##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548288_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	631957
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.002835635968903	28.0	25.0	31.0	16.0	33.0
2	27.723680566874012	30.0	26.0	31.0	16.0	33.0
3	27.6494381737998	30.0	26.0	31.0	16.0	33.0
4	30.37544326591841	33.0	28.0	35.0	19.0	37.0
5	31.917309247306385	33.0	32.0	35.0	26.0	37.0
6	31.35776010076635	35.0	30.0	35.0	19.0	36.0
7	32.19169342217904	35.0	32.0	35.0	26.0	35.0
8	32.99023509510932	35.0	32.0	35.0	28.0	37.0
9	34.249849277719846	35.0	33.0	37.0	27.0	39.0
10	33.970214745623515	35.0	32.0	38.0	27.0	39.0
11	34.080872591014895	37.0	32.0	39.0	27.0	39.0
12	33.49962260090481	35.0	32.0	38.0	25.0	39.0
13	33.54684258580885	35.0	32.0	38.0	25.0	39.0
14	34.27089184865426	37.0	32.0	39.0	25.0	40.0
15	35.10042613658841	37.0	33.0	39.0	27.0	41.0
16	35.328308729866116	37.0	33.0	39.0	29.0	41.0
17	34.64277158097782	36.0	32.0	39.0	27.0	40.0
18	34.6007449873963	36.0	33.0	39.0	27.0	40.0
19	33.976256295918866	36.0	32.0	38.0	26.0	40.0
20	33.451494326354485	35.0	32.0	38.0	25.0	40.0
21	34.21424084233579	35.0	33.0	38.0	27.0	40.0
22	34.477727123839124	35.0	33.0	39.0	27.0	40.0
23	34.759572249377726	35.0	33.0	39.0	28.0	40.0
24	34.50720856007608	35.0	33.0	39.0	27.0	40.0
25	33.82889500393223	35.0	32.0	39.0	24.0	40.0
26	33.622543938907235	35.0	32.0	38.0	24.0	40.0
27	34.0460616782471	35.0	33.0	39.0	25.0	40.0
28	33.855012287228405	35.0	33.0	39.0	24.0	40.0
29	33.60890218796532	35.0	32.0	39.0	23.0	40.0
30	33.11543665154433	35.0	31.0	39.0	21.0	40.0
31	33.18100282139449	35.0	32.0	39.0	21.0	40.0
32	32.441768981117384	35.0	31.0	39.0	18.0	40.0
33	32.30442102864593	35.0	31.0	39.0	15.0	40.0
34	32.04947804993061	35.0	31.0	39.0	14.0	40.0
35	31.88018013883856	35.0	31.0	39.0	10.0	40.0
36	31.62782436146763	35.0	30.0	39.0	10.0	40.0
37	31.306087281254896	35.0	30.0	39.0	10.0	40.0
38	31.327031744248423	35.0	30.0	39.0	10.0	40.0
39	30.937024196266517	35.0	28.0	39.0	8.0	40.0
40	31.038241525926605	35.0	29.0	39.0	9.0	40.0
41	30.676859343278103	35.0	28.0	39.0	8.0	40.0
42	30.94911520878794	35.0	28.0	39.0	8.0	40.0
43	30.885761214766195	35.0	28.0	39.0	8.0	40.0
44	30.89686007117573	35.0	28.0	39.0	8.0	40.0
45	30.9153027816766	35.0	28.0	39.0	8.0	40.0
46	30.49149863044479	35.0	28.0	38.0	8.0	40.0
47	30.47585357864538	35.0	28.0	38.0	8.0	40.0
48	30.367066113675456	35.0	28.0	38.0	8.0	40.0
49	30.506683207876485	35.0	28.0	38.0	8.0	40.0
50	30.289198473946804	35.0	28.0	38.0	8.0	40.0
51	28.01463707182609	32.0	23.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	28.0
10	32.0
11	20.0
12	17.0
13	50.0
14	75.0
15	225.0
16	521.0
17	1149.0
18	2340.0
19	4000.0
20	5870.0
21	8298.0
22	10995.0
23	14145.0
24	17852.0
25	20675.0
26	21723.0
27	20875.0
28	21483.0
29	24081.0
30	29802.0
31	37651.0
32	47061.0
33	57292.0
34	63338.0
35	62052.0
36	66787.0
37	60297.0
38	30483.0
39	2729.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.197852069049006	17.16619959902335	20.83527834963455	23.800669982293098
2	32.51835172329763	35.105553067692895	21.522983367539247	10.853111841470227
3	20.028103177906093	33.199727196628885	36.97245223963023	9.799717385834796
4	19.129782564320042	19.875402915071753	48.86898950403271	12.125825016575495
5	28.573304829284268	22.358957967076872	35.28357150882101	13.784165694817844
