##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548303_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	560922
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.932896195906025	28.0	25.0	31.0	16.0	33.0
2	26.618914929348467	30.0	25.0	31.0	16.0	33.0
3	24.633856400711686	28.0	16.0	31.0	10.0	33.0
4	30.47245962896802	33.0	28.0	35.0	19.0	37.0
5	32.25349157280335	35.0	32.0	35.0	28.0	37.0
6	32.077538409975006	35.0	32.0	35.0	25.0	37.0
7	33.11419234759913	35.0	32.0	35.0	28.0	37.0
8	33.35180649002891	35.0	33.0	36.0	28.0	37.0
9	35.224319958924774	37.0	35.0	39.0	30.0	39.0
10	35.636518089859194	37.0	35.0	39.0	30.0	39.0
11	35.684845664816145	37.0	35.0	39.0	30.0	39.0
12	34.62415629980639	35.0	33.0	39.0	27.0	39.0
13	34.33800599726878	37.0	33.0	39.0	26.0	39.0
14	35.34073186646272	37.0	33.0	40.0	27.0	41.0
15	35.94138578982461	38.0	34.0	40.0	30.0	41.0
16	35.74648346828971	37.0	34.0	40.0	30.0	41.0
17	35.548445238375386	37.0	34.0	40.0	30.0	41.0
18	35.467635428811846	37.0	34.0	39.0	29.0	40.0
19	34.768702243805734	36.0	33.0	39.0	27.0	40.0
20	34.70410146152228	35.0	33.0	39.0	28.0	40.0
21	35.11799679812879	35.0	34.0	39.0	30.0	40.0
22	35.34397117602804	36.0	34.0	39.0	30.0	40.0
23	35.509252623359394	36.0	34.0	40.0	30.0	41.0
24	35.42995817600308	36.0	34.0	40.0	30.0	41.0
25	34.731643615333326	35.0	33.0	39.0	27.0	40.0
26	34.69041150106432	35.0	33.0	39.0	27.0	40.0
27	34.97167698895747	35.0	34.0	39.0	29.0	41.0
28	34.85712451998673	36.0	34.0	40.0	27.0	41.0
29	34.770764919186625	36.0	34.0	40.0	27.0	41.0
30	34.421201165224396	36.0	33.0	40.0	25.0	41.0
31	34.21399588534591	35.0	33.0	39.0	24.0	41.0
32	33.7203907138604	35.0	33.0	40.0	22.0	41.0
33	33.40928150438029	35.0	33.0	40.0	20.0	41.0
34	33.216755627342124	35.0	33.0	40.0	17.0	41.0
35	32.97985103098114	35.0	33.0	40.0	15.0	41.0
36	32.62968291491509	35.0	32.0	40.0	12.0	41.0
37	32.63746652832301	35.0	32.0	40.0	12.0	41.0
38	32.41320183554933	35.0	31.0	40.0	10.0	41.0
39	32.358714758914786	35.0	31.0	40.0	10.0	41.0
40	32.15444036782298	35.0	31.0	40.0	10.0	41.0
41	32.02937663347132	35.0	31.0	39.0	10.0	41.0
42	32.10922730789664	35.0	31.0	39.0	10.0	41.0
43	32.08637208025358	35.0	31.0	40.0	10.0	41.0
44	32.016863307197795	35.0	31.0	39.0	10.0	40.0
45	31.95721151960522	35.0	31.0	39.0	10.0	40.0
46	31.796615215662783	35.0	31.0	39.0	10.0	40.0
47	31.78391113202905	35.0	31.0	39.0	10.0	40.0
48	31.722998919635884	35.0	31.0	39.0	10.0	40.0
49	31.710893849768773	35.0	31.0	39.0	10.0	40.0
50	31.551796863021952	35.0	30.0	39.0	10.0	40.0
51	29.916277129440456	34.0	26.0	37.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	39.0
9	66.0
10	85.0
11	64.0
12	54.0
13	50.0
14	81.0
15	150.0
16	339.0
17	656.0
18	1368.0
19	2284.0
20	3495.0
21	5181.0
22	7078.0
23	9339.0
24	12353.0
25	15460.0
26	17596.0
27	17405.0
28	16721.0
29	17764.0
30	21068.0
31	26805.0
32	36252.0
33	49101.0
34	63872.0
35	51974.0
36	52907.0
37	59194.0
38	54666.0
39	17452.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.53888775979548	19.792948039121303	23.04972883930386	23.618435361779355
2	33.844099536120886	28.086079704486544	26.33699516153761	11.73282559785496
3	25.136650015510178	23.05079850674425	38.21600864291292	13.59654283483265
