##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548280_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	705126
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.7998258467281	28.0	25.0	31.0	16.0	33.0
2	27.701093137963994	30.0	26.0	31.0	16.0	33.0
3	27.731813888581616	30.0	26.0	31.0	16.0	33.0
4	30.716144348669598	33.0	28.0	35.0	19.0	37.0
5	32.0000879275477	33.0	32.0	35.0	26.0	37.0
6	31.223249461798318	35.0	30.0	35.0	17.0	37.0
7	32.099359547088035	35.0	32.0	35.0	26.0	35.0
8	32.891773952456724	35.0	32.0	35.0	28.0	37.0
9	34.15614372466765	35.0	32.0	37.0	28.0	39.0
10	33.790158071039784	35.0	32.0	38.0	27.0	39.0
11	34.094398731574216	37.0	32.0	39.0	27.0	39.0
12	33.63162044797667	35.0	32.0	38.0	25.0	39.0
13	33.543119953029674	35.0	32.0	38.0	25.0	39.0
14	34.50152596840848	37.0	33.0	39.0	25.0	40.0
15	35.100237404378795	37.0	33.0	39.0	27.0	41.0
16	35.273877292852625	37.0	33.0	39.0	28.0	41.0
17	34.705289551087326	36.0	33.0	39.0	27.0	40.0
18	34.541167110558966	36.0	33.0	39.0	27.0	40.0
19	33.93124633044307	36.0	32.0	38.0	25.0	40.0
20	33.522951926322385	35.0	32.0	38.0	25.0	40.0
21	34.34203674236945	35.0	33.0	39.0	27.0	40.0
22	34.644428088029656	36.0	33.0	39.0	27.0	40.0
23	34.959282454483315	36.0	33.0	39.0	28.0	40.0
24	34.78440590759666	36.0	33.0	39.0	27.0	40.0
25	34.087740347115265	35.0	33.0	39.0	25.0	40.0
26	33.88432563825472	35.0	32.0	39.0	24.0	40.0
27	34.38662735454373	36.0	33.0	39.0	25.0	40.0
28	34.099190782923905	36.0	33.0	39.0	24.0	40.0
29	34.08761980128374	36.0	33.0	39.0	24.0	40.0
30	33.5698768730695	36.0	32.0	39.0	23.0	40.0
31	33.646205075404964	36.0	33.0	39.0	23.0	40.0
32	33.06873665132189	35.0	32.0	39.0	20.0	40.0
33	32.783096921684916	35.0	31.0	39.0	18.0	40.0
34	32.541907403783156	35.0	31.0	39.0	15.0	40.0
35	32.5106902879769	36.0	31.0	39.0	15.0	40.0
36	32.26037474153556	36.0	31.0	39.0	12.0	40.0
37	32.19266627524726	36.0	31.0	39.0	10.0	40.0
38	32.00977981240232	35.0	30.0	39.0	10.0	40.0
39	31.658978678987868	35.0	30.0	39.0	10.0	40.0
40	31.086705071150405	35.0	29.0	39.0	10.0	40.0
41	30.918311053627296	35.0	28.0	39.0	9.0	40.0
42	31.007405768614404	35.0	29.0	39.0	10.0	40.0
43	31.03548727461475	35.0	29.0	39.0	10.0	40.0
44	31.168846418937893	35.0	29.0	39.0	10.0	40.0
45	31.301592906799637	35.0	30.0	39.0	10.0	40.0
46	30.859840652592585	35.0	28.0	38.0	9.0	40.0
47	30.657778893417632	35.0	28.0	38.0	9.0	40.0
48	30.637776794501974	35.0	27.0	38.0	8.0	40.0
49	30.669592384907094	35.0	28.0	38.0	8.0	40.0
50	30.52201592339525	35.0	27.0	38.0	8.0	40.0
51	27.911581759855686	32.0	22.0	36.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	13.0
9	33.0
10	50.0
11	36.0
12	28.0
13	34.0
14	77.0
15	198.0
16	479.0
17	1073.0
18	2116.0
19	3543.0
20	5468.0
21	7903.0
22	10676.0
23	13554.0
24	17226.0
25	21244.0
26	23469.0
27	24382.0
28	25505.0
29	28759.0
30	34315.0
31	42498.0
32	52342.0
33	63313.0
34	71062.0
35	70256.0
36	75206.0
37	68488.0
38	37868.0
39	3911.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.30180138017886	17.35689791611712	19.462337227672784	20.878963476031235
2	32.10149675377167	34.90255642254009	21.780929933089972	11.215016890598276
3	22.757776624319625	33.707167229686604	33.06132520996247	10.473730936031291
