##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548265_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	476597
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.519327649985208	30.0	25.0	31.0	16.0	33.0
2	27.722249615503245	30.0	25.0	31.0	16.0	33.0
3	27.673260637393856	30.0	25.0	33.0	16.0	34.0
4	32.01200385231128	35.0	32.0	35.0	19.0	37.0
5	33.16494019055932	35.0	32.0	35.0	28.0	37.0
6	31.855834174365345	35.0	32.0	35.0	25.0	37.0
7	33.6565211279131	35.0	33.0	35.0	30.0	37.0
8	33.08884025707254	35.0	33.0	36.0	27.0	37.0
9	35.827535632830255	37.0	35.0	39.0	31.0	39.0
10	35.252477459992406	37.0	34.0	39.0	30.0	39.0
11	35.55656456083442	37.0	35.0	39.0	30.0	39.0
12	35.492365667429716	37.0	34.0	39.0	30.0	39.0
13	35.25171790842158	37.0	34.0	39.0	30.0	39.0
14	35.89729268123803	38.0	34.0	40.0	29.0	41.0
15	36.42496071104098	38.0	35.0	40.0	31.0	41.0
16	36.221587630639725	37.0	34.0	40.0	31.0	41.0
17	35.717826591438886	37.0	34.0	39.0	30.0	41.0
18	35.84946820898998	37.0	34.0	39.0	30.0	40.0
19	35.34390900488253	37.0	34.0	39.0	29.0	40.0
20	35.197814925398184	36.0	34.0	39.0	29.0	40.0
21	35.57298094616626	37.0	34.0	39.0	30.0	40.0
22	36.05513463156503	37.0	34.0	40.0	31.0	40.0
23	36.1629468922381	37.0	35.0	40.0	31.0	41.0
24	36.151119289462585	38.0	35.0	40.0	31.0	41.0
25	35.46258159409312	37.0	34.0	40.0	29.0	41.0
26	35.648212221226736	37.0	34.0	40.0	30.0	41.0
27	35.692175989357885	37.0	34.0	40.0	30.0	41.0
28	35.48189980213891	37.0	34.0	40.0	29.0	41.0
29	35.58290757180595	37.0	34.0	40.0	30.0	41.0
30	34.967790397337794	37.0	34.0	40.0	27.0	41.0
31	34.91960922960069	37.0	34.0	40.0	27.0	41.0
32	34.52486692110945	37.0	33.0	40.0	25.0	41.0
33	34.25046108137483	37.0	33.0	40.0	23.0	41.0
34	34.07782046466931	37.0	33.0	40.0	21.0	41.0
35	33.92684804981987	37.0	33.0	40.0	20.0	41.0
36	33.67266474610625	37.0	33.0	40.0	18.0	41.0
37	33.522913488754654	37.0	33.0	40.0	17.0	41.0
38	33.47642557548621	37.0	33.0	40.0	18.0	41.0
39	33.37565909982648	37.0	33.0	40.0	17.0	41.0
40	33.42013902731238	37.0	33.0	40.0	18.0	41.0
41	33.0330530825834	36.0	32.0	40.0	15.0	40.0
42	33.30377236952813	37.0	33.0	40.0	16.0	41.0
43	33.1269227460517	37.0	33.0	40.0	15.0	41.0
44	33.22993640329251	37.0	33.0	40.0	15.0	41.0
45	33.3370688443276	37.0	33.0	40.0	17.0	41.0
46	33.0326313426228	36.0	32.0	40.0	15.0	40.0
47	32.810158267886706	36.0	32.0	39.0	15.0	40.0
48	32.98053701554983	36.0	32.0	40.0	15.0	40.0
49	33.10669391540442	36.0	32.0	40.0	15.0	40.0
50	32.899069864057054	36.0	32.0	39.0	15.0	40.0
51	30.863962635098417	34.0	28.0	38.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	11.0
11	13.0
12	14.0
13	11.0
14	30.0
15	58.0
16	123.0
17	298.0
18	703.0
19	1452.0
20	2542.0
21	3851.0
22	4957.0
23	5687.0
24	6695.0
25	8468.0
26	10160.0
27	10392.0
28	10142.0
29	11205.0
30	13988.0
31	19015.0
32	27154.0
33	40796.0
34	52237.0
35	54895.0
36	67832.0
37	70271.0
38	45240.0
39	8351.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.72307840796312	21.353680363074044	22.016504510099725	16.906736718863108
2	28.648312935247176	35.21381796360447	22.799975660778394	13.337893440369955
3	22.486922913908398	34.14897701831946	31.55013564919628	11.813964418575862
4	20.953761773573902	23.449790913497147	41.91360835254943	13.682838960379524
5	27.813016028216715	27.04654036848742	29.74714486243094	15.393298740864925
