SRR3548288_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548288_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	631957
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGC	24062	3.807537538155286	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCG	21424	3.390104073536649	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTC	19010	3.0081160585292985	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7972	1.261478233487405	No Hit
GCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	6498	1.0282345159559907	Illumina PCR Primer Index 7 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCT	5309	0.8400888035103655	Illumina PCR Primer Index 7 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTC	4653	0.7362842725058825	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCT	4303	0.6809007574882469	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGGTCATAT	3416	0.5405431065721245	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGGTCATATCGTA	3004	0.4753487974656504	No Hit
CTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCT	1684	0.26647382654199575	Illumina PCR Primer Index 7 (95% over 24bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGGTCATATCGT	1316	0.2082420164663102	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTAT	1140	0.18039202034315624	No Hit
CCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	1057	0.1672582153532598	Illumina PCR Primer Index 7 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTT	834	0.13197100435630907	No Hit
TGTACAGGGGTGTAAGAACAATTTAGGTTAAATGCCATTGTGTGTCAAACT	801	0.12674913008321767	No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCGGGTCATAT	710	0.1123494161786324	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	681	0.10776049636288544	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATG	636	0.10063975871776086	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACA	25	3.8888047E-5	45.000004	39
CAGCGCG	25	3.8888047E-5	45.000004	1
CGGAACA	25	3.8888047E-5	45.000004	37
CTGTATG	50	2.1827873E-11	45.000004	1
CTCGTAG	25	3.8888047E-5	45.000004	1
GTATTAC	25	3.8888047E-5	45.000004	34
CTACTAC	25	3.8888047E-5	45.000004	30
GGGTCCG	25	3.8888047E-5	45.000004	1
GACCGTG	25	3.8888047E-5	45.000004	12
TCGGGAA	50	2.1827873E-11	45.000004	5
GCACGAC	25	3.8888047E-5	45.000004	35
TGTATTC	25	3.8888047E-5	45.000004	11
TATTGCG	50	2.1827873E-11	45.000004	1
GTAGCTA	25	3.8888047E-5	45.000004	30
AGACGCA	25	3.8888047E-5	45.000004	38
ATGAGTA	25	3.8888047E-5	45.000004	13
TATTCGC	25	3.8888047E-5	45.000004	27
ACTACGG	25	3.8888047E-5	45.000004	2
TTGCGGA	25	3.8888047E-5	45.000004	3
CGCAGTC	25	3.8888047E-5	45.000004	32

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content