##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548284_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	891249
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.727957058016333	28.0	25.0	31.0	16.0	33.0
2	27.556956585645537	30.0	25.0	31.0	16.0	33.0
3	27.463905148841683	30.0	25.0	31.0	16.0	33.0
4	30.296294301592486	33.0	28.0	35.0	19.0	37.0
5	31.824840196174133	33.0	32.0	35.0	26.0	37.0
6	31.216401925836664	35.0	30.0	35.0	17.0	36.0
7	32.161366800972566	35.0	32.0	35.0	26.0	35.0
8	32.97724205020146	35.0	32.0	35.0	28.0	37.0
9	34.22395537049691	35.0	32.0	38.0	27.0	39.0
10	34.063075526592456	35.0	32.0	39.0	27.0	39.0
11	34.3148054023062	37.0	33.0	39.0	27.0	39.0
12	33.909351931951676	35.0	32.0	38.0	26.0	39.0
13	34.00910856561971	35.0	32.0	39.0	26.0	39.0
14	34.76677337085371	37.0	33.0	39.0	25.0	40.0
15	35.471862521023866	37.0	33.0	40.0	27.0	41.0
16	35.565084505003654	37.0	34.0	40.0	29.0	41.0
17	34.92076512848822	36.0	33.0	39.0	27.0	40.0
18	34.78477731812322	37.0	33.0	39.0	27.0	40.0
19	34.22111441359261	36.0	32.0	39.0	27.0	40.0
20	33.84826799244656	35.0	32.0	38.0	26.0	40.0
21	34.38168457973024	35.0	33.0	39.0	27.0	40.0
22	34.685289969469814	36.0	33.0	39.0	27.0	40.0
23	34.947832760541665	36.0	33.0	39.0	28.0	40.0
24	34.66186778330186	36.0	33.0	39.0	27.0	40.0
25	33.903364828459836	35.0	32.0	39.0	24.0	40.0
26	33.73569114804056	35.0	32.0	39.0	24.0	40.0
27	34.35224050742273	36.0	33.0	39.0	25.0	40.0
28	34.19717834185508	36.0	33.0	39.0	24.0	40.0
29	34.12031093443022	36.0	33.0	39.0	24.0	40.0
30	33.65496959884387	36.0	33.0	39.0	22.0	40.0
31	33.516193566556595	36.0	33.0	39.0	22.0	40.0
32	33.0398508161019	36.0	32.0	39.0	18.0	40.0
33	32.814422232170806	36.0	31.0	39.0	16.0	40.0
34	32.478192962909354	36.0	31.0	39.0	14.0	40.0
35	32.29926653494142	36.0	31.0	40.0	10.0	40.0
36	31.972173881822027	36.0	31.0	40.0	10.0	40.0
37	32.05079837396732	36.0	31.0	40.0	10.0	40.0
38	31.832110891569023	36.0	30.0	40.0	10.0	40.0
39	31.640455136555552	36.0	30.0	39.0	9.0	40.0
40	31.405078715375836	35.0	29.0	39.0	9.0	40.0
41	31.118642489360436	35.0	29.0	39.0	8.0	40.0
42	31.292749837587476	35.0	29.0	39.0	8.0	40.0
43	31.22150487686382	35.0	29.0	39.0	8.0	40.0
44	31.22386112074179	35.0	29.0	39.0	8.0	40.0
45	31.284832858157486	35.0	29.0	39.0	8.0	40.0
46	30.980375854559163	35.0	28.0	39.0	8.0	40.0
47	31.054651393718252	35.0	29.0	39.0	8.0	40.0
48	30.869163387560604	35.0	28.0	39.0	8.0	40.0
49	30.92957860261274	35.0	28.0	39.0	8.0	40.0
50	30.82625955260539	35.0	28.0	39.0	8.0	40.0
51	28.68099823954922	33.0	23.0	37.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	17.0
9	39.0
10	54.0
11	53.0
12	40.0
13	66.0
14	151.0
15	329.0
16	824.0
17	1697.0
18	3311.0
19	5423.0
20	8231.0
21	11457.0
22	15075.0
23	19231.0
24	23674.0
25	28016.0
26	30000.0
27	29630.0
28	28989.0
29	31293.0
30	37627.0
31	46423.0
32	58637.0
33	72533.0
34	82911.0
35	89118.0
36	101529.0
37	100321.0
38	58855.0
39	5695.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.13485232522	17.809164442260244	20.88933620121874	24.166647031301018
2	35.36710840629274	32.15083551285892	21.253207577231503	11.228848503616835
3	21.821959968538536	31.126767042655867	36.519199460532356	10.532073528273243
4	20.802491784002004	21.91351687351122	45.13654433272856	12.147447009758215
