##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548293_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	538967
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.41889021034683	30.0	25.0	31.0	16.0	33.0
2	27.822285965560045	31.0	25.0	33.0	16.0	33.0
3	27.821612454936943	31.0	25.0	33.0	16.0	34.0
4	32.03257156746146	35.0	32.0	35.0	19.0	37.0
5	33.089278193284564	35.0	32.0	35.0	28.0	37.0
6	31.7996649145495	35.0	32.0	35.0	25.0	37.0
7	33.694573137130845	35.0	33.0	35.0	30.0	37.0
8	33.52541064666297	35.0	33.0	37.0	28.0	37.0
9	36.21056576747741	39.0	35.0	39.0	31.0	39.0
10	35.30038759330348	37.0	34.0	39.0	30.0	39.0
11	35.47950616642577	37.0	35.0	39.0	30.0	39.0
12	35.35324240630688	37.0	34.0	39.0	30.0	39.0
13	35.100078483469304	37.0	34.0	39.0	27.0	39.0
14	35.7496228897131	38.0	34.0	40.0	27.0	41.0
15	36.3853204370583	38.0	34.0	40.0	31.0	41.0
16	36.171409381279375	37.0	34.0	40.0	31.0	41.0
17	35.76221364202261	37.0	34.0	40.0	30.0	41.0
18	35.76663877380248	37.0	34.0	39.0	30.0	40.0
19	35.26967328240875	37.0	34.0	39.0	29.0	40.0
20	35.410557603712284	37.0	34.0	39.0	30.0	40.0
21	35.67366276599495	37.0	34.0	39.0	30.0	40.0
22	36.06281275105897	37.0	34.0	40.0	31.0	41.0
23	36.28246812884648	38.0	35.0	40.0	31.0	41.0
24	36.23325732373225	38.0	35.0	40.0	31.0	41.0
25	35.574888629545036	37.0	34.0	40.0	30.0	41.0
26	35.71023828917169	37.0	34.0	40.0	30.0	41.0
27	35.88937541630564	37.0	34.0	40.0	30.0	41.0
28	35.74320505708142	37.0	35.0	40.0	30.0	41.0
29	35.84239665879358	38.0	35.0	40.0	30.0	41.0
30	35.38273215243234	37.0	34.0	40.0	29.0	41.0
31	35.512016505648766	37.0	34.0	40.0	30.0	41.0
32	35.04759289529785	37.0	34.0	40.0	27.0	41.0
33	35.04614382698755	38.0	34.0	40.0	26.0	41.0
34	34.92934261281303	38.0	34.0	40.0	25.0	41.0
35	34.751376243814555	38.0	34.0	40.0	24.0	41.0
36	34.53578233917847	38.0	34.0	40.0	23.0	41.0
37	34.578796104399714	38.0	34.0	40.0	23.0	41.0
38	34.53105477700861	37.0	34.0	40.0	23.0	41.0
39	34.293359333688336	37.0	34.0	40.0	22.0	41.0
40	34.231858351253415	37.0	34.0	40.0	22.0	41.0
41	34.011481222412506	37.0	33.0	40.0	21.0	41.0
42	34.13829232587524	37.0	34.0	40.0	22.0	41.0
43	34.13825892865426	37.0	34.0	40.0	22.0	41.0
44	34.20419246447371	37.0	34.0	40.0	22.0	41.0
45	34.23789582664616	37.0	34.0	40.0	23.0	41.0
46	33.955950920928366	37.0	34.0	40.0	21.0	41.0
47	33.90796468058341	37.0	33.0	40.0	22.0	41.0
48	33.982778166381244	37.0	34.0	40.0	22.0	41.0
49	34.06897082752747	37.0	34.0	40.0	22.0	41.0
50	33.90469360832852	36.0	34.0	40.0	21.0	41.0
51	31.93231125467793	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	22.0
11	12.0
12	14.0
13	24.0
14	41.0
15	44.0
16	119.0
17	230.0
18	527.0
19	1145.0
20	1952.0
21	2916.0
22	3920.0
23	4799.0
24	6298.0
25	8449.0
26	10378.0
27	11200.0
28	11227.0
29	12108.0
30	14920.0
31	20618.0
32	29795.0
33	45329.0
34	58532.0
35	54952.0
36	71012.0
37	87222.0
38	67825.0
39	13328.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.98111387153574	22.00988186660779	24.088858872621145	16.920145389235334
2	31.692849469448035	29.745234865956544	25.458701553156317	13.103214111439105
3	25.942404637018594	28.309896524276994	32.43686533683881	13.310833501865607
4	23.86138668972312	24.148417250035717	37.77912191284439	14.21107414739678
