##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548289_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	497269
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.44168045866523	30.0	25.0	31.0	16.0	33.0
2	27.41163635778623	30.0	25.0	31.0	16.0	33.0
3	27.58039411264326	30.0	25.0	33.0	16.0	34.0
4	31.77655353541041	33.0	30.0	35.0	19.0	37.0
5	33.042938530252236	35.0	32.0	35.0	28.0	37.0
6	31.780314075480273	35.0	32.0	35.0	25.0	37.0
7	33.588777904916654	35.0	33.0	35.0	30.0	37.0
8	33.173986313242935	35.0	33.0	36.0	28.0	37.0
9	35.82398661489053	37.0	35.0	39.0	30.0	39.0
10	35.1515196000555	37.0	34.0	39.0	30.0	39.0
11	35.60579485147878	37.0	35.0	39.0	31.0	39.0
12	35.59248817038665	37.0	34.0	39.0	31.0	39.0
13	35.45426921847129	37.0	34.0	39.0	30.0	39.0
14	35.98449129143381	38.0	34.0	40.0	29.0	41.0
15	36.53378151463293	38.0	35.0	40.0	31.0	41.0
16	36.247686865660235	38.0	34.0	40.0	32.0	41.0
17	35.86254321101858	37.0	34.0	39.0	30.0	41.0
18	35.91150866030257	37.0	34.0	39.0	30.0	40.0
19	35.33254837924745	37.0	34.0	39.0	28.0	40.0
20	35.457858824901614	37.0	34.0	39.0	30.0	40.0
21	35.64747450575041	37.0	34.0	39.0	30.0	40.0
22	36.03548381258434	38.0	34.0	40.0	31.0	40.0
23	36.26801992482942	38.0	35.0	40.0	31.0	41.0
24	36.261588798014756	38.0	35.0	40.0	31.0	41.0
25	35.30755788114682	37.0	34.0	40.0	27.0	41.0
26	35.46216031966602	37.0	34.0	40.0	29.0	41.0
27	35.64724525357503	37.0	34.0	40.0	30.0	41.0
28	35.37701726831956	37.0	34.0	40.0	28.0	41.0
29	35.435088453131	37.0	34.0	40.0	29.0	41.0
30	34.70479760451587	37.0	34.0	40.0	25.0	41.0
31	34.76227152708092	37.0	34.0	40.0	25.0	40.0
32	34.504911828406755	37.0	33.0	40.0	25.0	40.0
33	34.35184377067543	37.0	33.0	40.0	23.0	41.0
34	34.28676430664289	37.0	34.0	40.0	23.0	41.0
35	34.15672201564948	37.0	34.0	40.0	21.0	41.0
36	33.76698527356421	37.0	33.0	40.0	18.0	41.0
37	33.65950019003799	37.0	33.0	40.0	18.0	41.0
38	33.63821392445538	37.0	33.0	40.0	18.0	41.0
39	33.66431448572101	37.0	33.0	40.0	18.0	41.0
40	33.66979039513824	37.0	33.0	40.0	18.0	41.0
41	33.50651056068245	37.0	33.0	40.0	18.0	40.0
42	33.61307059157116	37.0	33.0	40.0	18.0	41.0
43	33.55741459853721	37.0	33.0	40.0	18.0	41.0
44	33.57004961097515	37.0	33.0	40.0	18.0	41.0
45	33.643203175745924	37.0	33.0	40.0	18.0	41.0
46	33.37225123625241	37.0	33.0	40.0	17.0	40.0
47	33.35385475467001	37.0	33.0	40.0	18.0	40.0
48	33.51468118865242	37.0	33.0	40.0	18.0	40.0
49	33.5306383466494	37.0	33.0	40.0	18.0	40.0
50	33.34096836923275	37.0	33.0	40.0	15.0	40.0
51	31.34168830150281	35.0	29.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	13.0
11	14.0
12	3.0
13	13.0
14	21.0
15	40.0
16	117.0
17	293.0
18	661.0
19	1489.0
20	2576.0
21	3833.0
22	4853.0
23	5894.0
24	7075.0
25	8510.0
26	9644.0
27	9951.0
28	9810.0
29	10749.0
30	13765.0
31	18780.0
32	27220.0
33	40566.0
34	54645.0
35	61445.0
36	77157.0
37	78243.0
38	43718.0
39	6164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.58067967237049	25.66116126281751	24.682013155857295	15.076145908954711
2	29.063142886445764	32.51459471634065	25.308836866967376	13.113425530246205
3	22.87092097034	28.32430736683767	37.2631312227386	11.541640440083738
4	25.29194460141292	24.402285282211437	38.18235200666038	12.123418109715264
5	23.558074201287432	26.245352113242532	34.40331088404867	15.793262801421362
6	19.331388041482576	37.80428701567964	30.4724404698463	12.39188447299148