6	14.754326639312485	40.07108078555978	35.11789567961111	10.056696895516627
7	61.02266451673136	3.5837881374840377	32.803497706331285	2.590049639453317
8	57.68651981068332	14.488169289999162	22.306106269888616	5.519204629428901
9	54.610361147989494	4.819315238220321	22.45690766935092	18.113415944439257
10	38.152437586734536	23.684048123527393	27.148049629958997	11.015464659779067
11	32.317705160319456	17.796622238538383	36.17619553229096	13.709477068851204
12	26.895026085635575	15.808670526633934	38.2723824564013	19.02392093132919
13	18.347292616428014	27.231916095557136	44.42833926991868	9.992452018096168
14	14.594030922990015	34.8561690114992	34.1570708133623	16.392729252148484
15	9.545427932596679	20.667228941209608	57.1311022743636	12.656240851830109
16	10.67319453697008	22.300884395615526	42.514917945366534	24.51100312204786
17	11.392705516356335	21.685019708619414	41.194574947346105	25.727699827678148
18	13.155325441446175	23.278482554983963	42.478048348226224	21.08814365534364
19	16.899092818024013	25.15281894179509	36.43808043901721	21.510007801163685
20	19.052087404681014	23.670597841308822	43.1682851839603	14.109029570049861
21	16.788009310760067	30.412195766484114	37.28845475245942	15.511340170296398
22	14.011079867775814	21.442598151456508	38.85343464824347	25.692887332524204
23	13.338565756847382	31.32728967318979	36.99523860009462	18.3389059698682
24	16.998308429212745	22.42162045835397	39.02813007847053	21.551941033962756
25	11.875016812852774	32.04838303871941	37.0279939932622	19.048606155165622
26	14.660965856854185	25.793210614013297	37.73041520230016	21.815408326832365
27	19.216497324976224	29.697906661370947	32.883882922414024	18.2017130912388
28	13.954113966614818	24.639176399660105	44.83153125924707	16.57517837447801
29	20.29821649257782	19.573167161689796	39.76488906681942	20.363727278912965
30	19.970662560902085	28.10491853084941	35.80196120938608	16.122457698862423
31	28.108874496207815	22.588245719249887	29.27841609476594	20.02446368977636
32	25.105030880265588	27.453766632856347	32.88198405904199	14.55921842783607
33	19.829830194142957	24.477931251651615	27.979435309680877	27.712803244524547
34	24.739341442534858	25.527686219157314	30.941503931438376	18.791468406869456
35	20.684793427400916	22.009725345237097	34.21482790759498	23.09065331976701
36	30.87425252034553	24.02837534832275	27.431613226849294	17.665758904482427
37	22.254520481615046	24.616864755038716	38.04451885175732	15.084095911588921
38	22.778923249524887	26.2622298669055	27.392686527722613	23.566160355846996
39	20.186974746699537	29.707717455459786	33.16633884900397	16.93896894883671
40	19.989018240165073	22.246450312283905	34.36024919416985	23.404282253381165
41	17.445016037483562	31.758964613098676	27.467216915074914	23.328802434342844
42	20.752519554336764	22.123340670330418	36.93621559694726	20.187924178385554
43	22.901716414249705	26.981424369063085	27.233340243086158	22.883518973601053
44	21.13688114855916	25.009612995820916	31.66180610389631	22.191699751723615
45	17.26573168744076	25.575948996529828	30.560307109502705	26.59801220652671
46	27.249638820362776	27.464526858631206	27.743818012934423	17.54201630807159
47	16.47010793455884	23.21518710924952	38.80343124611326	21.511273710078378
48	21.032285424482993	25.491449576474352	29.60043167493991	23.875833324102747
