##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548298_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	745393
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.749450289981258	28.0	25.0	31.0	16.0	33.0
2	27.595692473634713	30.0	25.0	31.0	16.0	33.0
3	27.525812557939236	30.0	25.0	31.0	16.0	33.0
4	30.352553619365892	33.0	28.0	35.0	19.0	37.0
5	31.684387967152897	33.0	30.0	35.0	25.0	37.0
6	30.885951437697965	33.0	30.0	35.0	17.0	37.0
7	31.65123364453382	33.0	30.0	35.0	25.0	35.0
8	33.04662775207173	35.0	32.0	35.0	28.0	37.0
9	34.19054780498341	35.0	32.0	37.0	27.0	39.0
10	33.61957249397298	35.0	32.0	38.0	27.0	39.0
11	33.73270476111259	37.0	32.0	39.0	25.0	39.0
12	33.16936434873952	35.0	31.0	37.0	23.0	39.0
13	33.294115989820135	35.0	32.0	38.0	23.0	39.0
14	33.9976670025074	36.0	32.0	39.0	23.0	40.0
15	35.113117509823674	37.0	33.0	39.0	27.0	41.0
16	35.16220570893475	37.0	33.0	39.0	27.0	41.0
17	34.72345997346366	36.0	33.0	39.0	27.0	40.0
18	34.612796202808454	36.0	33.0	39.0	27.0	40.0
19	33.93885641534063	36.0	32.0	38.0	25.0	40.0
20	33.88262970003743	35.0	32.0	38.0	27.0	40.0
21	34.19898094025568	35.0	33.0	38.0	27.0	40.0
22	34.456191566059786	35.0	33.0	39.0	27.0	40.0
23	34.89141835246642	35.0	33.0	39.0	28.0	40.0
24	34.4937261283645	35.0	33.0	39.0	27.0	40.0
25	33.71970893206671	35.0	32.0	39.0	24.0	40.0
26	33.60762711750714	35.0	32.0	39.0	23.0	40.0
27	34.112838462395004	35.0	33.0	39.0	25.0	40.0
28	34.02028191839741	35.0	33.0	39.0	24.0	40.0
29	34.04379568898554	35.0	33.0	39.0	24.0	40.0
30	33.53911963219402	35.0	32.0	39.0	23.0	40.0
31	33.52133035861619	35.0	33.0	39.0	23.0	40.0
32	32.95484261322551	35.0	31.0	39.0	19.0	40.0
33	32.77300162464633	35.0	31.0	39.0	17.0	40.0
34	32.48899842096719	35.0	31.0	39.0	15.0	40.0
35	32.4064245304155	35.0	31.0	39.0	12.0	40.0
36	32.070011389964755	35.0	31.0	39.0	10.0	40.0
37	32.075616486873365	35.0	31.0	39.0	10.0	40.0
38	31.966492843372556	35.0	31.0	39.0	10.0	40.0
39	31.744242299028834	35.0	30.0	39.0	10.0	40.0
40	31.561773453735142	35.0	30.0	39.0	10.0	40.0
41	31.253204685313655	35.0	29.0	39.0	10.0	40.0
42	31.539031088298387	35.0	30.0	39.0	10.0	40.0
43	31.47010234869391	35.0	30.0	39.0	10.0	40.0
44	31.40136008789994	35.0	30.0	39.0	10.0	40.0
45	31.442645691601612	35.0	30.0	39.0	10.0	40.0
46	31.20337996197979	35.0	29.0	39.0	10.0	40.0
47	31.29185543733306	35.0	30.0	39.0	10.0	40.0
48	31.138214337939853	35.0	29.0	38.0	10.0	40.0
49	31.248510517270756	35.0	30.0	38.0	10.0	40.0
50	31.160518008620954	35.0	29.0	39.0	10.0	40.0
51	29.28029106793329	33.0	24.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	21.0
9	50.0
10	60.0
11	42.0
12	53.0
13	83.0
14	109.0
15	306.0
16	683.0
17	1432.0
18	2662.0
19	4283.0
20	6379.0
21	8909.0
22	11606.0
23	15188.0
24	19336.0
25	23297.0
26	25246.0
27	25403.0
28	25395.0
29	27990.0
30	33891.0
31	42399.0
32	53516.0
33	66296.0
34	72352.0
35	69665.0
36	78200.0
37	77636.0
38	47950.0
39	4952.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.71549370600475	20.04016673083863	23.025705902792218	24.218633660364397
2	36.2532248089263	27.16727954246954	24.850515097404994	11.728980551199166
3	24.51458492365772	26.345833674316772	36.91716986878063	12.222411533244879