7	65.65540984859301	8.227538817018555	21.74396553977827	4.3730857946101604
8	60.95694684366008	11.974605294116463	16.91358198480098	10.15486587742248
9	56.190311481310914	6.231436104000048	23.45953598555309	14.118716429135942
10	37.412949530334686	26.138769961529878	23.055529301042295	13.392751207093143
11	26.085679984073007	24.152923266883718	32.55320560903655	17.20819114000672
12	23.267688112470314	15.579696301197139	40.387798153514495	20.764817432818052
13	20.293442784488878	27.77731972031234	39.3732567282497	12.555980766949077
14	13.060134454389877	35.016459903995624	30.20075653217876	21.72264910943574
15	10.595070273835692	23.50619081422731	49.060166630133786	16.83857228180321
16	11.303741033525114	25.939682546066617	39.20473626950403	23.551840150904237
17	10.466166199783215	25.891217831797274	32.63103068962674	31.011585278792765
18	14.512869292073304	24.789399701167778	38.276868254405564	22.420862752353354
19	14.857149751945123	31.00736221240415	31.587732193239475	22.54775584241125
20	17.358411644401723	27.33972960309209	34.822601046918265	20.479257705587923
21	14.205792036101183	33.51827682803473	33.12734153948868	19.148589596375405
22	12.934850151527645	26.634879712992365	31.47350830234742	28.95676183313257
23	11.596741401535185	31.737550500835564	30.90419873348228	25.761509364146974
24	15.924580056267331	26.31915522584356	32.728362314964336	25.027902402924774
25	12.71384301052348	31.809744826240927	30.385364862881058	25.091047300354536
26	11.444308814746144	30.76664742825312	32.575929728175296	25.213114028825444
27	17.3193985549069	31.559779515714837	29.2781170754662	21.842704853912068
28	10.970722084022935	32.12828469098214	35.04903784470779	21.851955380287126
29	13.548200269874053	27.74916594438825	29.580770166650243	29.121863619087456
30	17.459564139329014	34.09020067609282	27.742328598806683	20.707906585771486
31	18.884949594686177	34.24564973887373	21.91107830972773	24.95832235671236
32	20.056749968327004	34.49078868781284	22.847191359203972	22.605269984656193
33	21.575847277831517	31.224749582218074	22.10574156040292	25.093661579547486
34	17.463988304117088	28.30158324769893	25.039566110093332	29.194862338090648
35	15.081374467340613	30.839646147256317	26.63608630338911	27.44289308201396
36	26.06195037293698	26.30427394428368	25.380427897174368	22.253347785604973
37	17.91485091570156	32.38548954388872	28.844347827835637	20.85531171257408
38	20.462968735231836	32.725546937371924	24.338939286382217	22.47254504101402
39	25.540703321542264	26.681534541666586	25.405565197106593	22.372196939684557
40	23.886266789202626	22.98112289324289	31.074730176222527	22.057880141331957
41	19.89868662635314	23.496135894254415	25.232620573572856	31.372556905819586
42	23.265677128475733	22.87976929991614	31.319466928362715	22.535086643245407
43	26.416889047980067	23.351143948245316	27.267535277686726	22.96443172608789
44	20.196714454349657	27.834431665758373	25.555182406303228	26.41367147358874
45	18.01801439462343	25.72913252183426	27.00188429200292	29.25096879153939
46	28.038948738006997	25.357502679636173	25.56925929426528	21.034289288091557
47	17.17159123130539	22.78404646177421	34.68786511928151	25.356497187638883
48	19.83896040171416	21.83224773714026	29.75069831419212	28.578093546953458
49	22.302415795072687	17.89836084694602	34.279233171583186	25.519990186398108
