0	40.0	32.0	41.0
47	36.681397707523175	39.0	35.0	40.0	31.0	41.0
48	36.64518175432369	39.0	35.0	40.0	31.0	41.0
49	36.641745707339695	39.0	35.0	40.0	31.0	41.0
50	36.53404852438256	39.0	35.0	40.0	31.0	41.0
51	33.71676641626086	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	13.0
11	15.0
12	55.0
13	68.0
14	103.0
15	171.0
16	269.0
17	527.0
18	1050.0
19	1952.0
20	3212.0
21	4793.0
22	7119.0
23	10228.0
24	13548.0
25	17023.0
26	20440.0
27	23063.0
28	26639.0
29	31516.0
30	40613.0
31	54718.0
32	77225.0
33	116606.0
34	239954.0
35	306558.0
36	255948.0
37	430827.0
38	851945.0
39	799289.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.64832399395716	24.783339564247722	28.529593145774317	9.0387432960208
2	27.95478005072074	29.791345814549402	28.338588387594253	13.915285747135602
3	26.783178270008356	29.39641477083476	30.197879250575397	13.622527708581483
4	24.42870210003301	26.116925132190822	34.185144800554276	15.269227967221893
5	24.846718007002806	30.592060785742785	28.030420453308764	16.530800753945638
6	20.005492398634214	41.77041114141458	26.200180541618863	12.02391591833235
7	79.72192633290351	4.049085014955693	12.116225391011113	4.112763261129677
8	76.15993193263178	6.650958966205859	9.36693809868869	7.822171002473678
9	71.07661506350286	5.608992183825066	12.608382683474446	10.706010069197628
10	38.685523901977476	26.319862066443033	19.70481954982118	15.289794481758312
11	30.20786270442471	23.409940102274938	26.83831211874374	19.543885074556613
12	26.674139766555072	19.548891791652213	32.18416684180183	21.592801599990885
13	22.131068658280764	28.888967504007624	34.219622193727574	14.76034164398404
14	17.60841416283343	34.205201649278564	28.339128033748274	19.847256154139732
15	15.001143750043097	25.337286341345976	42.862055350306804	16.79951455830412
16	15.02227989107542	25.852468536380695	35.76399954549802	23.361252027045868
17	15.791095778498038	26.50244234855205	29.057576946795987	28.648884926153922
18	17.329387120864848	26.47093300922585	33.52566722000384	22.674012649905457
19	19.66278711049165	29.515316806769803	28.106330678973794	22.71556540376475
20	21.702499670965747	28.413599203002594	28.815665568087006	21.068235557944657
21	19.185949772734016	31.7892058177453	29.238328428049726	19.786515981470952
22	17.561434967092076	26.955805078609956	26.15961713904193	29.323142815256038
23	16.25513151026872	31.291442201348573	26.746362410167652	25.707063878215052
24	20.331348734634698	25.507874486699073	29.849297815422425	24.3114789632438
25	15.92450829990775	31.07273560726232	27.35805132574571	25.644704767084225
26	15.574727651079186	29.862908890640405	29.18379418615214	25.378569272128264
27	20.3113218662523	29.365684920397694	27.357691561643033	22.965301651706977
28	14.500921745611404	27.262054271014495	32.24802497002715	25.988999013346948
29	16.93319690279084	24.52146127784012	29.07364641004893	29.47169540932011
30	18.774889305082656	27.370733010365107	28.89838133136103	24.95599635319121
31	19.84755595755863	28.96595702198069	22.016154007817075	29.170333012643614
32	21.638941346159324	31.402639289417927	21.455611555503058	25.50280780891969
33	18.603701552891767	28.87943375528666	23.56865603223966	28.948208659581915
34	16.942550769460464	28.57519324578874	24.336452588038025	30.14580339671278
35	18.01317875868793	28.008324941335967	25.49447327387432	28.484023026101784
36	21.102593029750395	26.626441042608363	24.93617934720204	27.334786580439207
37	16.904205912183382	31.77280657073157	29.077933598939175	22.24505391814587
38	18.817791174326995	30.569215765222747	22.319704969451532	28.29328809099873
39	19.63190735834513	32.348878840144515	24.395004195748847	23.6242096057615