4	24.690062825918677	21.709889950670316	39.98199607455396	13.618051148857047
5	24.777399304796262	26.964165212176667	35.000328685673196	13.25810679735388
6	17.309124180130482	38.199178151659595	34.66802076220195	9.823676906007972
7	68.87132022973115	4.300952651822596	24.307580028253554	2.5201470901926903
8	67.15356865438768	9.682945774913367	19.32282701876728	3.840658551931666
9	62.70692104701815	5.84188475072881	20.591419559883175	10.859774642369864
10	41.02131358893899	21.411658011277275	26.039015660195357	11.528012739588378
11	35.764221021662394	19.640109311463885	31.105470537018725	13.490199129854991
12	30.554888495062336	18.412971412395876	34.451624847563636	16.58051524497815
13	18.529956680569846	29.940179207478472	38.09654772717211	13.433316384779573
14	15.734384411981331	33.6347403316103	34.54620582699327	16.084669429415086
15	12.896686714256775	20.63139847033712	52.99754626083153	13.474368554574568
16	13.127169157746316	21.05681164164407	45.00699630932944	20.809022891280172
17	13.316196959187973	21.101083589462203	38.883783453829054	26.698935997520774
18	17.271291788358624	22.40482537399734	40.781842598468195	19.542040239175844
19	20.68640301156571	23.878812921505837	35.86309503845622	19.57168902847223
20	23.782622053064625	21.99027895351848	38.79215393758729	15.43494505582961
21	19.168545988491978	26.22200637784363	37.318971334584575	17.290476299079817
22	17.25908346335423	21.89623460375936	36.336939037527856	24.507742895358557
23	17.00176953633855	28.28024947913383	35.083506284604226	19.634474699923395
24	18.558398053107556	22.015768862868313	39.746683964029714	19.679149119994417
25	15.07070766696226	26.973958703663705	37.86043067214207	20.09490295723196
26	14.954795658129335	27.744558910534444	36.36283141913058	20.937814012205642
27	17.294232706773474	28.85994368071608	35.10725214752487	18.738571464985583
28	13.923259273966886	25.234473626664055	42.49127641391857	18.350990685450494
29	17.56375495879355	22.729754639498896	38.46628557016232	21.24020483154524
30	15.819037742506303	26.068798606909375	38.21111816182873	19.901045488755596
31	23.16603456163393	24.29738406451362	30.978825934775344	21.557755439077102
32	23.423616803484872	26.009501028316606	31.96770026013123	18.59918190806729
33	18.234407889529418	25.407939167660547	32.11487094727211	24.242781995537925
34	20.413392666687237	27.21396632380503	31.94368608237534	20.4289549271324
35	17.710254858846273	23.785975988505392	32.882653848372605	25.621115304275733
36	24.234464235644822	23.644305755487373	31.73493714054197	20.386292868325835
37	17.6784595508678	26.8585833245013	33.666938111841674	21.796019012789227
38	17.909478624027862	27.363283529627996	30.142354435847935	24.584883410496207
39	19.652049321633015	26.798749116238014	31.473061861326844	22.076139700802127
40	20.364961838922554	23.136385772337544	34.86013418424911	21.638518204490786
41	16.913359798119917	26.655737309043687	32.13298219865226	24.297920694184143
42	21.035883084493683	23.36914889192681	33.79854653853739	21.79642148504212
43	21.964654886751017	24.637338960789812	31.048990264196203	22.349015888262972
44	19.28392136765438	26.676800023611708	31.30120620934192	22.738072399392