50	20.048907130748148	19.66682017177825	33.31335755898719	26.97091513848641
51	18.105090001588678	20.592275006083227	25.707212796293355	35.59542219603474
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1014.0
1	860.5
2	707.0
3	5107.5
4	9508.0
5	6071.0
6	2634.0
7	2870.5
8	3107.0
9	3643.0
10	4179.0
11	4744.5
12	5310.0
13	5307.0
14	5304.0
15	5375.5
16	5447.0
17	5206.0
18	4965.0
19	4612.5
20	4260.0
21	3949.0
22	3638.0
23	3466.0
24	3294.0
25	3372.0
26	3908.0
27	4366.0
28	4316.0
29	4266.0
30	4349.5
31	4433.0
32	4515.0
33	4597.0
34	4833.5
35	5070.0
36	5886.5
37	6703.0
38	8476.5
39	10250.0
40	14774.5
41	19299.0
42	25103.5
43	30908.0
44	39184.5
45	47461.0
46	69870.5
47	92280.0
48	84591.5
49	76903.0
50	67640.0
51	58377.0
52	45362.5
53	32348.0
54	24730.0
55	17112.0
56	13913.0
57	10714.0
58	8736.5
59	6759.0
60	5112.5
61	3466.0
62	2959.5
63	2453.0
64	1873.0
65	1293.0
66	967.5
67	642.0
68	485.5
69	329.0
70	259.0
71	189.0
72	151.0
73	113.0
74	93.5
75	47.0
76	20.0
77	20.5
78	21.0
79	11.5
80	2.0
81	2.5
82	3.0
83	2.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	497269.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.78936758021037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.43187801376286	26.59016699066595
2	10.141609363104742	7.05640351975908
3	4.101385124535111	4.280537840563766
4	2.2164769375772444	3.08439323657735
5	1.3620245250809606	2.3691985928151498
6	0.944884108369598	1.9723152340061567
7	0.6700357427511077	1.631708382251329
8	0.49729889303868346	1.3840571189723583
9	0.4020792323310145	1.2589273988939222
>10	2.561784192536998	18.338227502076876
>50	0.526271797985598	12.746974824611094
>100	0.129085535354958	7.17736167352285
>500	0.005840974450450589	1.4388861586652502
>1k	0.007009169340540706	4.883393894230093
>5k	0.0023363897801802354	5.787447632388785
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7834	1.5754048613527087	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGC	7144	1.4366469657268	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCG	6848	1.3771218394872795	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTC	6655	1.3383098483919167	No Hit
GCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTGC	4750	0.955217397424734	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTGCT	2909	0.5849952440228529	TruSeq Adapter, Index 13 (95% over 22bp)
AATGCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTC	2174	0.4371879204213414	No Hit
GAACTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCT	2058	0.4138605060842321	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTC	1823	0.36660238221164	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAAAGCTGT	1773	0.35654746223874806	No Hit
TCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTGC	1646	0.33100796550760253	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTGC	1583	0.31833876634175867	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCT	1563	0.3143167983526019	No Hit
ACTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTGC	1289	0.25921583690115413	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTA	1239	0.2491609169282622	No Hit
AATGTGAATGATACCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTA	1225	0.24634553933585243	No Hit
