FastQCFastQC Report
Sat 18 Jun 2016
SRR3548296_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548296_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533306
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGC241654.531169722448276No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCG198423.720565678991048No Hit
GAATCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTC185613.4803658687507735No Hit
GCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC56101.051928911356707Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCT55471.0401158059350541No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTC40590.7611015064522056No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCT39470.7401004301470451No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34390.6448455483343521No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAACCAGAT29420.5516532722302018No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAACCAGATCGTA27180.5096511196198805No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAACCAGATCGT10940.20513551319505124No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGAACCAGATCGTAT9430.1768215621050579No Hit
CTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGCT9110.1708212545892977Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCGAACCAGAT7090.13294431339606155No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTT6760.12675649627043387No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATG6600.12375634251255377No Hit
CCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC6290.1179435446066611Illumina Single End Adapter 1 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5340.10013013166924804No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGAAT302.163304E-645.00000426
CTGTATT302.163304E-645.00000422
TTCACGG302.163304E-645.0000042
GTGATTA302.163304E-645.00000435
CTAAGCG302.163304E-645.0000041
CCTAACA302.163304E-645.00000427
CTTGGTC302.163304E-645.00000429
GTAATCC302.163304E-645.0000043
TAGCATA302.163304E-645.00000430
TAGCAAC302.163304E-645.00000412
CTAACAC302.163304E-645.00000428
CGTAGTT302.163304E-645.00000429
CGGATAG302.163304E-645.00000420
TTGACGA600.045.00000440
CCTCAAT302.163304E-645.00000416
TCGTCTA302.163304E-645.00000432
GGACGAA302.163304E-645.0000048
CGAGACC302.163304E-645.00000439
CGCACTG302.163304E-645.0000049
CACCGGT600.045.00000416
Produced by FastQC (version 0.11.5)