FastQCFastQC Report
Sat 18 Jun 2016
SRR3548294_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548294_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867656
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGC192352.216892408973141No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCG166411.9179259983219157No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTC141611.6320984353245989No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59510.6858708981439648No Hit
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC46060.5308555464377587TruSeq Adapter, Index 14 (95% over 21bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC38810.4472970854808818No Hit
GAACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT36310.4184838230819587No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTC31220.3598200208377514No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCT27050.31175949915634765No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCATGTTT22460.2588583493919249No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC18850.21725199848788No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTA16650.19189632757682767No Hit
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT8900.10257521414016614Illumina Paired End PCR Primer 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGT453.8380676E-1045.00000416
GATCGCC302.164883E-645.00000425
CTATACG302.164883E-645.0000041
TAGCGAT453.8380676E-1045.00000416
CACGCAG302.164883E-645.0000041
CAATTAG302.164883E-645.00000417
AGTGCGC302.164883E-645.00000422
TATTCGT302.164883E-645.00000444
TAAGGCG302.164883E-645.0000041
TTGTTCG302.164883E-645.00000417
AACTACG302.164883E-645.0000041
TTCGCAA453.8380676E-1045.00000415
CGTCCAG302.164883E-645.00000417
TCAAGTA302.164883E-645.00000423
TATCGAA302.164883E-645.00000439
CGATAAG302.164883E-645.00000410
GTAGTAC453.8380676E-1045.00000440
TGCGTAG453.8380676E-1045.0000041
GTTGCAA453.8380676E-1045.00000424
TATCCCG302.164883E-645.00000410
Produced by FastQC (version 0.11.5)