AATGTGAATGATACGGCTGTCTCTTATACACATCTGACGCCAAAGCTGTCG	993	0.19969071066163385	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	921	0.18521162590066945	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTT	901	0.18118965791151267	No Hit
TATGATGGGAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAAAA	767	0.15424247238416228	No Hit
AATGTGAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAAA	646	0.1299095660497638	No Hit
AATGTGAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAAAGCTG	632	0.12709418845735407	No Hit
AATGTGAATGATCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATG	616	0.12387661406602865	No Hit
AGCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTG	544	0.10939752930506426	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCC	542	0.10899533250614858	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGC	519	0.1043700693186183	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6821257709609889	0.0
2	0.0	0.0	0.0	2.8712829474590214	0.0
3	0.0	0.0	0.0	3.8210706880983936	0.0
4	0.0	0.0	0.0	5.604612392889965	0.0
5	0.0	0.0	0.0	10.470992561370204	0.0
6	0.0	0.0	0.0	12.833496558200894	0.0
7	0.0	0.0	0.0	14.723218217906204	0.0
8	0.0	0.0	0.0	19.109174310081666	0.0
9	0.0	0.0	0.0	20.67774182585281	0.0
10	0.0	0.0	0.0	25.205673388045504	0.0
11	0.0	0.0	0.0	28.5483309838337	0.0
12	0.0	0.0	0.0	33.10662840434453	0.0
13	0.0	0.0	0.0	34.5925444779385	0.0
14	0.0	0.0	0.0	35.05446750149316	0.0
15	0.0	0.0	0.0	36.2857930013735	0.0
16	0.0	0.0	0.0	37.56719200271885	0.0
17	0.0	0.0	0.0	39.26285370694735	0.0
18	0.002010983994578387	0.0	0.0	41.63118955736231	0.0
19	0.002010983994578387	0.0	0.0	43.00026746087128	0.0
20	0.002010983994578387	0.0	0.0	44.18473703367795	0.0
21	0.002010983994578387	0.0	0.0	45.051068938542315	0.0
22	0.002010983994578387	0.0	0.0	45.85144056838452	0.0
23	0.002010983994578387	0.0	0.0	46.61722327351997	0.0
24	0.002010983994578387	0.0	0.0	47.4660596176315	0.0
25	0.002010983994578387	0.0	0.0	47.906666210843625	0.0
26	0.002010983994578387	0.0	0.0	48.32153220892515	0.0
27	0.002010983994578387	0.0	0.0	48.73921358459908	0.0
28	0.0022120823940362257	0.0	0.0	49.16473779785186	0.0
29	0.0022120823940362257	0.0	0.0	49.66366292690677	0.0
30	0.0022120823940362257	0.0	0.0	50.16218585916275	0.0
31	0.0022120823940362257	0.0	0.0	50.62913634270385	0.0
32	0.0022120823940362257	0.0	0.0	51.00659803848621	0.0
33	0.0022120823940362257	0.0	0.0	51.40618055820894	0.0
34	0.0022120823940362257	0.0	0.0	51.81521470270618	0.0
35	0.0022120823940362257	0.0	0.0	52.23892903036385	0.0
36	0.0022120823940362257	0.0	0.0	52.5763721446541	0.0
37	0.0022120823940362257	0.0	0.0	52.90436363416983	0.0
38	0.0022120823940362257	0.0	0.0	53.23436610768015	0.0
39	0.0022120823940362257	0.0	0.0	53.55109608682624	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.029422E-4	45.0	25
GTCATGG	20	7.029422E-4	45.0	2
ATCCTGC	20	7.029422E-4	45.0	20
AAGTATG	45	3.8380676E-10	45.0	1
TAGAGTT	20	7.029422E-4	45.0	13
GTGCAAT	20	7.029422E-4	45.0	14
CGGGTAC	20	7.029422E-4	45.0	6
GGTACTC	25	3.8876024E-5	45.0	8
AGCCGGA	20	7.029422E-4	45.0	45
TCGTTGG	20	7.029422E-4	45.0	2
GCCCATT	20	7.029422E-4	45.0	39
TCGTTCA	25	3.8876024E-5	45.0	16
CTCACGA	20	7.029422E-4	45.0	24
TCGCAGA	20	7.029422E-4	45.0	12
CGAACCC	20	7.029422E-4	45.0	34
ATTAGTG	25	3.8876024E-5	45.0	1
GTTCTAG	20	7.029422E-4	45.0	1
ATGATCA	20	7.029422E-4	45.0	15
GTCGAAT	25	3.8876024E-5	45.0	43
TTGTGCG	20	7.029422E-4	45.0	1
>>END_MODULE