##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548279_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	540694
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.305290977891378	30.0	25.0	31.0	16.0	33.0
2	27.763520586505493	30.0	25.0	31.0	16.0	33.0
3	27.73247345078732	30.0	25.0	31.0	16.0	34.0
4	31.965547980928214	35.0	32.0	35.0	19.0	37.0
5	33.09503526948699	35.0	32.0	35.0	28.0	37.0
6	31.875521089562675	35.0	32.0	35.0	25.0	37.0
7	33.587698402423555	35.0	33.0	35.0	30.0	37.0
8	33.11777641327627	35.0	33.0	36.0	28.0	37.0
9	35.70872434315898	37.0	35.0	39.0	30.0	39.0
10	35.152883146474714	37.0	34.0	39.0	30.0	39.0
11	35.633970415798956	37.0	35.0	39.0	31.0	39.0
12	35.69682667090813	37.0	35.0	39.0	31.0	39.0
13	35.47082638239004	37.0	34.0	39.0	30.0	39.0
14	36.190747446799854	38.0	35.0	40.0	30.0	41.0
15	36.54204041472626	38.0	35.0	40.0	31.0	41.0
16	36.30698879588085	38.0	34.0	40.0	31.0	41.0
17	35.77954443733424	37.0	34.0	40.0	30.0	41.0
18	35.83723880790244	37.0	34.0	39.0	30.0	40.0
19	35.37278941508506	37.0	34.0	39.0	29.0	40.0
20	35.205931636008536	37.0	34.0	39.0	29.0	40.0
21	35.656889109181904	37.0	34.0	39.0	30.0	40.0
22	36.121447621020394	38.0	34.0	40.0	31.0	41.0
23	36.25859358528114	38.0	35.0	40.0	31.0	41.0
24	36.277541455980646	38.0	35.0	40.0	31.0	41.0
25	35.62177497808372	37.0	34.0	40.0	29.0	41.0
26	35.76384054566908	37.0	34.0	40.0	30.0	41.0
27	35.924739316508045	38.0	35.0	40.0	30.0	41.0
28	35.70651976903757	38.0	35.0	40.0	29.0	41.0
29	35.72644786145214	38.0	35.0	40.0	29.0	41.0
30	35.10916525798326	37.0	34.0	40.0	26.0	41.0
31	35.31778233159606	38.0	34.0	40.0	27.0	41.0
32	34.849944700699474	37.0	34.0	40.0	25.0	41.0
33	34.536345881404266	37.0	34.0	40.0	23.0	41.0
34	34.36968229719582	38.0	34.0	40.0	22.0	41.0
35	34.2621260824052	38.0	34.0	40.0	21.0	41.0
36	33.994531102619966	38.0	33.0	40.0	19.0	41.0
37	34.14520227707354	38.0	33.0	40.0	20.0	41.0
38	33.91986595005678	37.0	33.0	40.0	20.0	41.0
39	33.659365556118615	37.0	33.0	40.0	18.0	41.0
40	33.263727727698104	37.0	32.0	40.0	15.0	41.0
41	32.94056157456898	37.0	31.0	40.0	15.0	41.0
42	33.19807136753876	37.0	32.0	40.0	15.0	41.0
43	33.32261500959878	37.0	33.0	40.0	15.0	41.0
44	33.618407084228785	37.0	33.0	40.0	18.0	41.0
45	33.7834079904715	37.0	33.0	40.0	18.0	41.0
46	33.38691015620665	37.0	33.0	40.0	17.0	41.0
47	33.2119831179928	37.0	32.0	40.0	18.0	41.0
48	33.3087069580946	37.0	33.0	40.0	18.0	41.0
49	33.34105242521648	37.0	33.0	40.0	17.0	41.0
50	33.18704664745679	37.0	32.0	40.0	16.0	41.0
51	30.939139328344684	34.0	28.0	38.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	13.0
10	34.0
11	28.0
12	22.0
13	32.0
14	38.0
15	57.0
16	147.0
17	291.0
18	699.0
19	1470.0
20	2557.0
21	3907.0
22	5212.0
23	6238.0
24	7367.0
25	9325.0
26	11042.0
27	11978.0
28	11797.0
29	13105.0
30	15837.0
31	21224.0
32	29192.0
33	41715.0
34	54581.0
35	61166.0
36	78215.0
37	85746.0
38	57448.0
39	10211.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.807588025759486	19.917920302426143	20.741676438059233	15.532815233755137
2	30.61472847858493	35.21344790214058	21.722822890581366	12.449000728693123
3	23.476679970556358	34.566686517697626	29.945403499946366	12.01123001179965
4	21.040551587404334	22.72764262225954	43.187459080367084	13.04434670996904
5	28.147713864034003	25.708996215974285	29.885295564589214	16.257994355402502
