SRR3548287_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548287_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	483833
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCG	9153	1.891768440763652	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGC	8608	1.7791262687745564	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8267	1.7086474051997278	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTC	7653	1.5817441141881599	No Hit
GCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	4047	0.8364456331006773	Illumina PCR Primer Index 7 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGGTCATAT	2914	0.602273925093989	No Hit
CTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCT	2849	0.5888395376090511	Illumina PCR Primer Index 7 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCT	2501	0.5169138938435369	Illumina PCR Primer Index 7 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTC	2241	0.463176343903785	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCT	2193	0.45325556545336926	No Hit
CCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	2001	0.4135724516517063	Illumina PCR Primer Index 7 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGGTCATATCGTA	1810	0.37409602073442694	No Hit
TCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	1308	0.27034121277382894	Illumina PCR Primer Index 7 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	1027	0.21226332226202013	Illumina PCR Primer Index 7 (95% over 23bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGC	810	0.16741313635076566	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGGTCATATCGT	770	0.1591458209754192	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	618	0.12773002254910268	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCG	573	0.11842929275183793	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTT	570	0.11780924409868694	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCC	547	0.11305553775786273	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTAT	493	0.10189466200114501	No Hit
GGCAGCGGGATTTAGTATTTCTCTTAAGTATATTATTTAAGCCGGGCGTGG	489	0.10106793046361037	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTCAC	20	7.029231E-4	45.000004	19
TTTAGCT	20	7.029231E-4	45.000004	3
AAGTACG	20	7.029231E-4	45.000004	1
TTAGGCG	20	7.029231E-4	45.000004	1
CCTTACG	20	7.029231E-4	45.000004	1
TCCATGC	20	7.029231E-4	45.000004	39
TGCCCGT	20	7.029231E-4	45.000004	12
CGAACCT	20	7.029231E-4	45.000004	30
GCGACTT	20	7.029231E-4	45.000004	21
CTCGTCC	20	7.029231E-4	45.000004	37
CGAAAGG	20	7.029231E-4	45.000004	2
TAAAGCG	20	7.029231E-4	45.000004	1
ACGTTAG	20	7.029231E-4	45.000004	1
ACGCACG	20	7.029231E-4	45.000004	12
TAGGTCA	20	7.029231E-4	45.000004	18
CGCATGG	20	7.029231E-4	45.000004	2
GAGTACG	20	7.029231E-4	45.000004	1
GAATCTA	20	7.029231E-4	45.000004	9
AGGACAA	20	7.029231E-4	45.000004	20
GGATCAT	40	6.8012014E-9	45.000004	8

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content