4	20.393660140173527	21.014400263215368	46.48814538110919	12.10379421550191
5	28.085051465979127	24.014147826062292	32.929575707036754	14.97122500092182
6	15.92764981010486	41.10045580506179	33.512024801241196	9.459869583592152
7	62.4920936116382	3.962412391544206	30.749823435811475	2.795670561006118
8	57.92638478796698	15.298542388168922	20.514205971698676	6.260866852165428
9	54.2520060244552	4.469697614327084	21.772988090071845	19.505308271145864
10	37.98711152333058	20.229575990674007	27.65193738424055	14.131375101754864
11	32.018958313833274	20.296798019077443	31.702560960736097	15.98168270635319
12	26.484628279201168	14.547612767079926	37.868125696684	21.099633257034913
13	20.325870837268802	24.410956339718005	42.75746462334391	12.50570819966928
14	16.044791994622237	32.36130847536469	33.29972231913161	18.29417721088146
15	10.59555313518435	20.243757853206375	54.48898494737111	14.671704064238163
16	12.315813060360844	21.750013472769407	40.16317651029745	25.7709969565723
17	13.030720750617618	21.31151028326852	39.139813309961625	26.517955656152232
18	15.429015523466727	21.660242282939503	38.69904102245556	24.21170117113821
19	17.872408619168773	24.77784112343042	33.99988087235472	23.349869385046077
20	20.553631549538665	21.589332970277653	41.14966686804912	16.70736861213457
21	19.1033942869785	28.690758814736654	36.0198035528402	16.186043345444645
22	15.285778711889789	20.548809716277656	38.358818140304	25.80659343152855
23	15.205509369956575	29.17407668983983	35.505143761540495	20.115270178663106
24	18.520945192774057	20.29084163681385	36.419873894878364	24.768339275533734
25	14.34623031912027	31.092457234593535	34.18552145290345	20.375790993382743
26	14.911944815536515	23.329872958875438	36.43036847315231	25.327813752435734
27	19.183805447537036	27.542027949614678	30.50022265524176	22.773943947606526
28	13.81129046439927	22.339269860989383	43.7387076919586	20.110731982652748
29	18.21220604544436	19.460068129667604	38.62940807742162	23.69831774746641
30	15.752078351954118	25.010565487586618	35.181655477177124	24.055700683282137
31	24.139940946724415	22.092647271551467	27.796308744820074	25.97110303690404
32	25.574294523248327	29.21364408630514	27.85502165570409	17.357039734742443
33	19.979975210104293	26.29671860064726	25.93153564043873	27.79177054880972
34	25.286544532466536	23.99216593913712	31.25611592821708	19.46517360017926
35	18.14938039442596	27.54429704761986	26.78329830413288	27.523024253821305
36	20.97043648936502	25.956212081245056	31.11500639602	21.958345033369923
37	21.77695901158091	23.728808751911007	31.848350507568863	22.645881728939223
38	18.558668947110164	29.729154789356794	23.74965608983359	27.962520173699453
39	29.864307939290285	23.45694244716547	27.53947521435885	19.139274399185393
40	20.199226804854735	29.565637914358568	29.335040829582233	20.900094451204467
41	21.396317821212097	28.162342616780546	26.79592016178669	23.645419400220668
42	21.235211862844373	27.508984209914257	31.458491106554003	19.797312820687367
43	22.53171773555365	27.781275970535763	26.735363608773465	22.951642685137124
44	20.542144240887446	24.191421107716916	30.362942225928414	24.90349242546722