6	16.87925018411782	42.2575047681794	29.351632511325082	11.511612536377694
7	64.04635362790786	5.8884130617691675	25.730963476480127	4.334269833842849
8	58.88266187156025	14.217882193971008	17.19419131887108	9.705264615597663
9	54.646168565895294	7.239869323558478	19.98501878945944	18.128943321086787
10	38.89680379859713	21.80752291768517	24.1306596558518	15.165013627865893
11	32.38857986936552	21.49887640920946	30.068590444337666	16.04395327708735
12	26.102975889483147	17.18453955857884	33.72052278969444	22.991961762243573
13	21.406974865557274	25.915186205536333	39.067597991594575	13.610240937311818
14	14.85468855238283	36.161788681003024	29.424230534392787	19.559292232221352
15	9.701697660707055	21.758634653596225	51.8780017499061	16.661665935790616
16	9.70442533209399	24.930706655728006	38.622777734647926	26.742090277530075
17	10.150294693420227	25.079679477629945	35.63933469996664	29.130691128983187
18	14.472604737335736	23.933218211612747	37.79608348353011	23.798093567521406
19	15.782936107445074	28.63656296619576	32.426557447906724	23.153943478452447
20	20.855145961892333	24.693399245064487	35.97861505632641	18.472839736716764
21	15.468834256195485	34.20478937131371	32.47943230863812	17.84694406385269
22	13.971972127394844	23.476438164738763	33.85879474692455	28.692794960941843
23	14.551497386680989	32.640155099591475	30.866119593702855	21.942227920024678
24	17.643837455963844	24.013999248841262	32.55832495798337	25.783838337211524
25	12.755011047069118	32.76227084937589	30.965784509764017	23.51693359379098
26	15.170259149763845	27.05388409914456	34.394677264019705	23.381179487071886
27	18.92584300782422	32.13008055023426	28.139287490269556	20.804788951671956
28	12.776832418164613	29.830233929294565	37.77426211243461	19.61867154010621
29	19.655180372515982	25.140317710770315	30.79897691340902	24.405525003304678
30	16.837915471561928	33.06588165682957	29.255324729278616	20.840878142329895
31	21.5580458962184	30.576776605811617	22.172821062658805	25.692356435311176
32	23.28025564575522	34.38250765321645	21.53181828672862	20.805418414299712
33	17.378414047927286	30.640352331214842	21.5339164954878	30.44731712537007
34	21.210792346573733	28.042560066471257	24.00266892154168	26.743978665413337
35	16.270559823079036	30.68483435690951	22.43677572456394	30.607830095447518
36	22.77437751391637	30.817441150489827	23.297670778456432	23.110510557137374
37	16.45709058177034	34.31263730153568	26.958415600601764	22.27185651609221
38	16.75000052455219	32.14287962366528	20.085942630776106	31.021177221006425
39	20.250652018371916	30.07026901134501	24.07128034796694	25.607798622316132
40	20.7212802430565	21.197153989639045	26.99303604512827	31.08852972217618
41	16.522135053304993	27.424847407767988	22.581761949823438	33.471255589103585
42	22.791373004865743	22.993010866623166	27.550949754194843	26.664666374316248
43	22.613025260335252	27.41288761784065	25.555133582460655	24.418953539363443
44	20.04544720172389	27.30902628426113	27.045910905859667	25.599615608155318
45	16.87316537871619	25.2529915211384	27.09857594571514	30.775267154430264
46	26.59542548526323	25.645986021733247	24.323065399068817	23.435523093934705
47	16.349032830672456	22.342775972152573	34.69283272869951	26.615358468475463