5	26.29444745520051	23.1164354742614	36.15117660721078	14.437940463327308
6	16.184029378995096	38.57608816391379	35.725930688281274	9.51395176880984
7	61.763771964961535	4.164492751184012	31.108590304168644	2.963144979685812
8	57.459363208261664	13.125400421206642	23.3411762593843	6.07406011114739
9	54.04067774550098	6.034789379847831	24.257530723737137	15.667002150914055
10	33.13866270817695	24.5145857106151	29.840594491550622	12.506157089657325
11	28.009007583739226	19.903753047689253	37.640995950626596	14.44624341794493
12	23.18824481149488	16.795194160105652	41.383496643474494	18.633064384924978
13	18.751213185091935	23.6969690849583	45.006165504814035	12.545652225135736
14	14.959006966627733	31.51835233475718	36.633421187569354	16.889219511045734
15	10.919170736797462	21.593965322822243	53.97391750229173	13.51294643808857
16	12.079789149833548	23.160194289138055	42.23567151267491	22.52434504835349
17	13.084222254386821	21.486700125329733	41.38977996048243	24.039297659801022
18	13.590926890240551	22.846926055457004	42.90159091342599	20.660556140876455
19	16.569331354088476	24.7803924604684	38.39757463963494	20.252701545808186
20	18.835476954251842	23.09444386473365	43.27197001062554	14.79810917038897
21	16.298026701853242	28.9078585221414	39.731994089193925	15.062120686811431
22	14.705318042432586	20.898200166283495	40.2434112128036	24.153070578480314
23	14.092245825801768	29.111954122809674	39.611489045149	17.184311006239557
24	16.768153456553666	21.881988086382144	38.97687402734814	22.372984429716052
25	12.53420761201415	30.738211206969098	37.60127641096933	19.12630477004743
26	12.82705506541943	25.55716752557366	39.52015654435517	22.095620864651742
27	16.412585035158525	28.220620724399133	35.31414902008305	20.052645220359295
28	10.622845018619936	24.718681311283376	46.26249230013161	18.39598136996507
29	15.411069184930362	23.96344904734816	37.63056115630985	22.994920611411626
30	16.1963716088321	24.551163591768404	38.6541808181552	20.598283981244297
31	19.217412866662404	23.56367300271866	34.977093943443414	22.241820187175527
32	20.498424121653994	26.682554482529575	32.91975643170427	19.899264964112163
33	19.725576129678686	22.734387359761413	31.61675356718493	25.92328294337497
34	18.852419469755365	24.737194656038884	34.30376920479013	22.10661666941562
35	16.085179338209638	21.50857953276806	34.44963192104563	27.956609207976673
36	20.054103847521848	24.30790946189	36.879143763415165	18.75884292717299
37	15.985543882798186	23.32423374388078	34.96261987390729	25.72760249941374
38	20.54779304100201	23.692256597202352	33.73198735706857	22.027963004727074
39	20.23721765746722	22.32170807484777	32.3306954622109	25.110378805474117
40	22.55071253936891	22.07026319244117	35.20026389931433	20.17876036887559
41	16.07126627912065	23.042494297328805	33.37204305418576	27.51419636936479
42	19.035084471343026	21.152898909283486	38.79723848217502	21.01477813719847
43	20.55766682487161	21.563446354498016	34.89305457846236	22.985832242168016
44	18.37948766281926	22.889787253618238	34.603797591918756	24.126927491643748
45	16.360411063574826	23.082999251612062	32.58909687416199	27.967492810651116
46	25.209677654617284	23.429815910031877	32.3746786812664	18.985827754084436
