SRR3548279_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548279_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	540694
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTC	15487	2.864281830388353	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGC	14021	2.5931488050542453	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG	11400	2.108401424835489	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5712	1.056420082338624	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT	5411	1.0007508868232309	No Hit
GCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	5166	0.9554387509386084	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTC	3974	0.7349813387979153	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTT	3794	0.7016907899847233	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCGAGAAT	3568	0.6598926564748268	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT	3125	0.5779609168956933	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTA	2785	0.5150787691374419	No Hit
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCT	2318	0.4287082897165495	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1401	0.2591114382626772	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	1348	0.24930922111212625	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGC	1110	0.20529171768135027	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGT	1085	0.20066803034618472	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	781	0.14444399235057168	No Hit
ACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	780	0.14425904485716504	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG	728	0.1346417752000207	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATG	726	0.13427188021320746	No Hit
TCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	680	0.12576429551650287	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTAT	679	0.12557934802309625	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTAT	45	3.8380676E-10	45.000004	37
GCTCTAG	45	3.8380676E-10	45.000004	1
AGGGCCG	45	3.8380676E-10	45.000004	6
TCTGCGA	20	7.02997E-4	45.0	4
CTTCGGC	40	6.8030204E-9	45.0	38
GTCATCG	25	3.888055E-5	45.0	43
AGCGTCC	20	7.02997E-4	45.0	28
GAGTGGT	20	7.02997E-4	45.0	16
AGCCGAT	20	7.02997E-4	45.0	38
GTACCGG	25	3.888055E-5	45.0	2
ACACGTG	20	7.02997E-4	45.0	42
ATTAGTC	20	7.02997E-4	45.0	44
GTTCTAC	20	7.02997E-4	45.0	14
CGTATAG	20	7.02997E-4	45.0	1
AAGAGTG	20	7.02997E-4	45.0	1
CAAGTGA	20	7.02997E-4	45.0	11
TGCTTCG	40	6.8030204E-9	45.0	36
CGCGGTA	20	7.02997E-4	45.0	30
ACTTGCA	20	7.02997E-4	45.0	1
ACGCACA	20	7.02997E-4	45.0	20

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content