49	17.996635847059213	20.041711698738997	39.81156945804857	22.15008299615322
50	21.281353003448018	23.13353598425209	31.796150687467662	23.788960324832228
51	18.37229431749312	23.52580950919129	27.460729131887142	30.641167041428453
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	50.0
1	141.5
2	233.0
3	6201.5
4	12170.0
5	10091.0
6	8012.0
7	8534.0
8	9056.0
9	9614.5
10	10173.0
11	10330.5
12	10488.0
13	10426.5
14	10365.0
15	10126.5
16	9888.0
17	9310.5
18	8733.0
19	8280.0
20	7827.0
21	7209.5
22	6592.0
23	6330.0
24	6068.0
25	5822.0
26	5840.0
27	6104.0
28	6313.5
29	6523.0
30	6784.0
31	7045.0
32	7954.5
33	8864.0
34	9983.0
35	11102.0
36	12583.0
37	14064.0
38	15508.5
39	16953.0
40	21478.5
41	26004.0
42	34078.5
43	42153.0
44	45948.5
45	49744.0
46	94663.5
47	139583.0
48	101021.5
49	62460.0
50	56428.0
51	50396.0
52	40780.5
53	31165.0
54	25198.0
55	19231.0
56	16158.5
57	13086.0
58	11357.5
59	9629.0
60	7259.5
61	4890.0
62	4341.5
63	3793.0
64	2805.5
65	1818.0
66	1470.5
67	1123.0
68	739.0
69	355.0
70	287.0
71	219.0
72	257.0
73	295.0
74	189.0
75	56.5
76	30.0
77	19.0
78	8.0
79	6.5
80	5.0
81	2.5
82	0.0
83	0.5
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	631957.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.55874707866668
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.92853534748889	29.843935611042934
2	8.076260594275125	5.743634156264626
3	2.693581866665671	2.873411889973424
4	1.2407858531140108	1.7648316131867507
5	0.7171167434819197	1.2749886453675336
6	0.45692943980795586	0.9748703029756771
7	0.31139459703514816	0.7750961202345312
8	0.22876554229096638	0.6507692846910799
9	0.1735322352731461	0.5553529977666134
>10	1.4612945195801308	11.36572467281199
>50	0.3934841181920535	9.994558482598828
>100	0.3079047184930378	16.352536466303
>500	0.004075209509466677	0.9486731736225879
>1k	0.003622408452859268	3.312466598937118
>5k	0.0013584031698222255	3.184624355241203
>10k+	0.0013584031698222255	10.384525628982082
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGC	24062	3.807537538155286	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCG	21424	3.390104073536649	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTC	19010	3.0081160585292985	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7972	1.261478233487405	No Hit
GCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	6498	1.0282345159559907	Illumina PCR Primer Index 7 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCT	5309	0.8400888035103655	Illumina PCR Primer Index 7 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTC	4653	0.7362842725058825	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCT	4303	0.6809007574882469	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGGTCATAT	3416	0.5405431065721245	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGGTCATATCGTA	3004	0.4753487974656504	No Hit
CTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCT	1684	0.26647382654199575	Illumina PCR Primer Index 7 (95% over 24bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGGTCATATCGT	1316	0.2082420164663102	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTAT	1140	0.18039202034315624	No Hit
CCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	1057	0.1672582153532598	Illumina PCR Primer Index 7 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTT	834	0.13197100435630907	No Hit
