##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548278_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	713213
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.82202371521551	28.0	25.0	31.0	16.0	33.0
2	27.726819337280727	30.0	26.0	31.0	16.0	33.0
3	27.64499104755522	30.0	26.0	31.0	16.0	33.0
4	30.71220939607102	33.0	28.0	35.0	19.0	37.0
5	31.992448258795058	33.0	32.0	35.0	26.0	37.0
6	31.356943858286375	35.0	30.0	35.0	19.0	37.0
7	32.17151257758902	35.0	32.0	35.0	26.0	35.0
8	32.882467089074375	35.0	32.0	35.0	28.0	37.0
9	34.15616372668474	35.0	32.0	37.0	28.0	39.0
10	33.88982393758947	35.0	32.0	38.0	27.0	39.0
11	34.157023217467994	37.0	32.0	39.0	27.0	39.0
12	33.771023523127035	35.0	32.0	38.0	26.0	39.0
13	33.68839463105692	35.0	32.0	38.0	25.0	39.0
14	34.553950923496906	37.0	32.0	39.0	25.0	40.0
15	35.06269094926761	37.0	33.0	39.0	27.0	41.0
16	35.319375838634464	37.0	33.0	39.0	29.0	41.0
17	34.639355984817996	36.0	32.0	39.0	27.0	40.0
18	34.5609516371687	36.0	33.0	39.0	27.0	40.0
19	34.01963368586944	36.0	32.0	38.0	26.0	40.0
20	33.44696745572501	35.0	31.0	38.0	25.0	40.0
21	34.295761574732936	35.0	33.0	39.0	27.0	40.0
22	34.63385131790923	36.0	33.0	39.0	27.0	40.0
23	34.875113044770636	36.0	33.0	39.0	28.0	40.0
24	34.66756494904047	36.0	33.0	39.0	27.0	40.0
25	33.994238747751375	35.0	33.0	39.0	24.0	40.0
26	33.84233041181246	35.0	32.0	39.0	24.0	40.0
27	34.28473401354154	36.0	33.0	39.0	25.0	40.0
28	34.04370363411772	36.0	33.0	39.0	24.0	40.0
29	33.93852467635896	36.0	33.0	39.0	24.0	40.0
30	33.513298271343906	36.0	32.0	39.0	23.0	40.0
31	33.4740407143448	36.0	32.0	39.0	22.0	40.0
32	33.02668347324011	36.0	31.0	39.0	19.0	40.0
33	32.86893957345141	36.0	31.0	39.0	17.0	40.0
34	32.49875563120695	36.0	31.0	39.0	15.0	40.0
35	32.43228881133687	36.0	31.0	39.0	13.0	40.0
36	32.20753267256766	36.0	31.0	39.0	10.0	40.0
37	32.042040736778496	36.0	30.0	39.0	10.0	40.0
38	32.08113705162413	36.0	30.0	39.0	10.0	40.0
39	31.877725167656788	35.0	30.0	39.0	10.0	40.0
40	31.739093370423703	35.0	30.0	39.0	10.0	40.0
41	31.10866178827363	35.0	29.0	39.0	9.0	40.0
42	31.65990524569799	35.0	30.0	39.0	10.0	40.0
43	31.751954885847567	35.0	30.0	39.0	10.0	40.0
44	31.693663744211054	35.0	30.0	39.0	10.0	40.0
45	31.798839897758455	35.0	30.0	39.0	10.0	40.0
46	31.496171550434443	35.0	30.0	39.0	10.0	40.0
47	31.54948101058169	35.0	30.0	39.0	10.0	40.0
48	31.407137839607522	35.0	30.0	39.0	10.0	40.0
49	31.518884260382244	35.0	30.0	39.0	10.0	40.0
50	31.284799912508607	35.0	29.0	39.0	10.0	40.0
51	29.133759479987045	33.0	24.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	18.0
9	21.0
10	45.0
11	39.0
12	38.0
13	39.0
14	104.0
15	217.0
16	513.0
17	1155.0
18	2218.0
19	3872.0
20	5777.0
21	8148.0
22	10764.0
23	14158.0
24	17484.0
25	21011.0
26	23135.0
27	22695.0
28	23145.0
29	25849.0
30	31864.0
31	40233.0
32	50678.0
33	62959.0
34	70516.0
35	72608.0
36	80530.0
37	76081.0
38	43027.0
39	4272.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.448591094105126	16.125477241721615	18.631180306584426	21.794751357588826
2	31.17189395033461	36.9282388290735	20.372735774586275	11.527131446005612
3	20.29449827751317	36.79938531686887	33.330856279961246	9.57526012565671
4	19.669860196042414	19.606064387497142	48.60483474081375	12.119240675646687