5	26.339460486449077	27.78407583395644	30.326532051127437	15.549931628467048
6	18.46179821770164	39.540268699196794	29.199375843047903	12.79855724005366
7	70.33974250742624	5.383075401647967	20.74023084901302	3.5369512419127704
8	67.46850920371749	10.069447665627024	15.160297383698818	7.301745746956678
9	63.226876599123884	5.830227082548653	17.760642117235378	13.18225420109209
10	41.9667623435201	21.26883464108192	23.14891264214692	13.615490373251053
11	35.387138730200554	19.996771601971922	28.440145686099523	16.175943981728008
12	30.555303014841357	17.47954884065258	31.722721428213603	20.242426716292464
13	22.5622347935959	26.867878738401423	35.515161410624394	15.054725057378281
14	15.57497954420215	34.73329535945614	29.296227783890295	20.39549731245141
15	12.864980601780815	21.992997716001163	48.207775244124406	16.934246438093613
16	13.847972139296097	22.31027131531244	39.947343714921324	23.89441283047014
17	14.54115001475044	23.40532908322773	33.43952412670906	28.61399677531277
18	17.19864110418634	22.652592830358817	36.14636888714893	24.002397178305905
19	20.896084546920314	25.52122857243579	31.216011369898343	22.366675510745555
20	23.92651127063438	22.670033601315108	34.759456515890584	18.64399861215993
21	19.666510194501704	29.681965686210845	32.40049947399377	18.251024645293683
22	17.10939630812276	22.545350642989273	33.49908250412363	26.846170544764338
23	17.830405201060547	29.642445641384352	30.027626923355232	22.49952223419987
24	20.323136666994454	23.03369222976546	34.36629700890778	22.276874094332307
25	16.36760692213067	28.241061141034606	32.268765991238794	23.122565945595927
26	16.154606868324034	26.891998953553742	32.698662441299746	24.254731736822478
27	19.858544215137474	27.44750606252331	30.294433610963196	22.399516111376023
28	14.024606330257697	26.601999751376244	37.63180306029868	21.74159085806738
29	16.57875157477174	22.59730187562504	34.46648867184818	26.357457877755035
30	17.237418988546608	25.862251306666273	32.49197075145603	24.408358953331096
31	23.19975063408335	24.035423319052928	25.240135295852994	27.524690751010727
32	22.61381494599855	28.013032337786914	27.12986138297892	22.243291333235614
33	21.40928850931504	23.38844493262111	28.948525605463786	26.25374095260007
34	20.12702076379444	23.346883946512495	29.231103202979032	27.294992086714025
35	16.474106949033985	23.31589874704759	32.084524655498385	28.125469648420033
36	20.959168186549455	21.454189217521666	32.10363528750369	25.483007308425194
37	19.375026671391755	26.574354274009355	31.960954937871893	22.089664116726997
38	19.047548365669883	25.96763809287025	29.350962118274403	25.633851423185465
39	21.357337276679278	25.734228626242423	31.5932144268573	21.315219670220998
40	21.702627433590553	21.3020463219455	35.517573432139635	21.477752812324315
41	19.011924663291076	21.430254542485905	31.775043741082477	27.782777053140546
42	20.984772722634222	21.35659511621305	32.893665103800416	24.764967057352305
43	22.160911521484618	21.216326788096488	31.68468570431956	24.938075986099335
44	20.351709844944125	24.430994847550963	31.00616549807317	24.211129809431746
45	18.78686450190828	25.524939374766912	28.16072227056573	27.527473852759076