45	19.04723411135031	24.01017690455323	28.01272396706404	28.92986501703242
46	28.120222485059408	26.551283033103303	26.06527060411898	19.263223877718307
47	17.495029257182402	22.72459106599388	38.17544665776046	21.60493301906326
48	21.54664556405522	23.9944350371423	28.899657649838467	25.559261748964012
49	18.70233121456307	19.611104965637345	38.62884080292033	23.057723016879255
50	20.834148790428944	21.5920275241588	33.458417360868836	24.115406324543414
51	18.377282925321147	21.719806105575458	28.533056503376702	31.369854465726693
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	73.0
1	257.0
2	441.0
3	6907.0
4	13373.0
5	11122.5
6	8872.0
7	9052.5
8	9233.0
9	9521.0
10	9809.0
11	9710.5
12	9612.0
13	9240.0
14	8868.0
15	8342.0
16	7816.0
17	7412.0
18	7008.0
19	6506.5
20	6005.0
21	5706.0
22	5407.0
23	5306.0
24	5205.0
25	4699.0
26	4621.5
27	5050.0
28	5444.5
29	5839.0
30	6569.0
31	7299.0
32	8204.5
33	9110.0
34	9128.0
35	9146.0
36	10718.0
37	12290.0
38	14935.5
39	17581.0
40	20532.0
41	23483.0
42	29703.5
43	35924.0
44	44798.5
45	53673.0
46	103196.0
47	152719.0
48	113648.0
49	74577.0
50	66824.0
51	59071.0
52	46233.0
53	33395.0
54	29643.5
55	25892.0
56	22972.5
57	20053.0
58	18815.5
59	17578.0
60	16863.0
61	16148.0
62	14232.5
63	12317.0
64	9902.0
65	7487.0
66	5782.5
67	4078.0
68	3428.5
69	2779.0
70	2200.5
71	1622.0
72	1215.0
73	808.0
74	728.0
75	443.5
76	239.0
77	221.5
78	204.0
79	155.0
80	106.0
81	79.5
82	53.0
83	37.5
84	22.0
85	15.5
86	9.0
87	9.0
88	9.0
89	5.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	705126.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.18678534095055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.8944060844354	27.00291239946258
2	7.814630156306842	5.030556467199345
3	2.652676263327785	2.5614336440029875
4	1.2362933080666718	1.5916922930078248
5	0.7009092690111993	1.1280008092573017
6	0.4682160842627462	0.9042222358407258
7	0.31168056651510917	0.7022396842557375
8	0.22185429161633044	0.5712621168978779
9	0.17120423658513423	0.4959462611184326
>10	1.5380801936286337	11.184835924824496
>50	0.5838294884090418	13.644713504780068
>100	0.3950355848771064	18.04972243101649
>500	0.0031316524282443314	0.6479860144523583
>1k	0.005368547019847425	4.254100183325782
>5k	0.0013421367549618563	2.6986457548559213
>10k+	0.0013421367549618563	9.53173027570209
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGC	25915	3.6752296752637115	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTC	21182	3.004002121606635	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG	19097	2.7083102878067185	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT	7695	1.0912943218658793	No Hit
GCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	5975	0.8473662863091135	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTC	5071	0.7191622490164878	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4872	0.6909403425770714	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT	3993	0.5662817709175382	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTA	3697	0.5243034578217226	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCGAGAAT	3263	0.4627541744312364	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTT	3019	0.4281504298522533	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2717	0.3853212050044956	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTT	1703	0.24151711892626285	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGT	1516	0.21499703599073072	No Hit