6	16.72517172374763	41.223131752895355	30.218016105227726	11.833680418129292
7	64.11038406196481	5.050916044934843	26.76153240095137	4.077167492148979
8	58.586002433909016	14.056379393890076	17.72259355568954	9.635024616511373
9	53.888890943861036	6.103822124898741	20.779960569194404	19.227326362045815
10	36.67286857261223	22.565258723048526	24.800534128360958	15.961338575978278
11	29.276078521307802	22.163367819875937	30.21394726037278	18.346606398443484
12	23.70324064997947	15.130924330582548	37.00688374570459	24.158951273733386
13	22.700640288222175	23.057219055510142	40.63647830380955	13.605662352458136
14	15.463090028740842	33.56223668100626	31.245214483608102	19.729458806644793
15	10.449163482487322	21.82972254177039	50.11226312849782	17.60885084724447
16	11.338575978279767	24.649616973741153	36.899059357048536	27.112747690930544
17	11.892863616019413	25.340025966628076	34.63104824540313	28.136062171949384
18	14.830199706303379	22.92091275286946	35.933633441465965	26.31525409936119
19	16.803404513458627	27.271987482753644	31.232268159069644	24.692339844718084
20	19.933640839365705	24.010253489034465	36.76552726680895	19.290578404790878
21	18.044402194217064	31.549083215275182	33.302940295250174	17.103574295257577
22	14.245950574631863	22.946250559466165	34.89459842350757	27.913200442394405
23	14.973718961186918	30.03158903187385	32.34805638679179	22.64663562014744
24	18.22121199791379	22.86931240220901	32.519132818192915	26.39034278168428
25	14.216543923180208	32.3735791408819	29.72106219044413	23.688814745493755
26	14.21876329310109	25.068338098813747	34.542088501074545	26.170810107010617
27	19.97969276522395	27.061147340270097	27.793354466666916	25.16580542783904
28	14.203782546135152	24.161170643654266	38.45872156894657	23.176325241264006
29	17.700954698960967	20.565791371829537	35.82469936784947	25.90855456136003
30	17.405593551990588	25.757822354233635	30.947818914210256	25.88876517956552
31	22.443563272386967	24.10679608059272	23.899654887977302	29.549985759043008
32	24.757996204877436	29.598257054822135	24.58562514102246	21.058121599277964
33	20.43040980665589	27.246834623650347	22.84970796790791	29.47304760178585
34	24.53975816265762	24.001191061857536	28.20412284952302	23.25492792596182
35	19.9279074670701	27.684235445557004	24.312272745767476	28.07558434160542
36	19.166663584208443	28.45324712314174	28.66353242314507	23.71655686950475
37	22.774804233078232	23.83196410539048	28.684616437393423	24.708615224137866
38	19.93696989424702	29.459916329753984	21.750565014592357	28.852548761406638
39	30.18842450628267	24.409185232312545	24.707505539177426	20.69488472222736
40	22.819376578989225	28.19025178751752	28.25239414530215	20.737977488191103
41	21.571535840974747	28.021949568517496	24.733398188254355	25.6731164022534
42	22.610940753919962	26.839025400688744	29.82796184163316	20.722072003758132
43	22.624256973445238	26.03801780674467	26.877864374304135	24.45986084550596
44	21.101399312735115	22.83805627582329	28.171387143190046	27.88915726825154
45	20.52399323831964	20.688966402438346	27.1645329890844	31.62250737015761
46	28.524266960609886	25.029314177704947	25.17949154235113	21.26692731933404
47	17.973197409255512	22.185931414071543	35.94657976600443	23.89429141066851
