##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548268_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1098314
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.65172072831631	28.0	25.0	31.0	16.0	33.0
2	27.535269513090064	30.0	25.0	31.0	16.0	33.0
3	27.473179801040505	30.0	25.0	31.0	16.0	33.0
4	30.48588381828876	33.0	28.0	35.0	19.0	37.0
5	31.82058045331299	33.0	32.0	35.0	26.0	37.0
6	31.06954386450505	35.0	30.0	35.0	17.0	37.0
7	31.835355827204243	33.0	30.0	35.0	26.0	35.0
8	33.06962398731146	35.0	32.0	35.0	28.0	37.0
9	34.2394561118223	37.0	33.0	37.0	27.0	39.0
10	33.69842868250792	35.0	32.0	38.0	27.0	39.0
11	33.828838565291896	37.0	32.0	39.0	26.0	39.0
12	33.234855423858754	35.0	31.0	37.0	25.0	39.0
13	33.40198795608542	35.0	32.0	38.0	24.0	39.0
14	34.11906522178539	36.0	32.0	39.0	24.0	40.0
15	35.066081284587106	37.0	33.0	39.0	27.0	41.0
16	35.109872950722654	36.0	33.0	39.0	27.0	41.0
17	34.660580671829734	36.0	33.0	39.0	27.0	40.0
18	34.499566608456234	36.0	33.0	39.0	27.0	40.0
19	33.81618553528408	36.0	32.0	38.0	25.0	40.0
20	33.70714567965081	35.0	32.0	38.0	25.0	40.0
21	34.25697751280599	35.0	33.0	39.0	27.0	40.0
22	34.55900407351632	35.0	33.0	39.0	27.0	40.0
23	34.92452067441551	35.0	33.0	39.0	29.0	40.0
24	34.68069149623878	35.0	33.0	39.0	27.0	40.0
25	33.99013852140644	35.0	33.0	39.0	25.0	40.0
26	33.85625422238085	35.0	32.0	39.0	24.0	40.0
27	34.321134939552806	35.0	33.0	39.0	25.0	40.0
28	34.206039438630484	35.0	33.0	39.0	25.0	40.0
29	34.341920434411286	36.0	33.0	39.0	25.0	40.0
30	33.79676668056676	35.0	33.0	39.0	23.0	40.0
31	33.808104968160286	35.0	33.0	39.0	23.0	40.0
32	33.28005834397085	35.0	32.0	39.0	21.0	40.0
33	33.198051741123216	36.0	32.0	39.0	18.0	40.0
34	32.94984221270056	36.0	32.0	39.0	17.0	40.0
35	32.92292368120592	36.0	32.0	40.0	15.0	40.0
36	32.6254468212187	36.0	32.0	40.0	14.0	40.0
37	32.62878921692703	36.0	32.0	40.0	12.0	40.0
38	32.58938973736108	36.0	32.0	40.0	12.0	40.0
39	32.38127074770967	35.0	31.0	39.0	11.0	40.0
40	32.23104139617632	35.0	31.0	39.0	10.0	40.0
41	31.911741997279467	35.0	30.0	39.0	10.0	40.0
42	32.152946243059816	35.0	31.0	39.0	10.0	40.0
43	32.063069395455216	35.0	31.0	39.0	10.0	40.0
44	31.995208109884786	35.0	31.0	39.0	10.0	40.0
45	32.03800916677744	35.0	31.0	39.0	10.0	40.0
46	31.790131965904102	35.0	31.0	39.0	10.0	40.0
47	31.90376886755518	35.0	31.0	39.0	10.0	40.0
48	31.765523338498827	35.0	31.0	39.0	10.0	40.0
49	31.85841844864037	35.0	31.0	39.0	10.0	40.0
50	31.678725756022413	35.0	31.0	39.0	10.0	40.0
51	29.516201195650787	34.0	26.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	36.0
9	62.0
10	94.0
11	63.0
12	56.0
13	78.0
14	156.0
15	348.0
16	798.0
17	1718.0
18	3315.0
19	5501.0
20	8103.0
21	11468.0
22	15359.0
23	20170.0
24	25653.0
25	30980.0
26	33015.0
27	33302.0
28	34760.0
29	39627.0
30	48868.0
31	63111.0
32	81201.0
33	101299.0
34	112270.0
35	108458.0
36	121099.0
37	119581.0
38	70989.0
39	6776.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.926871550394516	18.87429277966046	22.151224513208426	22.0476111567366
2	33.5005289926196	30.240896501364816	24.803471502685024	11.45510300333056
3	23.396223666456038	29.83591213441693	35.372580154673436	11.39528404445359
4	21.659106594289064	22.759156306848496	42.42666486997343	13.155072228889006