TGTACAGGGGTGTAAGAACAATTTAGGTTAAATGCCATTGTGTGTCAAACT	801	0.12674913008321767	No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCGGGTCATAT	710	0.1123494161786324	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	681	0.10776049636288544	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATG	636	0.10063975871776086	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5823861433610198E-4	0.0	0.0	0.3096729682557516	0.0
2	1.5823861433610198E-4	0.0	0.0	1.8858878056576633	0.0
3	1.5823861433610198E-4	0.0	0.0	2.1821104916948464	0.0
4	1.5823861433610198E-4	0.0	0.0	3.473970539134783	0.0
5	1.5823861433610198E-4	0.0	0.0	7.666502625969805	0.0
6	1.5823861433610198E-4	0.0	0.0	8.376044572652885	0.0
7	1.5823861433610198E-4	0.0	0.0	9.584512870337697	0.0
8	1.5823861433610198E-4	0.0	0.0	11.52847424745671	0.0
9	1.5823861433610198E-4	0.0	0.0	12.089904851121199	0.0
10	1.5823861433610198E-4	0.0	0.0	16.68673659758496	0.0
11	1.5823861433610198E-4	0.0	0.0	17.73538389479031	0.0
12	1.5823861433610198E-4	0.0	0.0	23.022610715602486	0.0
13	1.5823861433610198E-4	0.0	0.0	24.01903927007692	0.0
14	1.5823861433610198E-4	0.0	0.0	24.453246027815183	0.0
15	1.5823861433610198E-4	0.0	0.0	25.290486536267498	0.0
16	1.5823861433610198E-4	0.0	0.0	26.0373727959339	0.0
17	4.7471584300830597E-4	0.0	0.0	26.68646759194059	0.0
18	6.329544573444079E-4	0.0	0.0	27.688434497916788	0.0
19	6.329544573444079E-4	0.0	0.0	28.711130662371016	0.0
20	6.329544573444079E-4	0.0	0.0	29.221766670833617	0.0
21	6.329544573444079E-4	0.0	0.0	29.585240767963644	0.0
22	6.329544573444079E-4	0.0	0.0	30.046664567367717	0.0
23	6.329544573444079E-4	0.0	0.0	30.425962525931354	0.0
24	6.329544573444079E-4	0.0	0.0	30.722976405040217	0.0
25	6.329544573444079E-4	0.0	0.0	30.95780250871499	0.0
26	6.329544573444079E-4	0.0	0.0	31.174747648969788	0.0
27	6.329544573444079E-4	0.0	0.0	31.45688709833106	0.0
28	6.329544573444079E-4	0.0	0.0	31.69788450796494	0.0
29	6.329544573444079E-4	0.0	0.0	31.9431543601859	0.0
30	7.911930716805099E-4	0.0	0.0	32.252510851212975	0.0
31	7.911930716805099E-4	0.0	0.0	32.493033545003854	0.0
32	7.911930716805099E-4	0.0	0.0	32.73925282891083	0.0
33	7.911930716805099E-4	0.0	0.0	32.96205279789606	0.0
34	0.0011076703003527139	0.0	0.0	33.23643855515486	0.0
35	0.0011076703003527139	0.0	0.0	33.49056976977864	0.0
36	0.0011076703003527139	0.0	0.0	33.70767314864777	0.0
37	0.0011076703003527139	0.0	0.0	33.95436714839776	0.0
38	0.0011076703003527139	0.0	0.0	34.16988814112353	0.0
39	0.0011076703003527139	0.0	0.0	34.41737333394519	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACA	25	3.8888047E-5	45.000004	39
CAGCGCG	25	3.8888047E-5	45.000004	1
CGGAACA	25	3.8888047E-5	45.000004	37
CTGTATG	50	2.1827873E-11	45.000004	1
CTCGTAG	25	3.8888047E-5	45.000004	1
GTATTAC	25	3.8888047E-5	45.000004	34
CTACTAC	25	3.8888047E-5	45.000004	30
GGGTCCG	25	3.8888047E-5	45.000004	1
GACCGTG	25	3.8888047E-5	45.000004	12
TCGGGAA	50	2.1827873E-11	45.000004	5
GCACGAC	25	3.8888047E-5	45.000004	35
TGTATTC	25	3.8888047E-5	45.000004	11
TATTGCG	50	2.1827873E-11	45.000004	1
GTAGCTA	25	3.8888047E-5	45.000004	30
AGACGCA	25	3.8888047E-5	45.000004	38
ATGAGTA	25	3.8888047E-5	45.000004	13
TATTCGC	25	3.8888047E-5	45.000004	27
ACTACGG	25	3.8888047E-5	45.000004	2
TTGCGGA	25	3.8888047E-5	45.000004	3
CGCAGTC	25	3.8888047E-5	45.000004	32
>>END_MODULE