40	22.940507908964094	25.557521932868614	26.65750067680622	24.844469481361074
41	18.282552130567986	29.658233096558583	23.952584290480733	28.106630482392696
42	22.352443502795218	25.848541111593125	27.574629315557793	24.224386070053868
43	21.37082714863864	26.338749681833622	27.53622449759693	24.754198671930816
44	19.30617094371221	27.339793297534808	25.81052603807683	27.543509720676152
45	18.218394198923765	26.165313404000994	25.569364167915097	30.04692822916014
46	24.238416870058302	27.23956901459713	25.32688316271021	23.195130952634358
47	17.75912534151357	25.14817034470498	31.611122586919755	25.481581726861695
48	20.271687854273953	24.137652940966607	27.413694840293218	28.176964364466215
49	20.583033704799764	21.22701144859316	32.26598319481916	25.923971651787923
50	19.499813971978575	22.923269212377445	29.46854747342168	28.108369342222307
51	18.528630776799652	23.241030856067677	24.206487805945642	34.02385056118703
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	944.0
1	1148.0
2	1352.0
3	3916.5
4	6481.0
5	4927.5
6	3374.0
7	3826.5
8	4279.0
9	5132.0
10	5985.0
11	6834.0
12	7683.0
13	8119.0
14	8555.0
15	8336.0
16	8117.0
17	8193.5
18	8270.0
19	8193.0
20	8116.0
21	7981.0
22	7846.0
23	8510.5
24	9175.0
25	11958.5
26	16010.0
27	17278.0
28	23279.0
29	29280.0
30	33246.0
31	37212.0
32	45743.5
33	54275.0
34	60049.0
35	65823.0
36	75252.0
37	84681.0
38	97403.0
39	110125.0
40	134617.5
41	159110.0
42	200857.5
43	242605.0
44	283672.5
45	324740.0
46	457703.0
47	590666.0
48	548820.0
49	506974.0
50	461123.5
51	415273.0
52	321845.0
53	228417.0
54	181729.5
55	135042.0
56	109850.5
57	84659.0
58	71467.5
59	58276.0
60	48588.5
61	38901.0
62	32142.0
63	25383.0
64	19731.0
65	14079.0
66	11323.0
67	8567.0
68	6078.0
69	3589.0
70	2656.0
71	1723.0
72	1620.0
73	1517.0
74	1177.5
75	764.0
76	690.0
77	496.5
78	303.0
79	242.0
80	181.0
81	144.5
82	108.0
83	87.5
84	67.0
85	60.0
86	53.0
87	49.5
88	46.0
89	41.5
90	37.0
91	36.0
92	35.0
93	31.0
94	27.0
95	20.5
96	14.0
97	9.0
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3335519.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.13064857006725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.32836286735282	12.949716659704436
2	12.935075003445789	5.207828982436571
3	6.329288373375648	3.822380398291133
4	3.641962476760436	2.932602669001442
5	2.3904395698145158	2.4060549453959377
6	1.621230384575888	1.9581851473807304
7	1.1411551436920635	1.6080535213112666
8	0.8663762885623856	1.3952573275590847
9	0.6466229319890767	1.1715245101096738
>10	4.439752789876069	18.12213647776628
>50	0.9206480375704299	13.309442019247161
>100	0.7145097675104805	22.887709123840356
>500	0.014474423837638998	1.9313000104453908
>1k	0.007991088160363195	2.7717478697764117
>5k	0.0010554267381611769	1.628841978006473
>10k+	0.0010554267381611769	5.897218359727749
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTC	46101	1.3821237414627228	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCG	41382	1.240646508084649	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGC	35194	1.0551281524704252	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	32141	0.963598168680796	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCT	14942	0.44796626851773297	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	12840	0.3849475898653253	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	11694	0.35059011805958834	Illumina Single End Adapter 2 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTT	9907	0.2970152471024749	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCT	8956	0.2685039419652534	No Hit
GAACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCT	8647	0.2592400163212981	No Hit
ACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	7951	0.23837369836598143	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTC	7635	0.2288999103287974	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCACAGAT	5362	0.16075459321323007	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCC	5207	0.15610764022030754	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCACAGATCGTA	4895	0.14675377355068284	No Hit
AGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG	4585	0.13745986756483775	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3787	0.11353555473675911	No Hit
ACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG	3583	0.10741956499123524	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0986239322875992E-4	0.0	0.0	0.46799313690013455	0.0
2	2.0986239322875992E-4	0.0	0.0	2.491246489676719	0.0
3	2.0986239322875992E-4	0.0	0.0	3.2888734856554556	0.0
4	2.0986239322875992E-4	0.0	0.0	4.518996893736777	0.0
5	2.0986239322875992E-4	0.0	0.0	8.329888092377828	0.0
6	2.0986239322875992E-4	0.0	0.0	10.335393082755637	0.0
7	2.0986239322875992E-4	0.0	0.0	12.103963431178176	0.0
8	2.0986239322875992E-4	0.0	0.0	15.702443907529833	0.0
9	2.0986239322875992E-4	0.0	0.0	16.780117277101404	0.0
10	2.0986239322875992E-4	0.0	0.0	20.403061712435157	0.0
11	2.0986239322875992E-4	0.0	0.0	23.5429029185563	0.0
12	2.698230770084056E-4	0.0	0.0	26.93107129655085	0.0
13	2.698230770084056E-4	0.0	0.0	27.893470251556053	0.0
14	2.698230770084056E-4	0.0	0.0	28.251885238848885	0.0
15	2.698230770084056E-4	0.0	0.0	28.86564279801734	0.0
16	2.698230770084056E-4	0.0	0.0	30.088181179600536	0.0
17	2.698230770084056E-4	0.0	0.0	31.63057982880625	0.0
18	4.1972478645751983E-4	0.0	0.0	33.59989854652304	0.0
19	4.1972478645751983E-4	0.0	0.0	34.65880422207159	0.0
20	4.4970512834734263E-4	0.0	0.0	35.55377738816658	0.0
21	5.096658121269883E-4	0.0	0.0	36.639665371415965	0.0
22	5.096658121269883E-4	0.0	0.0	37.85701715385222	0.0
23	5.096658121269883E-4	0.0	0.0	39.03971166106384	0.0
24	5.096658121269883E-4	0.0	0.0	39.90995104509973	0.0
25	5.096658121269883E-4	0.0	0.0	40.58420893420185	0.0
26	5.096658121269883E-4	0.0	0.0	41.24818356603575	0.0
27	5.096658121269883E-4	0.0	0.0	41.86712772435114	0.0
28	5.396461540168112E-4	0.0	0.0	42.49404665360923	0.0
29	5.696264959066341E-4	0.0	0.0	43.163087963222516	0.0
30	5.996068377964569E-4	0.0	0.0	43.9334628284234	0.0
31	6.295871796862797E-4	0.0	0.0	44.63803084317613	0.0
32	6.295871796862797E-4	0.0	0.0	45.256405374995616	0.0
33	6.595675215761025E-4	0.0	0.0	45.85139524014104	0.0
34	6.595675215761025E-4	0.0	0.0	46.50976354804155	0.0
35	6.595675215761025E-4	0.0	0.0	47.16255551235055	0.0
36	6.595675215761025E-4	0.0	0.0	47.77394462450971	0.0
37	6.895478634659254E-4	0.0	0.0	48.35130604862392	0.0
38	6.895478634659254E-4	0.0	0.0	48.94254837103311	0.0
39	6.895478634659254E-4	0.0	0.0	49.4893298464197	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTAAG	120	0.0	45.000004	1
CGCCCAT	30	2.1667493E-6	45.000004	26
TAGACCG	20	7.03522E-4	45.0	1
GAGTACG	95	0.0	45.0	1
TTCGTAG	160	0.0	45.0	1
CACGAAA	25	3.8924074E-5	45.0	5
CGTTCGC	20	7.03522E-4	45.0	15
CCGCTAT	25	3.8924074E-5	45.0	30
CCTCCGT	20	7.03522E-4	45.0	26
TCGCTAG	25	3.8924074E-5	45.0	1
ACGGACG	45	3.8562575E-10	45.0	1
GTCCGCG	40	6.8212103E-9	45.0	1
CGTACTA	25	3.8924074E-5	45.0	36
CGTAAGA	20	7.03522E-4	45.0	31
ATCGGTG	25	3.8924074E-5	45.0	2
CGACTTG	25	3.8924074E-5	45.0	8
CAATCGT	20	7.03522E-4	45.0	15
TGTCGAC	40	6.8212103E-9	45.0	1
TCGCGGT	20	7.03522E-4	45.0	17
TCGCGAC	20	7.03522E-4	45.0	18
>>END_MODULE