5	26.342284628985883	25.231126981901355	34.02587966647061	14.400708722642158
6	17.273475527035075	39.529952272301	32.96789442727672	10.22867777338721
7	66.97428968400658	4.424690935379136	25.803640853162214	2.7973785274520764
8	64.70399175463483	11.24468958785921	18.86154596954969	5.189772687956268
9	60.8051977849686	4.832770956211065	20.114739500725655	14.24729175809468
10	41.232835054456196	19.08716450851032	25.604153274928663	14.075847162104827
11	35.42393158969111	19.68954233488784	31.279670476748905	13.606855598672146
12	28.97604874380186	17.338211112669054	34.95839987471707	18.727340268812014
13	19.524197998022423	28.338435092332432	39.80610280848646	12.331264101158686
14	15.959552550545656	33.49797963059744	33.74754396283758	16.794923856019317
15	11.677170645188898	19.7275096192892	54.32171491941284	14.273604816109053
16	13.463453984925986	19.466017914731122	43.26549602390573	23.805032076437158
17	13.83757286167708	20.064389600788118	38.95079185005381	27.147245687480993
18	17.326738983569363	20.78567695577039	40.56016767518214	21.327416385478106
19	19.856343450051625	24.238150474272384	34.406371948277084	21.499134127398904
20	21.660745469874733	22.128644449583636	38.41096444186271	17.79964563867892
21	19.174844352343683	28.558772809961454	35.55149073944246	16.714892098252413
22	16.73373916748762	21.496948959951343	36.57824629386496	25.191065578696076
23	15.753964713187669	29.449410642129664	34.65156594562211	20.145058699060563
24	18.10911997843968	20.689620636721376	38.92875807829091	22.272501306548037
25	13.967499276163284	28.018125963977514	36.95928486753333	21.055089892325874
26	14.363196681459037	24.760860737457595	37.6001762701741	23.275766310909265
27	19.363679239270372	26.446717423250544	33.69009226869547	20.499511068783608
28	12.90514370207427	23.698323066081283	43.98869540040462	19.407837831439824
29	17.4879861314706	21.026682715507587	40.12504620718666	21.36028494583516
30	19.86526621712916	25.430978754709493	35.98406284541579	18.719692182745554
31	23.799022865956367	21.59692037067724	31.532967803378632	23.07108895998776
32	25.314891734057838	24.168043018663152	33.904876019061945	16.612189228217066
33	23.934776393636064	20.88583046378358	30.15339875481875	25.025994387761607
34	20.10299422569502	21.75907800501496	38.14027682429615	19.99765094499387
35	20.966226416125078	21.250389232951598	35.14404805911606	22.63933629180726
36	24.729357906755265	19.72414081947421	32.46421333061401	23.08228794315651
37	20.95730364904754	23.573222229708442	37.26038273207844	18.209091389165575
38	23.003530866400684	25.080987768525215	27.73969921170084	24.175782153373262
39	20.15571139036742	22.942983518374525	36.42692344812139	20.474381643136663
40	23.6337695777346	21.252847546330102	33.934466828247665	21.178916047687636
41	17.163306668220564	22.382305970787954	32.22466434917519	28.229723011816294
42	20.215530349244386	21.291907414455245	36.07838924023549	22.414172996064877
43	22.4100758071007	21.18319533393911	29.759886516970557	26.64684234198963
44	21.292089511742542	23.766973743392146	32.07998805441795	22.86094869044736
45	17.876763839849076	25.253889142813442	30.879420639270737	25.989926378066748
46	25.748920618329546	25.39282937302083	30.362810635209968	18.495439373439655
