##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548269_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	476139
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.4747248177528	30.0	25.0	31.0	16.0	33.0
2	27.815873095881663	31.0	25.0	33.0	16.0	33.0
3	27.732403772847846	30.0	25.0	33.0	16.0	34.0
4	31.983143577820762	35.0	32.0	35.0	19.0	37.0
5	33.126421066117246	35.0	32.0	35.0	28.0	37.0
6	31.910440018566007	35.0	32.0	35.0	25.0	37.0
7	33.67363732019431	35.0	33.0	35.0	30.0	37.0
8	33.35942235355642	35.0	33.0	37.0	28.0	37.0
9	36.01431304724041	38.0	35.0	39.0	31.0	39.0
10	35.35079042044445	37.0	34.0	39.0	31.0	39.0
11	35.74911107890763	37.0	35.0	39.0	31.0	39.0
12	35.73010192401799	37.0	35.0	39.0	31.0	39.0
13	35.576491318711554	37.0	35.0	39.0	30.0	39.0
14	36.26401534005826	38.0	35.0	40.0	30.0	41.0
15	36.696924637553316	38.0	35.0	40.0	31.0	41.0
16	36.41910450519701	38.0	34.0	40.0	32.0	41.0
17	35.933710534108734	37.0	34.0	40.0	30.0	41.0
18	35.96778251729012	38.0	34.0	39.0	30.0	40.0
19	35.52930971838056	37.0	34.0	39.0	29.0	40.0
20	35.473426877445455	37.0	34.0	39.0	30.0	40.0
21	35.81035159900785	37.0	34.0	39.0	30.0	40.0
22	36.21861053179849	38.0	35.0	40.0	31.0	41.0
23	36.395892796011246	38.0	35.0	40.0	31.0	41.0
24	36.37214972938575	38.0	35.0	40.0	31.0	41.0
25	35.68175889813689	37.0	34.0	40.0	30.0	41.0
26	35.848565229901354	37.0	34.0	40.0	30.0	41.0
27	36.00815098112106	38.0	35.0	40.0	30.0	41.0
28	35.84983586725725	38.0	35.0	40.0	30.0	41.0
29	35.815461451382895	38.0	35.0	40.0	30.0	41.0
30	35.2588193783748	37.0	34.0	40.0	27.0	41.0
31	35.38357286422662	37.0	34.0	40.0	29.0	41.0
32	34.95086098807281	37.0	34.0	40.0	25.0	41.0
33	34.849396919807035	38.0	34.0	40.0	25.0	41.0
34	34.530996200689295	38.0	34.0	40.0	23.0	41.0
35	34.2029302367586	38.0	34.0	40.0	21.0	41.0
36	33.9836140286765	37.0	33.0	40.0	19.0	41.0
37	34.11106420604067	38.0	34.0	40.0	20.0	41.0
38	33.99104883237878	37.0	33.0	40.0	20.0	41.0
39	33.957098662365404	37.0	33.0	40.0	18.0	41.0
40	33.71798151380164	37.0	33.0	40.0	18.0	41.0
41	33.40046919071952	37.0	33.0	40.0	18.0	41.0
42	33.424907432493455	37.0	33.0	40.0	17.0	41.0
43	33.479250807012235	37.0	33.0	40.0	17.0	41.0
44	33.71198326539099	37.0	33.0	40.0	18.0	41.0
45	33.76545714591747	37.0	33.0	40.0	18.0	41.0
46	33.527755550375	37.0	33.0	40.0	18.0	41.0
47	33.4216268778655	37.0	33.0	40.0	18.0	41.0
48	33.50058281300208	37.0	33.0	40.0	18.0	41.0
49	33.548648608914625	37.0	33.0	40.0	18.0	41.0
50	33.41971357103703	36.0	33.0	40.0	18.0	41.0
51	31.44077464773942	35.0	29.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	16.0
10	21.0
11	18.0
12	12.0
13	20.0
14	28.0
15	61.0
16	125.0
17	252.0
18	608.0
19	1167.0
20	2016.0
21	2994.0
22	3901.0
23	4713.0
24	5966.0
25	8172.0
26	10193.0
27	10927.0
28	10522.0
29	10754.0
30	13058.0
31	17318.0
32	24969.0
33	37092.0
34	47986.0
35	53204.0
36	66943.0
37	76309.0
38	55853.0
39	10917.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.244084185500455	21.687994472202448	22.19960977781698	17.868311564480123
2	33.28019758935941	31.539319400427186	23.04432109110995	12.136161919103456
3	24.680397951018502	30.749424012735776	32.96411342066077	11.606064615584945
4	24.071332111001198	22.067715520047717	40.379385011519744	13.481567357431338
5	26.767183532539867	25.910080879743102	31.041775615944083	16.280959971772948