47	16.498083027302137	20.744258899589227	40.825066844394776	21.93259122871386
48	19.06403261041527	22.99267656962308	33.77473635314037	24.16855446682128
49	17.389640829891533	19.26251810661218	41.306301605948505	22.041539457547778
50	19.176010295663726	21.149196240332387	36.269325407377735	23.405468056626148
51	16.793286724585386	21.31873359745705	32.09787612664923	29.790103551308334
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	112.0
1	380.0
2	648.0
3	11719.0
4	22790.0
5	18813.0
6	14836.0
7	14940.5
8	15045.0
9	15346.0
10	15647.0
11	15497.0
12	15347.0
13	14985.0
14	14623.0
15	13796.5
16	12970.0
17	12251.0
18	11532.0
19	11194.0
20	10856.0
21	10086.5
22	9317.0
23	9378.0
24	9439.0
25	9785.5
26	10143.5
27	10155.0
28	10726.0
29	11297.0
30	12449.5
31	13602.0
32	15504.5
33	17407.0
34	17148.5
35	16890.0
36	18754.5
37	20619.0
38	24142.0
39	27665.0
40	31694.0
41	35723.0
42	45409.5
43	55096.0
44	60584.0
45	66072.0
46	117084.0
47	168096.0
48	125312.5
49	82529.0
50	74859.5
51	67190.0
52	52755.5
53	38321.0
54	32381.0
55	26441.0
56	23292.5
57	20144.0
58	17825.5
59	15507.0
60	13651.5
61	11796.0
62	10380.0
63	8964.0
64	7420.5
65	5877.0
66	4575.0
67	3273.0
68	2494.5
69	1716.0
70	1589.5
71	1463.0
72	1255.0
73	1047.0
74	785.0
75	381.0
76	239.0
77	208.5
78	178.0
79	120.5
80	63.0
81	44.5
82	26.0
83	23.5
84	21.0
85	14.0
86	7.0
87	6.0
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	891249.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.149750856203184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.20450488007646	28.41413111768353
2	8.074895579834145	5.515113444823848
3	2.7999649020568174	2.86854311434061
4	1.3703980465116723	1.8719500744880464
5	0.8209037270317582	1.4016828877531586
6	0.4660932790179185	0.9550181612527628
7	0.34261832264594	0.8190231249990363
8	0.2470383317414331	0.6749037984721609
9	0.192423719033868	0.5914099857447573
>10	1.5409970022147275	11.751231229866189
>50	0.6077477159207546	15.052353225791244
>100	0.32469173294479037	15.424442280359834
>500	0.0030219499446774677	0.8067855801468345
>1k	0.002350405512526919	2.2614305303660984
>5k	0.0013430888643010967	3.4773421693480535
>10k+	0.0010073166482258225	8.11463927456384
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGC	27343	3.0679417312109187	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTC	21826	2.4489228038404534	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCG	21599	2.4234529295404537	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9372	1.051557982112743	No Hit
GAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT	8178	0.9175886873365355	No Hit
GCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	7114	0.7982056641858785	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTC	5662	0.6352882303374253	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCT	4969	0.5575321823642999	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTA	4471	0.5016555418294999	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTCGCTTT	3253	0.3649933969070372	No Hit
CTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGCT	2499	0.280393021478846	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACTCGCTTTCGT	1715	0.19242658336783547	No Hit
CCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	1455	0.16325404011673506	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTAT	1360	0.15259484162114068	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATG	992	0.11130447271189084	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTT	930	0.10434794316739766	No Hit
TCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	893	0.10019646585858721	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2440417885461863E-4	0.0	0.0	0.338064895444483	0.0
2	2.2440417885461863E-4	0.0	0.0	1.7138869160021497	0.0
3	2.2440417885461863E-4	0.0	0.0	1.9957385646435508	0.0
4	2.2440417885461863E-4	0.0	0.0	3.460200235848792	0.0
5	2.2440417885461863E-4	0.0	0.0	7.226712175834138	0.0
6	2.2440417885461863E-4	0.0	0.0	8.000457784524864	0.0
7	2.2440417885461863E-4	0.0	0.0	9.130108420879013	0.0
8	2.2440417885461863E-4	0.0	0.0	11.0631260175327	0.0
9	2.2440417885461863E-4	0.0	0.0	11.607418353344576	0.0
10	2.2440417885461863E-4	0.0	0.0	15.20226109650614	0.0
11	2.2440417885461863E-4	0.0	0.0	16.34526378150214	0.0
12	2.2440417885461863E-4	0.0	0.0	20.898200166283498	0.0
13	2.2440417885461863E-4	0.0	0.0	21.941679597957474	0.0
14	2.2440417885461863E-4	0.0	0.0	22.318454214254377	0.0
15	2.2440417885461863E-4	0.0	0.0	23.17399514613761	0.0
16	2.2440417885461863E-4	0.0	0.0	23.996099855371508	0.0
17	2.2440417885461863E-4	0.0	0.0	24.584151006060036	0.0
18	2.2440417885461863E-4	0.0	0.0	25.855512881360877	0.0
19	2.2440417885461863E-4	0.0	0.0	26.626116831547638	0.0
20	2.2440417885461863E-4	0.0	0.0	27.220787905512378	0.0
21	2.2440417885461863E-4	0.0	0.0	27.599469957329546	0.0
22	2.2440417885461863E-4	0.0	0.0	27.984771932422927	0.0
23	2.2440417885461863E-4	0.0	0.0	28.33405703681014	0.0
24	2.2440417885461863E-4	0.0	0.0	28.58707274846872	0.0
25	2.2440417885461863E-4	0.0	0.0	28.79812487868149	0.0
26	2.2440417885461863E-4	0.0	0.0	29.02163144082069	0.0
27	2.2440417885461863E-4	0.0	0.0	29.24356717370791	0.0
28	4.4880835770923726E-4	0.0	0.0	29.447999380644465	0.0
29	4.4880835770923726E-4	0.0	0.0	29.66185656309292	0.0
30	4.4880835770923726E-4	0.0	0.0	29.91947256041802	0.0
31	4.4880835770923726E-4	0.0	0.0	30.15419933149995	0.0
32	4.4880835770923726E-4	0.0	0.0	30.37860351035457	0.0
33	4.4880835770923726E-4	0.0	0.0	30.601997870404343	0.0
34	4.4880835770923726E-4	0.0	0.0	30.82808508060037	0.0
35	4.4880835770923726E-4	0.0	0.0	31.074929677340453	0.0
36	4.4880835770923726E-4	0.0	0.0	31.30471955648758	0.0
37	4.4880835770923726E-4	0.0	0.0	31.549656717707396	0.0
38	4.4880835770923726E-4	0.0	0.0	31.766094548212678	0.0
39	4.4880835770923726E-4	0.0	0.0	31.995884427359805	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGC	35	1.2115197E-7	45.0	33
GTCATCG	25	3.890098E-5	45.0	43
AACGTCA	25	3.890098E-5	45.0	36
CGGGTAT	35	1.2115197E-7	45.0	6
CGGGTAC	70	0.0	45.0	6
TTAGGCG	20	7.0324325E-4	45.0	4
CTCACGG	50	2.1827873E-11	45.0	2
TCGTTAG	55	1.8189894E-12	45.0	1
TCTTGCG	25	3.890098E-5	45.0	1
CGAACTG	20	7.0324325E-4	45.0	28
TCCGCAA	25	3.890098E-5	45.0	33
ACTTGCG	20	7.0324325E-4	45.0	21
CGTGACG	20	7.0324325E-4	45.0	21
CGTGACA	20	7.0324325E-4	45.0	6
GACAACG	25	3.890098E-5	45.0	1
CGGCACC	20	7.0324325E-4	45.0	36
TAGCGAT	20	7.0324325E-4	45.0	34
TTCGTAC	35	1.2115197E-7	45.0	33
ATCACGC	20	7.0324325E-4	45.0	15
ACGGGTA	55	1.8189894E-12	45.0	5
>>END_MODULE