##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548270_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	639149
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.758702587346612	28.0	25.0	31.0	16.0	33.0
2	27.602476104945794	30.0	25.0	31.0	16.0	33.0
3	27.593927237623777	30.0	25.0	31.0	16.0	33.0
4	30.50767035542573	33.0	28.0	35.0	19.0	37.0
5	31.862551611596043	33.0	32.0	35.0	26.0	37.0
6	31.098003751863807	35.0	30.0	35.0	17.0	37.0
7	32.00185872151877	33.0	31.0	35.0	26.0	35.0
8	33.08431523791792	35.0	32.0	35.0	28.0	37.0
9	34.37541324479894	37.0	33.0	38.0	28.0	39.0
10	33.93380260314888	35.0	32.0	39.0	27.0	39.0
11	34.27216345484386	37.0	32.0	39.0	27.0	39.0
12	33.7126648089882	35.0	32.0	38.0	25.0	39.0
13	33.757834245222945	35.0	32.0	39.0	25.0	39.0
14	34.58977797039501	37.0	33.0	39.0	25.0	40.0
15	35.360426129118565	37.0	33.0	40.0	27.0	41.0
16	35.379903590555564	37.0	33.0	40.0	27.0	41.0
17	34.88605160924917	36.0	33.0	39.0	27.0	40.0
18	34.719678822934874	36.0	33.0	39.0	27.0	40.0
19	34.146213167821585	36.0	32.0	38.0	26.0	40.0
20	33.936096278019676	35.0	32.0	38.0	27.0	40.0
21	34.45711719802425	35.0	33.0	39.0	27.0	40.0
22	34.66622962720743	36.0	33.0	39.0	27.0	40.0
23	35.05824307008225	36.0	33.0	39.0	29.0	40.0
24	34.82210251443716	36.0	33.0	39.0	27.0	40.0
25	34.109423624225336	35.0	33.0	39.0	25.0	40.0
26	34.042138843994124	35.0	33.0	39.0	24.0	40.0
27	34.47932015852329	36.0	33.0	39.0	26.0	40.0
28	34.33293019311616	36.0	33.0	39.0	25.0	40.0
29	34.314117678350435	36.0	33.0	39.0	25.0	40.0
30	33.87062641105595	36.0	33.0	39.0	23.0	40.0
31	33.813900983964615	36.0	33.0	39.0	23.0	40.0
32	33.2666623901469	36.0	32.0	39.0	20.0	40.0
33	33.15041875994486	36.0	32.0	39.0	18.0	40.0
34	32.76727179421387	36.0	32.0	39.0	15.0	40.0
35	32.4444378384383	36.0	31.0	39.0	13.0	40.0
36	32.216221882534434	36.0	31.0	39.0	10.0	40.0
37	32.21932288089319	36.0	31.0	39.0	10.0	40.0
38	32.09337728761212	35.0	31.0	39.0	10.0	40.0
39	31.932981198437297	35.0	31.0	39.0	10.0	40.0
40	31.625361222500544	35.0	30.0	39.0	10.0	40.0
41	31.409361510383338	35.0	30.0	39.0	10.0	40.0
42	31.33773658411419	35.0	30.0	39.0	10.0	40.0
43	31.348435184909935	35.0	30.0	39.0	10.0	40.0
44	31.430355050230855	35.0	30.0	39.0	10.0	40.0
45	31.487036669070907	35.0	30.0	39.0	10.0	40.0
46	31.259994148469293	35.0	29.0	39.0	10.0	40.0
47	31.307014483320792	35.0	29.0	39.0	10.0	40.0
48	31.19690087913773	35.0	29.0	39.0	10.0	40.0
49	31.220357068539574	35.0	29.0	39.0	10.0	40.0
50	31.173091094564803	35.0	29.0	39.0	10.0	40.0
51	29.055262544414525	33.0	24.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	5.0
8	21.0
9	47.0
10	34.0
11	48.0
12	49.0
13	41.0
14	78.0
15	183.0
16	429.0
17	877.0
18	1852.0
19	3144.0
20	4632.0
21	6656.0
22	9198.0
23	12275.0
24	15726.0
25	19275.0
26	21057.0
27	21222.0
28	21502.0
29	23801.0
30	28430.0
31	35930.0
32	44858.0
33	55558.0
34	63213.0
35	63775.0
36	71067.0
37	69038.0
38	40917.0
39	4210.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.85979325634555	18.93940223641123	21.024987913616386	23.175816593626838
2	35.097449890401144	30.83334246005235	22.87432194996785	11.19488569957866
3	23.52941176470588	29.120909208963795	36.940525605140586	10.409153421189739
4	23.567118152418292	20.22908586260794	43.42414679519173	12.779649189782038
