##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548287_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	483833
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.541726174113794	30.0	25.0	31.0	16.0	33.0
2	27.78398124972873	31.0	25.0	33.0	16.0	33.0
3	27.715534078907393	30.0	25.0	33.0	16.0	34.0
4	31.76713246099377	33.0	30.0	35.0	19.0	37.0
5	33.07440790520696	35.0	32.0	35.0	28.0	37.0
6	31.92029894612397	35.0	32.0	35.0	25.0	37.0
7	33.68889678876803	35.0	33.0	35.0	30.0	37.0
8	33.46338509361701	35.0	33.0	37.0	28.0	37.0
9	36.104490599029006	38.0	35.0	39.0	31.0	39.0
10	35.36620073455097	37.0	34.0	39.0	30.0	39.0
11	35.67584269779035	37.0	35.0	39.0	31.0	39.0
12	35.65809070485064	37.0	34.0	39.0	31.0	39.0
13	35.52227524786445	37.0	35.0	39.0	30.0	39.0
14	36.12286057379303	38.0	35.0	40.0	30.0	41.0
15	36.62088985249043	38.0	35.0	40.0	31.0	41.0
16	36.38882217624676	38.0	34.0	40.0	32.0	41.0
17	35.82523722028055	37.0	34.0	40.0	30.0	41.0
18	35.9515928016485	38.0	34.0	39.0	30.0	40.0
19	35.48936306535519	37.0	34.0	39.0	29.0	40.0
20	35.34369916892812	37.0	34.0	39.0	29.0	40.0
21	35.714635008360325	37.0	34.0	39.0	30.0	40.0
22	36.128736568196054	38.0	34.0	40.0	31.0	40.0
23	36.28351931348213	38.0	35.0	40.0	31.0	41.0
24	36.223647415533875	38.0	35.0	40.0	31.0	41.0
25	35.564072727573354	37.0	34.0	40.0	29.0	41.0
26	35.70623334911013	37.0	34.0	40.0	30.0	41.0
27	35.82549144022834	38.0	34.0	40.0	30.0	41.0
28	35.6716201664624	38.0	34.0	40.0	30.0	41.0
29	35.55153741063549	37.0	34.0	40.0	29.0	41.0
30	34.94780843803544	37.0	34.0	40.0	26.0	41.0
31	35.10329803878611	37.0	34.0	40.0	27.0	41.0
32	34.548937339949944	37.0	33.0	40.0	24.0	41.0
33	34.4100361074999	37.0	33.0	40.0	23.0	41.0
34	34.19442038885318	37.0	33.0	40.0	22.0	41.0
35	34.003542544638336	37.0	33.0	40.0	20.0	41.0
36	33.725727678765196	37.0	33.0	40.0	18.0	41.0
37	33.60340034681388	37.0	33.0	40.0	18.0	41.0
38	33.58385227960888	37.0	33.0	40.0	18.0	41.0
39	33.17880136328031	37.0	32.0	40.0	15.0	41.0
40	33.37850249982949	37.0	33.0	40.0	18.0	41.0
41	32.93422937253143	36.0	32.0	40.0	15.0	41.0
42	33.236701506511544	37.0	32.0	40.0	15.0	41.0
43	33.168252268861366	37.0	32.0	40.0	15.0	41.0
44	33.23376867638214	36.0	33.0	40.0	15.0	41.0
45	33.30519621439629	36.0	33.0	40.0	17.0	41.0
46	32.87031682419347	36.0	32.0	39.0	15.0	40.0
47	32.699768308486604	36.0	32.0	39.0	15.0	40.0
48	32.755086982491896	36.0	32.0	39.0	15.0	40.0
49	32.7602974580072	36.0	32.0	39.0	15.0	40.0
50	32.54409889362652	35.0	32.0	39.0	15.0	40.0
51	30.382588620453753	34.0	28.0	37.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	9.0
11	10.0
12	13.0
13	18.0
14	28.0
15	51.0
16	116.0
17	278.0
18	699.0
19	1465.0
20	2495.0
21	3621.0
22	4502.0
23	5618.0
24	6986.0
25	9326.0
26	10918.0
27	10844.0
28	10285.0
29	11307.0
30	13888.0
31	18984.0
32	26598.0
33	38706.0
34	50584.0
35	56835.0
36	71140.0
37	75275.0
38	45800.0
39	7427.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.94477226646384	21.27118241211327	24.733947457077132	17.050097864345755
2	31.297575816449065	32.09950540785767	24.178177180969467	12.4247415947238
3	22.565223951239375	30.859821467324473	34.553244611260496	12.021709970175658
4	21.094675228849624	23.322716722505493	42.0963431597266	13.486264888918283
5	26.81421068839868	26.27848865207623	32.06767624366258	14.839624415862499