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCT	1414	0.20053153620771322	No Hit
GTGAATGATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGC	1158	0.16422596812484577	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTAT	1157	0.16408414949952208	No Hit
GTGAATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT	1034	0.14664045858470684	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATG	827	0.11728400314270074	No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCTCCGAGAAT	725	0.10281850335968323	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	712	0.10097486123047511	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2636408244767602	0.0
2	0.0	0.0	0.0	1.6446705978789606	0.0
3	0.0	0.0	0.0	1.8823586139214836	0.0
4	0.0	0.0	0.0	3.576382093413092	0.0
5	0.0	0.0	0.0	8.245193057694653	0.0
6	0.0	0.0	0.0	9.326134619911903	0.0
7	0.0	0.0	0.0	10.629589605262039	0.0
8	0.0	0.0	0.0	12.599450311008246	0.0
9	0.0	0.0	0.0	13.084611828240627	0.0
10	0.0	0.0	0.0	17.146297257511424	0.0
11	0.0	0.0	0.0	18.095489316802954	0.0
12	0.0	0.0	0.0	23.600037440117084	0.0
13	0.0	0.0	0.0	24.641269787243697	0.0
14	0.0	0.0	0.0	25.237333469479214	0.0
15	0.0	0.0	0.0	26.121714416997815	0.0
16	0.0	0.0	0.0	26.838749386634447	0.0
17	0.0	0.0	0.0	27.352558266182214	0.0
18	0.0	0.0	0.0	28.27281932590771	0.0
19	0.0	0.0	0.0	29.09508371553453	0.0
20	0.0	0.0	0.0	29.57457248775396	0.0
21	0.0	0.0	0.0	29.88189344883042	0.0
22	0.0	0.0	0.0	30.23445455138514	0.0
23	0.0	0.0	0.0	30.491004444595717	0.0
24	0.0	0.0	0.0	30.725146995005147	0.0
25	0.0	0.0	0.0	30.895471164018915	0.0
26	0.0	0.0	0.0	31.127344616423166	0.0
27	0.0	0.0	0.0	31.322912500744547	0.0
28	0.0	0.0	0.0	31.48543664536551	0.0
29	0.0	0.0	0.0	31.650797162492946	0.0
30	0.0	0.0	0.0	31.85983781622008	0.0
31	0.0	0.0	0.0	32.061929357306354	0.0
32	0.0	0.0	0.0	32.23352989394803	0.0
33	0.0	0.0	0.0	32.433635974279774	0.0
34	0.0	0.0	0.0	32.67118217169698	0.0
35	0.0	0.0	0.0	32.871997345155336	0.0
36	0.0	0.0	0.0	33.0590561119573	0.0
37	0.0	0.0	0.0	33.2415766827489	0.0
38	0.0	0.0	0.0	33.413177219390576	0.0
39	0.0	0.0	0.0	33.60406508907628	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTAGT	25	3.8892653E-5	45.000004	23
GTCGCTC	25	3.8892653E-5	45.000004	33
TCGTTGT	25	3.8892653E-5	45.000004	41
TCGTTAG	30	2.1643027E-6	45.000004	1
ACGCCTA	25	3.8892653E-5	45.000004	36
TCCGCGT	25	3.8892653E-5	45.000004	16
GCGACAG	30	2.1643027E-6	45.000004	1
ATCTACG	25	3.8892653E-5	45.000004	1
GTCGAAC	25	3.8892653E-5	45.000004	39
TAGACAC	25	3.8892653E-5	45.000004	11
AACTTGC	25	3.8892653E-5	45.000004	28
CGGCACG	25	3.8892653E-5	45.000004	35
TAGCGCA	30	2.1643027E-6	45.000004	30
TAGCGAG	25	3.8892653E-5	45.000004	1
GGCAACG	25	3.8892653E-5	45.000004	1
TAAGTCA	25	3.8892653E-5	45.000004	35
TCTAGTC	25	3.8892653E-5	45.000004	19
ATCAATC	25	3.8892653E-5	45.000004	35
CACCTCG	25	3.8892653E-5	45.000004	43
CACTCTA	25	3.8892653E-5	45.000004	42
>>END_MODULE