##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548302_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	702523
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.85646590930119	28.0	25.0	31.0	16.0	33.0
2	26.55765576358354	30.0	25.0	31.0	16.0	33.0
3	24.6929879875819	28.0	16.0	31.0	10.0	33.0
4	30.528591946455844	33.0	28.0	35.0	19.0	37.0
5	32.21997429265661	35.0	32.0	35.0	26.0	37.0
6	31.986064513190314	35.0	32.0	35.0	25.0	37.0
7	32.92432133894549	35.0	32.0	35.0	28.0	37.0
8	33.22436703139968	35.0	33.0	36.0	28.0	37.0
9	35.092654617713585	37.0	35.0	39.0	29.0	39.0
10	35.49311410444925	37.0	35.0	39.0	30.0	39.0
11	35.57285526594859	37.0	35.0	39.0	30.0	39.0
12	34.35718830557861	35.0	33.0	39.0	27.0	39.0
13	33.95251828054028	35.0	33.0	39.0	25.0	39.0
14	34.86177249712821	37.0	33.0	40.0	25.0	41.0
15	35.59987786876729	37.0	33.0	40.0	30.0	41.0
16	35.40649914664716	36.0	34.0	40.0	30.0	41.0
17	35.28390387218639	36.0	34.0	39.0	29.0	41.0
18	35.14745709393144	36.0	34.0	39.0	29.0	40.0
19	34.47093404771089	36.0	33.0	39.0	27.0	40.0
20	34.50798763883887	35.0	33.0	39.0	27.0	40.0
21	34.86425070780601	35.0	34.0	39.0	29.0	40.0
22	35.083301187292086	35.0	33.0	39.0	29.0	40.0
23	35.30834150625673	35.0	34.0	39.0	30.0	41.0
24	35.17703192635686	35.0	34.0	39.0	30.0	41.0
25	34.545624840752545	35.0	33.0	39.0	27.0	40.0
26	34.470975327498174	35.0	33.0	39.0	27.0	40.0
27	34.7217130257657	35.0	33.0	39.0	27.0	40.0
28	34.582964543509604	35.0	33.0	39.0	27.0	41.0
29	34.596668009445956	35.0	33.0	39.0	27.0	41.0
30	34.25979362953241	35.0	33.0	39.0	25.0	41.0
31	34.09888217182925	35.0	33.0	39.0	24.0	40.0
32	33.55551206152681	35.0	33.0	39.0	23.0	40.0
33	33.38388494042188	35.0	33.0	39.0	21.0	41.0
34	33.31024464679448	35.0	33.0	40.0	18.0	41.0
35	33.07357339190318	35.0	33.0	40.0	17.0	41.0
36	32.83032583986574	35.0	33.0	40.0	15.0	41.0
37	32.61096077993176	35.0	32.0	39.0	15.0	41.0
38	32.560418662449486	35.0	32.0	39.0	15.0	41.0
39	32.35606521067638	35.0	31.0	39.0	15.0	40.0
40	32.2386356033895	35.0	31.0	39.0	14.0	40.0
41	32.18759101125515	35.0	31.0	39.0	13.0	40.0
42	32.21226778340353	35.0	31.0	39.0	12.0	40.0
43	32.175394969275025	35.0	31.0	39.0	12.0	40.0
44	32.15453444228872	35.0	31.0	39.0	12.0	40.0
45	32.07046886721147	35.0	31.0	39.0	12.0	40.0
46	31.94734264927981	35.0	31.0	39.0	11.0	40.0
47	31.990975384435814	35.0	31.0	39.0	12.0	40.0
48	31.89304976491873	35.0	31.0	38.0	11.0	40.0
49	31.898508660926403	35.0	31.0	38.0	10.0	40.0
50	31.69916002750088	35.0	31.0	38.0	10.0	40.0
51	30.02574862317675	34.0	27.0	37.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	42.0
9	74.0
10	110.0
11	81.0
12	71.0
13	91.0
14	128.0
15	233.0
16	452.0
17	928.0
18	1767.0
19	2887.0
20	4362.0
21	6091.0
22	8291.0
23	10937.0
24	14441.0
25	18079.0
26	21040.0
27	21045.0
28	20983.0
29	23435.0
30	28495.0
31	37139.0
32	49570.0
33	67228.0
34	87218.0
35	66401.0
36	62995.0
37	68296.0
38	60748.0
39	18860.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.00287250381838	20.165460774949718	23.603497679079545	22.228169042152356
2	32.54057162541298	27.48408237168036	28.24918187731932	11.726164125587347
3	26.13764958584986	22.372505953541737	37.30966815321349	14.180176307394918
4	22.24767018304027	22.515846456272605	41.70980878917843	13.52667457150869
