SRR3548277_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548277_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	510981
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGC	16816	3.290924711486337	No Hit
GAATCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTC	16293	3.1885725692344726	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCG	14760	2.8885614142208813	No Hit
GCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC	6049	1.1838013546491943	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5736	1.1225466308923424	No Hit
GAACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCT	5527	1.0816449143901632	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTC	4281	0.8378002313197556	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATGGTTGT	4234	0.828602237656586	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCT	3724	0.7287942213115556	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTA	3043	0.595521164192015	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTT	2792	0.5463999639908333	No Hit
CTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGCT	2268	0.44385211974613537	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGC	1297	0.2538254847049108	No Hit
CCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC	1282	0.25088995481240983	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCATGGTTGTCGT	1139	0.22290456983723464	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCG	991	0.1939406748978925	No Hit
TCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC	969	0.18963523105555785	TruSeq Adapter, Index 22 (95% over 22bp)
TGTGACGGGGGAAAAACTTTTGCTGGATATTTGTTAAATATCAATAGCCCT	819	0.16027993213054886	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTAT	806	0.15773580622371478	No Hit
ACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC	784	0.15343036238138014	TruSeq Adapter, Index 23 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATG	775	0.15166904444587959	No Hit
TCTAACGGGATATAGCTCACTGACTTTGACATATTCTAACTATAAATGCCA	649	0.12701059334887208	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	569	0.11135443392220064	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	554	0.10841890402969974	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTCG	20	7.0296024E-4	45.000004	43
ACAACGA	40	6.8030204E-9	45.000004	13
AGGTATA	30	2.1631258E-6	45.000004	39
CGGGTGA	70	0.0	45.000004	6
CGAACGG	35	1.2101555E-7	45.000004	2
GGCACCC	20	7.0296024E-4	45.000004	23
CTATACG	20	7.0296024E-4	45.000004	17
CCAATTG	20	7.0296024E-4	45.000004	24
ATAGCGG	35	1.2101555E-7	45.000004	2
CTCCCAC	20	7.0296024E-4	45.000004	40
GAATCGT	20	7.0296024E-4	45.000004	22
GATCACG	20	7.0296024E-4	45.000004	1
TCTAGCC	20	7.0296024E-4	45.000004	40
TCAGGTA	20	7.0296024E-4	45.000004	44
CCGTCGA	40	6.8030204E-9	45.000004	41
TATTGTC	20	7.0296024E-4	45.000004	35
GTAATGG	65	0.0	45.000004	2
TGTGACA	20	7.0296024E-4	45.000004	42
TTGAGCG	20	7.0296024E-4	45.000004	1
TACGCGG	20	7.0296024E-4	45.000004	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content