47	18.97726879562675	21.881356333434702	38.13499600296454	21.006378867974004
48	21.68751377110735	23.057613760727808	31.570570893205403	23.684301574959438
49	19.509721263682334	19.15954818021076	38.14464715919127	23.186083396915635
50	20.778210966991224	22.716454492977416	32.65723645514853	23.848098084882828
51	18.938937316650794	22.460607804325537	29.523706335346724	29.076748543676946
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	88.0
1	372.0
2	656.0
3	10415.0
4	20174.0
5	16847.5
6	13521.0
7	13703.0
8	13885.0
9	14329.0
10	14773.0
11	14963.0
12	15153.0
13	14737.5
14	14322.0
15	13602.0
16	12882.0
17	12628.5
18	12375.0
19	11485.5
20	10596.0
21	9854.5
22	9113.0
23	8217.0
24	7321.0
25	8020.5
26	9226.5
27	9733.0
28	11458.5
29	13184.0
30	12454.5
31	11725.0
32	13671.5
33	15618.0
34	17043.5
35	18469.0
36	20217.0
37	21965.0
38	24582.5
39	27200.0
40	38132.5
41	49065.0
42	58699.0
43	68333.0
44	78499.5
45	88666.0
46	139700.0
47	190734.0
48	144949.5
49	99165.0
50	92908.0
51	86651.0
52	74479.5
53	62308.0
54	53541.5
55	44775.0
56	40027.0
57	35279.0
58	31907.5
59	28536.0
60	26527.0
61	24518.0
62	21503.5
63	18489.0
64	14292.0
65	10095.0
66	8377.5
67	6660.0
68	5613.0
69	4566.0
70	3994.0
71	3422.0
72	2930.0
73	2438.0
74	1877.5
75	1153.5
76	990.0
77	650.0
78	310.0
79	340.0
80	370.0
81	233.5
82	97.0
83	60.0
84	23.0
85	21.0
86	19.0
87	13.5
88	8.0
89	6.0
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1098314.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.71213207066666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.36621953493086	24.769881250540564
2	8.015363452444085	4.763070749868266
3	2.6425502215018213	2.355474035538946
4	1.2317358163692136	1.4638998900852993
5	0.7373716307590679	1.0954441639138137
6	0.503548769524858	0.897690452648656
7	0.33592935067690677	0.6986824063607883
8	0.23052195234928252	0.5479438954711847
9	0.19260192775872276	0.5150352523168952
>10	1.5637393423487744	10.391232062557208
>50	0.5738840816652211	12.430113398091915
>100	0.5986853958658767	27.470439797982248
>500	0.002511527617685881	0.5071554782244717
>1k	0.0028254685698966167	1.7156723764912098
>5k	0.001569704761053676	3.150949430646065
>10k+	9.418228566322055E-4	7.227315359262514
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC	28683	2.611548245765783	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG	26671	2.4283583747452915	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC	22127	2.0146333380071635	TruSeq Adapter, Index 23 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	8321	0.7576157637979667	TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7787	0.7089957880897448	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCT	6697	0.6097527665130373	Illumina PCR Primer Index 5 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTC	5788	0.526989549436682	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCT	5187	0.47226931460402033	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCTGTTCT	4375	0.3983378159615556	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTA	3691	0.3360605437060804	No Hit
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT	2213	0.20149064839381087	Illumina PCR Primer Index 5 (95% over 24bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1610	0.14658831627385246	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGT	1603	0.14595097576831398	No Hit