48	19.655180372515982	22.686252746030714	27.08262955914536	30.575937322307944
49	19.12643176520205	18.3075008864932	34.96245255425443	27.60361479405032
50	19.912210945515813	19.76890328726366	32.16742866614771	28.151457101072815
51	18.139854006634536	20.14196480464627	25.873431851228606	35.84474933749058
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	958.0
1	898.0
2	838.0
3	4283.5
4	7729.0
5	5352.5
6	2976.0
7	3054.0
8	3132.0
9	3449.5
10	3767.0
11	3902.5
12	4038.0
13	3944.5
14	3851.0
15	3852.0
16	3853.0
17	3673.0
18	3493.0
19	3439.5
20	3386.0
21	3278.5
22	3171.0
23	3352.5
24	3534.0
25	3550.0
26	3597.5
27	3629.0
28	4321.0
29	5013.0
30	5353.0
31	5693.0
32	5381.0
33	5069.0
34	5335.5
35	5602.0
36	6208.0
37	6814.0
38	8089.5
39	9365.0
40	13273.5
41	17182.0
42	22357.0
43	27532.0
44	32452.0
45	37372.0
46	64999.5
47	92627.0
48	79395.5
49	66164.0
50	59684.5
51	53205.0
52	43149.5
53	33094.0
54	26717.0
55	20340.0
56	16181.5
57	12023.0
58	11013.5
59	10004.0
60	8598.0
61	7192.0
62	6112.0
63	5032.0
64	3542.0
65	2052.0
66	2070.0
67	2088.0
68	1249.5
69	411.0
70	345.5
71	280.0
72	263.5
73	247.0
74	192.5
75	102.5
76	67.0
77	59.5
78	52.0
79	30.5
80	9.0
81	5.5
82	2.0
83	3.5
84	5.0
85	2.5
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	476597.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.916593649906027
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.74366616129349	24.81313001723954
2	9.629262515841889	6.146665177327948
3	3.798128760029193	3.6366999679161967
4	2.0356654280741546	2.598860251000192
5	1.2591943311691627	2.0094596897095682
6	0.7849411582202797	1.503158879160196
7	0.5867585493342564	1.3109133932796861
8	0.4314455495302394	1.1016217829133643
9	0.3323931738400896	0.9547972075311042
>10	2.4653507487154793	17.450792047401855
>50	0.7614117130750975	17.051176616612064
>100	0.15652714659035108	6.669622486431816
>500	0.006632506211455553	1.5768565049490777
>1k	0.006632506211455553	5.961741139600065
>5k	0.0	0.0
>10k+	0.001989751863436666	7.214504838927309
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGC	12690	2.662626915402321	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCG	11123	2.3338376028384564	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTC	10268	2.1544407539283714	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4775	1.0018946825095416	No Hit
GCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	4160	0.872854843819831	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAAACCCAT	3285	0.689261577391381	No Hit
GAACTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCT	3113	0.6531723867334457	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTC	2839	0.595681466731851	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCT	2703	0.5671458276069719	No Hit
CTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGCT	2670	0.5602217387016704	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAAACCCATCGTA	2004	0.4204810353401301	No Hit
CCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	1543	0.32375361154182675	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGC	1071	0.22471815810842286	No Hit
TCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	1000	0.2098208759182286	No Hit
ACTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	924	0.19387448934844323	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	831	0.17436114788804796	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAAACCCATCGT	819	0.17184329737702922	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTT	815	0.17100401387335631	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCG	721	0.15128085153704282	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	630	0.13218715182848403	No Hit
GAACAAGGGAACACAACGAGCGACTGCCACAAAAAAAAGTGCACTCGGGAG	609	0.12778091343420123	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATG	563	0.11812915314196272	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTAT	537	0.11267381036808877	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6357572540322327	0.0
2	0.0	0.0	0.0	2.4605694118930668	0.0
3	0.0	0.0	0.0	3.079540995851841	0.0
4	0.0	0.0	0.0	4.656554699253247	0.0
5	0.0	0.0	0.0	9.091328732660928	0.0
6	0.0	0.0	0.0	10.946774738405823	0.0
7	0.0	0.0	0.0	12.756269972324626	0.0
8	0.0	0.0	0.0	16.657259697396334	0.0
9	0.0	0.0	0.0	17.912198356263257	0.0
10	0.0	0.0	0.0	22.84277911946571	0.0
11	0.0	0.0	0.0	25.270826295591455	0.0
12	0.0	0.0	0.0	30.782401064211484	0.0
13	0.0	0.0	0.0	32.02265226176413	0.0
14	0.0	0.0	0.0	32.434950282943454	0.0
15	0.0	0.0	0.0	33.443978875234215	0.0
16	0.0	0.0	0.0	34.621073989135475	0.0
17	0.0	0.0	0.0	35.6987140078515	0.0
18	8.392835036729145E-4	0.0	0.0	37.75411930834647	0.0
19	8.392835036729145E-4	0.0	0.0	39.24384752736589	0.0
20	8.392835036729145E-4	0.0	0.0	40.17041651542078	0.0
21	8.392835036729145E-4	0.0	0.0	40.830512991059535	0.0
22	0.001678567007345829	0.0	0.0	41.46102472319381	0.0
23	0.001678567007345829	0.0	0.0	41.99313046452244	0.0
24	0.001678567007345829	0.0	0.0	42.415080245994	0.0
25	0.001678567007345829	0.0	0.0	42.745338304689284	0.0
26	0.001678567007345829	0.0	0.0	43.0586008724352	0.0
27	0.001678567007345829	0.0	0.0	43.38906875200641	0.0
28	0.001678567007345829	0.0	0.0	43.68554564967887	0.0
29	0.001678567007345829	0.0	0.0	44.00027696355621	0.0
30	0.001678567007345829	0.0	0.0	44.380472390720044	0.0
31	0.001678567007345829	0.0	0.0	44.735279491897764	0.0
32	0.001678567007345829	0.0	0.0	45.04014922460696	0.0
33	0.001678567007345829	0.0	0.0	45.310188691913716	0.0
34	0.001678567007345829	0.0	0.0	45.603518276447396	0.0
35	0.001678567007345829	0.0	0.0	45.913633531054536	0.0
36	0.001678567007345829	0.0	0.0	46.201507772814345	0.0
37	0.001678567007345829	0.0	0.0	46.49043111895375	0.0
38	0.001678567007345829	0.0	0.0	46.753756318231126	0.0
39	0.001678567007345829	0.0	0.0	47.05338052904236	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.029124E-4	45.000004	25
GTTTGCG	20	7.029124E-4	45.000004	1
AGCGTGA	20	7.029124E-4	45.000004	22
CCTTACT	20	7.029124E-4	45.000004	45
ATCTCGC	20	7.029124E-4	45.000004	14
CTCACGA	20	7.029124E-4	45.000004	24
GTACCCT	20	7.029124E-4	45.000004	43
GGTACAC	20	7.029124E-4	45.000004	8
GTACAAA	20	7.029124E-4	45.000004	42
TACCAAC	20	7.029124E-4	45.000004	20
TTTACAG	40	6.8012014E-9	45.000004	1
CCTAGGC	20	7.029124E-4	45.000004	31
CCTAGCG	20	7.029124E-4	45.000004	20
CGTGCAA	20	7.029124E-4	45.000004	32
GCGAAGC	20	7.029124E-4	45.000004	40
GCGCGAG	20	7.029124E-4	45.000004	37
CCGGCTA	20	7.029124E-4	45.000004	21
AACTTGC	20	7.029124E-4	45.000004	28
AACTTCG	20	7.029124E-4	45.000004	16
CGCATGG	40	6.8012014E-9	45.000004	2
>>END_MODULE