SRR3548270_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548270_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	639149
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGC	17723	2.772905848245088	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCG	14610	2.28585196878975	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTC	13451	2.1045171000815146	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5466	0.8551996482823254	No Hit
GCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	4694	0.7344140411703688	TruSeq Adapter, Index 21 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCT	4536	0.7096936708028957	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	3408	0.5332090013439745	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTC	3317	0.5189713196766325	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCT	2916	0.4562316455161472	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTA	2066	0.32324231126075453	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGCGGTTT	2036	0.3185485700517407	No Hit
CTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGCT	953	0.14910451240634032	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGT	853	0.1334587083762941	No Hit
CCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	850	0.13298933425539272	TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTAT	740	0.1157789498223419	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCAAG	70	0.0	45.000004	1
AACGTAG	25	3.8888542E-5	45.000004	1
CTAGACC	25	3.8888542E-5	45.000004	25
CGAACTT	25	3.8888542E-5	45.000004	12
CTCAAAT	25	3.8888542E-5	45.000004	40
GGGTCTA	25	3.8888542E-5	45.000004	7
TAGACGG	25	3.8888542E-5	45.000004	2
CGTGAAT	25	3.8888542E-5	45.000004	27
GTTAGCG	25	3.8888542E-5	45.000004	1
CACGATG	25	3.8888542E-5	45.000004	42
TAGCACG	50	2.1827873E-11	45.000004	1
AGAGTAG	25	3.8888542E-5	45.000004	1
ACCTATG	25	3.8888542E-5	45.000004	34
ACTAGCC	25	3.8888542E-5	45.000004	22
CATAGTT	25	3.8888542E-5	45.000004	18
GTTCGGT	25	3.8888542E-5	45.000004	29
ACGTGTG	25	3.8888542E-5	45.000004	37
AGCGCTA	35	1.2107921E-7	45.000004	19
CGAGCTT	25	3.8888542E-5	45.000004	17
TACTCAC	25	3.8888542E-5	45.000004	30