5	26.318853617603978	26.440273129847707	32.78597284359373	14.454900408954583
6	18.691772369018523	39.79271853021182	31.91311885874199	9.602390242027663
7	72.04290820371718	4.009263753642229	21.9935859751211	1.9542420675194974
8	71.26215084773025	10.135041842046451	15.782686118461603	2.820121191761693
9	68.38523436243369	4.098655844719675	16.03648563819263	11.479624154654012
10	49.82356449539731	17.6910933876898	21.20400328530169	11.281338831611206
11	45.13418066027731	16.360887828583547	25.95416804859058	12.550763462548556
12	38.17540493336161	17.320856399007578	28.98310802635643	15.520630641274376
13	20.475913244121543	34.152903178970654	32.81415697422006	12.55702660268774
14	15.663117079440816	38.13825312480873	30.463344260614956	15.735285535135505
15	12.265790586215683	19.603201603363875	54.00691507609003	14.124092734330407
16	13.591156446123472	17.715149539588026	46.19322072017571	22.50047329411279
17	14.128078368964433	18.397404782476872	35.858043081863514	31.616473766695186
18	18.518112574250235	20.822094080905536	38.77325012846555	21.88654321637868
19	23.738866912542363	22.026040428569598	31.54103139683683	22.694061262051207
20	26.818196699609835	20.14880651594325	36.1125543220649	16.920442462382017
21	21.099522720252576	27.886489125622933	33.262825558736154	17.751162595388337
22	21.233183824586526	20.062545994935395	33.893267551382664	24.81100262909542
23	19.41032535589582	28.939977765852504	30.073606131044823	21.57609074720685
24	19.416161463752786	20.942801872678903	39.59272507804015	20.04831158552816
25	14.803643439431877	27.539881256556725	37.96843662058039	19.688038683431007
26	15.294445875793391	27.835956972227244	33.34894373564994	23.52065341632943
27	18.887353154273953	30.18733906220864	31.943153462591262	18.98215432092615
28	14.009790426790298	25.83758823554531	43.893509536342584	16.259111801321808
29	16.68856393313813	21.001874671718934	39.65749164084308	22.65206975429986
30	18.27513120566871	28.347683990417394	35.697905976032104	17.67927882788179
31	27.956807108094683	23.704989018153142	27.41447610967897	20.923727764073206
32	28.529742086735947	25.198178564972253	30.293670100480696	15.978409247811104
33	24.22312152057655	23.88220741527324	29.444872267527185	22.44979879662303
34	20.460113049679514	24.04006701559949	32.713804387899046	22.786015546821954
35	22.91028194094713	22.29507076636637	31.925787483114433	22.86885980957207
36	28.4027711548234	20.976679767068124	29.06837213870578	21.552176939402695
37	22.152584328199932	25.13668591633299	34.900352016944645	17.81037773852244
38	23.20166030151326	27.577175409203686	27.164946912770116	22.05621737651294
39	20.13599554747674	26.73293258726049	33.5628869090407	19.568184956222073
40	23.097891456934505	22.936330910162372	30.539783039131812	23.425994593771307
41	17.16797884197386	22.90857381181826	32.64818376053169	27.275263585676196
42	22.82530251678593	23.26372232652881	29.94179549993381	23.969179656751454
43	21.99671754519069	24.2568570708717	29.567145844335347	24.179279539602263
44	20.78565399282301	27.489918479537323	30.599709902736283	21.124717624903383
45	17.65693080511243	31.682948458626974	25.86192907563169	24.798191660628905
46	25.075050923599658	30.232462140029583	26.819050764174268	17.873436172196495