48	20.79346173621309	22.916104118040888	28.30824828831095	27.982185857435077
49	20.43040980665589	18.575016552800662	36.466282222477034	24.528291418066413
50	20.358465231720714	19.258582488431532	34.03570226412722	26.347250015720537
51	18.51546345992373	19.21881877734911	27.719930311784484	34.545787450942676
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	863.0
1	850.0
2	837.0
3	5030.5
4	9224.0
5	6365.5
6	3507.0
7	3518.0
8	3529.0
9	3725.0
10	3921.0
11	3997.5
12	4074.0
13	4115.0
14	4156.0
15	3946.0
16	3736.0
17	3597.0
18	3458.0
19	3346.5
20	3235.0
21	3076.0
22	2917.0
23	2999.5
24	3082.0
25	2958.5
26	3349.0
27	3863.0
28	4242.5
29	4622.0
30	5055.0
31	5488.0
32	5951.0
33	6414.0
34	6386.5
35	6359.0
36	7237.0
37	8115.0
38	9753.5
39	11392.0
40	13455.0
41	15518.0
42	20178.0
43	24838.0
44	31575.0
45	38312.0
46	75715.0
47	113118.0
48	94199.0
49	75280.0
50	67571.5
51	59863.0
52	46826.0
53	33789.0
54	28856.5
55	23924.0
56	20247.5
57	16571.0
58	14878.0
59	13185.0
60	12250.5
61	11316.0
62	9694.5
63	8073.0
64	6457.5
65	4842.0
66	3739.5
67	2637.0
68	2149.0
69	1661.0
70	1334.5
71	1008.0
72	733.0
73	458.0
74	392.5
75	234.5
76	142.0
77	122.0
78	102.0
79	73.5
80	45.0
81	42.0
82	39.0
83	23.5
84	8.0
85	4.5
86	1.0
87	4.0
88	7.0
89	4.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	540694.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.6462814942614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.56168877436279	24.545390361237796
2	9.684315206030652	6.129451301784041
3	3.8754624655360113	3.679319283143908
4	2.001942970866335	2.5341620316597595
5	1.1956854073575423	1.8919498489908697
6	0.7959546787417683	1.5113403492081838
7	0.5770979520981123	1.278410296930105
8	0.4224298684279898	1.0694667622284775
9	0.30476039482338824	0.8680079918594871
>10	2.765185938820755	19.905446865919295
>50	0.7040338058307921	15.410182700451042
>100	0.09729110382083811	4.916166248015149
>500	0.004717144427676999	1.014544746468472
>1k	0.005896430534596249	4.573195263279256
>5k	0.0017689291603788747	3.0395290371942147
>10k+	0.0017689291603788747	7.633436911629992
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTC	15487	2.864281830388353	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGC	14021	2.5931488050542453	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG	11400	2.108401424835489	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5712	1.056420082338624	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT	5411	1.0007508868232309	No Hit
GCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	5166	0.9554387509386084	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTC	3974	0.7349813387979153	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTT	3794	0.7016907899847233	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCGAGAAT	3568	0.6598926564748268	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT	3125	0.5779609168956933	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTA	2785	0.5150787691374419	No Hit
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCT	2318	0.4287082897165495	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1401	0.2591114382626772	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	1348	0.24930922111212625	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGC	1110	0.20529171768135027	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGT	1085	0.20066803034618472	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	781	0.14444399235057168	No Hit
ACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	780	0.14425904485716504	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG	728	0.1346417752000207	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATG	726	0.13427188021320746	No Hit
TCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	680	0.12576429551650287	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTAT	679	0.12557934802309625	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4797537978967771	0.0
2	0.0	0.0	0.0	2.181825579717918	0.0
3	0.0	0.0	0.0	2.7473950145553676	0.0
4	0.0	0.0	0.0	4.8175123082556865	0.0
5	0.0	0.0	0.0	10.036730572190555	0.0
6	0.0	0.0	0.0	12.32767517301838	0.0
7	0.0	0.0	0.0	14.133872393627449	0.0
8	0.0	0.0	0.0	17.5204459453961	0.0
9	0.0	0.0	0.0	18.494749340662185	0.0
10	0.0	0.0	0.0	22.945880664479354	0.0
11	0.0	0.0	0.0	25.359445453435768	0.0
12	1.8494749340662185E-4	0.0	0.0	30.874949601808048	0.0
13	1.8494749340662185E-4	0.0	0.0	32.27426233692255	0.0
14	1.8494749340662185E-4	0.0	0.0	32.92250330131276	0.0
15	1.8494749340662185E-4	0.0	0.0	34.04716900871843	0.0
16	1.8494749340662185E-4	0.0	0.0	35.19106925543838	0.0
17	1.8494749340662185E-4	0.0	0.0	36.20846541666821	0.0
18	1.8494749340662185E-4	0.0	0.0	37.95326006946628	0.0
19	1.8494749340662185E-4	0.0	0.0	39.39363114811705	0.0
20	1.8494749340662185E-4	0.0	0.0	40.314114822801805	0.0
21	1.8494749340662185E-4	0.0	0.0	40.98861833125576	0.0
22	1.8494749340662185E-4	0.0	0.0	41.699186600923994	0.0
23	1.8494749340662185E-4	0.0	0.0	42.25365918615705	0.0
24	1.8494749340662185E-4	0.0	0.0	42.72490539935712	0.0
25	1.8494749340662185E-4	0.0	0.0	43.07852500675058	0.0
26	1.8494749340662185E-4	0.0	0.0	43.496691289342955	0.0
27	1.8494749340662185E-4	0.0	0.0	43.84309794449356	0.0
28	1.8494749340662185E-4	0.0	0.0	44.14086340887822	0.0
29	1.8494749340662185E-4	0.0	0.0	44.46489141732662	0.0
30	1.8494749340662185E-4	0.0	0.0	44.83941009147503	0.0
31	1.8494749340662185E-4	0.0	0.0	45.205421180926734	0.0
32	1.8494749340662185E-4	0.0	0.0	45.507440437659746	0.0
33	3.698949868132437E-4	0.0	0.0	45.860875097559806	0.0
34	3.698949868132437E-4	0.0	0.0	46.2181936548214	0.0
35	3.698949868132437E-4	0.0	0.0	46.57236810469508	0.0
36	3.698949868132437E-4	0.0	0.0	46.89177242580831	0.0
37	3.698949868132437E-4	0.0	0.0	47.20488853214572	0.0
38	3.698949868132437E-4	0.0	0.0	47.488967882018294	0.0
39	3.698949868132437E-4	0.0	0.0	47.79524093109966	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTAT	45	3.8380676E-10	45.000004	37
GCTCTAG	45	3.8380676E-10	45.000004	1
AGGGCCG	45	3.8380676E-10	45.000004	6
TCTGCGA	20	7.02997E-4	45.0	4
CTTCGGC	40	6.8030204E-9	45.0	38
GTCATCG	25	3.888055E-5	45.0	43
AGCGTCC	20	7.02997E-4	45.0	28
GAGTGGT	20	7.02997E-4	45.0	16
AGCCGAT	20	7.02997E-4	45.0	38
GTACCGG	25	3.888055E-5	45.0	2
ACACGTG	20	7.02997E-4	45.0	42
ATTAGTC	20	7.02997E-4	45.0	44
GTTCTAC	20	7.02997E-4	45.0	14
CGTATAG	20	7.02997E-4	45.0	1
AAGAGTG	20	7.02997E-4	45.0	1
CAAGTGA	20	7.02997E-4	45.0	11
TGCTTCG	40	6.8030204E-9	45.0	36
CGCGGTA	20	7.02997E-4	45.0	30
ACTTGCA	20	7.02997E-4	45.0	1
ACGCACA	20	7.02997E-4	45.0	20
>>END_MODULE