SRR3548298_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548298_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	745393
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGC	13476	1.8079053599913064	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCG	12262	1.6450382549876372	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTC	10770	1.4448753878826337	RNA PCR Primer, Index 27 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6267	0.8407645362915939	No Hit
GCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	3653	0.4900770466049453	Illumina PCR Primer Index 6 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT	3130	0.41991271718408946	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTC	2704	0.3627616572734115	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCT	2418	0.3243926358310314	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCCTATTT	1807	0.24242245365867404	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTA	1689	0.22659187837825145	No Hit
TGTGAGGGGATCTGTACGGGACGACATCATCCAAAACGATCTTTTTCTGTA	1318	0.17681947643726195	No Hit
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	1203	0.1613913734097315	RNA PCR Primer, Index 27 (96% over 25bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	798	0.10705761926929822	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAT	35	1.2111559E-7	45.000004	30
CGGGTAG	35	1.2111559E-7	45.000004	6
GAATCCC	35	1.2111559E-7	45.000004	12
GGATCGA	65	0.0	45.000004	9
GTTAGCG	35	1.2111559E-7	45.000004	1
CGTTCTA	35	1.2111559E-7	45.000004	29
TCTAGCA	35	1.2111559E-7	45.000004	35
AGTCTAC	35	1.2111559E-7	45.000004	38
GTCCGGA	35	1.2111559E-7	45.000004	27
CGTACCT	35	1.2111559E-7	45.000004	36
ACCGTAG	35	1.2111559E-7	45.000004	1
GTCCAAT	35	1.2111559E-7	45.000004	41
TATGTAG	130	0.0	45.000004	1
TACGTAG	35	1.2111559E-7	45.000004	1
TGATCGA	20	7.03169E-4	45.0	34
TCGCCCG	20	7.03169E-4	45.0	37
AGCGTCG	25	3.889481E-5	45.0	25
TCGTTGC	20	7.03169E-4	45.0	44
TCGTTCC	25	3.889481E-5	45.0	13
GTCGCCC	25	3.889481E-5	45.0	12