47	19.88760510332046	25.291413946589646	35.17863472085612	19.642346229233777
48	22.58402927733327	24.099993879203954	30.2271954085489	23.088781434913873
49	19.997494743944326	21.470756117593304	36.298598053017486	22.233151085444888
50	21.061943879417473	26.730228049473116	30.204135665309177	22.003692405800237
51	18.99738513899189	28.72660396883803	25.161169100513437	27.11484179165664
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	101.0
1	270.0
2	439.0
3	5369.5
4	10300.0
5	9538.0
6	8776.0
7	8707.0
8	8638.0
9	9008.0
10	9378.0
11	9212.0
12	9046.0
13	8618.0
14	8190.0
15	7513.0
16	6836.0
17	6318.5
18	5801.0
19	5260.5
20	4720.0
21	4370.0
22	4020.0
23	4201.5
24	4383.0
25	4186.5
26	4419.5
27	4849.0
28	5244.5
29	5640.0
30	6421.5
31	7203.0
32	8649.0
33	10095.0
34	11446.5
35	12798.0
36	13438.5
37	14079.0
38	16583.5
39	19088.0
40	29033.5
41	38979.0
42	45845.0
43	52711.0
44	56943.5
45	61176.0
46	82915.5
47	104655.0
48	80216.5
49	55778.0
50	50419.0
51	45060.0
52	42794.5
53	40529.0
54	35896.5
55	31264.0
56	28433.5
57	25603.0
58	23615.0
59	21627.0
60	20142.5
61	18658.0
62	16696.0
63	14734.0
64	12740.5
65	10747.0
66	9279.5
67	7812.0
68	6462.5
69	5113.0
70	4177.0
71	3241.0
72	2932.5
73	2624.0
74	2158.0
75	1363.0
76	1034.0
77	764.0
78	494.0
79	386.0
80	278.0
81	210.0
82	142.0
83	103.0
84	64.0
85	50.5
86	37.0
87	43.5
88	50.0
89	34.0
90	18.0
91	17.0
92	16.0
93	14.5
94	13.0
95	7.5
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	702523.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.378959979687647
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.46176183342826	23.40179038925395
2	7.286319709821891	4.135563508884895
3	2.40849022679282	2.050513432728668
4	1.2636699110680523	1.4344655133494282
5	0.760691827941798	1.0793821471017864
6	0.5427478399065448	0.9241571536661852
7	0.40647255699790485	0.8074687899519394
8	0.3110741772469413	0.7062369301444166
9	0.23939446097033176	0.6114389244512347
>10	2.81389515365612	19.21114229976986
>50	1.1155695402701045	22.626618849182897
>100	0.3796922438940362	14.580841467220129
>500	0.003066155401566915	0.5479000513537527
>1k	0.005621284902872677	3.120042801700539
>5k	5.110259002611524E-4	1.277079897358694
>10k+	0.0010220518005223049	3.485357843881614
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGC	13017	1.852893072539974	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCG	11016	1.5680625403011719	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTC	8806	1.2534820924012453	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4178	0.5947136250343406	No Hit
GAACTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCT	2195	0.31244528648884096	No Hit
GCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	2035	0.28967023143726256	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTC	1956	0.27842504800554574	No Hit
GATTGATACGGCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGC	1896	0.2698844023612038	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCT	1785	0.2540842079191713	No Hit
GATTGATACCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCG	1630	0.2320208733379548	No Hit
GATTCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTC	1558	0.2217720985647445	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTA	1545	0.21992162534180373	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGGTTTGTT	1379	0.19629250572579118	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1357	0.19316093565619916	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	935	0.13309172795766117	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07586940214057049	0.0
2	0.0	0.0	0.0	0.47500224191948165	0.0
3	0.0	0.0	0.0	0.5345020732417302	0.0
4	0.0	0.0	0.0	1.0213188749692181	0.0
5	0.0	0.0	0.0	2.9263098859396774	0.0
6	0.0	0.0	0.0	3.1069445413175085	0.0
7	0.0	0.0	0.0	3.5258632101724783	0.0
8	0.0	0.0	0.0	4.05324807871059	0.0
9	0.0	0.0	0.0	4.162710687052239	0.0
10	0.0	0.0	0.0	6.340005949983132	0.0
11	0.0	0.0	0.0	6.485908646407307	0.0
12	0.0	0.0	0.0	9.11471937573574	0.0
13	0.0	0.0	0.0	9.368234207278624	0.0
14	0.0	0.0	0.0	9.517126129678317	0.0
15	0.0	0.0	0.0	9.89462266715823	0.0
16	0.0	0.0	0.0	10.119526335792564	0.0
17	0.0	0.0	0.0	10.233828643332673	0.0
18	5.693763762894596E-4	0.0	0.0	10.385140415331598	1.423440940723649E-4
19	5.693763762894596E-4	0.0	0.0	10.752815210320517	1.423440940723649E-4
20	5.693763762894596E-4	0.0	0.0	10.858719216310355	1.423440940723649E-4
21	5.693763762894596E-4	0.0	0.0	10.907827928765322	1.423440940723649E-4
22	5.693763762894596E-4	0.0	0.0	11.003198471793805	1.423440940723649E-4
23	5.693763762894596E-4	0.0	0.0	11.055723442506508	1.423440940723649E-4
24	5.693763762894596E-4	0.0	0.0	11.108960133689573	1.423440940723649E-4
25	5.693763762894596E-4	0.0	0.0	11.149812888688341	1.423440940723649E-4
26	5.693763762894596E-4	0.0	0.0	11.197071127920367	1.423440940723649E-4
27	5.693763762894596E-4	0.0	0.0	11.266392701733608	1.423440940723649E-4
28	5.693763762894596E-4	0.0	0.0	11.302121069345771	1.423440940723649E-4
29	5.693763762894596E-4	0.0	0.0	11.34710180307264	1.423440940723649E-4
30	5.693763762894596E-4	0.0	0.0	11.395641139151316	1.423440940723649E-4
31	5.693763762894596E-4	0.0	0.0	11.44218765791298	1.423440940723649E-4
32	5.693763762894596E-4	0.0	0.0	11.488591832580571	1.423440940723649E-4
33	5.693763762894596E-4	0.0	0.0	11.529871619861556	1.423440940723649E-4
34	5.693763762894596E-4	0.0	0.0	11.589940827560094	1.423440940723649E-4
35	5.693763762894596E-4	0.0	0.0	11.642323454178724	1.423440940723649E-4
36	5.693763762894596E-4	0.0	0.0	11.68716184381152	1.423440940723649E-4
37	5.693763762894596E-4	0.0	0.0	11.733423674385039	1.423440940723649E-4
38	5.693763762894596E-4	0.0	0.0	11.777265655359326	1.423440940723649E-4
39	5.693763762894596E-4	0.0	0.0	11.821534668615831	1.423440940723649E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	30	2.1642918E-6	45.000004	6
CTGTCGA	20	7.031411E-4	45.000004	13
ATCTCGC	20	7.031411E-4	45.000004	38
GTCGCCC	20	7.031411E-4	45.000004	13
GGGTCTA	40	6.8084773E-9	45.000004	7
CGGCCTA	20	7.031411E-4	45.000004	45
CCGGCAC	20	7.031411E-4	45.000004	36
ATGTGGA	30	2.1642918E-6	45.000004	12
GCTGCGT	30	2.1642918E-6	45.000004	38
CGTCTAG	20	7.031411E-4	45.000004	30
GATCAAT	25	3.8892507E-5	45.000004	27
GAATACC	20	7.031411E-4	45.000004	27
CGACAAA	20	7.031411E-4	45.000004	43
TATGCGT	30	2.1642918E-6	45.000004	20
CGTTCGG	30	2.1642918E-6	45.000004	31
CGTTCCC	25	3.8892507E-5	45.000004	18
CTTGGTA	25	3.8892507E-5	45.000004	40
CATTAGT	25	3.8892507E-5	45.000004	25
ATGTAGT	20	7.031411E-4	45.000004	39
ATGTACT	20	7.031411E-4	45.000004	20
>>END_MODULE