FastQCFastQC Report
Sat 18 Jun 2016
SRR3548293_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548293_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538967
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGC82581.5321902825219356No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCG78751.461128417880872No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTC69251.284865307152386No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64221.1915386285245664No Hit
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC35330.6555132317934121TruSeq Adapter, Index 14 (95% over 21bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC19890.3690392918304831No Hit
GAACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT19220.3566081040212109No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTC18390.34120827434703793No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCATGTTT18100.33582761096690517No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCT15980.29649310625696934No Hit
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT15870.29445216497485005Illumina Paired End PCR Primer 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC13180.244541873621205TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTA10750.1994556252980238No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC8740.16216206187020726No Hit
TCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC8490.15752355895629974TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGC7010.13006362170596716No Hit
ACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC6190.11484933214835046No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGCG302.1633477E-645.0000041
TCGCACA302.1633477E-645.00000419
CCTAGAA302.1633477E-645.00000411
CTATACT302.1633477E-645.00000415
GCGCCCA302.1633477E-645.00000433
CGCGAGG850.045.0000042
ATGTCGT302.1633477E-645.0000041
TCAGATC600.045.00000417
ACTACTT302.1633477E-645.00000430
GGCGCGA600.045.0000048
CACGTGC302.1633477E-645.00000439
TAATCAC253.888039E-545.033
AACGTAG351.2103192E-745.01
CGGCGAA253.888039E-545.031
TCGTTAC253.888039E-545.043
GTCGCAT207.029949E-445.044
CGAACAC351.2103192E-745.025
TCCGCAA207.029949E-445.045
ATTAGCG453.8380676E-1045.01
GATCGCC253.888039E-545.025
Produced by FastQC (version 0.11.5)