SRR3548284_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548284_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	891249
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGC	27343	3.0679417312109187	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTC	21826	2.4489228038404534	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCG	21599	2.4234529295404537	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9372	1.051557982112743	No Hit
GAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT	8178	0.9175886873365355	No Hit
GCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	7114	0.7982056641858785	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTC	5662	0.6352882303374253	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCT	4969	0.5575321823642999	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTA	4471	0.5016555418294999	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTCGCTTT	3253	0.3649933969070372	No Hit
CTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGCT	2499	0.280393021478846	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACTCGCTTTCGT	1715	0.19242658336783547	No Hit
CCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	1455	0.16325404011673506	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTAT	1360	0.15259484162114068	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATG	992	0.11130447271189084	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTT	930	0.10434794316739766	No Hit
TCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	893	0.10019646585858721	TruSeq Adapter, Index 13 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGC	35	1.2115197E-7	45.0	33
GTCATCG	25	3.890098E-5	45.0	43
AACGTCA	25	3.890098E-5	45.0	36
CGGGTAT	35	1.2115197E-7	45.0	6
CGGGTAC	70	0.0	45.0	6
TTAGGCG	20	7.0324325E-4	45.0	4
CTCACGG	50	2.1827873E-11	45.0	2
TCGTTAG	55	1.8189894E-12	45.0	1
TCTTGCG	25	3.890098E-5	45.0	1
CGAACTG	20	7.0324325E-4	45.0	28
TCCGCAA	25	3.890098E-5	45.0	33
ACTTGCG	20	7.0324325E-4	45.0	21
CGTGACG	20	7.0324325E-4	45.0	21
CGTGACA	20	7.0324325E-4	45.0	6
GACAACG	25	3.890098E-5	45.0	1
CGGCACC	20	7.0324325E-4	45.0	36
TAGCGAT	20	7.0324325E-4	45.0	34
TTCGTAC	35	1.2115197E-7	45.0	33
ATCACGC	20	7.0324325E-4	45.0	15
ACGGGTA	55	1.8189894E-12	45.0	5

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content