SRR3548280_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548280_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	705126
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGC	25915	3.6752296752637115	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTC	21182	3.004002121606635	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG	19097	2.7083102878067185	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT	7695	1.0912943218658793	No Hit
GCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	5975	0.8473662863091135	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTC	5071	0.7191622490164878	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4872	0.6909403425770714	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT	3993	0.5662817709175382	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTA	3697	0.5243034578217226	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCGAGAAT	3263	0.4627541744312364	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTT	3019	0.4281504298522533	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2717	0.3853212050044956	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTT	1703	0.24151711892626285	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGT	1516	0.21499703599073072	No Hit
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCT	1414	0.20053153620771322	No Hit
GTGAATGATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGC	1158	0.16422596812484577	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTAT	1157	0.16408414949952208	No Hit
GTGAATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT	1034	0.14664045858470684	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATG	827	0.11728400314270074	No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCTCCGAGAAT	725	0.10281850335968323	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	712	0.10097486123047511	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTAGT	25	3.8892653E-5	45.000004	23
GTCGCTC	25	3.8892653E-5	45.000004	33
TCGTTGT	25	3.8892653E-5	45.000004	41
TCGTTAG	30	2.1643027E-6	45.000004	1
ACGCCTA	25	3.8892653E-5	45.000004	36
TCCGCGT	25	3.8892653E-5	45.000004	16
GCGACAG	30	2.1643027E-6	45.000004	1
ATCTACG	25	3.8892653E-5	45.000004	1
GTCGAAC	25	3.8892653E-5	45.000004	39
TAGACAC	25	3.8892653E-5	45.000004	11
AACTTGC	25	3.8892653E-5	45.000004	28
CGGCACG	25	3.8892653E-5	45.000004	35
TAGCGCA	30	2.1643027E-6	45.000004	30
TAGCGAG	25	3.8892653E-5	45.000004	1
GGCAACG	25	3.8892653E-5	45.000004	1
TAAGTCA	25	3.8892653E-5	45.000004	35
TCTAGTC	25	3.8892653E-5	45.000004	19
ATCAATC	25	3.8892653E-5	45.000004	35
CACCTCG	25	3.8892653E-5	45.000004	43
CACTCTA	25	3.8892653E-5	45.000004	42

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content