6	16.636112046925284	39.55993080257031	31.486897338544495	12.317059811959911
7	67.01072477487067	4.764867216580928	24.35344426692681	3.8709637416215927
8	63.317508313819026	11.059394460485333	17.32643288076671	8.296664344928931
9	58.903795317805944	6.419983754725288	19.877313039829858	14.798907887638919
10	36.145529552552226	28.800226524441285	22.708661873001635	12.345582050004857
11	27.771772491748187	20.726573011762323	34.68924194918495	16.812412547304547
12	23.90060206724221	18.048376195918838	36.43901924837702	21.61200248846193
13	21.07400694041126	26.016828120446515	40.747737339123205	12.161427600019014
14	14.667250890286523	35.44074091680394	31.667331496611435	18.224676696298104
15	10.450093317322299	24.43632410356466	48.29579627681452	16.817786302298522
16	11.40186799990906	26.599880537292826	37.78679833744288	24.211453125355238
17	12.042791624382792	27.114314236523757	34.928787412185606	25.91410672690784
18	13.897770511726154	25.64727912316853	38.03709131043149	22.417859054673823
19	16.23514725122098	28.714039761653297	33.03247194796552	22.018341039160205
20	19.16611723466568	27.603739306744266	36.48262933698197	16.747514121608077
21	16.898599310092532	32.79995370303389	33.411114992156385	16.890331994717187
22	13.890949976541492	25.484826376042975	34.29985139500612	26.324372252409407
23	14.138556072033118	31.897782912699217	32.087311117679036	21.876349897588632
24	16.956677200604343	26.70487544255973	33.89723313622676	22.441214220609176
25	13.484611425843216	32.75386341981634	31.065264254401832	22.696260899938615
26	15.5669414860086	29.12120504388911	34.01752257493805	21.29433089516424
27	21.18044862586884	30.037016904593113	29.68338248941267	19.099151980125374
28	16.23122027641769	27.58017745792453	37.35751798657802	18.831084279079764
29	20.455611750335343	22.361847993005853	35.23054442338576	21.95199583327305
30	22.9076974906631	29.4312293704646	29.691649804788018	17.96942333408428
31	26.14063116819233	26.703015296600274	25.117550890493206	22.03880264471419
32	24.936703366657504	29.14435352694008	28.515004143991835	17.403938962410585
33	21.37287039123003	26.92747290904093	25.688202334276493	26.01145436545254
34	23.786306432178044	26.683380422583824	28.12747373577247	21.402839409465663
35	21.694882325099776	24.393127380728473	31.20766876174217	22.704321532429578
36	28.607184710426946	26.065191915392294	26.4142793071163	18.913344067064465
37	22.91265787988831	27.138496133996647	33.83584831956481	16.112997666550235
38	21.579966641382462	26.93760037037573	28.298813846926524	23.18361914131529
39	20.175763124879868	30.21063052747539	29.837154555394115	19.776451792250633
40	21.20566393776365	24.60001694799652	31.734916799804893	22.45940231443494
41	19.165290503128148	30.161233318107694	26.645350771857228	24.028125406906927
42	23.80408116023504	24.229641219180998	32.11645340437713	19.849824216206834
43	24.243075606665936	25.855822153511642	27.930711629839223	21.9703906099832
44	22.220683582971812	25.25106803380505	28.729127612213308	23.799120771009832
45	19.557367934803953	24.033499161900902	29.571567048961107	26.837565854334038
46	26.700948467756437	26.098054494009297	27.5729435569711	19.628053481263162
47	16.966597979054757	25.223372527297645	35.23963847029864	22.570391023348964
48	19.83638982872189	24.893920009590087	30.418553509165353	24.85113665252267