6	18.11571830914922	39.7783000342337	30.80781032429606	11.298171332321024
7	66.50851957096562	5.412915136126215	24.21078718609482	3.8677781068133466
8	62.236447760002854	11.605434547474582	18.042840431050596	8.115277261471965
9	58.20338178557102	6.209741273031614	19.857856634302166	15.729020307095196
10	36.89006781633095	21.594744391868762	25.771465895463297	15.743721896336993
11	28.66536872635932	21.83143997866169	31.065508181434414	18.437683113544573
12	24.61823123079605	15.755693190433886	37.28113009016275	22.34494548860732
13	21.955563396403154	22.99538580120511	40.48124602269505	14.567804779696687
14	15.947233895984155	31.805418165703713	32.68541329317699	19.56193464513514
15	12.301659809425399	21.931410785505914	48.53477660935147	17.23215279571722
16	13.219669046223897	23.52401294579944	38.001928008417714	25.254389999558953
17	13.813613251592496	23.022898775357618	36.399454781061834	26.76403319198805
18	15.801478139786912	22.43399511487192	37.95026242336797	23.814264321973205
19	18.79031123264425	26.675823656537272	32.60791491560238	21.925950195216103
20	20.970346894499293	24.064191339083756	37.52391633535585	17.441545431061098
21	18.338762420217623	29.89484163238046	34.6829392257303	17.083456721671613
22	15.538319692358742	21.413914844194657	35.46359361446972	27.584171848976872
23	15.939673078659803	29.761057170280107	33.29405908778739	21.0052106632727
24	18.610321775783962	22.88302365485709	34.24588197984202	24.260772589516925
25	16.200521276349974	29.768407964901005	31.76824414719231	22.26282661155671
26	14.610439388497898	27.000098710670624	34.09550572416878	24.293956176662697
27	20.203133958780946	28.42783304875257	30.257130795839032	21.111902196627454
28	15.468382132108479	26.116533197238624	37.89082599829042	20.524258672362482
29	18.296547856823324	26.252417886373518	32.46446940914313	22.98656484766003
30	19.438651318207498	29.97255003265853	30.999351029846327	19.589447619287643
31	25.51376803832494	26.118423401569707	25.49087556364843	22.876932996456915
32	23.49376967650203	28.684480792373655	26.06297740785779	21.758772123266525
33	24.27316392902073	24.618441253499505	25.8458139324861	25.262580884993667
34	25.608488277582808	25.406866482266732	28.18294657652492	20.801698663625537
35	18.68445979010331	25.02399509386965	31.12284437947742	25.16870073654962
36	25.730931513696632	25.526159377828744	30.387554894684115	18.355354213790513
37	22.404171890981413	25.144338102948925	31.778115214254658	20.673374791814993
38	26.448999136806687	23.61201245854677	27.732237854912118	22.206750549734426
39	26.491003677497538	20.420087411449177	29.143800444828088	23.9451084662252
40	25.96531685075157	20.678415336697896	33.70192317789553	19.654344634655004
41	21.970685031051858	22.504562743232544	31.999899189102344	23.52485303661326
42	21.936871375795725	21.745540692948907	35.6927283839383	20.624859547317065
43	22.86937217913256	21.500444198017806	32.121502334402344	23.50868128844728
44	23.039910614337412	22.435465273796098	30.49970701832868	24.024917093537812
45	21.328015558481873	21.46999090601694	28.95099960305709	28.250993932444096
46	28.11216892546084	23.00924729963309	27.951291534614892	20.927292240291177
47	19.39517661859247	21.57584234855788	37.06291650127379	21.966064531575864
48	21.126393763165797	21.822619025116616	31.19047168998969	25.8605155217279