46	24.536752713988054	25.920510903264947	27.797991342698165	21.744745040048834
47	18.10426241309765	22.598786196557487	35.32869359348532	23.96825779685955
48	21.011490499418333	21.28163690912431	31.20914638558576	26.4977262058716
49	19.144029226279162	17.738191763132065	37.73885970755167	25.378919303037108
50	19.81568444821297	21.54027983160379	33.6410206932892	25.003015026894037
51	18.275887020912226	22.58320082676676	28.59971018633794	30.541201965983074
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	588.0
1	565.0
2	542.0
3	5416.5
4	10291.0
5	7073.5
6	3856.0
7	3976.5
8	4097.0
9	4244.5
10	4392.0
11	4314.5
12	4237.0
13	4062.0
14	3887.0
15	3673.0
16	3459.0
17	3322.0
18	3185.0
19	3108.5
20	3032.0
21	3048.0
22	3064.0
23	3018.0
24	2972.0
25	2904.0
26	3435.5
27	4035.0
28	4238.5
29	4442.0
30	5307.5
31	6173.0
32	6903.0
33	7633.0
34	8185.0
35	8737.0
36	9556.5
37	10376.0
38	11568.5
39	12761.0
40	16291.5
41	19822.0
42	26847.0
43	33872.0
44	39030.5
45	44189.0
46	62906.5
47	81624.0
48	72869.0
49	64114.0
50	59726.5
51	55339.0
52	44974.5
53	34610.0
54	29929.0
55	25248.0
56	22799.5
57	20351.0
58	18322.0
59	16293.0
60	15175.5
61	14058.0
62	11878.0
63	9698.0
64	7829.0
65	5960.0
66	4632.5
67	3305.0
68	2881.0
69	2457.0
70	2066.0
71	1675.0
72	1242.0
73	809.0
74	623.0
75	332.5
76	228.0
77	219.5
78	211.0
79	129.5
80	48.0
81	29.5
82	11.0
83	8.0
84	5.0
85	5.5
86	6.0
87	3.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	538967.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.924295607322964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.8488724645787	22.51723799862259
2	9.63548851121067	5.573994360384434
3	3.6614488841779718	3.1771448963119946
4	1.880852430252649	2.1760932674551765
5	1.0817318504967541	1.5644165905812306
6	0.6931885398188476	1.2029994142397349
7	0.5096730813670686	1.0319354407989383
8	0.3760265463533941	0.8701042386341041
9	0.25916839901353034	0.6746637046629583
>10	2.6067560453808025	18.13454784892453
>50	1.1799719322609827	24.3548936036581
>100	0.2545164486161176	9.308347161932502
>500	0.003885747307116299	0.7685774049225037
>1k	0.0058286209606744485	3.122825717149174
>5k	0.0025904982047441996	5.522218351722011
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGC	8258	1.5321902825219356	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCG	7875	1.461128417880872	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTC	6925	1.284865307152386	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6422	1.1915386285245664	No Hit
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	3533	0.6555132317934121	TruSeq Adapter, Index 14 (95% over 21bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1989	0.3690392918304831	No Hit
GAACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT	1922	0.3566081040212109	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTC	1839	0.34120827434703793	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCATGTTT	1810	0.33582761096690517	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCT	1598	0.29649310625696934	No Hit
