SRR3548278_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548278_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	713213
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGC	31949	4.479587444424036	No Hit
GAATCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTC	26751	3.7507729107573753	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCG	26022	3.648559406516707	No Hit
GAACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCT	8817	1.2362365800959882	No Hit
GCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC	7893	1.1066820150502024	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTC	6503	0.9117893252085982	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5620	0.7879833934602987	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCT	5288	0.7414334848074838	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTA	4150	0.581873858160185	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATGGTTGT	3898	0.5465407949658797	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTT	2744	0.3847377992268789	No Hit
CTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGCT	1627	0.22812259451243877	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCATGGTTGTCGT	1599	0.22419669860196043	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTAT	1434	0.20106195484378442	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1017	0.14259414789130317	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATG	961	0.13474235607034646	No Hit
TCTAACGGGATATAGCTCACTGACTTTGACATATTCTAACTATAAATGCCA	926	0.1298349861822485	No Hit
TGTGACGGGGGAAAAACTTTTGCTGGATATTTGTTAAATATCAATAGCCCT	762	0.10684045299230384	No Hit
CCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC	758	0.10627961071937836	TruSeq Adapter, Index 22 (95% over 22bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGG	20	7.031484E-4	45.0	2
AACGTAG	20	7.031484E-4	45.0	1
CGGGTAT	20	7.031484E-4	45.0	6
CGGAAAT	20	7.031484E-4	45.0	24
ACTATAC	20	7.031484E-4	45.0	25
GCGACTT	20	7.031484E-4	45.0	20
CGAGTTA	20	7.031484E-4	45.0	29
GTCGACT	20	7.031484E-4	45.0	10
CTCAACC	20	7.031484E-4	45.0	36
CGAGTGA	20	7.031484E-4	45.0	36
AACCCGA	20	7.031484E-4	45.0	25
GCGAAAA	20	7.031484E-4	45.0	34
CGGTTTA	20	7.031484E-4	45.0	43
ACCGCAC	35	1.211065E-7	45.0	28
ATGTGCG	20	7.031484E-4	45.0	1
TTAGACG	40	6.8084773E-9	45.0	1
CGTTGAT	20	7.031484E-4	45.0	25
CCAATGC	20	7.031484E-4	45.0	41
CCTACGT	20	7.031484E-4	45.0	14
CGATTCA	20	7.031484E-4	45.0	45

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content