5	26.246931466684607	24.473948953999773	34.17638140715232	15.1027381721633
6	16.90388313210222	39.70044543604074	34.21377487878413	9.181896553072914
7	64.93415463373955	4.296963618811889	28.15822288699505	2.6106588604535093
8	61.856937897110065	12.081376955921076	20.9282968447107	5.133388302258159
9	58.696641941081026	4.714706586414122	21.33743462009641	15.251216852408437
10	37.508468291431264	20.374278924006763	28.40464430046828	13.712608484093694
11	30.23457753982248	20.64307383724296	32.86980031260317	16.252548310331395
12	26.268992050366975	15.009958554265124	39.32197343655392	19.399075958813984
13	19.874551943287088	23.845613464153114	42.42578804003448	13.854046552525311
14	16.066832616494743	30.590519581506033	35.10449050221467	18.238157299784557
15	12.642435488438533	20.439521926812056	52.39607665818142	14.521965926567985
16	13.800224986661952	20.59910912791853	41.67353778226986	23.927128103149656
17	14.27319764249025	19.510317625467614	40.52779555314957	25.68868917889256
18	16.242534995752163	20.9616224072947	40.81520897318153	21.98063362377161
19	19.8440426254285	24.241921680234185	35.217766123392195	20.696269570945116
20	21.243403337875833	21.52080344332855	41.773045095900954	15.462748122894663
21	19.210231104171328	27.205080505484634	37.62956681462382	15.955121575720216
22	16.409006350631856	19.3363362846535	38.592878968753766	25.661778395960877
23	15.954182827478414	28.75464093662041	36.22958027001529	19.06159596588589
24	18.72990492044891	20.524009268574307	38.49399748728387	22.252088323692913
25	15.958876568687428	27.78632212520085	36.476314599569115	19.778486706542605
26	15.240734163708305	24.98149883673447	36.11552235863625	23.662244640920974
27	19.311146540165126	27.405659713149827	33.57839877712396	19.70479496956109
28	15.332731491404978	24.187787198290227	42.53796845492992	17.94151285537488
29	18.525414261776206	24.271805165931575	36.17935723907883	21.02342333321338
30	17.788966266081932	27.980173637133127	36.07093181715062	18.159928279634325
31	26.441878184898982	23.692910416819863	29.38328934254767	20.481922055733484
32	23.11307691946635	27.23261712057752	30.188109501853244	19.466196458102882
33	23.447271293548138	23.1634564084431	28.9879198747084	24.401352423300356
34	24.51102950955098	25.79132565332966	30.317030927060827	19.380613910058532
35	17.691336448934443	24.13177521986266	33.811208341091046	24.36567999011185
36	26.06888221682268	24.649651333257193	31.09587905167653	18.1855873982436
37	20.20952860757038	26.716931419747198	32.889826941761626	20.183713030920803
38	25.559298379564076	25.556638592878965	28.12614898873346	20.7579140388235
39	24.74180511899416	22.27148912069017	29.953891815523452	23.032813944792217
40	24.408080119033276	21.806182908836593	34.08485345357655	19.700883518553578
41	21.26186538663129	23.58526728509315	32.422643233424445	22.730224094851124
42	20.637441347792144	22.666545672448834	35.92433063338908	20.77168234636994
43	22.33641920741486	22.293080330251634	32.34191088462941	23.028589577704103
44	21.630011155458273	23.51251429635343	32.408249093716805	22.44922545447149
45	19.700883518553578	24.580340421404085	29.95889847281307	25.75987758722927
46	26.877144453014868	25.095869664194108	28.701601660958552	19.32538422183247