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT	1587	0.29445216497485005	Illumina Paired End PCR Primer 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	1318	0.244541873621205	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTA	1075	0.1994556252980238	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	874	0.16216206187020726	No Hit
TCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	849	0.15752355895629974	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGC	701	0.13006362170596716	No Hit
ACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	619	0.11484933214835046	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.32525182432319605	0.0
2	0.0	0.0	0.0	1.631083164646444	0.0
3	0.0	0.0	0.0	2.070998781001434	0.0
4	0.0	0.0	0.0	3.1369267506173846	0.0
5	0.0	0.0	0.0	6.003336011295683	0.0
6	0.0	0.0	0.0	7.288015778331512	0.0
7	0.0	0.0	0.0	8.419810489324949	0.0
8	0.0	0.0	0.0	10.738690866045602	0.0
9	0.0	0.0	0.0	11.473429727608554	0.0
10	0.0	0.0	0.0	14.437247549478911	0.0
11	0.0	0.0	0.0	15.890768822580974	0.0
12	0.0	0.0	0.0	19.018975187720212	0.0
13	1.8554011655630122E-4	0.0	0.0	19.738128679492437	0.0
14	1.8554011655630122E-4	0.0	0.0	20.01532561362755	0.0
15	1.8554011655630122E-4	0.0	0.0	20.593839697050097	0.0
16	1.8554011655630122E-4	0.0	0.0	21.387951395911067	0.0
17	1.8554011655630122E-4	0.0	0.0	22.134379284817065	0.0
18	7.421604662252049E-4	0.0	0.0	23.259494551614477	0.0
19	7.421604662252049E-4	0.0	0.0	24.123183794184058	0.0
20	7.421604662252049E-4	0.0	0.0	24.664033233945677	0.0
21	7.421604662252049E-4	0.0	0.0	25.210448877203984	0.0
22	7.421604662252049E-4	0.0	0.0	25.75092723673249	0.0
23	7.421604662252049E-4	0.0	0.0	26.263945659010663	0.0
24	7.421604662252049E-4	0.0	0.0	26.652281122963	0.0
25	7.421604662252049E-4	0.0	0.0	27.00109654208885	0.0
26	7.421604662252049E-4	0.0	0.0	27.340449415270324	0.0
27	7.421604662252049E-4	0.0	0.0	27.7107874879167	0.0
28	7.421604662252049E-4	0.0	0.0	28.043089836669036	0.0
29	7.421604662252049E-4	0.0	0.0	28.34589130688892	0.0
30	0.0011132406993378074	0.0	0.0	28.715672759185626	0.0
31	0.001669861049006711	0.0	0.0	29.057252113765777	0.0
32	0.001669861049006711	0.0	0.0	29.363764386316788	0.0
33	0.001669861049006711	0.0	0.0	29.68122352574462	0.0
34	0.001669861049006711	0.0	0.0	29.970295027339336	0.0
35	0.001669861049006711	0.0	0.0	30.339519859286376	0.0
36	0.001669861049006711	0.0	0.0	30.656051298131427	0.0
37	0.001669861049006711	0.0	0.0	30.968500854412238	0.0
38	0.001669861049006711	0.0	0.0	31.247553189712914	0.0
39	0.001669861049006711	0.0	0.0	31.55072574016591	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGCG	30	2.1633477E-6	45.000004	1
TCGCACA	30	2.1633477E-6	45.000004	19
CCTAGAA	30	2.1633477E-6	45.000004	11
CTATACT	30	2.1633477E-6	45.000004	15
GCGCCCA	30	2.1633477E-6	45.000004	33
CGCGAGG	85	0.0	45.000004	2
ATGTCGT	30	2.1633477E-6	45.000004	1
TCAGATC	60	0.0	45.000004	17
ACTACTT	30	2.1633477E-6	45.000004	30
GGCGCGA	60	0.0	45.000004	8
CACGTGC	30	2.1633477E-6	45.000004	39
TAATCAC	25	3.888039E-5	45.0	33
AACGTAG	35	1.2103192E-7	45.0	1
CGGCGAA	25	3.888039E-5	45.0	31
TCGTTAC	25	3.888039E-5	45.0	43
GTCGCAT	20	7.029949E-4	45.0	44
CGAACAC	35	1.2103192E-7	45.0	25
TCCGCAA	20	7.029949E-4	45.0	45
ATTAGCG	45	3.8380676E-10	45.0	1
GATCGCC	25	3.888039E-5	45.0	25
>>END_MODULE