5	30.1255024796239	21.908321917856235	33.423956097266874	14.54221950525299
6	15.443212616707772	41.389318478491	32.898727308672164	10.268741596129066
7	59.903422960602235	3.6014486555909664	33.7624244089774	2.732703974829399
8	55.6142414678364	16.379959423061553	21.608131091272874	6.397668017829177
9	52.50016474741768	4.5550207301325125	21.791246093383045	21.153568429066773
10	36.637862742266336	22.01852742448609	27.48673958550952	13.856870247738055
11	31.537422901713796	19.336439464788217	34.51633663435748	14.60980099914051
12	25.304502301556475	15.473918731150441	38.536313836119085	20.685265131173995
13	18.876408590421097	24.21464555469404	44.93468290678941	11.97426294809545
14	16.03980858453225	32.92396521095381	33.20242339946131	17.833802805052628
15	10.328050666490936	20.591744682163675	54.72236204331665	14.357842608028736
16	10.972178016945849	22.50029093692908	40.19220064693157	26.335330399193506
17	11.867422495103146	21.561861603756522	40.99757015085255	25.573145750287786
18	13.4154873789457	22.27707571230474	41.07047964633286	23.236957262416695
19	16.734271528982227	25.03025043009592	35.78299890775967	22.452479133162186
20	19.620506076024974	21.718617019039193	43.155691217069794	15.505185687866039
21	16.9049077905198	30.67400622254502	36.977172317386255	15.443913669548929
22	13.212041844441982	20.03076219866996	40.08774377359919	26.66945218328886
23	14.650041432222913	30.310159797984614	36.45909426777134	18.580704502021135
24	18.21811997257481	20.821409592926656	36.809059846076835	24.151410588421694
25	12.69073895175775	32.43701390748626	34.01424258952094	20.858004551235044
26	13.803590231810132	23.071929423608374	36.82406237687759	26.300417967703897
27	17.976817584648625	29.582747370000266	31.870983843536223	20.569451201814886
28	12.168174163959435	24.06560172066409	43.79603288218246	19.970191233194008
29	17.789215844355052	20.464012854504894	35.40386953126205	26.342901769878
30	15.40507534214884	30.194766500330196	38.01515115400308	16.38500700351788
31	23.447273114763753	20.500748023381515	34.898130011651496	21.153848850203236
32	27.65387058284131	26.844855604146307	30.187615761350393	15.313658051661985
33	24.10514110090534	21.976744675153146	29.122996916769605	24.79511730717191
34	21.256342775580368	21.10757936268688	36.17348534028404	21.462592521448713
35	19.24754596452953	25.028848324413605	33.002202708026914	22.72140300302995
36	25.79678160661682	19.060785487645347	33.312348484954704	21.830084420783134
37	20.402320204483093	27.157945803006957	36.00565329011109	16.434080702398862
38	22.74089227201411	24.4102392973768	32.105275703050836	20.743592727558248
39	28.02472753581328	20.88422392749431	33.757096407384616	17.333952129307796
40	25.319364621789003	20.780047475298403	35.27109012314694	18.629497779765654
41	20.35170418935157	23.39890046872393	34.32999678917798	21.919398552746514
42	20.09918495596687	20.616281531604162	39.46801306201654	19.81652045041243
43	28.535795057016628	20.28734753853337	27.816234420853235	23.360622983596766
44	21.29265731275229	23.207232621951647	33.54860329242456	21.951506772871497
45	17.3026851725922	22.640221084023988	32.17552119773476	27.881572545649057