4	21.46893864031006	22.297396072894273	43.67380847961036	12.559856807185312
5	26.107016661853162	25.60605574393588	34.89094740445196	13.395980189759005
6	17.393149136600098	40.079904157797344	33.46169342618047	9.065253279422095
7	69.36971628853922	3.743122929747808	25.12452711785239	1.7626336638605724
8	68.57210093381968	10.44316322055473	18.224637293598754	2.7600985520268417
9	65.65119570992046	4.127846652475745	18.201282887816845	12.019674749786958
10	45.060454038172864	21.338795768395606	23.851622863784982	9.749127329646546
11	40.08115210314447	16.09029419420169	31.139267135181004	12.689286567472841
12	33.855687600058474	16.28871750439455	33.02259494189923	16.832999953647747
13	19.564039206877247	31.06759941667469	36.72863606704676	12.639725309401307
14	14.865881530765417	36.13069196786719	32.45157080663621	16.551855694731174
15	10.997429232584922	19.43621394775031	55.304837392721275	14.261519426943497
16	13.025875255383102	18.112322212357512	46.5775990244633	22.284203507796093
17	13.515961220989729	19.073240129643693	38.40088283219414	29.009915817172445
18	16.38160029380199	22.153525802161443	40.84828193581282	20.616591968223748
19	21.248408869682418	22.180445766077995	34.53456986889443	22.036575495345165
20	24.43548300833271	20.4753958660919	39.05462791618086	16.034493209394533
21	18.070783460088926	27.93953526515273	36.24086771422765	17.7488135605307
22	18.534662573405928	19.854988750664084	37.07485176192055	24.535496914009432
23	17.068148512627424	28.516799127151373	33.97442068594208	20.440631674279132
24	17.794274426747393	21.31169752657232	41.546418218575845	19.347609828104442
25	13.896584551862826	28.670831238567928	38.759577980539184	18.67300622903006
26	14.016030749373353	27.788355600243886	35.7589825323307	22.436631118052063
27	16.62601930393174	30.939061045920823	34.39462171210971	18.04029793803773
28	12.423474208535232	27.225175692877084	44.83101037220861	15.520339726379067
29	15.80148398529564	23.28059872852197	38.86547505713807	22.052442229044324
30	14.035997874927354	28.413041385433267	38.18694934411558	19.364011395523796
31	24.526226462859363	25.43811795579421	30.950114276138212	19.085541305208213
32	27.070608747740327	25.87347260403407	31.669822185615825	15.386096462609775
33	22.204513283486833	24.109769272733107	31.66090829027922	22.024809153500843
34	20.152891132813476	26.91265452237566	33.20140768235156	19.73304666245931
35	18.996224073935412	23.08627581018395	31.767518478505032	26.149981637375607
36	26.38477364054182	27.77551959095917	29.103333440300077	16.73637332819893
37	19.50110710580081	26.488887938073386	34.64688495013567	19.363120005990137
38	18.644660041859655	30.800539112389956	28.394143927319664	22.16065691843073
39	23.753213459268846	26.614752140226273	31.98056057704993	17.651473823454957
40	19.813984832115693	24.221727798160885	32.21053907673438	23.753748292989044
41	17.462320964412164	25.120961559717752	32.400226769497365	25.016490706372725
42	19.40073664431062	24.71823176840987	30.29262535611012	25.588406231169397
43	25.293891129247914	24.963007334353083	27.24549937424455	22.497602162154454
44	18.57085298847255	27.795843272326632	31.27261900941664	22.36068472978418
