FastQCFastQC Report
Sat 18 Jun 2016
SRR3548267_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548267_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696634
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG116171.6675901549450645No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC108231.5536135187200164No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC96041.3786292371604028TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78131.1215358423505026No Hit
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC52930.7597963923667234TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCTGTTCT34400.493803058708016No Hit
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT30740.44126471001989565Illumina PCR Primer Index 5 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCT30390.4362405509923432Illumina PCR Primer Index 5 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCT27390.39317633075617897No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTC26990.38743443472469047No Hit
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC23460.3367622022468039TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTA18450.26484495445240974No Hit
TCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC13580.19493737026903654TruSeq Adapter, Index 23 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC11960.17168269134150788Illumina PCR Primer Index 5 (95% over 23bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC9880.14182483197776738No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9190.1319200613234496No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTT8190.11756532124472822No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC7830.11239761481638853No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGT7760.11139278301087802No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG7410.10636862398332554No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG253.889217E-545.0000041
ACCGCAA253.889217E-545.00000438
TTTCGAT253.889217E-545.00000413
GACCGAC253.889217E-545.0000049
AAGCGAC253.889217E-545.00000415
ACTAGTC302.1642645E-645.00000422
CGTAGGC253.889217E-545.00000432
CGTGTAT253.889217E-545.00000430
CGTCGGA253.889217E-545.00000427
TGTAACG351.2110104E-745.0000041
GCGTAAG302.1642645E-645.0000041
GTCGGAT253.889217E-545.00000428
CGAACTC406.8084773E-945.013
ACTGCAA207.031371E-445.019
CCTAGTC406.8084773E-945.030
TGGACCG207.031371E-445.045
TTTAACG207.031371E-445.01
CCGGAGC207.031371E-445.016
ACCGACA207.031371E-445.038
ATTCGCG207.031371E-445.031