45	16.662217112315247	29.68018213211018	29.216668254196108	24.44093250137847
46	22.868205094493778	28.43842107452042	29.26858717481919	19.424786656166614
47	18.203417526056725	25.77056666751633	35.5230059847624	20.503009821664545
48	21.511471129994515	24.427248444780137	31.81516327628513	22.246117148940222
49	18.33676999918164	22.044881022494174	36.546224609031746	23.07212436929244
50	19.3115577956863	25.51419184242406	33.06416883442694	22.110081527462693
51	17.47990657277436	26.159489021227728	29.443796762244883	26.916807643753028
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	69.0
1	235.5
2	402.0
3	8109.5
4	15817.0
5	13452.5
6	11088.0
7	11357.5
8	11627.0
9	11981.5
10	12336.0
11	12084.5
12	11833.0
13	11205.5
14	10578.0
15	10101.0
16	9624.0
17	9048.5
18	8473.0
19	7840.5
20	7208.0
21	7032.0
22	6856.0
23	6601.0
24	6346.0
25	6063.5
26	6762.0
27	7743.0
28	8010.5
29	8278.0
30	9426.5
31	10575.0
32	12551.0
33	14527.0
34	15462.0
35	16397.0
36	17946.0
37	19495.0
38	20445.5
39	21396.0
40	29356.0
41	37316.0
42	45540.5
43	53765.0
44	56325.0
45	58885.0
46	83125.0
47	107365.0
48	83836.0
49	60307.0
50	56104.0
51	51901.0
52	43227.0
53	34553.0
54	31180.5
55	27808.0
56	26338.0
57	24868.0
58	23695.0
59	22522.0
60	21396.5
61	20271.0
62	15784.5
63	11298.0
64	9235.0
65	7172.0
66	6128.5
67	5085.0
68	3912.0
69	2739.0
70	2075.5
71	1412.0
72	1133.5
73	855.0
74	642.5
75	340.0
76	250.0
77	161.0
78	72.0
79	50.5
80	29.0
81	24.0
82	19.0
83	13.0
84	7.0
85	6.5
86	6.0
87	6.5
88	7.0
89	4.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	745393.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.386249202261723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.9973914974315	26.677493110805873
2	7.621751250796674	4.784363682303088
3	2.350965217295985	2.2136394052770356
4	1.0469231725970194	1.3143596636301005
5	0.5907524762330568	0.9270752217951961
6	0.3799553521712479	0.715522402138794
7	0.2603101305861428	0.5719111039914978
8	0.18856074382928506	0.47345715964718205
9	0.13968429836750562	0.3945749578385051
>10	1.234910604181174	8.73566965557631
>50	0.5041259414993605	11.70874059762025
>100	0.6781278884344936	32.874783070175425
>500	0.0013082853153077046	0.30057507659620053
>1k	0.0034887608408205456	2.4530539721116145
>5k	4.360951051025682E-4	0.857788709029321
>10k+	0.0013082853153077046	4.996992211463611
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGC	13476	1.8079053599913064	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCG	12262	1.6450382549876372	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTC	10770	1.4448753878826337	RNA PCR Primer, Index 27 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6267	0.8407645362915939	No Hit
GCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	3653	0.4900770466049453	Illumina PCR Primer Index 6 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT	3130	0.41991271718408946	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTC	2704	0.3627616572734115	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCT	2418	0.3243926358310314	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCCTATTT	1807	0.24242245365867404	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTA	1689	0.22659187837825145	No Hit
