FastQCFastQC Report
Sat 18 Jun 2016
SRR3548282_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548282_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993428
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGC393133.957307424393112No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCG306383.084068498169973No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTC300583.025684800508945No Hit
GAACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCT94570.9519562565178352No Hit
GCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC90340.9093764218443611No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87420.8799832499184642No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTC74650.7514384535165105No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCT65040.6547027061850482No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAAAGAGT46980.47290795105432903No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTA43360.43646847079003204No Hit
CTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGCT19290.19417612549676475No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGT18440.18561989394299336No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTAT14720.14817379820178211No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTT11370.1144521797251537No Hit
TTCGTAGGGGAAGACATACAGGAAGGTGAAGAAAAAGCTGTTAGAGAAGAT10950.11022439472211373No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATG10850.10921777924519946No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTT10400.10468800959908518No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCTA253.8904214E-545.00000444
CGCATTG253.8904214E-545.00000428
CGCATAC253.8904214E-545.00000440
ACTCTAG253.8904214E-545.00000411
TTACTAG253.8904214E-545.0000041
GTCTACC253.8904214E-545.0000046
ATTCCGA253.8904214E-545.00000444
GTTCGCC253.8904214E-545.00000443
AGTCTGC650.045.00000424
GTTCCAC253.8904214E-545.00000438
CCGGTCT502.1827873E-1145.00000418
CACGTTG253.8904214E-545.00000431
CAATCCG253.8904214E-545.0000041
TAGTACG253.8904214E-545.0000041
TCGTTAC207.032824E-445.024
GTACCGC207.032824E-445.031
AGTCCCG406.8139343E-945.037
ACACGTA207.032824E-445.023
TACCCGC207.032824E-445.028
AGTTTCG207.032824E-445.037