47	18.883546716023965	22.73554366822134	38.22254278736257	20.158366828392126
48	21.11510774482945	24.08170864696651	30.651694675263514	24.151488932940516
49	19.25529101977786	19.33962190349981	39.46184692458253	21.943240152139797
50	20.911868750479154	21.152970590582164	35.00357506622087	22.931585592717816
51	19.087255084495165	22.739142203148248	29.27173475981344	28.90186795254315
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	64.0
1	196.0
2	328.0
3	7711.5
4	15095.0
5	12467.5
6	9840.0
7	9814.0
8	9788.0
9	10008.0
10	10228.0
11	10144.0
12	10060.0
13	9412.0
14	8764.0
15	8275.5
16	7787.0
17	7308.5
18	6830.0
19	6373.5
20	5917.0
21	5427.0
22	4937.0
23	4872.5
24	4808.0
25	4725.0
26	4619.0
27	4596.0
28	5386.5
29	6177.0
30	6561.5
31	6946.0
32	7496.5
33	8047.0
34	9828.0
35	11609.0
36	12493.5
37	13378.0
38	14810.0
39	16242.0
40	19738.0
41	23234.0
42	28992.5
43	34751.0
44	40785.0
45	46819.0
46	77740.5
47	108662.0
48	84522.0
49	60382.0
50	55867.0
51	51352.0
52	42910.5
53	34469.0
54	28931.0
55	23393.0
56	22445.5
57	21498.0
58	20792.5
59	20087.0
60	18182.0
61	16277.0
62	14119.0
63	11961.0
64	9872.0
65	7783.0
66	6254.0
67	4725.0
68	3927.5
69	3130.0
70	2555.0
71	1980.0
72	1575.5
73	1171.0
74	958.0
75	558.0
76	371.0
77	258.5
78	146.0
79	105.0
80	64.0
81	51.5
82	39.0
83	26.0
84	13.0
85	10.0
86	7.0
87	5.0
88	3.0
89	2.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	639149.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.191454448966
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.89982418984042	27.847571928733437
2	7.645028113976626	5.074992048122392
3	2.513585368413426	2.50288662777845
4	1.173551006323258	1.5580745907966649
5	0.6895319678882956	1.1443284451635125
6	0.4105233449704683	0.8175520142894672
7	0.32077294013760704	0.7452844301726795
8	0.23843308363134913	0.6331152667581139
9	0.16889854615439656	0.5045389561062211
>10	1.8994465947698391	15.215195136885526
>50	0.7176199615781311	17.227692918911984
>100	0.31461919332908206	14.16265010518096
>500	0.002882007877823652	0.7327405342993222
>1k	0.003362342524127595	3.6625866611092968
>5k	4.8033464630394207E-4	0.8714446824369223
>10k+	0.001441003938911826	7.29934565325504
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGC	17723	2.772905848245088	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCG	14610	2.28585196878975	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTC	13451	2.1045171000815146	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5466	0.8551996482823254	No Hit
GCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	4694	0.7344140411703688	TruSeq Adapter, Index 21 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCT	4536	0.7096936708028957	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	3408	0.5332090013439745	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTC	3317	0.5189713196766325	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCT	2916	0.4562316455161472	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTA	2066	0.32324231126075453	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGCGGTTT	2036	0.3185485700517407	No Hit
CTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGCT	953	0.14910451240634032	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGT	853	0.1334587083762941	No Hit
CCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	850	0.13298933425539272	TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTAT	740	0.1157789498223419	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5645804030046202E-4	0.0	0.0	0.19432088605317382	0.0
2	1.5645804030046202E-4	0.0	0.0	1.3588380800095126	0.0
3	1.5645804030046202E-4	0.0	0.0	1.5864845286466849	0.0
4	1.5645804030046202E-4	0.0	0.0	2.7053159748352886	0.0
5	1.5645804030046202E-4	0.0	0.0	5.954167181674383	0.0
6	1.5645804030046202E-4	0.0	0.0	6.5812510071986345	0.0
7	1.5645804030046202E-4	0.0	0.0	7.508108437938572	0.0
8	1.5645804030046202E-4	0.0	0.0	9.021839977845541	0.0
9	1.5645804030046202E-4	0.0	0.0	9.439739403488076	0.0
10	1.5645804030046202E-4	0.0	0.0	12.713310980694642	0.0
11	1.5645804030046202E-4	0.0	0.0	13.572422079984479	0.0
12	1.5645804030046202E-4	0.0	0.0	17.530966957626468	0.0
13	1.5645804030046202E-4	0.0	0.0	18.393363675762615	0.0
14	1.5645804030046202E-4	0.0	0.0	18.68938228801109	0.0
15	1.5645804030046202E-4	0.0	0.0	19.299881561263494	0.0
16	1.5645804030046202E-4	0.0	0.0	19.8778375621334	0.0
17	1.5645804030046202E-4	0.0	0.0	20.31498132673289	0.0
18	3.1291608060092404E-4	0.0	0.0	21.172997219740623	0.0
19	3.1291608060092404E-4	0.0	0.0	21.813379978690413	0.0
20	3.1291608060092404E-4	0.0	0.0	22.229245449809042	0.0
21	3.1291608060092404E-4	0.0	0.0	22.507114929382663	0.0
22	3.1291608060092404E-4	0.0	0.0	22.79390251725341	0.0
23	3.1291608060092404E-4	0.0	0.0	23.057221399079086	0.0
24	3.1291608060092404E-4	0.0	0.0	23.269847875847415	0.0
25	3.1291608060092404E-4	0.0	0.0	23.452121492797453	0.0
26	3.1291608060092404E-4	0.0	0.0	23.634864483868395	0.0
27	3.1291608060092404E-4	0.0	0.0	23.844831173951615	0.0
28	3.1291608060092404E-4	0.0	0.0	24.0094250323477	0.0
29	3.1291608060092404E-4	0.0	0.0	24.19592301638585	0.0
30	3.1291608060092404E-4	0.0	0.0	24.41856280773341	0.0
31	3.1291608060092404E-4	0.0	0.0	24.603809127449153	0.0
32	3.1291608060092404E-4	0.0	0.0	24.773253185094557	0.0
33	3.1291608060092404E-4	0.0	0.0	24.972424270397042	0.0
34	3.1291608060092404E-4	0.0	0.0	25.162207873281503	0.0
35	3.1291608060092404E-4	0.0	0.0	25.38547349679026	0.0
36	3.1291608060092404E-4	0.0	0.0	25.559454837604378	0.0
37	3.1291608060092404E-4	0.0	0.0	25.751585311093343	0.0
38	3.1291608060092404E-4	0.0	0.0	25.93636225668819	0.0
39	3.1291608060092404E-4	0.0	0.0	26.14945810757742	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCAAG	70	0.0	45.000004	1
AACGTAG	25	3.8888542E-5	45.000004	1
CTAGACC	25	3.8888542E-5	45.000004	25
CGAACTT	25	3.8888542E-5	45.000004	12
CTCAAAT	25	3.8888542E-5	45.000004	40
GGGTCTA	25	3.8888542E-5	45.000004	7
TAGACGG	25	3.8888542E-5	45.000004	2
CGTGAAT	25	3.8888542E-5	45.000004	27
GTTAGCG	25	3.8888542E-5	45.000004	1
CACGATG	25	3.8888542E-5	45.000004	42
TAGCACG	50	2.1827873E-11	45.000004	1
AGAGTAG	25	3.8888542E-5	45.000004	1
ACCTATG	25	3.8888542E-5	45.000004	34
ACTAGCC	25	3.8888542E-5	45.000004	22
CATAGTT	25	3.8888542E-5	45.000004	18
GTTCGGT	25	3.8888542E-5	45.000004	29
ACGTGTG	25	3.8888542E-5	45.000004	37
AGCGCTA	35	1.2107921E-7	45.000004	19
CGAGCTT	25	3.8888542E-5	45.000004	17
TACTCAC	25	3.8888542E-5	45.000004	30
>>END_MODULE