45	16.634754921361615	31.586744681078656	27.736298451478103	24.04220194608163
46	26.47997404273678	28.92469897775448	27.38206025080136	17.213266728707378
47	18.746813282417165	25.052502843532615	36.065977087723425	20.1347067863268
48	22.074548689479105	24.955876218083798	30.77486709382053	22.194707998616565
49	19.577944883602356	21.84278741072734	35.62616549181526	22.953102213855043
50	21.071557186204142	26.858814594542558	31.114129950331776	20.955498268921527
51	18.533236350152073	27.057594460548884	26.84169991549627	27.567469273802775
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	21.0
1	252.5
2	484.0
3	5607.5
4	10731.0
5	10000.0
6	9269.0
7	9167.0
8	9065.0
9	9135.0
10	9205.0
11	8866.0
12	8527.0
13	8100.5
14	7674.0
15	7123.5
16	6573.0
17	6054.0
18	5535.0
19	5088.5
20	4642.0
21	4267.5
22	3893.0
23	3873.5
24	3854.0
25	4197.5
26	4985.5
27	5430.0
28	5877.0
29	6324.0
30	7276.5
31	8229.0
32	9225.0
33	10221.0
34	11667.0
35	13113.0
36	14087.0
37	15061.0
38	16940.5
39	18820.0
40	24539.0
41	30258.0
42	35598.5
43	40939.0
44	41979.5
45	43020.0
46	64369.0
47	85718.0
48	63470.5
49	41223.0
50	37534.5
51	33846.0
52	29993.0
53	26140.0
54	24298.0
55	22456.0
56	20059.0
57	17662.0
58	16515.0
59	15368.0
60	15139.5
61	14911.0
62	12341.0
63	9771.0
64	8341.5
65	6912.0
66	5572.0
67	4232.0
68	3431.5
69	2631.0
70	2125.0
71	1619.0
72	1374.5
73	1130.0
74	899.5
75	618.0
76	567.0
77	449.5
78	332.0
79	236.5
80	141.0
81	97.5
82	54.0
83	46.0
84	38.0
85	27.5
86	17.0
87	16.0
88	15.0
89	15.5
90	16.0
91	20.0
92	24.0
93	12.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	560922.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.232026815181584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.24969682372121	26.83306460377918
2	6.820965238944149	4.397070689741384
3	2.3859662431443205	2.3071358378743736
4	1.143640586808118	1.4744741624371702
5	0.6934507613297685	1.117566176709459
6	0.49327561245330953	0.9539563660722113
7	0.37120551468392143	0.8375294272264805
8	0.2863137796543321	0.7382778738699538
9	0.2349229248795877	0.6814837812797783
>10	3.326132813314357	26.366692193873604
>50	0.8440959836603812	18.770624239745906
>100	0.13975467803348	6.187601179229381
>500	0.0033407492757007167	0.6051566557329737
>1k	0.005567915459501195	3.368023415818718
>5k	0.001113583091900239	3.0225529050577533
>10k+	5.567915459501195E-4	2.3387904915516353
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGC	13032	2.323317680533122	No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTC	8446	1.5057352002595727	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCG	8396	1.4968213049229662	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4291	0.7649904977875712	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTC	1906	0.33979769023144035	No Hit
GAACTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCT	1849	0.32963584954770897	No Hit
GATTGATACGGCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGC	1827	0.32571373559960204	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCT	1672	0.2980806600561219	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACAGTTCGTCGTA	1627	0.290058154253176	No Hit
GCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGC	1596	0.28453153914448	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACAGTTCGT	1370	0.24424073222301854	No Hit
GATTCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTC	1348	0.24031861827491166	No Hit
GATTGATACCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCG	1281	0.22837399852385895	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACAGTTCGTCGT	621	0.11071058008065293	No Hit
GTATGATACGGCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGC	597	0.10643191031908181	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	568	0.10126185102385002	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10037046149018937	0.0
2	0.0	0.0	0.0	0.5494525085484256	0.0
3	0.0	0.0	0.0	0.6303906782048128	0.0
4	0.0	0.0	0.0	1.1853698018619345	0.0
5	0.0	0.0	0.0	3.4537778871215608	0.0
6	0.0	0.0	0.0	3.673237990308813	0.0
7	0.0	0.0	0.0	4.169028848930868	0.0
8	0.0	0.0	0.0	4.819921486409875	0.0
9	0.0	0.0	0.0	4.936871793226152	0.0
10	0.0	0.0	0.0	7.062122719379878	0.0
11	0.0	0.0	0.0	7.273203760950721	0.0
12	0.0	0.0	0.0	10.49504209141378	0.0
13	0.0	0.0	0.0	10.8180816584124	0.0
14	0.0	0.0	0.0	11.006877961641726	0.0
15	0.0	0.0	0.0	11.486267252844424	0.0
16	0.0	0.0	0.0	11.769729124548512	0.0
17	0.0	0.0	0.0	11.929644406887233	0.0
18	1.782779067321303E-4	0.0	0.0	12.145538951939843	0.0
19	1.782779067321303E-4	0.0	0.0	12.593729609464418	0.0
20	1.782779067321303E-4	0.0	0.0	12.725476982539462	0.0
21	1.782779067321303E-4	0.0	0.0	12.80980243242376	0.0
22	1.782779067321303E-4	0.0	0.0	12.93620146829684	0.0
23	1.782779067321303E-4	0.0	0.0	13.021061751901334	0.0
24	1.782779067321303E-4	0.0	0.0	13.086133187858561	0.0
25	1.782779067321303E-4	0.0	0.0	13.138546892437807	0.0
26	1.782779067321303E-4	0.0	0.0	13.208788387690268	0.0
27	1.782779067321303E-4	0.0	0.0	13.290261391066851	0.0
28	1.782779067321303E-4	0.0	0.0	13.343923040993221	0.0
29	1.782779067321303E-4	0.0	0.0	13.404715807188879	0.0
30	1.782779067321303E-4	0.0	0.0	13.482801530337552	0.0
31	1.782779067321303E-4	0.0	0.0	13.547338132574582	0.0
32	1.782779067321303E-4	0.0	0.0	13.604565340635597	0.0
33	1.782779067321303E-4	0.0	0.0	13.670706444033216	0.0
34	1.782779067321303E-4	0.0	0.0	13.744335219513587	0.0
35	1.782779067321303E-4	0.0	0.0	13.824381999636312	0.0
36	1.782779067321303E-4	0.0	0.0	13.896762829769559	0.0
37	1.782779067321303E-4	0.0	0.0	13.965756379674893	0.0
38	1.782779067321303E-4	0.0	0.0	14.03171920516578	0.0
39	1.782779067321303E-4	0.0	0.0	14.105526258552883	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	30	2.163506E-6	45.000004	21
ACCGGTC	30	2.163506E-6	45.000004	17
CGGTTAT	60	0.0	45.000004	31
GAGTAAG	60	0.0	45.000004	1
AGACGTG	30	2.163506E-6	45.000004	24
TACGACG	65	0.0	45.000004	1
AGACACG	30	2.163506E-6	45.000004	24
TCCCGCA	30	2.163506E-6	45.000004	31
GCGTATG	30	2.163506E-6	45.000004	1
CTTGTTC	20	7.030195E-4	45.0	20
TTTAGTG	20	7.030195E-4	45.0	1
TGTCACG	20	7.030195E-4	45.0	1
AAGTACG	20	7.030195E-4	45.0	11
TCCATTG	25	3.8882426E-5	45.0	1
GTACCGG	25	3.8882426E-5	45.0	2
CGAAAAG	25	3.8882426E-5	45.0	44
ACGTTAG	35	1.2104465E-7	45.0	1
GCGAACG	20	7.030195E-4	45.0	10
GCGCGAC	35	1.2104465E-7	45.0	9
ATAATGC	35	1.2104465E-7	45.0	40
>>END_MODULE