46	28.596786654197277	26.424924952293356	28.132970094487902	16.845318299021468
47	17.81038764015799	20.843703073275446	41.590380433334786	19.755528853231784
48	21.00901133322023	23.503637763192764	30.726024343358855	24.76132656022815
49	18.00163485522558	17.810247429589758	41.60944907061425	22.578668644570417
50	21.817886101347003	20.650913541957312	33.84767243446207	23.683527922233612
51	18.84107552722679	21.004524595036827	28.393200909125326	31.76119896861106
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	50.0
1	236.0
2	422.0
3	7477.5
4	14533.0
5	12181.0
6	9829.0
7	9873.0
8	9917.0
9	10227.0
10	10537.0
11	10378.0
12	10219.0
13	9698.5
14	9178.0
15	8804.5
16	8431.0
17	7844.5
18	7258.0
19	7447.0
20	7636.0
21	6999.0
22	6362.0
23	5745.5
24	5129.0
25	5532.5
26	5793.0
27	5650.0
28	6400.0
29	7150.0
30	8540.0
31	9930.0
32	10304.0
33	10678.0
34	13298.0
35	15918.0
36	16556.0
37	17194.0
38	18255.5
39	19317.0
40	23217.5
41	27118.0
42	33741.0
43	40364.0
44	45678.5
45	50993.0
46	110113.5
47	169234.0
48	116932.0
49	64630.0
50	58861.5
51	53093.0
52	41236.5
53	29380.0
54	26430.5
55	23481.0
56	19983.0
57	16485.0
58	15065.5
59	13646.0
60	12963.5
61	12281.0
62	10627.5
63	8974.0
64	6974.0
65	4974.0
66	4054.0
67	3134.0
68	2291.5
69	1449.0
70	1231.5
71	1014.0
72	894.0
73	774.0
74	582.0
75	289.5
76	189.0
77	155.0
78	121.0
79	118.5
80	116.0
81	89.5
82	63.0
83	41.5
84	20.0
85	15.0
86	10.0
87	6.5
88	3.0
89	3.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	713213.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.757632029125915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.43231058306905	26.81370250865981
2	7.608695294190829	4.832682907493086
3	2.470576860161607	2.353790124740568
4	1.152519941425427	1.4640521682407386
5	0.64500970261493	1.024199039543044
6	0.42346364568411127	0.8068921582408896
7	0.2988635370114765	0.6643838764733467
8	0.2150637574980086	0.5463932538738342
9	0.16212571685150226	0.463385597240544
>10	1.5989634273823952	11.778518415734121
>50	0.650832620742144	14.944472048688109
>100	0.3321490616198784	14.360896266616638
>500	0.0026931004996206356	0.6586965532277436
>1k	0.003141950582890742	2.347559734929173
>5k	0.0022442504163505297	4.863749208544436
>10k+	0.0013465502498103178	12.076626137753927
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGC	31949	4.479587444424036	No Hit
GAATCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTC	26751	3.7507729107573753	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCG	26022	3.648559406516707	No Hit
GAACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCT	8817	1.2362365800959882	No Hit
GCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC	7893	1.1066820150502024	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTC	6503	0.9117893252085982	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5620	0.7879833934602987	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCT	5288	0.7414334848074838	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTA	4150	0.581873858160185	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATGGTTGT	3898	0.5465407949658797	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTT	2744	0.3847377992268789	No Hit
CTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGCT	1627	0.22812259451243877	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCATGGTTGTCGT	1599	0.22419669860196043	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTAT	1434	0.20106195484378442	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1017	0.14259414789130317	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATG	961	0.13474235607034646	No Hit
TCTAACGGGATATAGCTCACTGACTTTGACATATTCTAACTATAAATGCCA	926	0.1298349861822485	No Hit
TGTGACGGGGGAAAAACTTTTGCTGGATATTTGTTAAATATCAATAGCCCT	762	0.10684045299230384	No Hit
CCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC	758	0.10627961071937836	TruSeq Adapter, Index 22 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2675217641854537	0.0
2	0.0	0.0	0.0	1.8563879233833371	0.0
3	0.0	0.0	0.0	2.089137466647411	0.0
4	0.0	0.0	0.0	3.7474078571198226	0.0
5	0.0	0.0	0.0	8.626875842139725	0.0
6	0.0	0.0	0.0	9.525345163366344	0.0
7	0.0	0.0	0.0	10.717134993332987	0.0
8	0.0	0.0	0.0	12.58137470853728	0.0
9	0.0	0.0	0.0	13.010699468461736	0.0
10	0.0	0.0	0.0	17.675645284087643	0.0
11	0.0	0.0	0.0	18.478771418916928	0.0
12	0.0	0.0	0.0	24.30662368745382	0.0
13	0.0	0.0	0.0	25.230471121530314	0.0
14	0.0	0.0	0.0	25.66400219850171	0.0
15	0.0	0.0	0.0	26.585746474054737	0.0
16	0.0	0.0	0.0	27.275582469753076	0.0
17	0.0	0.0	0.0	27.736454607529588	0.0
18	4.2063170469410963E-4	0.0	0.0	28.555985378841946	0.0
19	4.2063170469410963E-4	0.0	0.0	29.49189092178634	0.0
20	4.2063170469410963E-4	0.0	0.0	29.902848097272482	0.0
21	4.2063170469410963E-4	0.0	0.0	30.17794123214243	0.0
22	4.2063170469410963E-4	0.0	0.0	30.524401546242146	0.0
23	4.2063170469410963E-4	0.0	0.0	30.78771699338066	0.0
24	4.2063170469410963E-4	0.0	0.0	30.996069897772475	0.0
25	4.2063170469410963E-4	0.0	0.0	31.15422741873746	0.0
26	4.2063170469410963E-4	0.0	0.0	31.356691479263556	0.0
27	4.2063170469410963E-4	0.0	0.0	31.57107343808932	0.0
28	4.2063170469410963E-4	0.0	0.0	31.729932011895464	0.0
29	4.2063170469410963E-4	0.0	0.0	31.91248617173271	0.0
30	4.2063170469410963E-4	0.0	0.0	32.137383923175825	0.0
31	4.2063170469410963E-4	0.0	0.0	32.34447493245356	0.0
32	4.2063170469410963E-4	0.0	0.0	32.52758993456373	0.0
33	4.2063170469410963E-4	0.0	0.0	32.70509651394464	0.0
34	7.010528411568493E-4	0.0	0.0	32.93616353038994	0.0
35	7.010528411568493E-4	0.0	0.0	33.141852433985356	0.0
36	7.010528411568493E-4	0.0	0.0	33.31851774995688	0.0
37	7.010528411568493E-4	0.0	0.0	33.509484543888014	0.0
38	8.412634093882193E-4	0.0	0.0	33.68502817531368	0.0
39	8.412634093882193E-4	0.0	0.0	33.88048170742822	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGG	20	7.031484E-4	45.0	2
AACGTAG	20	7.031484E-4	45.0	1
CGGGTAT	20	7.031484E-4	45.0	6
CGGAAAT	20	7.031484E-4	45.0	24
ACTATAC	20	7.031484E-4	45.0	25
GCGACTT	20	7.031484E-4	45.0	20
CGAGTTA	20	7.031484E-4	45.0	29
GTCGACT	20	7.031484E-4	45.0	10
CTCAACC	20	7.031484E-4	45.0	36
CGAGTGA	20	7.031484E-4	45.0	36
AACCCGA	20	7.031484E-4	45.0	25
GCGAAAA	20	7.031484E-4	45.0	34
CGGTTTA	20	7.031484E-4	45.0	43
ACCGCAC	35	1.211065E-7	45.0	28
ATGTGCG	20	7.031484E-4	45.0	1
TTAGACG	40	6.8084773E-9	45.0	1
CGTTGAT	20	7.031484E-4	45.0	25
CCAATGC	20	7.031484E-4	45.0	41
CCTACGT	20	7.031484E-4	45.0	14
CGATTCA	20	7.031484E-4	45.0	45
>>END_MODULE