FastQCFastQC Report
Sat 18 Jun 2016
SRR3548269_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548269_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476139
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGC93621.9662325497386268No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTC88441.8574407893493288No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCG84081.7658708906432785No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74561.5659292769548387No Hit
GCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC31890.6697624013155822TruSeq Adapter, Index 21 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCT27880.5855432972304306No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTC23130.48578251308966497No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGCGGTTT21650.45469915297843694No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCT20920.43936749562627725No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC18830.39547275060434034No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTA15230.3198645773608127No Hit
CTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGCT14510.3047429427121072TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC12710.26693885609034335TruSeq Adapter, Index 21 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC8600.18061952497064934TruSeq Adapter, Index 21 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC6970.14638582430760766TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGC6940.14575575619724493No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTT6350.13336441669344457No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGT5950.12496350855527483No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5170.10858173768584384No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCG4980.10459130632021323No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATCG253.8873506E-545.00000427
TTGTGCG351.209919E-745.0000041
CACGGGT253.8873506E-545.0000044
GCGAACC351.209919E-745.00000433
GCGCGGA253.8873506E-545.00000430
CGCGCAT502.1827873E-1145.00000436
GTAGGTC253.8873506E-545.00000442
CACGACA253.8873506E-545.00000414
TGCGGAA253.8873506E-545.00000424
CCCACTA253.8873506E-545.00000434
CAGGGTA502.1827873E-1145.0000045
GACTTAC253.8873506E-545.00000414
TACGCGC502.1827873E-1145.00000434
CACTCAA351.209919E-745.00000414
CCATATT253.8873506E-545.00000412
GTCAATG253.8873506E-545.0000041
CTATTTC253.8873506E-545.00000425
CTATTCC253.8873506E-545.00000411
CAATGCA253.8873506E-545.00000422
CGAGAAA253.8873506E-545.00000417
Produced by FastQC (version 0.11.5)