SRR3548297_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548297_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	505070
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCG	6357	1.2586374165957193	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGC	6325	1.2523016611558795	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6264	1.2402241273486845	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTC	5597	1.1081632248995188	RNA PCR Primer, Index 27 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	3037	0.6013027897123171	Illumina PCR Primer Index 6 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	2184	0.4324153087690815	RNA PCR Primer, Index 27 (96% over 25bp)
GAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT	1800	0.3563862434910013	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTC	1716	0.3397548854614212	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCCTATTT	1689	0.33440909180905615	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCT	1606	0.3179757261369711	No Hit
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	1453	0.287682895440236	RNA PCR Primer, Index 27 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTA	1226	0.24273863028887085	No Hit
TCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	968	0.19165660205516066	Illumina PCR Primer Index 6 (95% over 22bp)
TGTGAGGGGATCTGTACGGGACGACATCATCCAAAACGATCTTTTTCTGTA	890	0.17621319817055062	No Hit
ACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	765	0.15146415348367553	Illumina PCR Primer Index 6 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	628	0.12433920050686044	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGC	533	0.10552992654483537	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGG	55	1.8189894E-12	45.0	3
CGGGTCA	25	3.8876893E-5	45.0	6
AAGTACG	25	3.8876893E-5	45.0	1
GGTACGC	25	3.8876893E-5	45.0	9
CGAACTT	20	7.029527E-4	45.0	14
TCACCCG	20	7.029527E-4	45.0	37
GTACAAC	20	7.029527E-4	45.0	44
CACGGGC	20	7.029527E-4	45.0	4
CAACGCC	20	7.029527E-4	45.0	39
TCCGATA	25	3.8876893E-5	45.0	12
TAGACCC	25	3.8876893E-5	45.0	42
GAGTCAC	20	7.029527E-4	45.0	9
CGGCCCA	25	3.8876893E-5	45.0	33
TTTAACG	20	7.029527E-4	45.0	1
CGTGATT	20	7.029527E-4	45.0	22
ATAATCG	20	7.029527E-4	45.0	43
CATTGCG	20	7.029527E-4	45.0	1
TGGGTAC	70	0.0	45.0	6
GGCCGAA	20	7.029527E-4	45.0	21
ATTCGTG	20	7.029527E-4	45.0	34

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content