SRR3548265_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548265_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	476597
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGC	12690	2.662626915402321	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCG	11123	2.3338376028384564	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTC	10268	2.1544407539283714	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4775	1.0018946825095416	No Hit
GCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	4160	0.872854843819831	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAAACCCAT	3285	0.689261577391381	No Hit
GAACTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCT	3113	0.6531723867334457	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTC	2839	0.595681466731851	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCT	2703	0.5671458276069719	No Hit
CTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGCT	2670	0.5602217387016704	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAAACCCATCGTA	2004	0.4204810353401301	No Hit
CCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	1543	0.32375361154182675	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGC	1071	0.22471815810842286	No Hit
TCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	1000	0.2098208759182286	No Hit
ACTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	924	0.19387448934844323	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	831	0.17436114788804796	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAAACCCATCGT	819	0.17184329737702922	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTT	815	0.17100401387335631	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCG	721	0.15128085153704282	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	630	0.13218715182848403	No Hit
GAACAAGGGAACACAACGAGCGACTGCCACAAAAAAAAGTGCACTCGGGAG	609	0.12778091343420123	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATG	563	0.11812915314196272	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTAT	537	0.11267381036808877	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.029124E-4	45.000004	25
GTTTGCG	20	7.029124E-4	45.000004	1
AGCGTGA	20	7.029124E-4	45.000004	22
CCTTACT	20	7.029124E-4	45.000004	45
ATCTCGC	20	7.029124E-4	45.000004	14
CTCACGA	20	7.029124E-4	45.000004	24
GTACCCT	20	7.029124E-4	45.000004	43
GGTACAC	20	7.029124E-4	45.000004	8
GTACAAA	20	7.029124E-4	45.000004	42
TACCAAC	20	7.029124E-4	45.000004	20
TTTACAG	40	6.8012014E-9	45.000004	1
CCTAGGC	20	7.029124E-4	45.000004	31
CCTAGCG	20	7.029124E-4	45.000004	20
CGTGCAA	20	7.029124E-4	45.000004	32
GCGAAGC	20	7.029124E-4	45.000004	40
GCGCGAG	20	7.029124E-4	45.000004	37
CCGGCTA	20	7.029124E-4	45.000004	21
AACTTGC	20	7.029124E-4	45.000004	28
AACTTCG	20	7.029124E-4	45.000004	16
CGCATGG	40	6.8012014E-9	45.000004	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content