49	20.57718439363295	16.99566723162774	38.67547081839547	23.751677556343843
50	20.716849491430022	18.35829453163887	35.59611794034935	25.328738036581754
51	19.201955731414564	19.843365067763823	29.126368560441385	31.828310640380224
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	616.0
1	618.0
2	620.0
3	6171.0
4	11722.0
5	7847.0
6	3972.0
7	4002.5
8	4033.0
9	4176.5
10	4320.0
11	4381.5
12	4443.0
13	4235.0
14	4027.0
15	3849.5
16	3672.0
17	3511.5
18	3351.0
19	3286.0
20	3221.0
21	3038.5
22	2856.0
23	2866.5
24	2877.0
25	2981.0
26	3308.5
27	3532.0
28	4199.5
29	4867.0
30	5226.5
31	5586.0
32	5926.0
33	6266.0
34	6977.5
35	7689.0
36	8381.0
37	9073.0
38	9966.5
39	10860.0
40	13080.0
41	15300.0
42	19381.5
43	23463.0
44	28457.5
45	33452.0
46	55819.0
47	78186.0
48	68965.5
49	59745.0
50	54532.0
51	49319.0
52	40699.5
53	32080.0
54	26342.5
55	20605.0
56	18730.0
57	16855.0
58	15427.0
59	13999.0
60	12697.0
61	11395.0
62	9666.0
63	7937.0
64	6574.0
65	5211.0
66	4214.5
67	3218.0
68	2577.5
69	1937.0
70	1584.0
71	1231.0
72	975.5
73	720.0
74	578.0
75	326.0
76	216.0
77	150.5
78	85.0
79	57.0
80	29.0
81	27.0
82	25.0
83	15.5
84	6.0
85	3.0
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	476139.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.701953090536456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.54875955614587	25.359958972343726
2	9.5261147822435	6.230451174879856
3	3.759243691723623	3.6880383258792295
4	1.9515196463119402	2.5527401571581345
5	1.140318130689784	1.8645315009052768
6	0.7620486891526808	1.4952288291225457
7	0.5469791206560803	1.252109988163861
8	0.39633034121380534	1.0368620981384136
9	0.32995901815372247	0.971127390011625
>10	3.291645869310913	25.13159892737805
>50	0.6641441311865344	14.56749466204808
>100	0.07062605882861683	3.8265365251189274
>500	0.003887672963043128	0.8471395441289923
>1k	0.005831509444564693	3.957061277290878
>5k	0.0025917819753620854	7.2191206274324085
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGC	9362	1.9662325497386268	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTC	8844	1.8574407893493288	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCG	8408	1.7658708906432785	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7456	1.5659292769548387	No Hit
GCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	3189	0.6697624013155822	TruSeq Adapter, Index 21 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCT	2788	0.5855432972304306	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTC	2313	0.48578251308966497	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGCGGTTT	2165	0.45469915297843694	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCT	2092	0.43936749562627725	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1883	0.39547275060434034	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTA	1523	0.3198645773608127	No Hit
CTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGCT	1451	0.3047429427121072	TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	1271	0.26693885609034335	TruSeq Adapter, Index 21 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	860	0.18061952497064934	TruSeq Adapter, Index 21 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	697	0.14638582430760766	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGC	694	0.14575575619724493	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTT	635	0.13336441669344457	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGT	595	0.12496350855527483	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	517	0.10858173768584384	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCG	498	0.10459130632021323	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.100227034542434E-4	0.0	0.0	0.34632743799604737	0.0
2	2.100227034542434E-4	0.0	0.0	1.7513793241049358	0.0
3	2.100227034542434E-4	0.0	0.0	2.2888274222443448	0.0
4	2.100227034542434E-4	0.0	0.0	3.7581462556102316	0.0
5	2.100227034542434E-4	0.0	0.0	7.676959879362959	0.0
6	2.100227034542434E-4	0.0	0.0	9.248769792014517	0.0
7	2.100227034542434E-4	0.0	0.0	10.6956161961108	0.0
8	2.100227034542434E-4	0.0	0.0	13.400498593898	0.0
9	2.100227034542434E-4	0.0	0.0	14.283224016516185	0.0
10	2.100227034542434E-4	0.0	0.0	18.08862538040362	0.0
11	2.100227034542434E-4	0.0	0.0	20.186332142504604	0.0
12	2.100227034542434E-4	0.0	0.0	24.38510602996184	0.0
13	2.100227034542434E-4	0.0	0.0	25.418207708253263	0.0
14	2.100227034542434E-4	0.0	0.0	25.779236735491107	0.0
15	2.100227034542434E-4	0.0	0.0	26.580893394575952	0.0
16	2.100227034542434E-4	0.0	0.0	27.600763642549758	0.0
17	2.100227034542434E-4	0.0	0.0	28.5135223117619	0.0
18	2.100227034542434E-4	0.0	0.0	30.182152690705866	0.0
19	2.100227034542434E-4	0.0	0.0	31.338327673221475	0.0
20	2.100227034542434E-4	0.0	0.0	32.14691508152031	0.0
21	2.100227034542434E-4	0.0	0.0	32.73917910526128	0.0
22	2.100227034542434E-4	0.0	0.0	33.3219921073468	0.0
23	2.100227034542434E-4	0.0	0.0	33.87813222609364	0.0
24	2.100227034542434E-4	0.0	0.0	34.33850199206534	0.0
25	2.100227034542434E-4	0.0	0.0	34.72158340316588	0.0
26	2.100227034542434E-4	0.0	0.0	35.070431113603384	0.0
27	2.100227034542434E-4	0.0	0.0	35.44070113979321	0.0
28	2.100227034542434E-4	0.0	0.0	35.77274703395437	0.0
29	2.100227034542434E-4	0.0	0.0	36.1205446308746	0.0
30	2.100227034542434E-4	0.0	0.0	36.530718970720734	0.0
31	4.200454069084868E-4	0.0	0.0	36.88859765740676	0.0
32	4.200454069084868E-4	0.0	0.0	37.21245266613321	0.0
33	6.300681103627303E-4	0.0	0.0	37.58188260150922	0.0
34	6.300681103627303E-4	0.0	0.0	37.88557543070406	0.0
35	6.300681103627303E-4	0.0	0.0	38.259205820149155	0.0
36	6.300681103627303E-4	0.0	0.0	38.58600114672396	0.0
37	6.300681103627303E-4	0.0	0.0	38.914476654926396	0.0
38	6.300681103627303E-4	0.0	0.0	39.222789983597224	0.0
39	6.300681103627303E-4	0.0	0.0	39.6002007817045	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGATCG	25	3.8873506E-5	45.000004	27
TTGTGCG	35	1.209919E-7	45.000004	1
CACGGGT	25	3.8873506E-5	45.000004	4
GCGAACC	35	1.209919E-7	45.000004	33
GCGCGGA	25	3.8873506E-5	45.000004	30
CGCGCAT	50	2.1827873E-11	45.000004	36
GTAGGTC	25	3.8873506E-5	45.000004	42
CACGACA	25	3.8873506E-5	45.000004	14
TGCGGAA	25	3.8873506E-5	45.000004	24
CCCACTA	25	3.8873506E-5	45.000004	34
CAGGGTA	50	2.1827873E-11	45.000004	5
GACTTAC	25	3.8873506E-5	45.000004	14
TACGCGC	50	2.1827873E-11	45.000004	34
CACTCAA	35	1.209919E-7	45.000004	14
CCATATT	25	3.8873506E-5	45.000004	12
GTCAATG	25	3.8873506E-5	45.000004	1
CTATTTC	25	3.8873506E-5	45.000004	25
CTATTCC	25	3.8873506E-5	45.000004	11
CAATGCA	25	3.8873506E-5	45.000004	22
CGAGAAA	25	3.8873506E-5	45.000004	17
>>END_MODULE