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	1482	0.13493408988686295	TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTAT	1251	0.11390185320409281	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTT	1104	0.10051770258778456	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8209728729671115E-4	0.0	0.0	0.22543644167332838	0.0
2	1.8209728729671115E-4	0.0	0.0	1.3733777407917955	0.0
3	1.8209728729671115E-4	0.0	0.0	1.5913481936859586	0.0
4	1.8209728729671115E-4	0.0	0.0	2.49764639256169	0.0
5	1.8209728729671115E-4	0.0	0.0	5.368410126794341	0.0
6	1.8209728729671115E-4	0.0	0.0	6.015857031777798	0.0
7	1.8209728729671115E-4	0.0	0.0	6.966860114684872	0.0
8	1.8209728729671115E-4	0.0	0.0	8.761519929637608	0.0
9	1.8209728729671115E-4	0.0	0.0	9.41688806661847	0.0
10	1.8209728729671115E-4	0.0	0.0	13.01913660392201	0.0
11	1.8209728729671115E-4	0.0	0.0	13.930988770060292	0.0
12	1.8209728729671115E-4	0.0	0.0	17.62801894540177	0.0
13	1.8209728729671115E-4	0.0	0.0	18.418958512775035	0.0
14	1.8209728729671115E-4	0.0	0.0	18.733440527936455	0.0
15	1.8209728729671115E-4	0.0	0.0	19.326531392661842	0.0
16	1.8209728729671115E-4	0.0	0.0	19.92290000855857	0.0
17	1.8209728729671115E-4	0.0	0.0	20.386519702016	0.0
18	3.641945745934223E-4	0.0	0.0	21.22753602339586	0.0
19	3.641945745934223E-4	0.0	0.0	21.938808027576812	0.0
20	3.641945745934223E-4	0.0	0.0	22.344429735030236	0.0
21	3.641945745934223E-4	0.0	0.0	22.58561759205473	0.0
22	3.641945745934223E-4	0.0	0.0	22.851934874726172	0.0
23	3.641945745934223E-4	0.0	0.0	23.077007121824906	0.0
24	3.641945745934223E-4	0.0	0.0	23.250454787975023	0.0
25	3.641945745934223E-4	0.0	0.0	23.39467583951402	0.0
26	3.641945745934223E-4	0.0	0.0	23.54199254493706	0.0
27	3.641945745934223E-4	0.0	0.0	23.736745593700892	0.0
28	3.641945745934223E-4	0.0	0.0	23.88406229912393	0.0
29	3.641945745934223E-4	0.0	0.0	24.02354882119321	0.0
30	3.641945745934223E-4	0.0	0.0	24.242065565949265	0.0
31	3.641945745934223E-4	0.0	0.0	24.39957971946092	0.0
32	3.641945745934223E-4	0.0	0.0	24.54889949504422	0.0
33	3.641945745934223E-4	0.0	0.0	24.6913906223539	0.0
34	3.641945745934223E-4	0.0	0.0	24.85637076464472	0.0
35	3.641945745934223E-4	0.0	0.0	25.00915038868666	0.0
36	3.641945745934223E-4	0.0	0.0	25.14362923535528	0.0
37	6.37340505538489E-4	0.0	0.0	25.26936741223366	0.0
38	6.37340505538489E-4	0.0	0.0	25.412131685474282	0.0
39	6.37340505538489E-4	0.0	0.0	25.555988542438683	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGTT	35	1.2118653E-7	45.000004	32
GAGTACG	30	2.1654123E-6	45.000004	1
CTACGAC	30	2.1654123E-6	45.000004	27
TGCCTAA	35	1.2118653E-7	45.000004	44
CGTAATC	35	1.2118653E-7	45.000004	33
CGTCGGA	30	2.1654123E-6	45.000004	27
CGACTTA	30	2.1654123E-6	45.000004	13
AGTACGC	30	2.1654123E-6	45.000004	17
GCGTAAG	35	1.2118653E-7	45.000004	1
GTCGGAT	30	2.1654123E-6	45.000004	28
TCACGAC	40	6.8139343E-9	45.0	25
AACGTCC	20	7.0331496E-4	45.0	14
CCTTACG	20	7.0331496E-4	45.0	25
GCCCATA	20	7.0331496E-4	45.0	41
AGTCCGT	20	7.0331496E-4	45.0	35
TCGTTAA	25	3.890693E-5	45.0	21
CGTATTA	20	7.0331496E-4	45.0	17
GTATTCG	20	7.0331496E-4	45.0	43
GTCGACG	40	6.8139343E-9	45.0	33
ATCTAAC	20	7.0331496E-4	45.0	32
>>END_MODULE