SRR3548264_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548264_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	654949
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGC	15987	2.4409534177470307	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCG	14265	2.178032182658497	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTC	12403	1.893735237400164	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5415	0.82678193263903	No Hit
GCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	4184	0.6388283667888645	No Hit
GAACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCT	3744	0.5716475633980661	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTC	2969	0.45331773924381896	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCT	2607	0.39804626009048033	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2371	0.3620129200899612	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGATGGGTT	1958	0.29895457508905277	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTA	1947	0.2972750550042828	No Hit
CTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGCT	1137	0.17360130330758577	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGATGGGTTCGT	851	0.12993378110356682	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTT	755	0.11527615127284721	No Hit
CCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	734	0.11206979474737727	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTAT	662	0.10107657237433754	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	25	3.88896E-5	45.000004	25
AGCCGTG	25	3.88896E-5	45.000004	31
CGACGGT	25	3.88896E-5	45.000004	28
CGTGCAT	35	1.2108649E-7	45.000004	9
ACCGCAT	35	1.2108649E-7	45.000004	33
CGTTGGA	25	3.88896E-5	45.000004	35
TTCGTCG	35	1.2108649E-7	45.000004	1
GTTAGCG	35	1.2108649E-7	45.000004	1
ATAGACT	35	1.2108649E-7	45.000004	34
CTGACTA	35	1.2108649E-7	45.000004	11
CGACACC	25	3.88896E-5	45.000004	38
TCGATAG	25	3.88896E-5	45.000004	1
ACGGGCC	35	1.2108649E-7	45.000004	5
AAACGTC	25	3.88896E-5	45.000004	12
ACGATAG	50	2.1827873E-11	45.000004	1
CTAACGG	25	3.88896E-5	45.000004	2
TCGGAGT	25	3.88896E-5	45.000004	41
CCGTAGA	25	3.88896E-5	45.000004	42
TCGGACC	35	1.2108649E-7	45.000004	17
TCTACCG	25	3.88896E-5	45.000004	38