TGTGAGGGGATCTGTACGGGACGACATCATCCAAAACGATCTTTTTCTGTA	1318	0.17681947643726195	No Hit
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	1203	0.1613913734097315	RNA PCR Primer, Index 27 (96% over 25bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	798	0.10705761926929822	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.68314835261399E-4	0.0	0.0	0.1927842091353152	0.0
2	2.68314835261399E-4	0.0	0.0	1.009534567671014	0.0
3	2.68314835261399E-4	0.0	0.0	1.1741457191038822	0.0
4	2.68314835261399E-4	0.0	0.0	1.858616863855711	0.0
5	2.68314835261399E-4	0.0	0.0	4.002184082759028	0.0
6	2.68314835261399E-4	0.0	0.0	4.393923742240671	0.0
7	2.68314835261399E-4	0.0	0.0	5.032244735327539	0.0
8	2.68314835261399E-4	0.0	0.0	6.131933087646383	0.0
9	2.68314835261399E-4	0.0	0.0	6.437677842426747	0.0
10	2.68314835261399E-4	0.0	0.0	8.759808584196524	0.0
11	2.68314835261399E-4	0.0	0.0	9.337892896767208	0.0
12	2.68314835261399E-4	0.0	0.0	11.991124145249552	0.0
13	2.68314835261399E-4	0.0	0.0	12.522119204231862	0.0
14	2.68314835261399E-4	0.0	0.0	12.704975764462505	0.0
15	2.68314835261399E-4	0.0	0.0	13.115631619830076	0.0
16	2.68314835261399E-4	0.0	0.0	13.523201854592141	0.0
17	2.68314835261399E-4	0.0	0.0	13.839008415694808	0.0
18	2.68314835261399E-4	0.0	0.0	14.4166902560126	0.0
19	2.68314835261399E-4	0.0	0.0	14.88664368997294	0.0
20	2.68314835261399E-4	0.0	0.0	15.164215387050858	0.0
21	2.68314835261399E-4	0.0	0.0	15.375781634654471	0.0
22	2.68314835261399E-4	0.0	0.0	15.606532392979274	0.0
23	2.68314835261399E-4	0.0	0.0	15.808573463931108	0.0
24	2.68314835261399E-4	0.0	0.0	15.979490013992619	0.0
25	2.68314835261399E-4	0.0	0.0	16.11552563547015	0.0
26	2.68314835261399E-4	0.0	0.0	16.245926645407188	0.0
27	2.68314835261399E-4	0.0	0.0	16.415501621292393	0.0
28	2.68314835261399E-4	0.0	0.0	16.545097686723647	0.0
29	2.68314835261399E-4	0.0	0.0	16.690390170017697	0.0
30	2.68314835261399E-4	0.0	0.0	16.858355256891333	0.0
31	5.36629670522798E-4	0.0	0.0	17.004855156944053	0.0
32	5.36629670522798E-4	0.0	0.0	17.14598876029155	0.0
33	6.707870881534976E-4	0.0	0.0	17.29154955842086	0.0
34	6.707870881534976E-4	0.0	0.0	17.44542811644327	0.0
35	8.049445057841971E-4	0.0	0.0	17.603599711829865	0.0
36	8.049445057841971E-4	0.0	0.0	17.75425849182914	0.0
37	8.049445057841971E-4	0.0	0.0	17.91283255946863	0.0
38	8.049445057841971E-4	0.0	0.0	18.060137404027138	0.0
39	8.049445057841971E-4	0.0	0.0	18.19872201643965	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAT	35	1.2111559E-7	45.000004	30
CGGGTAG	35	1.2111559E-7	45.000004	6
GAATCCC	35	1.2111559E-7	45.000004	12
GGATCGA	65	0.0	45.000004	9
GTTAGCG	35	1.2111559E-7	45.000004	1
CGTTCTA	35	1.2111559E-7	45.000004	29
TCTAGCA	35	1.2111559E-7	45.000004	35
AGTCTAC	35	1.2111559E-7	45.000004	38
GTCCGGA	35	1.2111559E-7	45.000004	27
CGTACCT	35	1.2111559E-7	45.000004	36
ACCGTAG	35	1.2111559E-7	45.000004	1
GTCCAAT	35	1.2111559E-7	45.000004	41
TATGTAG	130	0.0	45.000004	1
TACGTAG	35	1.2111559E-7	45.000004	1
TGATCGA	20	7.03169E-4	45.0	34
TCGCCCG	20	7.03169E-4	45.0	37
AGCGTCG	25	3.889481E-5	45.0	25
TCGTTGC	20	7.03169E-4	45.0	44
TCGTTCC	25	3.889481E-5	45.0	13
GTCGCCC	25	3.889481E-5	45.0	12
>>END_MODULE