FastQCFastQC Report
Sat 18 Jun 2016
SRR3548310_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548310_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2972094
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCG423641.4253923328131615No Hit
GAATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTC347931.1706561098000265No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGC314611.0585466004776432No Hit
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC196140.6599387502548708TruSeq Adapter, Index 21 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT80940.27233324383414526TruSeq Adapter, Index 14 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC79990.2691368442586271TruSeq Adapter, Index 21 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCT74540.25079960458854933No Hit
GAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT58360.1963598728707773No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTC57450.19329805854054416No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGATCGACT56930.19154845035183948No Hit
TCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC52040.1750954041157514TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTA41200.1386228026435234No Hit
ACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC34730.11685363921867882TruSeq Adapter, Index 14 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGT207.035093E-445.00000429
ACACCGT650.045.00000426
ATATGCG650.045.0000041
CGGTCGA207.035093E-445.00000413
GTTACGT253.8923044E-545.038
CGCAATA302.1666692E-644.99999643
ACGTAAG1100.042.9545481
CGGTCTA800.042.18750431
TATCGTG603.6379788E-1241.2499961
TACGGCT38200.040.464667
TCATCGA2700.040.00000416
GTTCACG403.460682E-739.3750041
CCAACGA403.460682E-739.37500419
CACGTTA403.460682E-739.37500413
CGAATAT2400.039.37499614
CATACGA8850.038.89830818
AACGCAT356.2516356E-638.57142640
CGTTAGG4150.038.4939772
GGGCGAT51050.038.256617
GGCGATA9150.038.1147548
Produced by FastQC (version 0.11.5)