SRR3548289_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548289_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	497269
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7834	1.5754048613527087	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGC	7144	1.4366469657268	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCG	6848	1.3771218394872795	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTC	6655	1.3383098483919167	No Hit
GCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTGC	4750	0.955217397424734	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTGCT	2909	0.5849952440228529	TruSeq Adapter, Index 13 (95% over 22bp)
AATGCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTC	2174	0.4371879204213414	No Hit
GAACTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCT	2058	0.4138605060842321	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTC	1823	0.36660238221164	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAAAGCTGT	1773	0.35654746223874806	No Hit
TCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTGC	1646	0.33100796550760253	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTGC	1583	0.31833876634175867	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCT	1563	0.3143167983526019	No Hit
ACTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTGC	1289	0.25921583690115413	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTA	1239	0.2491609169282622	No Hit
AATGTGAATGATACCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTA	1225	0.24634553933585243	No Hit
AATGTGAATGATACGGCTGTCTCTTATACACATCTGACGCCAAAGCTGTCG	993	0.19969071066163385	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	921	0.18521162590066945	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTT	901	0.18118965791151267	No Hit
TATGATGGGAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAAAA	767	0.15424247238416228	No Hit
AATGTGAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAAA	646	0.1299095660497638	No Hit
AATGTGAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAAAGCTG	632	0.12709418845735407	No Hit
AATGTGAATGATCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATG	616	0.12387661406602865	No Hit
AGCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTG	544	0.10939752930506426	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCC	542	0.10899533250614858	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGC	519	0.1043700693186183	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.029422E-4	45.0	25
GTCATGG	20	7.029422E-4	45.0	2
ATCCTGC	20	7.029422E-4	45.0	20
AAGTATG	45	3.8380676E-10	45.0	1
TAGAGTT	20	7.029422E-4	45.0	13
GTGCAAT	20	7.029422E-4	45.0	14
CGGGTAC	20	7.029422E-4	45.0	6
GGTACTC	25	3.8876024E-5	45.0	8
AGCCGGA	20	7.029422E-4	45.0	45
TCGTTGG	20	7.029422E-4	45.0	2
GCCCATT	20	7.029422E-4	45.0	39
TCGTTCA	25	3.8876024E-5	45.0	16
CTCACGA	20	7.029422E-4	45.0	24
TCGCAGA	20	7.029422E-4	45.0	12
CGAACCC	20	7.029422E-4	45.0	34
ATTAGTG	25	3.8876024E-5	45.0	1
GTTCTAG	20	7.029422E-4	45.0	1
ATGATCA	20	7.029422E-4	45.0	15
GTCGAAT	25	3.8876024E-5	45.0	43
TTGTGCG	20	7.029422E-4	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content