SRR3548268_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548268_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1098314
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC	28683	2.611548245765783	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG	26671	2.4283583747452915	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC	22127	2.0146333380071635	TruSeq Adapter, Index 23 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	8321	0.7576157637979667	TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7787	0.7089957880897448	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCT	6697	0.6097527665130373	Illumina PCR Primer Index 5 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTC	5788	0.526989549436682	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCT	5187	0.47226931460402033	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCTGTTCT	4375	0.3983378159615556	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTA	3691	0.3360605437060804	No Hit
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT	2213	0.20149064839381087	Illumina PCR Primer Index 5 (95% over 24bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1610	0.14658831627385246	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGT	1603	0.14595097576831398	No Hit
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	1482	0.13493408988686295	TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTAT	1251	0.11390185320409281	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTT	1104	0.10051770258778456	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGTT	35	1.2118653E-7	45.000004	32
GAGTACG	30	2.1654123E-6	45.000004	1
CTACGAC	30	2.1654123E-6	45.000004	27
TGCCTAA	35	1.2118653E-7	45.000004	44
CGTAATC	35	1.2118653E-7	45.000004	33
CGTCGGA	30	2.1654123E-6	45.000004	27
CGACTTA	30	2.1654123E-6	45.000004	13
AGTACGC	30	2.1654123E-6	45.000004	17
GCGTAAG	35	1.2118653E-7	45.000004	1
GTCGGAT	30	2.1654123E-6	45.000004	28
TCACGAC	40	6.8139343E-9	45.0	25
AACGTCC	20	7.0331496E-4	45.0	14
CCTTACG	20	7.0331496E-4	45.0	25
GCCCATA	20	7.0331496E-4	45.0	41
AGTCCGT	20	7.0331496E-4	45.0	35
TCGTTAA	25	3.890693E-5	45.0	21
CGTATTA	20	7.0331496E-4	45.0	17
GTATTCG	20	7.0331496E-4	45.0	43
GTCGACG	40	6.8139343E-9	45.0	33
ATCTAAC	20	7.0331496E-4	45.0	32