49	20.05919397808748	20.349790113530908	36.55910200420393	23.03191390417768
50	20.13194635339053	21.964190123451687	32.50212366663704	25.401739856520745
51	18.09467316202078	22.224403874890715	27.84638501301069	31.834537950077817
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	684.0
1	674.5
2	665.0
3	5434.5
4	10204.0
5	6766.0
6	3328.0
7	3617.5
8	3907.0
9	4363.0
10	4819.0
11	5228.0
12	5637.0
13	5730.5
14	5824.0
15	5677.0
16	5530.0
17	5371.5
18	5213.0
19	4859.0
20	4505.0
21	4173.5
22	3842.0
23	3813.5
24	3785.0
25	3835.0
26	4181.5
27	4478.0
28	4744.0
29	5010.0
30	5420.0
31	5830.0
32	6278.5
33	6727.0
34	7403.5
35	8080.0
36	8937.5
37	9795.0
38	10869.5
39	11944.0
40	14912.5
41	17881.0
42	23618.0
43	29355.0
44	33706.5
45	38058.0
46	62324.0
47	86590.0
48	77072.5
49	67555.0
50	60465.5
51	53376.0
52	42286.5
53	31197.0
54	24716.5
55	18236.0
56	14797.0
57	11358.0
58	9387.5
59	7417.0
60	5556.0
61	3695.0
62	3197.5
63	2700.0
64	2068.0
65	1436.0
66	1066.5
67	697.0
68	455.0
69	213.0
70	166.0
71	119.0
72	155.5
73	192.0
74	115.0
75	30.0
76	22.0
77	11.5
78	1.0
79	1.0
80	1.0
81	1.5
82	2.0
83	1.5
84	1.0
85	1.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	483833.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.82731260951304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.9974233886497	27.58610757872183
2	10.286923475263192	7.371056462767851
3	4.110541783455331	4.418089964109582
4	2.1272045961975654	3.048480962094525
5	1.2620544950951194	2.2608010463006987
6	0.8414641427063869	1.8088439334262563
7	0.5612707585625338	1.4076176047918891
8	0.43860769557010376	1.2571308017702585
9	0.3175171418199363	1.0238207308975749
>10	2.4255211689205325	18.69033148429777
>50	0.524866872451515	12.97056139640807
>100	0.09495371832363701	5.538686635523475
>500	0.0034952288953486016	0.8114557856249631
>1k	0.005825381492247669	4.7775294209724875
>5k	0.0023301525968990678	7.0294861922927945
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCG	9153	1.891768440763652	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGC	8608	1.7791262687745564	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8267	1.7086474051997278	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTC	7653	1.5817441141881599	No Hit
GCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	4047	0.8364456331006773	Illumina PCR Primer Index 7 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGGTCATAT	2914	0.602273925093989	No Hit
CTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCT	2849	0.5888395376090511	Illumina PCR Primer Index 7 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCT	2501	0.5169138938435369	Illumina PCR Primer Index 7 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTC	2241	0.463176343903785	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCT	2193	0.45325556545336926	No Hit
CCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	2001	0.4135724516517063	Illumina PCR Primer Index 7 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGGTCATATCGTA	1810	0.37409602073442694	No Hit
TCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	1308	0.27034121277382894	Illumina PCR Primer Index 7 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	1027	0.21226332226202013	Illumina PCR Primer Index 7 (95% over 23bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGC	810	0.16741313635076566	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGGTCATATCGT	770	0.1591458209754192	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	618	0.12773002254910268	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCG	573	0.11842929275183793	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTT	570	0.11780924409868694	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCC	547	0.11305553775786273	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTAT	493	0.10189466200114501	No Hit
GGCAGCGGGATTTAGTATTTCTCTTAAGTATATTATTTAAGCCGGGCGTGG	489	0.10106793046361037	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.652911231767986	0.0
2	0.0	0.0	0.0	2.578782348454942	0.0
3	0.0	0.0	0.0	3.3284211701144817	0.0
4	0.0	0.0	0.0	4.9076850896900375	0.0
5	0.0	0.0	0.0	8.936967920749515	0.0
6	0.0	0.0	0.0	10.669797223422131	0.0
7	0.0	0.0	0.0	12.269936114320437	0.0
8	0.0	0.0	0.0	15.489848770133497	0.0
9	0.0	0.0	0.0	16.570800255460046	0.0
10	0.0	0.0	0.0	20.727813109068624	0.0
11	0.0	0.0	0.0	23.377487686867163	0.0
12	0.0	0.0	0.0	27.779626441354765	0.0
13	0.0	0.0	0.0	28.86429821860022	0.0
14	0.0	0.0	0.0	29.33181490307606	0.0
15	0.0	0.0	0.0	30.263954711646374	0.0
16	0.0	0.0	0.0	31.484003778163128	0.0
17	0.0	0.0	0.0	32.83260959876652	0.0
18	2.066828843836613E-4	0.0	0.0	34.705569897051255	0.0
19	2.066828843836613E-4	0.0	0.0	36.27077938048872	0.0
20	2.066828843836613E-4	0.0	0.0	37.15124846796312	0.0
21	4.133657687673226E-4	0.0	0.0	37.895720217513066	0.0
22	4.133657687673226E-4	0.0	0.0	38.752007407514576	0.0
23	4.133657687673226E-4	0.0	0.0	39.518387542809194	0.0
24	4.133657687673226E-4	0.0	0.0	40.088832303708095	0.0
25	4.133657687673226E-4	0.0	0.0	40.55841581702778	0.0
26	4.133657687673226E-4	0.0	0.0	40.97736202367346	0.0
27	4.133657687673226E-4	0.0	0.0	41.47381431196301	0.0
28	4.133657687673226E-4	0.0	0.0	41.88821349515225	0.0
29	4.133657687673226E-4	0.0	0.0	42.35490344809056	0.0
30	0.0010334144219183066	0.0	0.0	42.888145289800406	0.0
31	0.0010334144219183066	0.0	0.0	43.32920656507514	0.0
32	0.0010334144219183066	0.0	0.0	43.722937459826014	0.0
33	0.0010334144219183066	0.0	0.0	44.12266215822402	0.0
34	0.0018601459594529517	0.0	0.0	44.51225939528722	0.0
35	0.0018601459594529517	0.0	0.0	44.942986526342764	0.0
36	0.0018601459594529517	0.0	0.0	45.30075459921088	0.0
37	0.0018601459594529517	0.0	0.0	45.69241866511792	0.0
38	0.0018601459594529517	0.0	0.0	46.06196766239591	0.0
39	0.0018601459594529517	0.0	0.0	46.44247085254623	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTCAC	20	7.029231E-4	45.000004	19
TTTAGCT	20	7.029231E-4	45.000004	3
AAGTACG	20	7.029231E-4	45.000004	1
TTAGGCG	20	7.029231E-4	45.000004	1
CCTTACG	20	7.029231E-4	45.000004	1
TCCATGC	20	7.029231E-4	45.000004	39
TGCCCGT	20	7.029231E-4	45.000004	12
CGAACCT	20	7.029231E-4	45.000004	30
GCGACTT	20	7.029231E-4	45.000004	21
CTCGTCC	20	7.029231E-4	45.000004	37
CGAAAGG	20	7.029231E-4	45.000004	2
TAAAGCG	20	7.029231E-4	45.000004	1
ACGTTAG	20	7.029231E-4	45.000004	1
ACGCACG	20	7.029231E-4	45.000004	12
TAGGTCA	20	7.029231E-4	45.000004	18
CGCATGG	20	7.029231E-4	45.000004	2
GAGTACG	20	7.029231E-4	45.000004	1
GAATCTA	20	7.029231E-4	45.000004	9
AGGACAA	20	7.029231E-4	45.000004	20
GGATCAT	40	6.8012014E-9	45.000004	8
>>END_MODULE